Expression of dopamine receptors in the subthalamic nucleus of the rat : Characterization using reverse transcriptase-polymerase chain reaction and autoradiography
Identifieur interne : 003E10 ( Main/Merge ); précédent : 003E09; suivant : 003E11Expression of dopamine receptors in the subthalamic nucleus of the rat : Characterization using reverse transcriptase-polymerase chain reaction and autoradiography
Auteurs : G. Flores [Mexique, Canada] ; J. J. Liang [Mexique, Canada] ; A. Sierra [Mexique] ; D. Martinez-Fong [Mexique] ; R. Quirion [Mexique, Canada] ; J. Aceves [Mexique] ; L. K. Srivastava [Mexique]Source :
- Neuroscience [ 0306-4522 ] ; 1999.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
We analysed the expression of dopamine receptor subtypes in the subthalamic nucleus by means of reverse transcriptase-polymerase chain reaction. We also studied, using autoradiography, all pharmacologically characterized dopamine receptors in four subregions of the subthalamic nucleus. For comparison, dopamine receptor subtypes were also evaluated in brain regions where they are more abundant and well characterized. The radioligands used were: [3H]SCH-23390, [3H]emonapride and [3H]2-dipropylamino-7-hydroxy-1,2,3,4-tetrahydronaphthalene for dopamine D1, D2 and D3 receptors, respectively; and [3H]YM-09151-2 in the presence of raclopride for dopamine D4 receptors. Finally, we also evaluated the effect of unilateral 6-hydroxydopamine injection into the medial forebrain bundle on dopamine receptor levels expressed in the ipsilateral subthalamic nucleus. The lesion was estimated by decrease in the binding of [3H]WIN-35428, a specific dopamine transporter label. D1, D2 and D3 receptor messenger RNAs and binding sites were present in the subthalamic nucleus, but no messenger RNA for D4 receptors was found, although specific binding sites for these receptors were observed. As compared to the intact side, the 6-hydroxydopamine lesion did not change D1 receptors, increased D2 receptors, and decreased D3 receptors and the dopamine transporter. The results suggest that postsynaptic D1, D2 or D3 receptors can mediate the effect of dopamine on subthalamic nucleus neuronal activity. D4 receptors would mediate exclusively presynaptic effects. These results reinforce the idea that dopamine receptors in the subthalamic nucleus may play an important role in the physiology of the basal ganglia and in the pathophysiology of Parkinson's disease.
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000D68
- to stream PascalFrancis, to step Curation: 001155
- to stream PascalFrancis, to step Checkpoint: 000C57
Links to Exploration step
Pascal:99-0278353Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Expression of dopamine receptors in the subthalamic nucleus of the rat : Characterization using reverse transcriptase-polymerase chain reaction and autoradiography</title><author><name sortKey="Flores, G" sort="Flores, G" uniqKey="Flores G" first="G." last="Flores">G. Flores</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto de Fisiologia, Universidad Aut6noma de Puebla, Apartado postal 406</s1><s2>72000 Puebla</s2><s3>MEX</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Mexique</country><wicri:noRegion>72000 Puebla</wicri:noRegion></affiliation><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>McGill Center for Research in Schizophrenia, Douglas Hospital Research Center, Department of Psychiatry, McGill University</s1><s2>Montreal, Quebec, H4H 1R3</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Montréal</settlement><region type="state">Québec</region></placeName><orgName type="university">Université McGill</orgName></affiliation></author><author><name sortKey="Liang, J J" sort="Liang, J J" uniqKey="Liang J" first="J. J." last="Liang">J. J. Liang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto de Fisiologia, Universidad Aut6noma de Puebla, Apartado postal 406</s1><s2>72000 Puebla</s2><s3>MEX</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Mexique</country><wicri:noRegion>72000 Puebla</wicri:noRegion></affiliation><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>McGill Center for Research in Schizophrenia, Douglas Hospital Research Center, Department of Psychiatry, McGill University</s1><s2>Montreal, Quebec, H4H 1R3</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Montréal</settlement><region type="state">Québec</region></placeName><orgName type="university">Université McGill</orgName></affiliation></author><author><name sortKey="Sierra, A" sort="Sierra, A" uniqKey="Sierra A" first="A." last="Sierra">A. Sierra</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Departamento de Fisiologia, Biofísica y Neurociencias, Centro de Investigaci6n y de Estudios Avanzados del Instituto Politécnico Nacional de Mexico, Apartado postal 14-740</s1><s2>07000 Mexico City, D.F.