Assessment of ionic quenching on soil ATP bioluminescence reaction
Identifieur interne : 003258 ( Main/Exploration ); précédent : 003257; suivant : 003259Assessment of ionic quenching on soil ATP bioluminescence reaction
Auteurs : G. Wen [Japon] ; R. Paul Voroney [Canada] ; J. J Schoenau [Canada] ; T. Yamamoto [Japon] ; J. Chikushi [Japon]Source :
- Soil Biology and Biochemistry [ 0038-0717 ] ; 2001.
English descriptors
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Abstract
Co-extracted ions with ATP from soils may interfere with ATP luciferin–luciferase luminescence reaction when ATP is assayed. The effects were investigated in a typical concentration range of cations and anions potentially extractable in soils. A commercial ATP assay product (Sigma Chemical Co.) was used. Significant quenching is evidenced from a concentration of 0.10mM with Cu2+ and Zn2+, and 1.00mM with Ca2+. The order of quenching at 1.00mM was: Cu2+>Zn2+>Ca2+=Na+=Mg2+, while Mg2+=Mn2+, both Ca2+ and Na+>Mn2+. The quenching was found to be much more severe with selected special heavy metal cations with quenching in the order: Ti3+>Hg2+>Cr3+. Because cation quenching can be alleviated by addition of EDTA, three forms of EDTA (Mg, Na and acid EDTA) were tested for their suitability for the assay. The Mg-EDTA was found superior to the other two. Presence of PO43− at concentrations of 0.01 and 0.05mM, and NO3− at 0.01 and 0.10mM, significantly enhanced ATP light emission (8–13%). However, SO42− at similar concentrations significantly decreased light emission. The quenching by CO32− and Cl− was only observed at high concentrations (3.20mM and up). The order of quenching for the anions at a concentration of 6.4mM was: PO43−>CO32−>SO42−>NO3−>Cl−. Enhanced or depressed light emission induced by ions would produce significant over or underestimation of soil ATP. While addition of Mg-EDTA may alleviate cation quenching, the interference from anions may require the ATP assay standards be prepared in a solution of similar chemical composition to that in soil ATP extracts.
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DOI: 10.1016/S0038-0717(00)00104-8
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Wen</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Arid Land Research Center, Tottori University, 1390 Hamasaka, Tottori 680-0001</wicri:regionArea><wicri:noRegion>Tottori 680-0001</wicri:noRegion></affiliation><affiliation></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Japon</country></affiliation></author><author><name sortKey="Paul Voroney, R" sort="Paul Voroney, R" uniqKey="Paul Voroney R" first="R" last="Paul Voroney">R. Paul Voroney</name><affiliation wicri:level="1"><country>Canada</country><wicri:regionArea>Department of Land Resource Science, University of Guelph, Ont.</wicri:regionArea><wicri:noRegion>Ont.</wicri:noRegion></affiliation></author><author><name sortKey="Schoenau, J J" sort="Schoenau, J J" uniqKey="Schoenau J" first="J. J" last="Schoenau">J. J Schoenau</name><affiliation wicri:level="1"><country>Canada</country><wicri:regionArea>Department of Soil Science, University of Saskatchewan, SASK</wicri:regionArea><wicri:noRegion>SASK</wicri:noRegion></affiliation></author><author><name sortKey="Yamamoto, T" sort="Yamamoto, T" uniqKey="Yamamoto T" first="T" last="Yamamoto">T. Yamamoto</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Arid Land Research Center, Tottori University, 1390 Hamasaka, Tottori 680-0001</wicri:regionArea><wicri:noRegion>Tottori 680-0001</wicri:noRegion></affiliation></author><author><name sortKey="Chikushi, J" sort="Chikushi, J" uniqKey="Chikushi J" first="J" last="Chikushi">J. Chikushi</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Biotron Institute, Kyushu University, Fukuoka 812</wicri:regionArea><orgName type="university">Université de Kyūshū</orgName><placeName><settlement type="city">Fukuoka</settlement><region type="province">Kyūshū</region><region type="prefecture">Préfecture de Fukuoka</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Soil Biology and Biochemistry</title><title level="j" type="abbrev">SBB</title><idno type="ISSN">0038-0717</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001">2001</date><biblScope unit="volume">33</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="1">1</biblScope><biblScope unit="page" to="7">7</biblScope></imprint><idno type="ISSN">0038-0717</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0038-0717</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>ATP</term><term>Anions</term><term>Cations</term><term>Luciferin–luciferase</term><term>Soil extracts</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Co-extracted ions with ATP from soils may interfere with ATP luciferin–luciferase luminescence reaction when ATP is assayed. The effects were investigated in a typical concentration range of cations and anions potentially extractable in soils. A commercial ATP assay product (Sigma Chemical Co.) was used. Significant quenching is evidenced from a concentration of 0.10mM with Cu2+ and Zn2+, and 1.00mM with Ca2+. The order of quenching at 1.00mM was: Cu2+>Zn2+>Ca2+=Na+=Mg2+, while Mg2+=Mn2+, both Ca2+ and Na+>Mn2+. The quenching was found to be much more severe with selected special heavy metal cations with quenching in the order: Ti3+>Hg2+>Cr3+. Because cation quenching can be alleviated by addition of EDTA, three forms of EDTA (Mg, Na and acid EDTA) were tested for their suitability for the assay. The Mg-EDTA was found superior to the other two. Presence of PO43− at concentrations of 0.01 and 0.05mM, and NO3− at 0.01 and 0.10mM, significantly enhanced ATP light emission (8–13%). However, SO42− at similar concentrations significantly decreased light emission. The quenching by CO32− and Cl− was only observed at high concentrations (3.20mM and up). The order of quenching for the anions at a concentration of 6.4mM was: PO43−>CO32−>SO42−>NO3−>Cl−. Enhanced or depressed light emission induced by ions would produce significant over or underestimation of soil ATP. While addition of Mg-EDTA may alleviate cation quenching, the interference from anions may require the ATP assay standards be prepared in a solution of similar chemical composition to that in soil ATP extracts.</div></front></TEI><affiliations><list><country><li>Canada</li><li>Japon</li></country><region><li>Kyūshū</li><li>Préfecture de Fukuoka</li></region><settlement><li>Fukuoka</li></settlement><orgName><li>Université de Kyūshū</li></orgName></list><tree><country name="Japon"><noRegion><name sortKey="Wen, G" sort="Wen, G" uniqKey="Wen G" first="G" last="Wen">G. Wen</name></noRegion><name sortKey="Chikushi, J" sort="Chikushi, J" uniqKey="Chikushi J" first="J" last="Chikushi">J. 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