ParkinsonCanadaV1, Main, Exploration, bibRecord, 002D29

Genetically engineered Sertoli cells are able to survive allogeneic transplantation

Identifieur interne : 002D29 ( Main/Exploration ); précédent : 002D28; suivant : 002D30

Genetically engineered Sertoli cells are able to survive allogeneic transplantation

Auteurs : J. M. Dufour [Canada] ; R. Hemendinger [États-Unis] ; C. R. Halberstadt [États-Unis] ; P. Gores [États-Unis] ; D. F. Emerich [États-Unis] ; G. S. Korbutt [Canada] ; R. V. Rajotte [Canada]

Source :

Gene therapy : (Basingstoke) [ 0969-7128 ] ; 2004.

RBID : Pascal:04-0477633

Descripteurs français

Pascal (Inist)
- Cellule Sertoli, Transplantation, Thérapie génique.

English descriptors

KwdEn :
- Gene therapy, Sertoli cell, Transplantation.

Abstract

The immunoprotective nature of the testis has led to numerous investigations for its ability to protect cellular grafts. Sertoli cells (SCs) are at least partially responsible for this immunoprotective environment and survive allogeneic and xenogeneic transplantation. The ability of SCs to survive transplantation leads to the possibility that they could be engineered to deliver therapeutic proteins. As a model to test this hypothesis, we examined the ability of SCs that produce green fluorescent protein (GFP) to survive transplantation and continue expressing GFP. SCs were isolated from transgenic mice engineered to express GFP and transplanted as aggregates under the kidney capsule of severe combined immunodeficient (SCID) and Balb/c mice. Using this paradigm, it was possible to compare the survival of transgenic SCs directly in both immunodeficient and immunocompetent recipients. Fluorescence microscopy of the kidney capsule and immunohistochemistry of the grafts for GFP and GA TA-4 revealed the presence of GFP-expressing SCs under the kidney capsule of SCID and Balb/c mice at both 30 and 60 days post-transplantation. In contrast, islets transplanted to Balb/c mice were rejected. Thus, SCs survive transplantation and continue to express GFP raising the possibility that SCs can be engineered using transgenic technology to produce proteins, such as insulin, factor VIII, or dopamine for the treatment of diabetes, hemophilia or Parkinson's disease, respectively.

Affiliations:

Canada, États-Unis

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Le document en format XML

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</affiliation>
</author>
<author><name sortKey="Korbutt, G S" sort="Korbutt, G S" uniqKey="Korbutt G" first="G. S." last="Korbutt">G. S. Korbutt</name>
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</affiliation>
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</affiliation>
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<series><title level="j" type="main">Gene therapy : (Basingstoke)</title>
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<front><div type="abstract" xml:lang="en">The immunoprotective nature of the testis has led to numerous investigations for its ability to protect cellular grafts. Sertoli cells (SCs) are at least partially responsible for this immunoprotective environment and survive allogeneic and xenogeneic transplantation. The ability of SCs to survive transplantation leads to the possibility that they could be engineered to deliver therapeutic proteins. As a model to test this hypothesis, we examined the ability of SCs that produce green fluorescent protein (GFP) to survive transplantation and continue expressing GFP. SCs were isolated from transgenic mice engineered to express GFP and transplanted as aggregates under the kidney capsule of severe combined immunodeficient (SCID) and Balb/c mice. Using this paradigm, it was possible to compare the survival of transgenic SCs directly in both immunodeficient and immunocompetent recipients. Fluorescence microscopy of the kidney capsule and immunohistochemistry of the grafts for GFP and GA TA-4 revealed the presence of GFP-expressing SCs under the kidney capsule of SCID and Balb/c mice at both 30 and 60 days post-transplantation. In contrast, islets transplanted to Balb/c mice were rejected. Thus, SCs survive transplantation and continue to express GFP raising the possibility that SCs can be engineered using transgenic technology to produce proteins, such as insulin, factor VIII, or dopamine for the treatment of diabetes, hemophilia or Parkinson's disease, respectively.</div>
</front>
</TEI>
<affiliations><list><country><li>Canada</li>
<li>États-Unis</li>
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<tree><country name="Canada"><noRegion><name sortKey="Dufour, J M" sort="Dufour, J M" uniqKey="Dufour J" first="J. M." last="Dufour">J. M. Dufour</name>
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<name sortKey="Korbutt, G S" sort="Korbutt, G S" uniqKey="Korbutt G" first="G. S." last="Korbutt">G. S. Korbutt</name>
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<name sortKey="Rajotte, R V" sort="Rajotte, R V" uniqKey="Rajotte R" first="R. V." last="Rajotte">R. V. Rajotte</name>
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<name sortKey="Halberstadt, C R" sort="Halberstadt, C R" uniqKey="Halberstadt C" first="C. R." last="Halberstadt">C. R. Halberstadt</name>
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Genetically engineered Sertoli cells are able to survive allogeneic transplantation

Genetically engineered Sertoli cells are able to survive allogeneic transplantation

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri