Disruption of the autoinhibited state primes the E3 ligase parkin for activation and catalysis.
Identifieur interne : 000761 ( Main/Merge ); précédent : 000760; suivant : 000762Disruption of the autoinhibited state primes the E3 ligase parkin for activation and catalysis.
Auteurs : Atul Kumar [Royaume-Uni] ; Jacob D. Aguirre [Canada] ; Tara E C. Condos [Canada] ; R Julio Martinez-Torres [Royaume-Uni] ; Viduth K. Chaugule [Royaume-Uni] ; Rachel Toth [Royaume-Uni] ; Ramasubramanian Sundaramoorthy [Royaume-Uni] ; Pascal Mercier [Canada] ; Axel Knebel [Royaume-Uni] ; Donald E. Spratt [Canada] ; Kathryn R. Barber [Canada] ; Gary S. Shaw [Canada] ; Helen Walden [Canada]Source :
- The EMBO journal [ 1460-2075 ] ; 2015.
English descriptors
- KwdEn :
- Binding Sites (genetics), Calorimetry, Catalysis, Chromatography, Gel, Crystallization, Enzyme Activation (genetics), Humans, Models, Biological, Models, Molecular, Nuclear Magnetic Resonance, Biomolecular, Phosphorylation, Protein Conformation, Ubiquitin (metabolism), Ubiquitin-Protein Ligases (chemistry), Ubiquitin-Protein Ligases (metabolism).
- MESH :
- chemical , chemistry : Ubiquitin-Protein Ligases.
- chemical , metabolism : Ubiquitin, Ubiquitin-Protein Ligases.
- genetics : Binding Sites, Enzyme Activation.
- Calorimetry, Catalysis, Chromatography, Gel, Crystallization, Humans, Models, Biological, Models, Molecular, Nuclear Magnetic Resonance, Biomolecular, Phosphorylation, Protein Conformation.
Abstract
The PARK2 gene is mutated in 50% of autosomal recessive juvenile parkinsonism (ARJP) cases. It encodes parkin, an E3 ubiquitin ligase of the RBR family. Parkin exists in an autoinhibited state that is activated by phosphorylation of its N-terminal ubiquitin-like (Ubl) domain and binding of phosphoubiquitin. We describe the 1.8 Å crystal structure of human parkin in its fully inhibited state and identify the key interfaces to maintain parkin inhibition. We identify the phosphoubiquitin-binding interface, provide a model for the phosphoubiquitin-parkin complex and show how phosphorylation of the Ubl domain primes parkin for optimal phosphoubiquitin binding. Furthermore, we demonstrate that the addition of phosphoubiquitin leads to displacement of the Ubl domain through loss of structure, unveiling a ubiquitin-binding site used by the E2~Ub conjugate, thus leading to active parkin. We find the role of the Ubl domain is to prevent parkin activity in the absence of the phosphorylation signals, and propose a model for parkin inhibition, optimization for phosphoubiquitin recruitment, release of inhibition by the Ubl domain and engagement with an E2~Ub conjugate. Taken together, this model provides a mechanistic framework for activating parkin.
DOI: 10.15252/embj.201592337
PubMed: 26254304
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Spratt</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Schulich School of Medicine & Dentistry University of Western Ontario, London, ON, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biochemistry, Schulich School of Medicine & Dentistry University of Western Ontario, London, ON</wicri:regionArea><wicri:noRegion>ON</wicri:noRegion></affiliation></author><author><name sortKey="Barber, Kathryn R" sort="Barber, Kathryn R" uniqKey="Barber K" first="Kathryn R" last="Barber">Kathryn R. Barber</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Schulich School of Medicine & Dentistry University of Western Ontario, London, ON, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biochemistry, Schulich School of Medicine & Dentistry University of Western Ontario, London, ON</wicri:regionArea><wicri:noRegion>ON</wicri:noRegion></affiliation></author><author><name sortKey="Shaw, Gary S" sort="Shaw, Gary S" uniqKey="Shaw G" first="Gary S" last="Shaw">Gary S. Shaw</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Schulich School of Medicine & Dentistry University of Western Ontario, London, ON, Canada gshaw1@uwo.ca h.walden@dundee.ac.uk.</nlm:affiliation><country wicri:rule="url">Canada</country><wicri:regionArea>Department of Biochemistry, Schulich School of Medicine & Dentistry University of Western Ontario, London, ON</wicri:regionArea><wicri:noRegion>ON</wicri:noRegion></affiliation></author><author><name sortKey="Walden, Helen" sort="Walden, Helen" uniqKey="Walden H" first="Helen" last="Walden">Helen Walden</name><affiliation wicri:level="1"><nlm:affiliation>MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences University of Dundee, Dundee, UK gshaw1@uwo.ca h.walden@dundee.ac.uk.