The diagnosis of human fascioliasis by enzyme-linked immunosorbent assay (ELISA) using recombinant cathepsin L protease.
Identifieur interne : 000C51 ( Main/Exploration ); précédent : 000C50; suivant : 000C52The diagnosis of human fascioliasis by enzyme-linked immunosorbent assay (ELISA) using recombinant cathepsin L protease.
Auteurs : Bibiana Gonzales Santana [Canada] ; John P. Dalton ; Fabio Vasquez Camargo ; Michael Parkinson ; Momar NdaoSource :
- PLoS neglected tropical diseases [ 1935-2735 ] ; 2013.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (blood), Antigens, Helminth (genetics), Cathepsin L (genetics), Clinical Laboratory Techniques (methods), Enzyme-Linked Immunosorbent Assay (methods), Fasciola hepatica (immunology), Fascioliasis (diagnosis), Helminth Proteins (genetics), Humans, Parasitology (methods), Recombinant Proteins (genetics), Sensitivity and Specificity.
- MESH :
- chemical , blood : Antibodies, Helminth.
- chemical , genetics : Antigens, Helminth, Cathepsin L, Helminth Proteins, Recombinant Proteins.
- diagnosis : Fascioliasis.
- immunology : Fasciola hepatica.
- methods : Clinical Laboratory Techniques, Enzyme-Linked Immunosorbent Assay, Parasitology.
- Animals, Humans, Sensitivity and Specificity.
Abstract
Fascioliasis is a worldwide parasitic disease of domestic animals caused by helminths of the genus Fasciola. In many parts of the world, particularly in poor rural areas where animal disease is endemic, the parasite also infects humans. Adult parasites reside in the bile ducts of the host and therefore diagnosis of human fascioliasis is usually achieved by coprological examinations that search for parasite eggs that are carried into the intestine with the bile juices. However, these methods are insensitive due to the fact that eggs are released sporadically and may be missed in low-level infections, and fasciola eggs may be misclassified as other parasites, leading to problems with specificity. Furthermore, acute clinical symptoms as a result of parasites migrating to the bile ducts appear before the parasite matures and begins egg laying. A human immune response to Fasciola antigens occurs early in infection. Therefore, an immunological method such as ELISA may be a more reliable, easy and cheap means to diagnose human fascioliasis than coprological analysis.
DOI: 10.1371/journal.pntd.0002414
PubMed: 24069474
Affiliations:
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Dalton</name></author><author><name sortKey="Vasquez Camargo, Fabio" sort="Vasquez Camargo, Fabio" uniqKey="Vasquez Camargo F" first="Fabio" last="Vasquez Camargo">Fabio Vasquez Camargo</name></author><author><name sortKey="Parkinson, Michael" sort="Parkinson, Michael" uniqKey="Parkinson M" first="Michael" last="Parkinson">Michael Parkinson</name></author><author><name sortKey="Ndao, Momar" sort="Ndao, Momar" uniqKey="Ndao M" first="Momar" last="Ndao">Momar Ndao</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:24069474</idno><idno type="pmid">24069474</idno><idno type="doi">10.1371/journal.pntd.0002414</idno><idno type="wicri:Area/PubMed/Corpus">000866</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000866</idno><idno type="wicri:Area/PubMed/Curation">000866</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000866</idno><idno type="wicri:Area/PubMed/Checkpoint">000866</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000866</idno><idno type="wicri:Area/Ncbi/Merge">001573</idno><idno type="wicri:Area/Ncbi/Curation">001573</idno><idno type="wicri:Area/Ncbi/Checkpoint">001573</idno><idno type="wicri:Area/Main/Merge">000C56</idno><idno type="wicri:Area/Main/Curation">000C51</idno><idno type="wicri:Area/Main/Exploration">000C51</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The diagnosis of human fascioliasis by enzyme-linked immunosorbent assay (ELISA) using recombinant cathepsin L protease.