La maladie de Parkinson au Canada (serveur d'exploration)

Attention, ce site est en cours de développement !
Attention, site généré par des moyens informatiques à partir de corpus bruts.
Les informations ne sont donc pas validées.

Expression of a novel gene product by transplants of genetically modified primary fibroblasts in the central nervous system

Identifieur interne : 000F88 ( Istex/Curation ); précédent : 000F87; suivant : 000F89

Expression of a novel gene product by transplants of genetically modified primary fibroblasts in the central nervous system

Auteurs : D. B Doering [Canada] ; P. L. Chang [Canada]

Source :

RBID : ISTEX:07649CF83AC844F12B1A5F04B794E7FC970BE685

English descriptors

Abstract

Primary fibroblasts initiated from skin biopsies of Wistar rats were transfected with a plasmid that encodes the human growth hormone and the neomycin resistance genes. Cell clones selected for G418 resistance and expressing high levels of human growth hormone were propagated in vitro and subsequently transplanted into the cerebral cortex of adult allogeneic rats. Grafts were examined by immunocytochemistry at weekly intervals up to 2 months. Fibroblasts in the transplants survived and expressed growth hormone clearly up to 4 weeks, but showed reduced expression at 6 to 8 weeks. Significant levels of human growth hormone were also detected by radioimmunoassay in the serum of the host rats up to 1 month. The experiments demonstrate that primary fibroblasts can be genetically modified to deliver a new gene product into the central nervous system, and the gene product can pass the blood‐brain barrier to enter the systemic circulation. This model illustrates the potential to introduce desired products into the brain through the genetic alteration of autologous primary fibroblasts.

Url:
DOI: 10.1002/jnr.490290304

Links toward previous steps (curation, corpus...)


