ParkinsonCanadaV1, Main, Exploration, bibRecord, 001932

NF-κB signaling inhibits ubiquitin carboxyl-terminal hydrolase L1 gene expression

Identifieur interne : 001932 ( Main/Exploration ); précédent : 001931; suivant : 001933

NF-κB signaling inhibits ubiquitin carboxyl-terminal hydrolase L1 gene expression

Auteurs : RUITAO WANG [Canada, République populaire de Chine] ; MINGMING ZHANG [Canada] ; WEIHUI ZHOU [Canada] ; Philip T. T. Ly [Canada] ; FANG CAI [Canada] ; WEIHONG SONG [Canada]

Source :

Journal of neurochemistry [ 0022-3042 ] ; 2011.

RBID : Pascal:11-0160364

Descripteurs français

Pascal (Inist)
- Facteur transcription NFκB, Transduction signal, Ubiquitine, Hydrolases, Expression génique, Protéine, Neurone, Viabilité, Trouble de la mémoire, Modèle animal, Gène, Démence d'Alzheimer, Transcription, Facteur transcription CREB, Homme.
Wicri :
- topic : Homme.

English descriptors

KwdEn :
- Alzheimer disease, Animal model, Gene, Gene expression, Human, Hydrolases, Memory disorder, Neuron, Protein, Signal transduction, Transcription, Transcription factor CREB, Transcription factor NFκB, Ubiquitin, Viability.

Abstract

Ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) is a deubiquitinating enzyme that plays a regulatory role in targeting proteins for proteasomal degradation. UCH-L1 is highly expressed in neurons and has been demonstrated to promote cell viability and maintain neuronal integrity. Reduced UCH-L1 levels have been observed in various neurodegenerative diseases, and expression of UCH-L1 can rescue synaptic dysfunction and memory deficits in Alzheimer's Disease model mice. However, the mechanisms regulating UCH-L1 expression have not been determined. In this study, we cloned a 1782 bp of the 5' flanking region of the human UCH-L1 gene and identified a 43 bp fragment containing the transcription start site as the minimal region necessary for promoter activity. Sequence analysis revealed several putative regulatory elements including NF-κB, NFAT, CREB, NRSF, YY1, AP1, and STAT in the UCH-L1 promoter. A functional NF-κB response element was identified in the UCH-L1 promoter region. Expression of NF-κB suppressed UCH-L1 gene transcription. In the RelA knockout system where NF-κB activity is ablated, UCH-L1 expression was significantly increased. Furthermore, activation of NF-κB signaling by the inflammatory stimulator lipopolysaccharide and TNFα resulted in a decrease of UCH-L1 gene expression by inhibiting its transcription. As NF-κB is an important signaling module in inflammatory response, our study suggests a possibility that inflammation might compromise neuronal functions via the interaction of NF-κB and UCH-L1. A better understanding of the NF-κB-regulated UCH-L1 transcription will provide insights to the role of inflammatory responses in Alzheimer's disease and Parkinson's disease.

Affiliations:

Canada, République populaire de Chine

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000337
to stream PascalFrancis, to step Corpus: 000369
to stream PascalFrancis, to step Curation: 000926
to stream PascalFrancis, to step Checkpoint: 000249
to stream Main, to step Merge: 001A24
to stream Main, to step Curation: 001932

