A microfluidic phenotype analysis system reveals function of sensory and dopaminergic neuron signaling in C. elegans electrotactic swimming behavior
Identifieur interne : 001566 ( Ncbi/Merge ); précédent : 001565; suivant : 001567A microfluidic phenotype analysis system reveals function of sensory and dopaminergic neuron signaling in C. elegans electrotactic swimming behavior
Auteurs : Sangeena Salam ; Ata Ansari ; Siavash Amon ; Pouya Rezai ; P. Ravi Selvaganapathy ; Ram K. Mishra ; Bhagwati P. GuptaSource :
- Worm [ 2162-4046 ] ; 2013.
Abstract
The nematode (worm)
Url:
DOI: 10.4161/worm.24558
PubMed: 24058875
PubMed Central: 3704449
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electrotactic swimming behavior</title>
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<author><name sortKey="Rezai, Pouya" sort="Rezai, Pouya" uniqKey="Rezai P" first="Pouya" last="Rezai">Pouya Rezai</name>
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<author><name sortKey="Gupta, Bhagwati P" sort="Gupta, Bhagwati P" uniqKey="Gupta B" first="Bhagwati P." last="Gupta">Bhagwati P. Gupta</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A microfluidic phenotype analysis system reveals function of sensory and dopaminergic neuron signaling in <italic><italic>C. elegans</italic>
</italic>
electrotactic swimming behavior</title>
<author><name sortKey="Salam, Sangeena" sort="Salam, Sangeena" uniqKey="Salam S" first="Sangeena" last="Salam">Sangeena Salam</name>
<affiliation><nlm:aff id="A1">Department of Biology; McMaster University; Hamilton, ON Canada</nlm:aff>
<wicri:noCountry code="subfield">ON Canada</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Ansari, Ata" sort="Ansari, Ata" uniqKey="Ansari A" first="Ata" last="Ansari">Ata Ansari</name>
<affiliation><nlm:aff id="A1">Department of Biology; McMaster University; Hamilton, ON Canada</nlm:aff>
<wicri:noCountry code="subfield">ON Canada</wicri:noCountry>
</affiliation>
<affiliation><nlm:aff id="A2">Department of Psychiatry and Behavioral Neuroscience; McMaster University; Hamilton, ON Canada</nlm:aff>
<wicri:noCountry code="subfield">ON Canada</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Amon, Siavash" sort="Amon, Siavash" uniqKey="Amon S" first="Siavash" last="Amon">Siavash Amon</name>
<affiliation><nlm:aff id="A1">Department of Biology; McMaster University; Hamilton, ON Canada</nlm:aff>
<wicri:noCountry code="subfield">ON Canada</wicri:noCountry>
</affiliation>
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<author><name sortKey="Rezai, Pouya" sort="Rezai, Pouya" uniqKey="Rezai P" first="Pouya" last="Rezai">Pouya Rezai</name>
<affiliation><nlm:aff id="A3">Department of Mechanical Engineering; McMaster University; Hamilton, ON Canada</nlm:aff>
<wicri:noCountry code="subfield">ON Canada</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Selvaganapathy, P Ravi" sort="Selvaganapathy, P Ravi" uniqKey="Selvaganapathy P" first="P. Ravi" last="Selvaganapathy">P. Ravi Selvaganapathy</name>
<affiliation><nlm:aff id="A3">Department of Mechanical Engineering; McMaster University; Hamilton, ON Canada</nlm:aff>
<wicri:noCountry code="subfield">ON Canada</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Mishra, Ram K" sort="Mishra, Ram K" uniqKey="Mishra R" first="Ram K." last="Mishra">Ram K. Mishra</name>
<affiliation><nlm:aff id="A2">Department of Psychiatry and Behavioral Neuroscience; McMaster University; Hamilton, ON Canada</nlm:aff>
<wicri:noCountry code="subfield">ON Canada</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Gupta, Bhagwati P" sort="Gupta, Bhagwati P" uniqKey="Gupta B" first="Bhagwati P." last="Gupta">Bhagwati P. Gupta</name>
<affiliation><nlm:aff id="A1">Department of Biology; McMaster University; Hamilton, ON Canada</nlm:aff>
<wicri:noCountry code="subfield">ON Canada</wicri:noCountry>
</affiliation>
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<series><title level="j">Worm</title>
<idno type="ISSN">2162-4046</idno>
<idno type="eISSN">2162-4054</idno>
<imprint><date when="2013">2013</date>
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<front><div type="abstract" xml:lang="en"><p>The nematode (worm) <italic>C. elegans</italic>