</nlm:affiliation><country wicri:rule="url">Canada</country><wicri:regionArea>MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences University of Dundee, Dundee</wicri:regionArea><wicri:noRegion>Dundee</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26254304</idno><idno type="pmid">26254304</idno><idno type="doi">10.15252/embj.201592337</idno><idno type="wicri:Area/PubMed/Corpus">000386</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000386</idno><idno type="wicri:Area/PubMed/Curation">000386</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000386</idno><idno type="wicri:Area/PubMed/Checkpoint">000386</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000386</idno><idno type="wicri:Area/Ncbi/Merge">001C71</idno><idno type="wicri:Area/Ncbi/Curation">001C71</idno><idno type="wicri:Area/Ncbi/Checkpoint">001C71</idno><idno type="wicri:Area/Main/Merge">000761</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Disruption of the autoinhibited state primes the E3 ligase parkin for activation and catalysis.</title><author><name sortKey="Kumar, Atul" sort="Kumar, Atul" uniqKey="Kumar A" first="Atul" last="Kumar">Atul Kumar</name><affiliation wicri:level="1"><nlm:affiliation>MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences University of Dundee, Dundee, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences University of Dundee, Dundee</wicri:regionArea><wicri:noRegion>Dundee</wicri:noRegion></affiliation></author><author><name sortKey="Aguirre, Jacob D" sort="Aguirre, Jacob D" uniqKey="Aguirre J" first="Jacob D" last="Aguirre">Jacob D. Aguirre</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Schulich School of Medicine & Dentistry University of Western Ontario, London, ON, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biochemistry, Schulich School of Medicine & Dentistry University of Western Ontario, London, ON</wicri:regionArea><wicri:noRegion>ON</wicri:noRegion></affiliation></author><author><name sortKey="Condos, Tara E C" sort="Condos, Tara E C" uniqKey="Condos T" first="Tara E C" last="Condos">Tara E C. Condos</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Schulich School of Medicine & Dentistry University of Western Ontario, London, ON, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biochemistry, Schulich School of Medicine & Dentistry University of Western Ontario, London, ON</wicri:regionArea><wicri:noRegion>ON</wicri:noRegion></affiliation></author><author><name sortKey="Martinez Torres, R Julio" sort="Martinez Torres, R Julio" uniqKey="Martinez Torres R" first="R Julio" last="Martinez-Torres">R Julio Martinez-Torres</name><affiliation wicri:level="1"><nlm:affiliation>MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences University of Dundee, Dundee, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences University of Dundee, Dundee</wicri:regionArea><wicri:noRegion>Dundee</wicri:noRegion></affiliation></author><author><name sortKey="Chaugule, Viduth K" sort="Chaugule, Viduth K" uniqKey="Chaugule V" first="Viduth K" last="Chaugule">Viduth K. Chaugule</name><affiliation wicri:level="1"><nlm:affiliation>MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences University of Dundee, Dundee, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences University of Dundee, Dundee</wicri:regionArea><wicri:noRegion>Dundee</wicri:noRegion></affiliation></author><author><name sortKey="Toth, Rachel" sort="Toth, Rachel" uniqKey="Toth R" first="Rachel" last="Toth">Rachel Toth</name><affiliation wicri:level="1"><nlm:affiliation>MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences University of Dundee, Dundee, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences University of Dundee, Dundee</wicri:regionArea><wicri:noRegion>Dundee</wicri:noRegion></affiliation></author><author><name sortKey="Sundaramoorthy, Ramasubramanian" sort="Sundaramoorthy, Ramasubramanian" uniqKey="Sundaramoorthy R" first="Ramasubramanian" last="Sundaramoorthy">Ramasubramanian Sundaramoorthy</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Gene Regulation and Expression, College of Life Sciences University of Dundee, Dundee, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Centre for Gene Regulation and Expression, College of Life Sciences University of Dundee, Dundee</wicri:regionArea><wicri:noRegion>Dundee</wicri:noRegion></affiliation></author><author><name sortKey="Mercier, Pascal" sort="Mercier, Pascal" uniqKey="Mercier P" first="Pascal" last="Mercier">Pascal Mercier</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Schulich School of Medicine & Dentistry University of Western Ontario, London, ON, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biochemistry, Schulich School of Medicine & Dentistry University of Western Ontario, London, ON</wicri:regionArea><wicri:noRegion>ON</wicri:noRegion></affiliation></author><author><name sortKey="Knebel, Axel" sort="Knebel, Axel" uniqKey="Knebel A" first="Axel" last="Knebel">Axel Knebel</name><affiliation wicri:level="1"><nlm:affiliation>MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences University of Dundee, Dundee, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences University of Dundee, Dundee</wicri:regionArea><wicri:noRegion>Dundee</wicri:noRegion></affiliation></author><author><name sortKey="Spratt, Donald E" sort="Spratt, Donald E" uniqKey="Spratt D" first="Donald E" last="Spratt">Donald E. Spratt</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Schulich School of Medicine & Dentistry University of Western Ontario, London, ON, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biochemistry, Schulich School of Medicine & Dentistry University of Western Ontario, London, ON</wicri:regionArea><wicri:noRegion>ON</wicri:noRegion></affiliation></author><author><name sortKey="Barber, Kathryn R" sort="Barber, Kathryn R" uniqKey="Barber K" first="Kathryn R" last="Barber">Kathryn R. Barber</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Schulich School of Medicine & Dentistry University of Western Ontario, London, ON, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biochemistry, Schulich School of Medicine & Dentistry University of Western Ontario, London, ON</wicri:regionArea><wicri:noRegion>ON</wicri:noRegion></affiliation></author><author><name sortKey="Shaw, Gary S" sort="Shaw, Gary S" uniqKey="Shaw G" first="Gary S" last="Shaw">Gary S. Shaw</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Schulich School of Medicine & Dentistry University of Western Ontario, London, ON, Canada gshaw1@uwo.ca h.walden@dundee.ac.uk.</nlm:affiliation><country wicri:rule="url">Canada</country><wicri:regionArea>Department of Biochemistry, Schulich School of Medicine & Dentistry University of Western Ontario, London, ON</wicri:regionArea><wicri:noRegion>ON</wicri:noRegion></affiliation></author><author><name sortKey="Walden, Helen" sort="Walden, Helen" uniqKey="Walden H" first="Helen" last="Walden">Helen Walden</name><affiliation wicri:level="1"><nlm:affiliation>MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences University of Dundee, Dundee, UK gshaw1@uwo.ca h.walden@dundee.ac.uk.</nlm:affiliation><country wicri:rule="url">Canada</country><wicri:regionArea>MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences University of Dundee, Dundee</wicri:regionArea><wicri:noRegion>Dundee</wicri:noRegion></affiliation></author></analytic><series><title level="j">The EMBO journal</title><idno type="eISSN">1460-2075</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Binding Sites (genetics)</term><term>Calorimetry</term><term>Catalysis</term><term>Chromatography, Gel</term><term>Crystallization</term><term>Enzyme Activation (genetics)</term><term>Humans</term><term>Models, Biological</term><term>Models, Molecular</term><term>Nuclear Magnetic Resonance, Biomolecular</term><term>Phosphorylation</term><term>Protein Conformation</term><term>Ubiquitin (metabolism)</term><term>Ubiquitin-Protein Ligases (chemistry)</term><term>Ubiquitin-Protein Ligases (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Ubiquitin-Protein Ligases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Ubiquitin</term><term>Ubiquitin-Protein Ligases</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Binding Sites</term><term>Enzyme Activation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Calorimetry</term><term>Catalysis</term><term>Chromatography, Gel</term><term>Crystallization</term><term>Humans</term><term>Models, Biological</term><term>Models, Molecular</term><term>Nuclear Magnetic Resonance, Biomolecular</term><term>Phosphorylation</term><term>Protein Conformation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The PARK2 gene is mutated in 50% of autosomal recessive juvenile parkinsonism (ARJP) cases. It encodes parkin, an E3 ubiquitin ligase of the RBR family. Parkin exists in an autoinhibited state that is activated by phosphorylation of its N-terminal ubiquitin-like (Ubl) domain and binding of phosphoubiquitin. We describe the 1.8 Å crystal structure of human parkin in its fully inhibited state and identify the key interfaces to maintain parkin inhibition. We identify the phosphoubiquitin-binding interface, provide a model for the phosphoubiquitin-parkin complex and show how phosphorylation of the Ubl domain primes parkin for optimal phosphoubiquitin binding. Furthermore, we demonstrate that the addition of phosphoubiquitin leads to displacement of the Ubl domain through loss of structure, unveiling a ubiquitin-binding site used by the E2~Ub conjugate, thus leading to active parkin. We find the role of the Ubl domain is to prevent parkin activity in the absence of the phosphorylation signals, and propose a model for parkin inhibition, optimization for phosphoubiquitin recruitment, release of inhibition by the Ubl domain and engagement with an E2~Ub conjugate. Taken together, this model provides a mechanistic framework for activating parkin.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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