Demonstration of the existence of mRNAs encoding N1/cif and N2/cit sodium/nucleoside cotransporters in rat brain.
Identifieur interne : 002570 ( Ncbi/Merge ); précédent : 002569; suivant : 002571Demonstration of the existence of mRNAs encoding N1/cif and N2/cit sodium/nucleoside cotransporters in rat brain.
Auteurs : C M Anderson [Canada] ; W. Xiong ; J D Young ; C E Cass ; F E ParkinsonSource :
- Brain research. Molecular brain research [ 0169-328X ] ; 1996.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Carrier Proteins.
- metabolism : Brain, Purine Nucleosides, RNA, Messenger, Sodium.
- physiology : Biological Transport.
- Animals, Male, Membrane Transport Proteins, Molecular Sequence Data, Rats, Rats, Sprague-Dawley.
Abstract
Nucleoside transport may be involved in the regulation of extracellular levels of adenosine, an inhibitory neuromodulator in the central nervous system. Previous reports have provided functional evidence for Na+-dependent nucleoside transport in rat brain. We isolated total RNA from various regions of rat brain and tested for the presence of mRNA for two recently cloned Na+/nucleoside cotransporters using reverse transcriptase PCR (RT-PCR). Messenger RNA for a pyrimidine-selective Na+/nucleoside cotransporter mRNA (rCNT1) was detected in samples from each brain region tested by RT-PCR amplification of a 309-bp DNA product. Southern blot and sequence analysis confirmed that this product was derived from rCNT1 mRNA. A purine-selective Na+/nucleoside cotransporter mRNA (rCNT2, also termed SPNT) was detected throughout brain by amplifying a 235-bp DNA product, the sequence of which was identical to that published. These experiments demonstrate the presence of both rCNT1 and rCNT2 mRNA in rat brain.
PubMed: 9013795
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Demonstration of the existence of mRNAs encoding N1/cif and N2/cit sodium/nucleoside cotransporters in rat brain.</title><author><name sortKey="Anderson, C M" sort="Anderson, C M" uniqKey="Anderson C" first="C M" last="Anderson">C M Anderson</name><affiliation wicri:level="4"><nlm:affiliation>Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Manitoba, Winnipeg</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Xiong, W" sort="Xiong, W" uniqKey="Xiong W" first="W" last="Xiong">W. Xiong</name></author><author><name sortKey="Young, J D" sort="Young, J D" uniqKey="Young J" first="J D" last="Young">J D Young</name></author><author><name sortKey="Cass, C E" sort="Cass, C E" uniqKey="Cass C" first="C E" last="Cass">C E Cass</name></author><author><name sortKey="Parkinson, F E" sort="Parkinson, F E" uniqKey="Parkinson F" first="F E" last="Parkinson">F E Parkinson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1996">1996</date><idno type="RBID">pubmed:9013795</idno><idno type="pmid">9013795</idno><idno type="wicri:Area/PubMed/Corpus">001853</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001853</idno><idno type="wicri:Area/PubMed/Curation">001853</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001853</idno><idno type="wicri:Area/PubMed/Checkpoint">001853</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001853</idno><idno type="wicri:Area/Ncbi/Merge">002570</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Demonstration of the existence of mRNAs encoding N1/cif and N2/cit sodium/nucleoside cotransporters in rat brain.</title><author><name sortKey="Anderson, C M" sort="Anderson, C M" uniqKey="Anderson C" first="C M" last="Anderson">C M Anderson</name><affiliation wicri:level="4"><nlm:affiliation>Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Manitoba, Winnipeg</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Xiong, W" sort="Xiong, W" uniqKey="Xiong W" first="W" last="Xiong">W. 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Molecular brain research</title><idno type="ISSN">0169-328X</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Biological Transport (physiology)</term><term>Brain (metabolism)</term><term>Carrier Proteins (genetics)</term><term>Male</term><term>Membrane Transport Proteins</term><term>Molecular Sequence Data</term><term>Purine Nucleosides (metabolism)</term><term>RNA, Messenger (metabolism)</term><term>Rats</term><term>Rats, Sprague-Dawley</term><term>Sodium (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Carrier Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Brain</term><term>Purine Nucleosides</term><term>RNA, Messenger</term><term>Sodium</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Biological Transport</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Male</term><term>Membrane Transport Proteins</term><term>Molecular Sequence Data</term><term>Rats</term><term>Rats, Sprague-Dawley</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Nucleoside transport may be involved in the regulation of extracellular levels of adenosine, an inhibitory neuromodulator in the central nervous system. Previous reports have provided functional evidence for Na+-dependent nucleoside transport in rat brain. We isolated total RNA from various regions of rat brain and tested for the presence of mRNA for two recently cloned Na+/nucleoside cotransporters using reverse transcriptase PCR (RT-PCR). Messenger RNA for a pyrimidine-selective Na+/nucleoside cotransporter mRNA (rCNT1) was detected in samples from each brain region tested by RT-PCR amplification of a 309-bp DNA product. Southern blot and sequence analysis confirmed that this product was derived from rCNT1 mRNA. A purine-selective Na+/nucleoside cotransporter mRNA (rCNT2, also termed SPNT) was detected throughout brain by amplifying a 235-bp DNA product, the sequence of which was identical to that published. These experiments demonstrate the presence of both rCNT1 and rCNT2 mRNA in rat brain.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9013795</PMID><DateCreated><Year>1997</Year><Month>04</Month><Day>16</Day></DateCreated><DateCompleted><Year>1997</Year><Month>04</Month><Day>16</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0169-328X</ISSN><JournalIssue CitedMedium="Print"><Volume>42</Volume><Issue>2</Issue><PubDate><Year>1996</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Brain research. Molecular brain research</Title><ISOAbbreviation>Brain Res. Mol. 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A purine-selective Na+/nucleoside cotransporter mRNA (rCNT2, also termed SPNT) was detected throughout brain by amplifying a 235-bp DNA product, the sequence of which was identical to that published. 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MajorTopicYN="N">Sodium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1996</Year><Month>12</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1996</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1996</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9013795</ArticleId><ArticleId IdType="pii">S0169328X96002446</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Manitoba</li></region><settlement><li>Winnipeg</li></settlement><orgName><li>Université du Manitoba</li></orgName></list><tree><noCountry><name sortKey="Cass, C E" sort="Cass, C E" uniqKey="Cass C" first="C E" last="Cass">C E Cass</name><name sortKey="Parkinson, F E" sort="Parkinson, F E" uniqKey="Parkinson F" first="F E" last="Parkinson">F E Parkinson</name><name sortKey="Xiong, W" sort="Xiong, W" uniqKey="Xiong W" first="W" last="Xiong">W. Xiong</name><name sortKey="Young, J D" sort="Young, J D" uniqKey="Young J" first="J D" last="Young">J D Young</name></noCountry><country name="Canada"><region name="Manitoba"><name sortKey="Anderson, C M" sort="Anderson, C M" uniqKey="Anderson C" first="C M" last="Anderson">C M Anderson</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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