Visible labeling of proteins for polyacrylamide gel electrophoresis with dabsyl chloride
Identifieur interne : 000737 ( Istex/Curation ); précédent : 000736; suivant : 000738Visible labeling of proteins for polyacrylamide gel electrophoresis with dabsyl chloride
Auteurs : David Parkinson [Canada] ; James D. Redshaw [Canada]Source :
- Analytical Biochemistry [ 0003-2697 ] ; 1984.
English descriptors
Abstract
Several proteins, which are used as molecular weight markers in polyacrylamide gel electrophoresis, were reacted with dabsyl chloride. This labeled them deep orange and the chromophore attachment was stable throughout the electrophoretic procedure and fixation. Small amounts (10–50 μg) of the labeled proteins could be loaded onto gels and seen with the unaided eye so that the separation during electrophoresis could be followed. Dabsylation did not affect the mobility of the proteins. The location of the orange band gave a good indication of the position of the protein in the gel so that molecular weight estimations could be made during and immediately following electrophoresis.
Url:
DOI: 10.1016/0003-2697(84)90434-2
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Redshaw</name><affiliation><mods:affiliation>To whom correspondence should be addressed.</mods:affiliation><wicri:noCountry code="no comma">To whom correspondence should be addressed.</wicri:noCountry></affiliation><affiliation wicri:level="1"><mods:affiliation>Department of Physiology, Faculty of Medicine, University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta T2N 4N1, Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Physiology, Faculty of Medicine, University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta T2N 4N1</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:C8C6B59DE3A2F6AD1D1DC132E530212DE451B874</idno><date when="1984" year="1984">1984</date><idno type="doi">10.1016/0003-2697(84)90434-2</idno><idno type="url">https://api-v5.istex.fr/document/C8C6B59DE3A2F6AD1D1DC132E530212DE451B874/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000737</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000737</idno><idno type="wicri:Area/Istex/Curation">000737</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Visible labeling of proteins for polyacrylamide gel electrophoresis with dabsyl chloride</title><author><name sortKey="Parkinson, David" sort="Parkinson, David" uniqKey="Parkinson D" first="David" last="Parkinson">David Parkinson</name><affiliation wicri:level="1"><mods:affiliation>Department of Anatomy, Faculty of Medicine, University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta T2N 4N1, Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Anatomy, Faculty of Medicine, University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta T2N 4N1</wicri:regionArea></affiliation></author><author><name sortKey="Redshaw, James D" sort="Redshaw, James D" uniqKey="Redshaw J" first="James D." last="Redshaw">James D. Redshaw</name><affiliation><mods:affiliation>To whom correspondence should be addressed.</mods:affiliation></affiliation><affiliation wicri:level="1"><mods:affiliation>Department of Physiology, Faculty of Medicine, University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta T2N 4N1, Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Physiology, Faculty of Medicine, University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta T2N 4N1</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Analytical Biochemistry</title><title level="j" type="abbrev">YABIO</title><idno type="ISSN">0003-2697</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1984">1984</date><biblScope unit="volume">141</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="121">121</biblScope><biblScope unit="page" to="126">126</biblScope></imprint><idno type="ISSN">0003-2697</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0003-2697</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>dabsyl chloride</term><term>gel electrophoresis</term><term>molecular weight standards</term><term>proteins</term><term>visible</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Several proteins, which are used as molecular weight markers in polyacrylamide gel electrophoresis, were reacted with dabsyl chloride. This labeled them deep orange and the chromophore attachment was stable throughout the electrophoretic procedure and fixation. Small amounts (10–50 μg) of the labeled proteins could be loaded onto gels and seen with the unaided eye so that the separation during electrophoresis could be followed. Dabsylation did not affect the mobility of the proteins. The location of the orange band gave a good indication of the position of the protein in the gel so that molecular weight estimations could be made during and immediately following electrophoresis.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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