is a leading multicellular animal model to study neuronal-basis of behavior. Worms respond to a wide range of stimuli and exhibit characteristic movement patterns. Here we describe the use of a microfluidics setup to probe neuronal activity that relies on the innate response of <italic>C. elegans</italic>
to swim toward the cathode in the presence of a DC electric field (termed “electrotaxis”). Using this setup, we examined mutants affecting sensory and dopaminergic neurons and found that their electrotactic responses were defective. Such animals moved with reduced speed (35–80% slower than controls) with intermittent pauses, abnormal turning and slower body bends. A similar phenotype was observed in worms treated with neurotoxins 6-OHDA (6- hydroxy dopamine), MPTP (1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine) and rotenone (20–60% slower). We also found that neurotoxin effects could be suppressed by pre-exposing worms to a known neuroprotective compound acetaminophen. Collectively, these results show that microfluidic electrotaxis can identify alterations in dopamine and amphid neuronal signaling based on swimming responses of <italic>C. elegans</italic>
. Further characterization has revealed that the electrotactic swimming response is highly sensitive and reliable in detecting neuronal abnormalities. Thus, our microfluidics setup could be used to dissect neuronal function and toxin-induced neurodegeneration. Among other applications, the setup promises to facilitate genetic and chemical screenings to identify factors that mediate neuronal signaling and neuroprotection.</p>
</div>
</front>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Worm</journal-id>
<journal-id journal-id-type="iso-abbrev">Worm</journal-id>
<journal-id journal-id-type="publisher-id">WORM</journal-id>
<journal-title-group><journal-title>Worm</journal-title>
</journal-title-group>
<issn pub-type="ppub">2162-4046</issn>
<issn pub-type="epub">2162-4054</issn>
<publisher><publisher-name>Landes Bioscience</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">24058875</article-id>
<article-id pub-id-type="pmc">3704449</article-id>
<article-id pub-id-type="publisher-id">2012WORM094RR</article-id>
<article-id pub-id-type="doi">10.4161/worm.24558</article-id>
<article-id pub-id-type="pii">24558</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Paper</subject>
</subj-group>
</article-categories>
<title-group><article-title>A microfluidic phenotype analysis system reveals function of sensory and dopaminergic neuron signaling in <italic><italic>C. elegans</italic>
</italic>
electrotactic swimming behavior</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Salam</surname>
<given-names>Sangeena</given-names>
</name>
<xref ref-type="aff" rid="A1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ansari</surname>
<given-names>Ata</given-names>
</name>
<xref ref-type="aff" rid="A1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="A2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Amon</surname>
<given-names>Siavash</given-names>
</name>
<xref ref-type="aff" rid="A1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Rezai</surname>
<given-names>Pouya</given-names>
</name>
<xref ref-type="aff" rid="A3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Selvaganapathy</surname>
<given-names>P. Ravi</given-names>
</name>
<xref ref-type="aff" rid="A3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Mishra</surname>
<given-names>Ram K.</given-names>
</name>
<xref ref-type="aff" rid="A2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Gupta</surname>
<given-names>Bhagwati P.</given-names>
</name>
<xref ref-type="aff" rid="A1"><sup>1</sup>
</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
</contrib>
<aff id="A1"><label>1</label>
Department of Biology; McMaster University; Hamilton, ON Canada</aff>
<aff id="A2"><label>2</label>
Department of Psychiatry and Behavioral Neuroscience; McMaster University; Hamilton, ON Canada</aff>
<aff id="A3"><label>3</label>
Department of Mechanical Engineering; McMaster University; Hamilton, ON Canada</aff>
