Ethanol alters glutamate but not adenosine uptake in rat astrocytes: evidence for protein kinase C involvement.
Identifieur interne : 000217 ( Ncbi/Merge ); précédent : 000216; suivant : 000218Ethanol alters glutamate but not adenosine uptake in rat astrocytes: evidence for protein kinase C involvement.
Auteurs : Timothy Othman [Canada] ; Christopher J D. Sinclair ; Norman Haughey ; Jonathan D. Geiger ; Fiona E. ParkinsonSource :
- Neurochemical research [ 0364-3190 ] ; 2002.
English descriptors
- KwdEn :
- Adenosine (metabolism), Adenosine Triphosphate (metabolism), Alkaloids, Animals, Astrocytes (drug effects), Astrocytes (metabolism), Benzophenanthridines, Biological Transport (drug effects), Cells, Cultured, Cerebral Cortex (drug effects), Cerebral Cortex (embryology), Cerebral Cortex (metabolism), Embryo, Mammalian, Ethanol (pharmacology), Glutamic Acid (metabolism), Kinetics, Phenanthridines (pharmacology), Protein Kinase C (metabolism), Rats, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction, Tetradecanoylphorbol Acetate (pharmacology).
- MESH :
- chemical , metabolism : Adenosine, Adenosine Triphosphate, Glutamic Acid, Protein Kinase C.
- chemical , pharmacology : Ethanol, Phenanthridines, Tetradecanoylphorbol Acetate.
- chemical : Alkaloids, Benzophenanthridines.
- drug effects : Astrocytes, Biological Transport, Cerebral Cortex.
- embryology : Cerebral Cortex.
- metabolism : Astrocytes, Cerebral Cortex.
- Animals, Cells, Cultured, Embryo, Mammalian, Kinetics, Rats, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Glutamate is the primary excitatory neurotransmitter in brain. By stimulating neuronal activity, glutamate increases cellular energy utilization, enhances ATP hydrolysis and promotes the formation of adenosine. Adenosine has receptor-mediated effects that reduce or oppose the excitatory effects of glutamate. As a possible mechanism for ethanol's ability to inhibit excitatory effects of glutamate and enhance inhibitory effects of adenosine, we tested the hypothesis that ethanol promotes [3H]glutamate uptake and inhibits [3H]adenosine uptake. Using primary cultures of rat astrocytes, we found that acute treatment with ethanol (50 mM, 30 min) inhibited [3H]glutamate uptake and reduced protein kinase C (PKC)-induced stimulation of [3H]glutamate uptake. Prolonged treatment (50 mM, 3 day) with ethanol, however, increased both [3H]glutamate uptake and PKC activity. Contrary to other cell types, neither acute or chronic ethanol exposure affected [3H]adenosine uptake in astrocytes. These data indicate that in rat cortical astrocytes ethanol affects [3H]glutamate uptake but not [3H]adenosine uptake by affecting PKC modulation of transporter activity.
PubMed: 11958530
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Sinclair</name></author><author><name sortKey="Haughey, Norman" sort="Haughey, Norman" uniqKey="Haughey N" first="Norman" last="Haughey">Norman Haughey</name></author><author><name sortKey="Geiger, Jonathan D" sort="Geiger, Jonathan D" uniqKey="Geiger J" first="Jonathan D" last="Geiger">Jonathan D. Geiger</name></author><author><name sortKey="Parkinson, Fiona E" sort="Parkinson, Fiona E" uniqKey="Parkinson F" first="Fiona E" last="Parkinson">Fiona E. Parkinson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2002">2002</date><idno type="RBID">pubmed:11958530</idno><idno type="pmid">11958530</idno><idno type="wicri:Area/PubMed/Corpus">001521</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001521</idno><idno type="wicri:Area/PubMed/Curation">001521</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001521</idno><idno type="wicri:Area/PubMed/Checkpoint">001521</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001521</idno><idno type="wicri:Area/Ncbi/Merge">000217</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Ethanol alters glutamate but not adenosine uptake in rat astrocytes: evidence for protein kinase C involvement.</title><author><name sortKey="Othman, Timothy" sort="Othman, Timothy" uniqKey="Othman T" first="Timothy" last="Othman">Timothy Othman</name><affiliation wicri:level="4"><nlm:affiliation>Department of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Pharmacology and Therapeutics, University of Manitoba, Winnipeg</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Sinclair, Christopher J D" sort="Sinclair, Christopher J D" uniqKey="Sinclair C" first="Christopher J D" last="Sinclair">Christopher J D. Sinclair</name></author><author><name sortKey="Haughey, Norman" sort="Haughey, Norman" uniqKey="Haughey N" first="Norman" last="Haughey">Norman Haughey</name></author><author><name sortKey="Geiger, Jonathan