Neuroprotective properties of cultured neural progenitor cells are associated with the production of sonic hedgehog
Identifieur interne : 002B12 ( Main/Exploration ); précédent : 002B11; suivant : 002B13Neuroprotective properties of cultured neural progenitor cells are associated with the production of sonic hedgehog
Auteurs : V. F. Rafuse [Canada] ; P. Soundararajan [Canada] ; C. Leopold [Canada] ; H. A. Robertson [Canada]Source :
- Neuroscience [ 0306-4522 ] ; 2005.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Cellule souche.
English descriptors
- KwdEn :
- Animals, Antimetabolites, Apoptosis, Blotting, Western, Bromodeoxyuridine, Cell Count, Cell Survival (physiology), Cells, Cultured, Chick Embryo, Culture Media, Conditioned, Dopamine (physiology), Dopaminergic neuron, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Epidermal Growth Factor (pharmacology), Female, Hedgehog Proteins, Immunohistochemistry, In vitro, Mice, Mice, Transgenic, Movement Disorders (physiopathology), Movement Disorders (therapy), Neurons (physiology), Neuroprotection, Oxidopamine (toxicity), Parkinson Disease (pathology), Parkinson Disease (physiopathology), Parkinson Disease (therapy), Parkinson disease, Precursor cell, Progenitor cell, Stem Cell Transplantation, Stem Cells (physiology), Stem cell, Sympatholytics (toxicity), Trans-Activators (biosynthesis).
- MESH :
- chemical , biosynthesis : Trans-Activators.
- chemical , pharmacology : Epidermal Growth Factor.
- chemical , physiology : Dopamine.
- chemical , toxicity : Oxidopamine, Sympatholytics.
- chemical : Antimetabolites, Bromodeoxyuridine, Culture Media, Conditioned, Hedgehog Proteins.
- pathology : Parkinson Disease.
- physiology : Cell Survival, Neurons, Stem Cells.
- physiopathology : Movement Disorders, Parkinson Disease.
- therapy : Movement Disorders, Parkinson Disease.
- Animals, Blotting, Western, Cell Count, Cells, Cultured, Chick Embryo, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Female, Immunohistochemistry, Mice, Mice, Transgenic, Stem Cell Transplantation.
Abstract
Numerous studies have shown that abnormal motor behavior improves when neural progenitor cells (NPCs) are transplanted into animal models of neurodegeneration. The mechanisms responsible for this improvement are not fully understood. Indirect anatomical evidence suggests that attention of abnormal motor behavior is attributed, at least in part, to the secretion of trophic factors from the transplanted NPCs. However, there is little direct evidence supporting this hypothesis. Here we show that NPCs isolated from the subventricular zone (SVZ) of neonatal mice are highly teratogenic when transplanted into the neural tube of developing chick embryos and are neuroprotective for fetal dopaminergic neurons in culture because they release sonic hedgehog (Shh). In addition, the neuroprotective properties of NPCs can be exploited to promote better long-term survival of transplanted fetal neurons in an animal model of Parkinson's disease. Thus, cultured NPCs isolated from the SVZ can secrete at least one potent mitogen (Shh) that dramatically affects the fate of neighboring cells. This trait may account for some of the improvement in motor behavior often reported in animal models of neurodegeneration after transplantation of cultured NPCs that were isolated from the SVZ.