</contrib-group>
<author-notes><corresp id="cor1"><label>*</label>
Correspondence to: Bhagwati P. Gupta, Email: <email xlink:href="guptab@mcmaster.ca">guptab@mcmaster.ca</email>
</corresp>
</author-notes>
<pub-date pub-type="ppub"><day>01</day>
<month>4</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="epub"><day>18</day>
<month>4</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>18</day>
<month>4</month>
<year>2013</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
. </pmc-comment>
<volume>2</volume>
<issue>2</issue>
<elocation-id>e24558</elocation-id>
<history><date date-type="received"><day>14</day>
<month>2</month>
<year>2013</year>
</date>
<date date-type="rev-recd"><day>02</day>
<month>4</month>
<year>2013</year>
</date>
<date date-type="accepted"><day>04</day>
<month>4</month>
<year>2013</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2013 Landes Bioscience</copyright-statement>
<copyright-year>2013</copyright-year>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/"><license-p>This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.</license-p>
</license>
</permissions>
<abstract><p>The nematode (worm) <italic>C. elegans</italic>
is a leading multicellular animal model to study neuronal-basis of behavior. Worms respond to a wide range of stimuli and exhibit characteristic movement patterns. Here we describe the use of a microfluidics setup to probe neuronal activity that relies on the innate response of <italic>C. elegans</italic>
to swim toward the cathode in the presence of a DC electric field (termed “electrotaxis”). Using this setup, we examined mutants affecting sensory and dopaminergic neurons and found that their electrotactic responses were defective. Such animals moved with reduced speed (35–80% slower than controls) with intermittent pauses, abnormal turning and slower body bends. A similar phenotype was observed in worms treated with neurotoxins 6-OHDA (6- hydroxy dopamine), MPTP (1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine) and rotenone (20–60% slower). We also found that neurotoxin effects could be suppressed by pre-exposing worms to a known neuroprotective compound acetaminophen. Collectively, these results show that microfluidic electrotaxis can identify alterations in dopamine and amphid neuronal signaling based on swimming responses of <italic>C. elegans</italic>
. Further characterization has revealed that the electrotactic swimming response is highly sensitive and reliable in detecting neuronal abnormalities. Thus, our microfluidics setup could be used to dissect neuronal function and toxin-induced neurodegeneration. Among other applications, the setup promises to facilitate genetic and chemical screenings to identify factors that mediate neuronal signaling and neuroprotection.</p>
</abstract>
<kwd-group kwd-group-type="author"><title>Keywords: </title>
<kwd><italic>C. elegans</italic>
</kwd>
<kwd>nematode</kwd>
<kwd>microfluidics</kwd>
<kwd>electrotaxis</kwd>
<kwd>neuronal signaling</kwd>
<kwd>dopamine signaling</kwd>
<kwd>neurodegeneration</kwd>
<kwd>neurotoxin</kwd>
<kwd>6-OHDA</kwd>
<kwd>MPTP</kwd>
<kwd>Rotenone</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Amon, Siavash" sort="Amon, Siavash" uniqKey="Amon S" first="Siavash" last="Amon">Siavash Amon</name>
<name sortKey="Ansari, Ata" sort="Ansari, Ata" uniqKey="Ansari A" first="Ata" last="Ansari">Ata Ansari</name>
<name sortKey="Gupta, Bhagwati P" sort="Gupta, Bhagwati P" uniqKey="Gupta B" first="Bhagwati P." last="Gupta">Bhagwati P. Gupta</name>
<name sortKey="Mishra, Ram K" sort="Mishra, Ram K" uniqKey="Mishra R" first="Ram K." last="Mishra">Ram K. Mishra</name>
<name sortKey="Rezai, Pouya" sort="Rezai, Pouya" uniqKey="Rezai P" first="Pouya" last="Rezai">Pouya Rezai</name>
<name sortKey="Salam, Sangeena" sort="Salam, Sangeena" uniqKey="Salam S" first="Sangeena" last="Salam">Sangeena Salam</name>
<name sortKey="Selvaganapathy, P Ravi" sort="Selvaganapathy, P Ravi" uniqKey="Selvaganapathy P" first="P. Ravi" last="Selvaganapathy">P. Ravi Selvaganapathy</name>
</noCountry>
</tree>
</affiliations>
</record>
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