</title><author><name sortKey="Gonzales Santana, Bibiana" sort="Gonzales Santana, Bibiana" uniqKey="Gonzales Santana B" first="Bibiana" last="Gonzales Santana">Bibiana Gonzales Santana</name><affiliation wicri:level="1"><nlm:affiliation>National Reference Center for Parasitology, the Research Institute of the McGill University Health Center, Montreal, Quebec, Canada ; Institute of Parasitology, McGill University, Ste-Anne-de-Bellevue, Quebec, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>National Reference Center for Parasitology, the Research Institute of the McGill University Health Center, Montreal, Quebec, Canada ; Institute of Parasitology, McGill University, Ste-Anne-de-Bellevue, Quebec</wicri:regionArea><wicri:noRegion>Quebec</wicri:noRegion></affiliation></author><author><name sortKey="Dalton, John P" sort="Dalton, John P" uniqKey="Dalton J" first="John P" last="Dalton">John P. Dalton</name></author><author><name sortKey="Vasquez Camargo, Fabio" sort="Vasquez Camargo, Fabio" uniqKey="Vasquez Camargo F" first="Fabio" last="Vasquez Camargo">Fabio Vasquez Camargo</name></author><author><name sortKey="Parkinson, Michael" sort="Parkinson, Michael" uniqKey="Parkinson M" first="Michael" last="Parkinson">Michael Parkinson</name></author><author><name sortKey="Ndao, Momar" sort="Ndao, Momar" uniqKey="Ndao M" first="Momar" last="Ndao">Momar Ndao</name></author></analytic><series><title level="j">PLoS neglected tropical diseases</title><idno type="eISSN">1935-2735</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (blood)</term><term>Antigens, Helminth (genetics)</term><term>Cathepsin L (genetics)</term><term>Clinical Laboratory Techniques (methods)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Fasciola hepatica (immunology)</term><term>Fascioliasis (diagnosis)</term><term>Helminth Proteins (genetics)</term><term>Humans</term><term>Parasitology (methods)</term><term>Recombinant Proteins (genetics)</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Helminth</term><term>Cathepsin L</term><term>Helminth Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Fascioliasis</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Fasciola hepatica</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Clinical Laboratory Techniques</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Parasitology</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Sensitivity and Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Fascioliasis is a worldwide parasitic disease of domestic animals caused by helminths of the genus Fasciola. In many parts of the world, particularly in poor rural areas where animal disease is endemic, the parasite also infects humans. Adult parasites reside in the bile ducts of the host and therefore diagnosis of human fascioliasis is usually achieved by coprological examinations that search for parasite eggs that are carried into the intestine with the bile juices. However, these methods are insensitive due to the fact that eggs are released sporadically and may be missed in low-level infections, and fasciola eggs may be misclassified as other parasites, leading to problems with specificity. Furthermore, acute clinical symptoms as a result of parasites migrating to the bile ducts appear before the parasite matures and begins egg laying. A human immune response to Fasciola antigens occurs early in infection. Therefore, an immunological method such as ELISA may be a more reliable, easy and cheap means to diagnose human fascioliasis than coprological analysis.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Dalton, John P" sort="Dalton, John P" uniqKey="Dalton J" first="John P" last="Dalton">John P. Dalton</name><name sortKey="Ndao, Momar" sort="Ndao, Momar" uniqKey="Ndao M" first="Momar" last="Ndao">Momar Ndao</name><name sortKey="Parkinson, Michael" sort="Parkinson, Michael" uniqKey="Parkinson M" first="Michael" last="Parkinson">Michael Parkinson</name><name sortKey="Vasquez Camargo, Fabio" sort="Vasquez Camargo, Fabio" uniqKey="Vasquez Camargo F" first="Fabio" last="Vasquez Camargo">Fabio Vasquez Camargo</name></noCountry><country name="Canada"><noRegion><name sortKey="Gonzales Santana, Bibiana" sort="Gonzales Santana, Bibiana" uniqKey="Gonzales Santana B" first="Bibiana" last="Gonzales Santana">Bibiana Gonzales Santana</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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