</s2><s3>MEX</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Mexique</country><wicri:noRegion>07000 Mexico City, D.F.</wicri:noRegion></affiliation></author><author><name sortKey="Martinez Fong, D" sort="Martinez Fong, D" uniqKey="Martinez Fong D" first="D." last="Martinez-Fong">D. Martinez-Fong</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Departamento de Fisiologia, Biofísica y Neurociencias, Centro de Investigaci6n y de Estudios Avanzados del Instituto Politécnico Nacional de Mexico, Apartado postal 14-740</s1><s2>07000 Mexico City, D.F.</s2><s3>MEX</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Mexique</country><wicri:noRegion>07000 Mexico City, D.F.</wicri:noRegion></affiliation></author><author><name sortKey="Quirion, R" sort="Quirion, R" uniqKey="Quirion R" first="R." last="Quirion">R. Quirion</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto de Fisiologia, Universidad Aut6noma de Puebla, Apartado postal 406</s1><s2>72000 Puebla</s2><s3>MEX</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Mexique</country><wicri:noRegion>72000 Puebla</wicri:noRegion></affiliation><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>McGill Center for Research in Schizophrenia, Douglas Hospital Research Center, Department of Psychiatry, McGill University</s1><s2>Montreal, Quebec, H4H 1R3</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Montréal</settlement><region type="state">Québec</region></placeName><orgName type="university">Université McGill</orgName></affiliation></author><author><name sortKey="Aceves, J" sort="Aceves, J" uniqKey="Aceves J" first="J." last="Aceves">J. Aceves</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Departamento de Fisiologia, Biofísica y Neurociencias, Centro de Investigaci6n y de Estudios Avanzados del Instituto Politécnico Nacional de Mexico, Apartado postal 14-740</s1><s2>07000 Mexico City, D.F.</s2><s3>MEX</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Mexique</country><wicri:noRegion>07000 Mexico City, D.F.</wicri:noRegion></affiliation></author><author><name sortKey="Srivastava, L K" sort="Srivastava, L K" uniqKey="Srivastava L" first="L. K." last="Srivastava">L. K. Srivastava</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto de Fisiologia, Universidad Aut6noma de Puebla, Apartado postal 406</s1><s2>72000 Puebla</s2><s3>MEX</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Mexique</country><wicri:noRegion>72000 Puebla</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">99-0278353</idno><date when="1999">1999</date><idno type="stanalyst">PASCAL 99-0278353 INIST</idno><idno type="RBID">Pascal:99-0278353</idno><idno type="wicri:Area/PascalFrancis/Corpus">000D68</idno><idno type="wicri:Area/PascalFrancis/Curation">001155</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000C57</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000C57</idno><idno type="wicri:doubleKey">0306-4522:1999:Flores G:expression:of:dopamine</idno><idno type="wicri:Area/Main/Merge">003E10</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Expression of dopamine receptors in the subthalamic nucleus of the rat : Characterization using reverse transcriptase-polymerase chain reaction and autoradiography</title><author><name sortKey="Flores, G" sort="Flores, G" uniqKey="Flores G" first="G." last="Flores">G. Flores</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto de Fisiologia, Universidad Aut6noma de Puebla, Apartado postal 406</s1><s2>72000 Puebla</s2><s3>MEX</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Mexique</country><wicri:noRegion>72000 Puebla</wicri:noRegion></affiliation><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>McGill Center for Research in Schizophrenia, Douglas Hospital Research Center, Department of Psychiatry, McGill University</s1><s2>Montreal, Quebec, H4H 1R3</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Montréal</settlement><region type="state">Québec</region></placeName><orgName type="university">Université McGill</orgName></affiliation></author><author><name sortKey="Liang, J J" sort="Liang, J J" uniqKey="Liang J" first="J. J." last="Liang">J. J. Liang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto de Fisiologia, Universidad Aut6noma de Puebla, Apartado postal 406</s1><s2>72000 Puebla</s2><s3>MEX</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Mexique</country><wicri:noRegion>72000 Puebla</wicri:noRegion></affiliation><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>McGill Center for Research in Schizophrenia, Douglas Hospital Research Center, Department of Psychiatry, McGill University</s1><s2>Montreal, Quebec, H4H 1R3</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Montréal</settlement><region type="state">Québec</region></placeName><orgName type="university">Université McGill</orgName></affiliation></author><author><name sortKey="Sierra, A" sort="Sierra, A" uniqKey="Sierra A" first="A." last="Sierra">A. Sierra</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Departamento de Fisiologia, Biofísica y Neurociencias, Centro de Investigaci6n y de Estudios Avanzados del Instituto Politécnico Nacional de Mexico, Apartado postal 14-740</s1><s2>07000 Mexico City, D.F.</s2><s3>MEX</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Mexique</country><wicri:noRegion>07000 Mexico City, D.F.</wicri:noRegion></affiliation></author><author><name sortKey="Martinez Fong, D" sort="Martinez Fong, D" uniqKey="Martinez Fong D" first="D." last="Martinez-Fong">D. Martinez-Fong</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Departamento de Fisiologia, Biofísica y Neurociencias, Centro de Investigaci6n y de Estudios Avanzados del Instituto Politécnico Nacional de Mexico, Apartado postal 14-740</s1><s2>07000 Mexico City, D.F.</s2><s3>MEX</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Mexique</country><wicri:noRegion>07000 Mexico City, D.F.</wicri:noRegion></affiliation></author><author><name sortKey="Quirion, R" sort="Quirion, R" uniqKey="Quirion R" first="R." last="Quirion">R. Quirion</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto de Fisiologia, Universidad Aut6noma de Puebla, Apartado postal 406</s1><s2>72000 Puebla</s2><s3>MEX</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Mexique</country><wicri:noRegion>72000 Puebla</wicri:noRegion></affiliation><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>McGill Center for Research in Schizophrenia, Douglas Hospital Research Center, Department of Psychiatry, McGill University</s1><s2>Montreal, Quebec, H4H 1R3</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Montréal</settlement><region type="state">Québec</region></placeName><orgName type="university">Université McGill</orgName></affiliation></author><author><name sortKey="Aceves, J" sort="Aceves, J" uniqKey="Aceves J" first="J." last="Aceves">J. Aceves</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Departamento de Fisiologia, Biofísica y Neurociencias, Centro de Investigaci6n y de Estudios Avanzados del Instituto Politécnico Nacional de Mexico, Apartado postal 14-740</s1><s2>07000 Mexico City, D.F.</s2><s3>MEX</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Mexique</country><wicri:noRegion>07000 Mexico City, D.F.</wicri:noRegion></affiliation></author><author><name sortKey="Srivastava, L K" sort="Srivastava, L K" uniqKey="Srivastava L" first="L. K." last="Srivastava">L. K. Srivastava</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto de Fisiologia, Universidad Aut6noma de Puebla, Apartado postal 406</s1><s2>72000 Puebla</s2><s3>MEX</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Mexique</country><wicri:noRegion>72000 Puebla</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Neuroscience</title><title level="j" type="abbreviated">Neuroscience</title><idno type="ISSN">0306-4522</idno><imprint><date when="1999">1999</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Neuroscience</title><title level="j" type="abbreviated">Neuroscience</title><idno type="ISSN">0306-4522</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animal</term><term>Autoradiography</term><term>Basal ganglion</term><term>Carrier protein</term><term>Characterization</term><term>Dopamine</term><term>Dopamine receptor</term><term>Gene expression</term><term>Locus niger</term><term>Parkinson disease</term><term>Pathophysiology</term><term>Polymerase chain reaction</term><term>Rat</term><term>Subthalamic nucleus</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Expression génique</term><term>Récepteur dopaminergique</term><term>Noyau sousthalamique</term><term>Caractérisation</term><term>Autoradiographie</term><term>Réaction chaîne polymérase</term><term>Noyau gris central</term><term>Locus niger</term><term>Parkinson maladie</term><term>Protéine transport</term><term>Dopamine</term><term>Physiopathologie</term><term>Animal</term><term>Rat</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We analysed the expression of dopamine receptor subtypes in the subthalamic nucleus by means of reverse transcriptase-polymerase chain reaction. We also studied, using autoradiography, all pharmacologically characterized dopamine receptors in four subregions of the subthalamic nucleus. For comparison, dopamine receptor subtypes were also evaluated in brain regions where they are more abundant and well characterized. The radioligands used were: [<sup>3</sup>H]SCH-23390, [<sup>3</sup>H]emonapride and [3H]2-dipropylamino-7-hydroxy-1,2,3,4-tetrahydronaphthalene for dopamine D<sub>1</sub>, D<sub>2</sub> and D<sub>3</sub> receptors, respectively; and [<sup>3</sup>H]YM-09151-2 in the presence of raclopride for dopamine D<sub>4</sub> receptors. Finally, we also evaluated the effect of unilateral 6-hydroxydopamine injection into the medial forebrain bundle on dopamine receptor levels expressed in the ipsilateral subthalamic nucleus. The lesion was estimated by decrease in the binding of [<sup>3</sup>H]WIN-35428, a specific dopamine transporter label. D<sub>1</sub>, D<sub>2</sub> and D<sub>3</sub> receptor messenger RNAs and binding sites were present in the subthalamic nucleus, but no messenger RNA for D<sub>4</sub> receptors was found, although specific binding sites for these receptors were observed. As compared to the intact side, the 6-hydroxydopamine lesion did not change D<sub>1</sub> receptors, increased D<sub>2</sub> receptors, and decreased D<sub>3</sub> receptors and the dopamine transporter. The results suggest that postsynaptic D<sub>1</sub>, D<sub>2</sub> or D<sub>3</sub> receptors can mediate the effect of dopamine on subthalamic nucleus neuronal activity. D<sub>4</sub> receptors would mediate exclusively presynaptic effects. These results reinforce the idea that dopamine receptors in the subthalamic nucleus may play an important role in the physiology of the basal ganglia and in the pathophysiology of Parkinson's disease.</div></front></TEI><affiliations><list><country><li>Canada</li><li>Mexique</li></country><region><li>Québec</li></region><settlement><li>Montréal</li></settlement><orgName><li>Université McGill</li></orgName></list><tree><country name="Mexique"><noRegion><name sortKey="Flores, G" sort="Flores, G" uniqKey="Flores G" first="G." last="Flores">G. Flores</name></noRegion><name sortKey="Aceves, J" sort="Aceves, J" uniqKey="Aceves J" first="J." last="Aceves">J. Aceves</name><name sortKey="Liang, J J" sort="Liang, J J" uniqKey="Liang J" first="J. J." last="Liang">J. J. Liang</name><name sortKey="Martinez Fong, D" sort="Martinez Fong, D" uniqKey="Martinez Fong D" first="D." last="Martinez-Fong">D. Martinez-Fong</name><name sortKey="Quirion, R" sort="Quirion, R" uniqKey="Quirion R" first="R." last="Quirion">R. Quirion</name><name sortKey="Sierra, A" sort="Sierra, A" uniqKey="Sierra A" first="A." last="Sierra">A. Sierra</name><name sortKey="Srivastava, L K" sort="Srivastava, L K" uniqKey="Srivastava L" first="L. K." last="Srivastava">L. K. Srivastava</name></country><country name="Canada"><region name="Québec"><name sortKey="Flores, G" sort="Flores, G" uniqKey="Flores G" first="G." last="Flores">G. Flores</name></region><name sortKey="Liang, J J" sort="Liang, J J" uniqKey="Liang J" first="J. J." last="Liang">J. J. Liang</name><name sortKey="Quirion, R" sort="Quirion, R" uniqKey="Quirion R" first="R." last="Quirion">R. Quirion</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Canada/explor/ParkinsonCanadaV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003E10 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 003E10 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Canada
|area= ParkinsonCanadaV1
|flux= Main
|étape= Merge
|type= RBID
|clé= Pascal:99-0278353
|texte= Expression of dopamine receptors in the subthalamic nucleus of the rat : Characterization using reverse transcriptase-polymerase chain reaction and autoradiography
}}
| This area was generated with Dilib version V0.6.29. |  |