Affiliations:
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Le document en format XML
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F." last="Rafuse">V. F. Rafuse</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Anatomy and Neurobiology, Sir Charles Tupper Medical Building, Dalhousie University</s1><s2>Halifax, Nova Scotia, B3H 1X5</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Halifax, Nova Scotia, B3H 1X5</wicri:noRegion></affiliation></author><author><name sortKey="Soundararajan, P" sort="Soundararajan, P" uniqKey="Soundararajan P" first="P." last="Soundararajan">P. Soundararajan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Anatomy and Neurobiology, Sir Charles Tupper Medical Building, Dalhousie University</s1><s2>Halifax, Nova Scotia, B3H 1X5</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Halifax, Nova Scotia, B3H 1X5</wicri:noRegion></affiliation></author><author><name sortKey="Leopold, C" sort="Leopold, C" uniqKey="Leopold C" first="C." last="Leopold">C. Leopold</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Pharmacology, Sir Charles Tupper Medical Building, Dalhousie University</s1><s2>Halifax, Nova Scotia, B3H 1X5</s2><s3>CAN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Halifax, Nova Scotia, B3H 1X5</wicri:noRegion></affiliation></author><author><name sortKey="Robertson, H A" sort="Robertson, H A" uniqKey="Robertson H" first="H. A." last="Robertson">H. A. Robertson</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Pharmacology, Sir Charles Tupper Medical Building, Dalhousie University</s1><s2>Halifax, Nova Scotia, B3H 1X5</s2><s3>CAN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Halifax, Nova Scotia, B3H 1X5</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Neuroscience</title><title level="j" type="abbreviated">Neuroscience</title><idno type="ISSN">0306-4522</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Neuroscience</title><title level="j" type="abbreviated">Neuroscience</title><idno type="ISSN">0306-4522</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antimetabolites</term><term>Apoptosis</term><term>Blotting, Western</term><term>Bromodeoxyuridine</term><term>Cell Count</term><term>Cell Survival (physiology)</term><term>Cells, Cultured</term><term>Chick Embryo</term><term>Culture Media, Conditioned</term><term>Dopamine (physiology)</term><term>Dopaminergic neuron</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Epidermal Growth Factor (pharmacology)</term><term>Female</term><term>Hedgehog Proteins</term><term>Immunohistochemistry</term><term>In vitro</term><term>Mice</term><term>Mice, Transgenic</term><term>Movement Disorders (physiopathology)</term><term>Movement Disorders (therapy)</term><term>Neurons (physiology)</term><term>Neuroprotection</term><term>Oxidopamine (toxicity)</term><term>Parkinson Disease (pathology)</term><term>Parkinson Disease (physiopathology)</term><term>Parkinson Disease (therapy)</term><term>Parkinson disease</term><term>Precursor cell</term><term>Progenitor cell</term><term>Stem Cell Transplantation</term><term>Stem Cells (physiology)</term><term>Stem cell</term><term>Sympatholytics (toxicity)</term><term>Trans-Activators (biosynthesis)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Trans-Activators</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Epidermal Growth Factor</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Dopamine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Oxidopamine</term><term>Sympatholytics</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antimetabolites</term><term>Bromodeoxyuridine</term><term>Culture Media, Conditioned</term><term>Hedgehog Proteins</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Parkinson Disease</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Survival</term><term>Neurons</term><term>Stem Cells</term></keywords><keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Movement Disorders</term><term>Parkinson Disease</term></keywords><keywords scheme="MESH" qualifier="therapy" xml:lang="en"><term>Movement Disorders</term><term>Parkinson Disease</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Cell Count</term><term>Cells, Cultured</term><term>Chick Embryo</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Immunohistochemistry</term><term>Mice</term><term>Mice, Transgenic</term><term>Stem Cell Transplantation</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Neuroprotection</term><term>In vitro</term><term>Cellule précurseur</term><term>Cellule progéniteur</term><term>Cellule souche</term><term>Neurone dopaminergique</term><term>Apoptose</term><term>Parkinson maladie</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Cellule souche</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Numerous studies have shown that abnormal motor behavior improves when neural progenitor cells (NPCs) are transplanted into animal models of neurodegeneration. The mechanisms responsible for this improvement are not fully understood. Indirect anatomical evidence suggests that attention of abnormal motor behavior is attributed, at least in part, to the secretion of trophic factors from the transplanted NPCs. However, there is little direct evidence supporting this hypothesis. Here we show that NPCs isolated from the subventricular zone (SVZ) of neonatal mice are highly teratogenic when transplanted into the neural tube of developing chick embryos and are neuroprotective for fetal dopaminergic neurons in culture because they release sonic hedgehog (Shh). In addition, the neuroprotective properties of NPCs can be exploited to promote better long-term survival of transplanted fetal neurons in an animal model of Parkinson's disease. Thus, cultured NPCs isolated from the SVZ can secrete at least one potent mitogen (Shh) that dramatically affects the fate of neighboring cells. This trait may account for some of the improvement in motor behavior often reported in animal models of neurodegeneration after transplantation of cultured NPCs that were isolated from the SVZ.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Rafuse, V F" sort="Rafuse, V F" uniqKey="Rafuse V" first="V. F." last="Rafuse">V. F. Rafuse</name></noRegion><name sortKey="Leopold, C" sort="Leopold, C" uniqKey="Leopold C" first="C." last="Leopold">C. Leopold</name><name sortKey="Robertson, H A" sort="Robertson, H A" uniqKey="Robertson H" first="H. A." last="Robertson">H. A. Robertson</name><name sortKey="Soundararajan, P" sort="Soundararajan, P" uniqKey="Soundararajan P" first="P." last="Soundararajan">P. Soundararajan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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