Laser Induced Fluorescence Identification of Sinoatrial and Atrioventricular Nodal Conduction Tissue
Identifieur interne : 004396 ( Main/Exploration ); précédent : 004395; suivant : 004397Laser Induced Fluorescence Identification of Sinoatrial and Atrioventricular Nodal Conduction Tissue
Auteurs : Masis Perk [Canada] ; Gregory J. Flynn [Canada] ; Sajad Gulamhusein [Canada] ; Yue Wen [Canada] ; Carolyn Smith [Canada] ; Ben Bathgate [Canada] ; John Tulip [Canada] ; Nollaig A. Parfrey [Canada] ; Alexandra Lucas [Canada]Source :
- Pacing and Clinical Electrophysiology [ 0147-8389 ] ; 1993-08.
English descriptors
Abstract
Transcatheter ahlation of nodal tissue is used for the treatment of arrhythmia resistant to medical therapy. We have investigated the use of laser induced fluorescence spectroscopy for the in vitro recognition of nodal conduction tissue. Twelve fresh human necropsy specimens (< 48 hours)were obtained from sinoatrial node and atrioventricular node areas. Spectra were recorded during excitation at 308 nm (XeCl excimer iaser, 1.5–2.0 mJ/puJse, 10 Hz). Each area examined was marked for subsequent histoiogic examination. Four hundred eleven spectra were obtained, of which 37 contained nodai conduction tissue (21 sinoatrial, 16 atrioventricular node). Normalized fluorescence emission intensity from these areas was compared with that of surrounding endomyocardial tissue at 18 wavelengths and 35 ratios of fluorescence intensity at selected wavelengths. Spectra recorded from nodal tissue could be clearly distinguished hy a visible decrease in fluorescence emission intensity at wavelengths from 440 to 500 nm (P < 0.0006 at 450 nm), peak area, and peak width when compared to that of adjacent atrial endomyocardial tissue. Nodal conduction tissue was also distinguished from ventricular endocardium (14 spectra) by an increase in fluorescence emission at 430 to 550 nm (P < 0.0001). The specificity was 73% and 88% and the sensitivity was 73% and 60% for sinus nodal and atrioventricular nodal conduction tissue identification, respectively. A ratio of fluorescence emission intensity > 1.3 for 380/475 nm was able to detect nodal conducfion tissue (P < 0.001). Conclusion. Laser induced fluorescence can differentiate nodal conduction tissue from atrial and ventricular endocardium and may provide a new diagnostic tool for the recognition and subsequent ablation of nodal conduction tissue.
Url:
DOI: 10.1111/j.1540-8159.1993.tb01041.x
Affiliations:
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We have investigated the use of laser induced fluorescence spectroscopy for the in vitro recognition of nodal conduction tissue. Twelve fresh human necropsy specimens (< 48 hours)were obtained from sinoatrial node and atrioventricular node areas. Spectra were recorded during excitation at 308 nm (XeCl excimer iaser, 1.5–2.0 mJ/puJse, 10 Hz). Each area examined was marked for subsequent histoiogic examination. Four hundred eleven spectra were obtained, of which 37 contained nodai conduction tissue (21 sinoatrial, 16 atrioventricular node). Normalized fluorescence emission intensity from these areas was compared with that of surrounding endomyocardial tissue at 18 wavelengths and 35 ratios of fluorescence intensity at selected wavelengths. Spectra recorded from nodal tissue could be clearly distinguished hy a visible decrease in fluorescence emission intensity at wavelengths from 440 to 500 nm (P < 0.0006 at 450 nm), peak area, and peak width when compared to that of adjacent atrial endomyocardial tissue. Nodal conduction tissue was also distinguished from ventricular endocardium (14 spectra) by an increase in fluorescence emission at 430 to 550 nm (P < 0.0001). The specificity was 73% and 88% and the sensitivity was 73% and 60% for sinus nodal and atrioventricular nodal conduction tissue identification, respectively. A ratio of fluorescence emission intensity > 1.3 for 380/475 nm was able to detect nodal conducfion tissue (P < 0.001). Conclusion. Laser induced fluorescence can differentiate nodal conduction tissue from atrial and ventricular endocardium and may provide a new diagnostic tool for the recognition and subsequent ablation of nodal conduction tissue.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Perk, Masis" sort="Perk, Masis" uniqKey="Perk M" first="Masis" last="Perk">Masis Perk</name></noRegion><name sortKey="Bathgate, Ben" sort="Bathgate, Ben" uniqKey="Bathgate B" first="Ben" last="Bathgate">Ben Bathgate</name><name sortKey="Flynn, Gregory J" sort="Flynn, Gregory J" uniqKey="Flynn G" first="Gregory J." last="Flynn">Gregory J. Flynn</name><name sortKey="Gulamhusein, Sajad" sort="Gulamhusein, Sajad" uniqKey="Gulamhusein S" first="Sajad" last="Gulamhusein">Sajad Gulamhusein</name><name sortKey="Lucas, Alexandra" sort="Lucas, Alexandra" uniqKey="Lucas A" first="Alexandra" last="Lucas">Alexandra Lucas</name><name sortKey="Lucas, Alexandra" sort="Lucas, Alexandra" uniqKey="Lucas A" first="Alexandra" last="Lucas">Alexandra Lucas</name><name sortKey="Parfrey, Nollaig A" sort="Parfrey, Nollaig A" uniqKey="Parfrey N" first="Nollaig A." last="Parfrey">Nollaig A. Parfrey</name><name sortKey="Smith, Carolyn" sort="Smith, Carolyn" uniqKey="Smith C" first="Carolyn" last="Smith">Carolyn Smith</name><name sortKey="Tulip, John" sort="Tulip, John" uniqKey="Tulip J" first="John" last="Tulip">John Tulip</name><name sortKey="Wen, Yue" sort="Wen, Yue" uniqKey="Wen Y" first="Yue" last="Wen">Yue Wen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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