D" sort="Geiger, Jonathan D" uniqKey="Geiger J" first="Jonathan D" last="Geiger">Jonathan D. Geiger</name></author><author><name sortKey="Parkinson, Fiona E" sort="Parkinson, Fiona E" uniqKey="Parkinson F" first="Fiona E" last="Parkinson">Fiona E. Parkinson</name></author></analytic><series><title level="j">Neurochemical research</title><idno type="ISSN">0364-3190</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenosine (metabolism)</term><term>Adenosine Triphosphate (metabolism)</term><term>Alkaloids</term><term>Animals</term><term>Astrocytes (drug effects)</term><term>Astrocytes (metabolism)</term><term>Benzophenanthridines</term><term>Biological Transport (drug effects)</term><term>Cells, Cultured</term><term>Cerebral Cortex (drug effects)</term><term>Cerebral Cortex (embryology)</term><term>Cerebral Cortex (metabolism)</term><term>Embryo, Mammalian</term><term>Ethanol (pharmacology)</term><term>Glutamic Acid (metabolism)</term><term>Kinetics</term><term>Phenanthridines (pharmacology)</term><term>Protein Kinase C (metabolism)</term><term>Rats</term><term>Rats, Sprague-Dawley</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Tetradecanoylphorbol Acetate (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Adenosine</term><term>Adenosine Triphosphate</term><term>Glutamic Acid</term><term>Protein Kinase C</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Ethanol</term><term>Phenanthridines</term><term>Tetradecanoylphorbol Acetate</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Alkaloids</term><term>Benzophenanthridines</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Astrocytes</term><term>Biological Transport</term><term>Cerebral Cortex</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Cerebral Cortex</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Astrocytes</term><term>Cerebral Cortex</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Embryo, Mammalian</term><term>Kinetics</term><term>Rats</term><term>Rats, Sprague-Dawley</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Glutamate is the primary excitatory neurotransmitter in brain. By stimulating neuronal activity, glutamate increases cellular energy utilization, enhances ATP hydrolysis and promotes the formation of adenosine. Adenosine has receptor-mediated effects that reduce or oppose the excitatory effects of glutamate. As a possible mechanism for ethanol's ability to inhibit excitatory effects of glutamate and enhance inhibitory effects of adenosine, we tested the hypothesis that ethanol promotes [3H]glutamate uptake and inhibits [3H]adenosine uptake. Using primary cultures of rat astrocytes, we found that acute treatment with ethanol (50 mM, 30 min) inhibited [3H]glutamate uptake and reduced protein kinase C (PKC)-induced stimulation of [3H]glutamate uptake. Prolonged treatment (50 mM, 3 day) with ethanol, however, increased both [3H]glutamate uptake and PKC activity. Contrary to other cell types, neither acute or chronic ethanol exposure affected [3H]adenosine uptake in astrocytes. These data indicate that in rat cortical astrocytes ethanol affects [3H]glutamate uptake but not [3H]adenosine uptake by affecting PKC modulation of transporter activity.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11958530</PMID><DateCreated><Year>2002</Year><Month>04</Month><Day>17</Day></DateCreated><DateCompleted><Year>2002</Year><Month>10</Month><Day>04</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0364-3190</ISSN><JournalIssue CitedMedium="Print"><Volume>27</Volume><Issue>4</Issue><PubDate><Year>2002</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Neurochemical research</Title><ISOAbbreviation>Neurochem. Res.</ISOAbbreviation></Journal><ArticleTitle>Ethanol alters glutamate but not adenosine uptake in rat astrocytes: evidence for protein kinase C involvement.</ArticleTitle><Pagination><MedlinePgn>289-96</MedlinePgn></Pagination><Abstract><AbstractText>Glutamate is the primary excitatory neurotransmitter in brain. By stimulating neuronal activity, glutamate increases cellular energy utilization, enhances ATP hydrolysis and promotes the formation of adenosine. Adenosine has receptor-mediated effects that reduce or oppose the excitatory effects of glutamate. As a possible mechanism for ethanol's ability to inhibit excitatory effects of glutamate and enhance inhibitory effects of adenosine, we tested the hypothesis that ethanol promotes [3H]glutamate uptake and inhibits [3H]adenosine uptake. Using primary cultures of rat astrocytes, we found that acute treatment with ethanol (50 mM, 30 min) inhibited [3H]glutamate uptake and reduced protein kinase C (PKC)-induced stimulation of [3H]glutamate uptake. Prolonged treatment (50 mM, 3 day) with ethanol, however, increased both [3H]glutamate uptake and PKC activity. Contrary to other cell types, neither acute or chronic ethanol exposure affected [3H]adenosine uptake in astrocytes. These data indicate that in rat cortical astrocytes ethanol affects [3H]glutamate uptake but not [3H]adenosine uptake by affecting PKC modulation of transporter activity.