ParkinsonCanadaV1, PascalFrancis, Curation, bibRecord, 000516

Dynamic behavior of cells within neurospheres in expanding populations of neural precursors

Identifieur interne : 000516 ( PascalFrancis/Curation ); précédent : 000515; suivant : 000517

Dynamic behavior of cells within neurospheres in expanding populations of neural precursors

Auteurs : Tony Y. Wang [Canada] ; Arindom Sen [Canada] ; Leo A. Behie [Canada] ; Michael S. Kallos [Canada]

Source :

Brain research [ 0006-8993 ] ; 2006.

RBID : Pascal:06-0435848

Descripteurs français

Pascal (Inist)
- Comportement, Formation image, Cellule souche, Cellule précurseur, Système nerveux, Neurosphère.
Wicri :
- topic : Cellule souche.

English descriptors

KwdEn :
- Behavior, Imaging, Nervous system, Neurosphere, Precursor cell, Stem cell.

Abstract

Large-scale expansion of neural stem and progenitor cells will be essential for clinically treating the large number of patients suffering from neurodegenerative disorders such as Parkinson's disease. Other applications of neural stem cell technology include further research in areas such as neural development or drug testing. Neural stem cells can be grown in vitro as tissue aggregates known as neurospheres, and in the current study, experiments were performed to determin6e the spatial arrangement and behavior of the cells within the neurosphere structure. A protocol utilizing sulfonated lipophilic fluorescent dyes was developed to effectively label populations of neural stem and progenitor cells without compromising cell density during culture. Cells retained the labels for at least 7 days. Using the labeling protocol, we discovered that the cells within the neurospheres were mobile and, moreover, the cells on the periphery of the neurospheres could migrate into the center of the neurospheres. Most important, the mixing time of two merging neurospheres was observed to be the same order of magnitude as the neural stem cell doubling time (∼20 h). This study is the first to show that the neurosphere system is dynamic, and these results will serve as a stepping stone to more in-depth studies of the neurosphere microenvironment.

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A08	`01`	`1`	`ENG`	`@1 Dynamic behavior of cells within neurospheres in expanding populations of neural precursors`
A11	`01`	`1`		`@1 WANG (Tony Y.)`
A11	`02`	`1`		`@1 SEN (Arindom)`
A11	`03`	`1`		`@1 BEHIE (Leo A.)`
A11	`04`	`1`		`@1 KALLOS (Michael S.)`
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C01	`01`		`ENG`	@0 Large-scale expansion of neural stem and progenitor cells will be essential for clinically treating the large number of patients suffering from neurodegenerative disorders such as Parkinson's disease. Other applications of neural stem cell technology include further research in areas such as neural development or drug testing. Neural stem cells can be grown in vitro as tissue aggregates known as neurospheres, and in the current study, experiments were performed to determin6e the spatial arrangement and behavior of the cells within the neurosphere structure. A protocol utilizing sulfonated lipophilic fluorescent dyes was developed to effectively label populations of neural stem and progenitor cells without compromising cell density during culture. Cells retained the labels for at least 7 days. Using the labeling protocol, we discovered that the cells within the neurospheres were mobile and, moreover, the cells on the periphery of the neurospheres could migrate into the center of the neurospheres. Most important, the mixing time of two merging neurospheres was observed to be the same order of magnitude as the neural stem cell doubling time (∼20 h). This study is the first to show that the neurosphere system is dynamic, and these results will serve as a stepping stone to more in-depth studies of the neurosphere microenvironment.
C02	`01`	`X`		`@0 002A25A`
C03	`01`	`X`	`FRE`	`@0 Comportement @5 01`
C03	`01`	`X`	`ENG`	`@0 Behavior @5 01`
C03	`01`	`X`	`SPA`	`@0 Conducta @5 01`
C03	`02`	`X`	`FRE`	`@0 Formation image @5 02`
C03	`02`	`X`	`ENG`	`@0 Imaging @5 02`
C03	`02`	`X`	`SPA`	`@0 Formación imagen @5 02`
C03	`03`	`X`	`FRE`	`@0 Cellule souche @5 03`
C03	`03`	`X`	`ENG`	`@0 Stem cell @5 03`
C03	`03`	`X`	`SPA`	`@0 Célula primitiva @5 03`
C03	`04`	`X`	`FRE`	`@0 Cellule précurseur @5 57`
C03	`04`	`X`	`ENG`	`@0 Precursor cell @5 57`
C03	`04`	`X`	`SPA`	`@0 Célula precursor @5 57`
C03	`05`	`X`	`FRE`	`@0 Système nerveux @5 58`
C03	`05`	`X`	`ENG`	`@0 Nervous system @5 58`
C03	`05`	`X`	`SPA`	`@0 Sistema nervioso @5 58`
C03	`06`	`X`	`FRE`	`@0 Neurosphère @4 CD @5 96`
C03	`06`	`X`	`ENG`	`@0 Neurosphere @4 CD @5 96`
N21				`@1 289`

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Le document en format XML

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	La maladie de Parkinson au Canada (serveur d'exploration)
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La maladie de Parkinson au Canada (serveur d'exploration)

Dynamic behavior of cells within neurospheres in expanding populations of neural precursors

Dynamic behavior of cells within neurospheres in expanding populations of neural precursors

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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