Links to Exploration step

ISTEX:07649CF83AC844F12B1A5F04B794E7FC970BE685

Le document en format XML

<record>
<TEI wicri:istexFullTextTei="biblStruct">
<teiHeader>
<fileDesc>
<titleStmt>
<title xml:lang="en">Expression of a novel gene product by transplants of genetically modified primary fibroblasts in the central nervous system</title>
<author>
<name sortKey="Doering, D B" sort="Doering, D B" uniqKey="Doering D" first="D. B" last="Doering">D. B Doering</name>
<affiliation wicri:level="1">
<mods:affiliation>Department of Biomedical Sciences(Anatomy), McMaster University, Hamilton, Ontario, Canada</mods:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Biomedical Sciences(Anatomy), McMaster University, Hamilton, Ontario</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1">
<mods:affiliation>Division of Anatomy, HSC 1R1, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada L8N 3Z5</mods:affiliation>
<country>Canada</country>
<wicri:regionArea>Division of Anatomy, HSC 1R1, McMaster University, 1200 Main Street West, Hamilton, Ontario</wicri:regionArea>
</affiliation>
</author>
<author>
<name sortKey="Chang, P L" sort="Chang, P L" uniqKey="Chang P" first="P. L." last="Chang">P. L. Chang</name>
<affiliation wicri:level="1">
<mods:affiliation>Department of Biomedical Sciences(Anatomy), McMaster University, Hamilton, Ontario, Canada</mods:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Biomedical Sciences(Anatomy), McMaster University, Hamilton, Ontario</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1">
<mods:affiliation>Department of Pediatrics, McMaster University, Hamilton, Ontario, Canada</mods:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Pediatrics, McMaster University, Hamilton, Ontario</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt>
<idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:07649CF83AC844F12B1A5F04B794E7FC970BE685</idno>
<date when="1991" year="1991">1991</date>
<idno type="doi">10.1002/jnr.490290304</idno>
<idno type="url">https://api-v5.istex.fr/document/07649CF83AC844F12B1A5F04B794E7FC970BE685/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000F88</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000F88</idno>
<idno type="wicri:Area/Istex/Curation">000F88</idno>
</publicationStmt>
<sourceDesc>
<biblStruct>
<analytic>
<title level="a" type="main" xml:lang="en">Expression of a novel gene product by transplants of genetically modified primary fibroblasts in the central nervous system</title>
<author>
<name sortKey="Doering, D B" sort="Doering, D B" uniqKey="Doering D" first="D. B" last="Doering">D. B Doering</name>
<affiliation wicri:level="1">
<mods:affiliation>Department of Biomedical Sciences(Anatomy), McMaster University, Hamilton, Ontario, Canada</mods:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Biomedical Sciences(Anatomy), McMaster University, Hamilton, Ontario</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1">
<mods:affiliation>Division of Anatomy, HSC 1R1, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada L8N 3Z5</mods:affiliation>
<country>Canada</country>
<wicri:regionArea>Division of Anatomy, HSC 1R1, McMaster University, 1200 Main Street West, Hamilton, Ontario</wicri:regionArea>
</affiliation>
</author>
<author>
<name sortKey="Chang, P L" sort="Chang, P L" uniqKey="Chang P" first="P. L." last="Chang">P. L. Chang</name>
<affiliation wicri:level="1">
<mods:affiliation>Department of Biomedical Sciences(Anatomy), McMaster University, Hamilton, Ontario, Canada</mods:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Biomedical Sciences(Anatomy), McMaster University, Hamilton, Ontario</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1">
<mods:affiliation>Department of Pediatrics, McMaster University, Hamilton, Ontario, Canada</mods:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Pediatrics, McMaster University, Hamilton, Ontario</wicri:regionArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series>
<title level="j">Journal of Neuroscience Research</title>
<title level="j" type="abbrev">J. Neurosci. Res.</title>
<idno type="ISSN">0360-4012</idno>
<idno type="eISSN">1097-4547</idno>
<imprint>
<publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher>
<pubPlace>Hoboken</pubPlace>
<date type="published" when="1991-07">1991-07</date>
<biblScope unit="volume">29</biblScope>
<biblScope unit="issue">3</biblScope>
<biblScope unit="page" from="292">292</biblScope>
<biblScope unit="page" to="298">298</biblScope>
</imprint>
<idno type="ISSN">0360-4012</idno>
</series>
<idno type="istex">07649CF83AC844F12B1A5F04B794E7FC970BE685</idno>
<idno type="DOI">10.1002/jnr.490290304</idno>
<idno type="ArticleID">JNR490290304</idno>
</biblStruct>
</sourceDesc>
<seriesStmt>
<idno type="ISSN">0360-4012</idno>
</seriesStmt>
</fileDesc>
<profileDesc>
<textClass>
<keywords scheme="KwdEn" xml:lang="en">
<term>human growth hormone</term>
<term>immunocytochemistry</term>
<term>rat</term>
<term>transfection</term>
</keywords>
</textClass>
<langUsage>
<language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front>
<div type="abstract" xml:lang="en">Primary fibroblasts initiated from skin biopsies of Wistar rats were transfected with a plasmid that encodes the human growth hormone and the neomycin resistance genes. Cell clones selected for G418 resistance and expressing high levels of human growth hormone were propagated in vitro and subsequently transplanted into the cerebral cortex of adult allogeneic rats. Grafts were examined by immunocytochemistry at weekly intervals up to 2 months. Fibroblasts in the transplants survived and expressed growth hormone clearly up to 4 weeks, but showed reduced expression at 6 to 8 weeks. Significant levels of human growth hormone were also detected by radioimmunoassay in the serum of the host rats up to 1 month. The experiments demonstrate that primary fibroblasts can be genetically modified to deliver a new gene product into the central nervous system, and the gene product can pass the blood‐brain barrier to enter the systemic circulation. This model illustrates the potential to introduce desired products into the brain through the genetic alteration of autologous primary fibroblasts.</div>
</front>
</TEI>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Canada/explor/ParkinsonCanadaV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000F88 | SxmlIndent | more

Ou

HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 000F88 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Canada
   |area=    ParkinsonCanadaV1
   |flux=    Istex
   |étape=   Curation
   |type=    RBID
   |clé=     ISTEX:07649CF83AC844F12B1A5F04B794E7FC970BE685
   |texte=   Expression of a novel gene product by transplants of genetically modified primary fibroblasts in the central nervous system
}}

Wicri

This area was generated with Dilib version V0.6.29.
Data generation: Thu May 4 22:20:19 2017. Site generation: Fri Dec 23 23:17:26 2022