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">NF-κB signaling inhibits ubiquitin carboxyl-terminal hydrolase L1 gene expression</title>
<author><name sortKey="Ruitao Wang" sort="Ruitao Wang" uniqKey="Ruitao Wang" last="Ruitao Wang">RUITAO WANG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Townsend Family Laboratories, Department of Psychiatry, Brain Research Center, Graduate Program in Neuroscience, The University of British Columbia</s1>
<s2>Vancouver, British Columbia</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Vancouver, British Columbia</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Geriatrics, The Second Affiliated Hospital, Harbin Medical University</s1>
<s2>Harbin, Heilongjiang</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Harbin, Heilongjiang</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mingming Zhang" sort="Mingming Zhang" uniqKey="Mingming Zhang" last="Mingming Zhang">MINGMING ZHANG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Townsend Family Laboratories, Department of Psychiatry, Brain Research Center, Graduate Program in Neuroscience, The University of British Columbia</s1>
<s2>Vancouver, British Columbia</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Vancouver, British Columbia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Weihui Zhou" sort="Weihui Zhou" uniqKey="Weihui Zhou" last="Weihui Zhou">WEIHUI ZHOU</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Townsend Family Laboratories, Department of Psychiatry, Brain Research Center, Graduate Program in Neuroscience, The University of British Columbia</s1>
<s2>Vancouver, British Columbia</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Vancouver, British Columbia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ly, Philip T T" sort="Ly, Philip T T" uniqKey="Ly P" first="Philip T. T." last="Ly">Philip T. T. Ly</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Townsend Family Laboratories, Department of Psychiatry, Brain Research Center, Graduate Program in Neuroscience, The University of British Columbia</s1>
<s2>Vancouver, British Columbia</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Vancouver, British Columbia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Fang Cai" sort="Fang Cai" uniqKey="Fang Cai" last="Fang Cai">FANG CAI</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Townsend Family Laboratories, Department of Psychiatry, Brain Research Center, Graduate Program in Neuroscience, The University of British Columbia</s1>
<s2>Vancouver, British Columbia</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Vancouver, British Columbia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Weihong Song" sort="Weihong Song" uniqKey="Weihong Song" last="Weihong Song">WEIHONG SONG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Townsend Family Laboratories, Department of Psychiatry, Brain Research Center, Graduate Program in Neuroscience, The University of British Columbia</s1>
<s2>Vancouver, British Columbia</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Vancouver, British Columbia</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">11-0160364</idno>
<date when="2011">2011</date>
<idno type="stanalyst">PASCAL 11-0160364 INIST</idno>
<idno type="RBID">Pascal:11-0160364</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000337</idno>
<idno type="stanalyst">FRANCIS 11-0160364 INIST</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000369</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000926</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000249</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000249</idno>
<idno type="wicri:doubleKey">0022-3042:2011:Ruitao Wang:nf:b:signaling</idno>
<idno type="wicri:Area/Main/Merge">001A24</idno>
<idno type="wicri:Area/Main/Curation">001932</idno>
<idno type="wicri:Area/Main/Exploration">001932</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">NF-κB signaling inhibits ubiquitin carboxyl-terminal hydrolase L1 gene expression</title>
<author><name sortKey="Ruitao Wang" sort="Ruitao Wang" uniqKey="Ruitao Wang" last="Ruitao Wang">RUITAO WANG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Townsend Family Laboratories, Department of Psychiatry, Brain Research Center, Graduate Program in Neuroscience, The University of British Columbia</s1>
<s2>Vancouver, British Columbia</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Vancouver, British Columbia</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Geriatrics, The Second Affiliated Hospital, Harbin Medical University</s1>
<s2>Harbin, Heilongjiang</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Harbin, Heilongjiang</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mingming Zhang" sort="Mingming Zhang" uniqKey="Mingming Zhang" last="Mingming Zhang">MINGMING ZHANG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Townsend Family Laboratories, Department of Psychiatry, Brain Research Center, Graduate Program in Neuroscience, The University of British Columbia</s1>
<s2>Vancouver, British Columbia</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Vancouver, British Columbia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Weihui Zhou" sort="Weihui Zhou" uniqKey="Weihui Zhou" last="Weihui Zhou">WEIHUI ZHOU</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Townsend Family Laboratories, Department of Psychiatry, Brain Research Center, Graduate Program in Neuroscience, The University of British Columbia</s1>
<s2>Vancouver, British Columbia</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Vancouver, British Columbia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ly, Philip T T" sort="Ly, Philip T T" uniqKey="Ly P" first="Philip T. T." last="Ly">Philip T. T. Ly</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Townsend Family Laboratories, Department of Psychiatry, Brain Research Center, Graduate Program in Neuroscience, The University of British Columbia</s1>
