Binding of the anti-human sperm monoclonal antibody HS-11 to bull spermatozoa is correlated with fertility in vitro
Identifieur interne : 003F22 ( Main/Exploration ); précédent : 003F21; suivant : 003F23Binding of the anti-human sperm monoclonal antibody HS-11 to bull spermatozoa is correlated with fertility in vitro
Auteurs : J. D. Ambrose [Canada] ; R. Rajamahendran [Canada] ; K. Sivakumaran [Canada] ; C. Y. G. Lee [Canada]Source :
- [ 0093-691X ]
Abstract
The present study aimed to determine if there is bull to bull variation in the binding of the anti-human sperm monoclonal antibody (MAb) HS-11 to bull spermatozoa, and to investigate if there is any correlation between HS-11 binding to spermatozoa and in vitro fertility of the bulls tested. Semen samples of a single collection (split frozen in 0.5-ml straws) from 8 dairy bulls were used. Swim-up separated motile spermatozoa were incubated in 90-μ1 drops of capacitation medium (TALP+10 μg/ml heparin) at 39 °C, 5% CO2, 95% air. At 0, 2, 4 and 6 h of incubation HS-11 was added (1:1000 final concentration), and the MAb binding was assessed by indirect immunofluorescence assay (IIFA). The HS-11 binding was indicated by a bright green fluorescence of the sperm acrosome region. In vitro-matured, good quality bovine oocytes were randomly allocated to spermatozoa of each bull for in vitro fertilization. Sperm samples of 2 to 3 bulls were used in each trial until 4 replicates per bull were attained for IVF (n ≈ 100 oocytes/bull) and IIFA experiments. Sperm capacitation status was assessed simultaneously using an egg yolk lysophosphatidylcholine- (LC) induced acrosome reaction assay. The binding of HS-11 to spermatozoa was maximum at 4 h of incubation in most ( 6 8) of the bull semen samples. Significant (P < 0.01) differences were observed between bulls in the binding of HS-11 to their spermatozoa (range 22 ± 8 to 52 ± 5%) at 4 h, but not within replicates. Similarly, variations (P < 0.05) in the cleavage rate were also seen (range 22 ± 9 to 58 ± 7%) between bulls. The HS-11 binding and cleavage were significantly correlated (r = 0.43; n = 32; P < 0.05). The highest percentage of spermatozoa underwent acrosome reaction in response to LC treatment at the 4-h incubation period. This and the linear relationship between HS-11 binding and the cleavage rate observed in the present study together strengthen our earlier suggestion that the binding of the monoclonal antibody HS-11 to bull spermatozoa on a time-dependent manner, may indicate capacitation changes. We conclude that 1) between-bull differences exist in HS-11 binding to spermatozoa, and in the cleavage rate, and 2) HS-11 binding to spermatozoa is correlated with fertility, as determined by the cleavage of bovine oocytes matured and fertilized in vitro.
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DOI: 10.1016/0093-691X(94)00035-S
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<record><TEI wicri:istexFullTextTei="no"><teiHeader><fileDesc><titleStmt><title>Binding of the anti-human sperm monoclonal antibody HS-11 to bull spermatozoa is correlated with fertility in vitro</title><author><name sortKey="Ambrose, J D" sort="Ambrose, J D" uniqKey="Ambrose J" first="J. D." last="Ambrose">J. D. Ambrose</name></author><author><name sortKey="Rajamahendran, R" sort="Rajamahendran, R" uniqKey="Rajamahendran R" first="R." last="Rajamahendran">R. Rajamahendran</name></author><author><name sortKey="Sivakumaran, K" sort="Sivakumaran, K" uniqKey="Sivakumaran K" first="K." last="Sivakumaran">K. Sivakumaran</name></author><author><name sortKey="Lee, C Y G" sort="Lee, C Y G" uniqKey="Lee C" first="C. Y. G." last="Lee">C. Y. G. Lee</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:8F382964FB1F81988F38C342959B861FE239373A</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1016/0093-691X(94)00035-S</idno><idno type="url">https://api-v5.istex.fr/document/8F382964FB1F81988F38C342959B861FE239373A/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000B36</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000B36</idno><idno type="wicri:Area/Istex/Curation">000B36</idno><idno type="wicri:Area/Istex/Checkpoint">001896</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001896</idno><idno type="wicri:doubleKey">0093-691X:1995:Ambrose J:binding:of:the</idno><idno type="wicri:Area/Main/Merge">004644</idno><idno type="wicri:Area/Main/Curation">003F22</idno><idno type="wicri:Area/Main/Exploration">003F22</idno></publicationStmt><sourceDesc><biblStruct><analytic><author><name sortKey="Ambrose, J D" sort="Ambrose, J D" uniqKey="Ambrose J" first="J. D." last="Ambrose">J. D. Ambrose</name><affiliation wicri:level="1"><country>Canada</country><wicri:regionArea>Department of Animal Science, The University of British Columbia, Vancouver, BC</wicri:regionArea><wicri:noRegion>BC</wicri:noRegion></affiliation></author><author><name sortKey="Rajamahendran, R" sort="Rajamahendran, R" uniqKey="Rajamahendran R" first="R." last="Rajamahendran">R. Rajamahendran</name><affiliation wicri:level="1"><country>Canada</country><wicri:regionArea>Department of Animal Science, The University of British Columbia, Vancouver, BC</wicri:regionArea><wicri:noRegion>BC</wicri:noRegion></affiliation></author><author><name sortKey="Sivakumaran, K" sort="Sivakumaran, K" uniqKey="Sivakumaran K" first="K." last="Sivakumaran">K. Sivakumaran</name><affiliation wicri:level="1"><country>Canada</country><wicri:regionArea>Department of Animal Science, The University of British Columbia, Vancouver, BC</wicri:regionArea><wicri:noRegion>BC</wicri:noRegion></affiliation></author><author><name sortKey="Lee, C Y G" sort="Lee, C Y G" uniqKey="Lee C" first="C. Y. G." last="Lee">C. Y. G. Lee</name><affiliation wicri:level="1"><country>Canada</country><wicri:regionArea>Department of Obstetrics and Gynecology, The University of British Columbia, Vancouver, BC</wicri:regionArea><wicri:noRegion>BC</wicri:noRegion></affiliation></author></analytic><series><idno type="ISSN">0093-691X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0093-691X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The present study aimed to determine if there is bull to bull variation in the binding of the anti-human sperm monoclonal antibody (MAb) HS-11 to bull spermatozoa, and to investigate if there is any correlation between HS-11 binding to spermatozoa and in vitro fertility of the bulls tested. Semen samples of a single collection (split frozen in 0.5-ml straws) from 8 dairy bulls were used. Swim-up separated motile spermatozoa were incubated in 90-μ1 drops of capacitation medium (TALP+10 μg/ml heparin) at 39 °C, 5% CO2, 95% air. At 0, 2, 4 and 6 h of incubation HS-11 was added (1:1000 final concentration), and the MAb binding was assessed by indirect immunofluorescence assay (IIFA). The HS-11 binding was indicated by a bright green fluorescence of the sperm acrosome region. In vitro-matured, good quality bovine oocytes were randomly allocated to spermatozoa of each bull for in vitro fertilization. Sperm samples of 2 to 3 bulls were used in each trial until 4 replicates per bull were attained for IVF (n ≈ 100 oocytes/bull) and IIFA experiments. Sperm capacitation status was assessed simultaneously using an egg yolk lysophosphatidylcholine- (LC) induced acrosome reaction assay. The binding of HS-11 to spermatozoa was maximum at 4 h of incubation in most ( 6 8) of the bull semen samples. Significant (P < 0.01) differences were observed between bulls in the binding of HS-11 to their spermatozoa (range 22 ± 8 to 52 ± 5%) at 4 h, but not within replicates. Similarly, variations (P < 0.05) in the cleavage rate were also seen (range 22 ± 9 to 58 ± 7%) between bulls. The HS-11 binding and cleavage were significantly correlated (r = 0.43; n = 32; P < 0.05). The highest percentage of spermatozoa underwent acrosome reaction in response to LC treatment at the 4-h incubation period. This and the linear relationship between HS-11 binding and the cleavage rate observed in the present study together strengthen our earlier suggestion that the binding of the monoclonal antibody HS-11 to bull spermatozoa on a time-dependent manner, may indicate capacitation changes. We conclude that 1) between-bull differences exist in HS-11 binding to spermatozoa, and in the cleavage rate, and 2) HS-11 binding to spermatozoa is correlated with fertility, as determined by the cleavage of bovine oocytes matured and fertilized in vitro.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Ambrose, J D" sort="Ambrose, J D" uniqKey="Ambrose J" first="J. D." last="Ambrose">J. D. Ambrose</name></noRegion><name sortKey="Lee, C Y G" sort="Lee, C Y G" uniqKey="Lee C" first="C. Y. G." last="Lee">C. Y. G. Lee</name><name sortKey="Rajamahendran, R" sort="Rajamahendran, R" uniqKey="Rajamahendran R" first="R." last="Rajamahendran">R. Rajamahendran</name><name sortKey="Sivakumaran, K" sort="Sivakumaran, K" uniqKey="Sivakumaran K" first="K." last="Sivakumaran">K. Sivakumaran</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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