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Othman</LastName><ForeName>Timothy</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sinclair</LastName><ForeName>Christopher J D</ForeName><Initials>CJ</Initials></Author><Author ValidYN="Y"><LastName>Haughey</LastName><ForeName>Norman</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Geiger</LastName><ForeName>Jonathan D</ForeName><Initials>JD</Initials></Author><Author ValidYN="Y"><LastName>Parkinson</LastName><ForeName>Fiona E</ForeName><Initials>FE</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Neurochem Res</MedlineTA><NlmUniqueID>7613461</NlmUniqueID><ISSNLinking>0364-3190</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000470">Alkaloids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D053119">Benzophenanthridines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010617">Phenanthridines</NameOfSubstance></Chemical><Chemical><RegistryNumber>3K9958V90M</RegistryNumber><NameOfSubstance UI="D000431">Ethanol</NameOfSubstance></Chemical><Chemical><RegistryNumber>3KX376GY7L</RegistryNumber><NameOfSubstance UI="D018698">Glutamic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>8L70Q75FXE</RegistryNumber><NameOfSubstance UI="D000255">Adenosine Triphosphate</NameOfSubstance></Chemical><Chemical><RegistryNumber>E3B045W6X0</RegistryNumber><NameOfSubstance UI="C016299">chelerythrine</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.11.13</RegistryNumber><NameOfSubstance UI="D011493">Protein Kinase C</NameOfSubstance></Chemical><Chemical><RegistryNumber>K72T3FS567</RegistryNumber><NameOfSubstance UI="D000241">Adenosine</NameOfSubstance></Chemical><Chemical><RegistryNumber>NI40JAQ945</RegistryNumber><NameOfSubstance UI="D013755">Tetradecanoylphorbol Acetate</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000241" MajorTopicYN="N">Adenosine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000255" MajorTopicYN="N">Adenosine Triphosphate</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000470" MajorTopicYN="N">Alkaloids</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001253" MajorTopicYN="N">Astrocytes</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053119" MajorTopicYN="N">Benzophenanthridines</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001692" MajorTopicYN="N">Biological Transport</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002540" 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MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011493" MajorTopicYN="N">Protein Kinase C</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051381" MajorTopicYN="N">Rats</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017207" MajorTopicYN="N">Rats, Sprague-Dawley</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013755" MajorTopicYN="N">Tetradecanoylphorbol Acetate</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2002</Year><Month>4</Month><Day>18</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2002</Year><Month>10</Month><Day>9</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2002</Year><Month>4</Month><Day>18</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11958530</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Manitoba</li></region><settlement><li>Winnipeg</li></settlement><orgName><li>Université du Manitoba</li></orgName></list><tree><noCountry><name sortKey="Geiger, Jonathan D" sort="Geiger, Jonathan D" uniqKey="Geiger J" first="Jonathan D" last="Geiger">Jonathan D. Geiger</name><name sortKey="Haughey, Norman" sort="Haughey, Norman" uniqKey="Haughey N" first="Norman" last="Haughey">Norman Haughey</name><name sortKey="Parkinson, Fiona E" sort="Parkinson, Fiona E" uniqKey="Parkinson F" first="Fiona E" last="Parkinson">Fiona E. Parkinson</name><name sortKey="Sinclair, Christopher J D" sort="Sinclair, Christopher J D" uniqKey="Sinclair C" first="Christopher J D" last="Sinclair">Christopher J D. Sinclair</name></noCountry><country name="Canada"><region name="Manitoba"><name sortKey="Othman, Timothy" sort="Othman, Timothy" uniqKey="Othman T" first="Timothy" last="Othman">Timothy Othman</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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