<s2>Vancouver, British Columbia</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Vancouver, British Columbia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Fang Cai" sort="Fang Cai" uniqKey="Fang Cai" last="Fang Cai">FANG CAI</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Townsend Family Laboratories, Department of Psychiatry, Brain Research Center, Graduate Program in Neuroscience, The University of British Columbia</s1>
<s2>Vancouver, British Columbia</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Vancouver, British Columbia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Weihong Song" sort="Weihong Song" uniqKey="Weihong Song" last="Weihong Song">WEIHONG SONG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Townsend Family Laboratories, Department of Psychiatry, Brain Research Center, Graduate Program in Neuroscience, The University of British Columbia</s1>
<s2>Vancouver, British Columbia</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Vancouver, British Columbia</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of neurochemistry</title>
<title level="j" type="abbreviated">J. neurochem.</title>
<idno type="ISSN">0022-3042</idno>
<imprint><date when="2011">2011</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of neurochemistry</title>
<title level="j" type="abbreviated">J. neurochem.</title>
<idno type="ISSN">0022-3042</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alzheimer disease</term>
<term>Animal model</term>
<term>Gene</term>
<term>Gene expression</term>
<term>Human</term>
<term>Hydrolases</term>
<term>Memory disorder</term>
<term>Neuron</term>
<term>Protein</term>
<term>Signal transduction</term>
<term>Transcription</term>
<term>Transcription factor CREB</term>
<term>Transcription factor NFκB</term>
<term>Ubiquitin</term>
<term>Viability</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Facteur transcription NFκB</term>
<term>Transduction signal</term>
<term>Ubiquitine</term>
<term>Hydrolases</term>
<term>Expression génique</term>
<term>Protéine</term>
<term>Neurone</term>
<term>Viabilité</term>
<term>Trouble de la mémoire</term>
<term>Modèle animal</term>
<term>Gène</term>
<term>Démence d'Alzheimer</term>
<term>Transcription</term>
<term>Facteur transcription CREB</term>
<term>Homme</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) is a deubiquitinating enzyme that plays a regulatory role in targeting proteins for proteasomal degradation. UCH-L1 is highly expressed in neurons and has been demonstrated to promote cell viability and maintain neuronal integrity. Reduced UCH-L1 levels have been observed in various neurodegenerative diseases, and expression of UCH-L1 can rescue synaptic dysfunction and memory deficits in Alzheimer's Disease model mice. However, the mechanisms regulating UCH-L1 expression have not been determined. In this study, we cloned a 1782 bp of the 5' flanking region of the human UCH-L1 gene and identified a 43 bp fragment containing the transcription start site as the minimal region necessary for promoter activity. Sequence analysis revealed several putative regulatory elements including NF-κB, NFAT, CREB, NRSF, YY1, AP1, and STAT in the UCH-L1 promoter. A functional NF-κB response element was identified in the UCH-L1 promoter region. Expression of NF-κB suppressed UCH-L1 gene transcription. In the RelA knockout system where NF-κB activity is ablated, UCH-L1 expression was significantly increased. Furthermore, activation of NF-κB signaling by the inflammatory stimulator lipopolysaccharide and TNFα resulted in a decrease of UCH-L1 gene expression by inhibiting its transcription. As NF-κB is an important signaling module in inflammatory response, our study suggests a possibility that inflammation might compromise neuronal functions via the interaction of NF-κB and UCH-L1. A better understanding of the NF-κB-regulated UCH-L1 transcription will provide insights to the role of inflammatory responses in Alzheimer's disease and Parkinson's disease.</div>
</front>
</TEI>
<affiliations><list><country><li>Canada</li>
<li>République populaire de Chine</li>
</country>
</list>
<tree><country name="Canada"><noRegion><name sortKey="Ruitao Wang" sort="Ruitao Wang" uniqKey="Ruitao Wang" last="Ruitao Wang">RUITAO WANG</name>
</noRegion>
<name sortKey="Fang Cai" sort="Fang Cai" uniqKey="Fang Cai" last="Fang Cai">FANG CAI</name>
<name sortKey="Ly, Philip T T" sort="Ly, Philip T T" uniqKey="Ly P" first="Philip T. T." last="Ly">Philip T. T. Ly</name>
<name sortKey="Mingming Zhang" sort="Mingming Zhang" uniqKey="Mingming Zhang" last="Mingming Zhang">MINGMING ZHANG</name>
<name sortKey="Weihong Song" sort="Weihong Song" uniqKey="Weihong Song" last="Weihong Song">WEIHONG SONG</name>
<name sortKey="Weihui Zhou" sort="Weihui Zhou" uniqKey="Weihui Zhou" last="Weihui Zhou">WEIHUI ZHOU</name>
</country>
<country name="République populaire de Chine"><noRegion><name sortKey="Ruitao Wang" sort="Ruitao Wang" uniqKey="Ruitao Wang" last="Ruitao Wang">RUITAO WANG</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Canada/explor/ParkinsonCanadaV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001932 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001932 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Canada
   |area=    ParkinsonCanadaV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     Pascal:11-0160364
   |texte=   NF-κB signaling inhibits ubiquitin carboxyl-terminal hydrolase L1 gene expression
}}

This area was generated with Dilib version V0.6.29.
Data generation: Thu May 4 22:20:19 2017. Site generation: Fri Dec 23 23:17:26 2022

	La maladie de Parkinson au Canada (serveur d'exploration)
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

La maladie de Parkinson au Canada (serveur d'exploration)

NF-κB signaling inhibits ubiquitin carboxyl-terminal hydrolase L1 gene expression

NF-κB signaling inhibits ubiquitin carboxyl-terminal hydrolase L1 gene expression

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri