Polynucleotide cross-linking by aluminum
Identifieur interne : 002534 ( Istex/Corpus ); précédent : 002533; suivant : 002535Polynucleotide cross-linking by aluminum
Auteurs : S. J. Karlik ; G. L. EichhornSource :
- Journal of Inorganic Biochemistry [ 0162-0134 ] ; 1989.
Abstract
The observations that there was an increased concentration of Al in the brains of Alzheimer's, Guam-Parkinson, and amyotrophic lateral sclerosis disease patients and that there was an apparent localization of the Al in chromatin led to a study of the interaction of Al(III) with DNA. We have previously shown that Al cross-links calf thymus DNA at low pH (S. J. Karlik, G. L. Eichhorn, P. N. Lewis, and D. R. Crapper, Biochemistry 19, 5991 [1980]). Extended studies indicate that cross-linking occurs in DNAs of all base ratios, including polydAdT and polydGdC. Since Al cross-links prevent renaturation in polydAdT, the decrease in the amount of polymer renatured in the presence of Al becomes a quantitative appraisal of the extent of cross-linking. Saturation of cross-linking occurs at a 0.4 ratio of Al to nucleotide phosphate, indicating that potentially 80% of the base pairs are Al bound. Cross-links are broken at elevated pH and by EDTA.
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DOI: 10.1016/0162-0134(89)85001-9
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Eichhorn</name><affiliation><mods:affiliation>Laboratory, of Cellular and Molecular Biology, National Institute on Aging, National Institutes of Health, Gerontology Research Center, Baltimore, Maryland USA</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:294F929010CB1FD08E874569D9596D9DED2822A9</idno><date when="1989" year="1989">1989</date><idno type="doi">10.1016/0162-0134(89)85001-9</idno><idno type="url">https://api-v5.istex.fr/document/294F929010CB1FD08E874569D9596D9DED2822A9/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">002534</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002534</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Polynucleotide cross-linking by aluminum</title><author><name sortKey="Karlik, S J" sort="Karlik, S J" uniqKey="Karlik S" first="S. J." last="Karlik">S. J. Karlik</name><affiliation><mods:affiliation>Department of Diagnostic Radiology, University Hospital, University of Western Ontario, London, Ontario, Canada.</mods:affiliation></affiliation></author><author><name sortKey="Eichhorn, G L" sort="Eichhorn, G L" uniqKey="Eichhorn G" first="G. L." last="Eichhorn">G. L. Eichhorn</name><affiliation><mods:affiliation>Laboratory, of Cellular and Molecular Biology, National Institute on Aging, National Institutes of Health, Gerontology Research Center, Baltimore, Maryland USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Inorganic Biochemistry</title><title level="j" type="abbrev">JIB</title><idno type="ISSN">0162-0134</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1989">1989</date><biblScope unit="volume">37</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="259">259</biblScope><biblScope unit="page" to="269">269</biblScope></imprint><idno type="ISSN">0162-0134</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0162-0134</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The observations that there was an increased concentration of Al in the brains of Alzheimer's, Guam-Parkinson, and amyotrophic lateral sclerosis disease patients and that there was an apparent localization of the Al in chromatin led to a study of the interaction of Al(III) with DNA. We have previously shown that Al cross-links calf thymus DNA at low pH (S. J. Karlik, G. L. Eichhorn, P. N. Lewis, and D. R. Crapper, Biochemistry 19, 5991 [1980]). Extended studies indicate that cross-linking occurs in DNAs of all base ratios, including polydAdT and polydGdC. Since Al cross-links prevent renaturation in polydAdT, the decrease in the amount of polymer renatured in the presence of Al becomes a quantitative appraisal of the extent of cross-linking. Saturation of cross-linking occurs at a 0.4 ratio of Al to nucleotide phosphate, indicating that potentially 80% of the base pairs are Al bound. Cross-links are broken at elevated pH and by EDTA.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>S.J. Karlik</name><affiliations><json:string>Department of Diagnostic Radiology, University Hospital, University of Western Ontario, London, Ontario, Canada.</json:string></affiliations></json:item><json:item><name>G.L. 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Since Al cross-links prevent renaturation in polydAdT, the decrease in the amount of polymer renatured in the presence of Al becomes a quantitative appraisal of the extent of cross-linking. Saturation of cross-linking occurs at a 0.4 ratio of Al to nucleotide phosphate, indicating that potentially 80% of the base pairs are Al bound. 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J. Karlik, Department of Diagnostic Radiology, University Hospital, University of Western Ontario, PO Box 5339, Postal Station A, London, Ontario, Canada N6A SAS.</note><affiliation>Dr. S. J. Karlik, Department of Diagnostic Radiology, University Hospital, University of Western Ontario, PO Box 5339, Postal Station A, London, Ontario, Canada N6A SAS.</affiliation><affiliation>Department of Diagnostic Radiology, University Hospital, University of Western Ontario, London, Ontario, Canada.</affiliation></author><author xml:id="author-0001"><persName><forename type="first">G.L.</forename><surname>Eichhorn</surname></persName><affiliation>Laboratory, of Cellular and Molecular Biology, National Institute on Aging, National Institutes of Health, Gerontology Research Center, Baltimore, Maryland USA</affiliation></author><idno type="istex">294F929010CB1FD08E874569D9596D9DED2822A9</idno><idno type="DOI">10.1016/0162-0134(89)85001-9</idno><idno type="PII">0162-0134(89)85001-9</idno></analytic><monogr><title level="j">Journal of Inorganic Biochemistry</title><title level="j" type="abbrev">JIB</title><idno type="pISSN">0162-0134</idno><idno type="PII">S0162-0134(00)X0260-7</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1989"></date><biblScope unit="volume">37</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="259">259</biblScope><biblScope unit="page" to="269">269</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1989</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The observations that there was an increased concentration of Al in the brains of Alzheimer's, Guam-Parkinson, and amyotrophic lateral sclerosis disease patients and that there was an apparent localization of the Al in chromatin led to a study of the interaction of Al(III) with DNA. We have previously shown that Al cross-links calf thymus DNA at low pH (S. J. Karlik, G. L. Eichhorn, P. N. Lewis, and D. R. Crapper, Biochemistry 19, 5991 [1980]). Extended studies indicate that cross-linking occurs in DNAs of all base ratios, including polydAdT and polydGdC. Since Al cross-links prevent renaturation in polydAdT, the decrease in the amount of polymer renatured in the presence of Al becomes a quantitative appraisal of the extent of cross-linking. Saturation of cross-linking occurs at a 0.4 ratio of Al to nucleotide phosphate, indicating that potentially 80% of the base pairs are Al bound. 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Karlik, Department of Diagnostic Radiology, University Hospital, University of Western Ontario, PO Box 5339, Postal Station A, London, Ontario, Canada N6A SAS.</ce:text></ce:correspondence></ce:author-group><ce:author-group><ce:author><ce:given-name>G.L.</ce:given-name><ce:surname>Eichhorn</ce:surname></ce:author><ce:affiliation><ce:textfn>Laboratory, of Cellular and Molecular Biology, National Institute on Aging, National Institutes of Health, Gerontology Research Center, Baltimore, Maryland USA</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="9" month="2" year="1989"></ce:date-received><ce:date-accepted day="5" month="4" year="1989"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The observations that there was an increased concentration of Al in the brains of Alzheimer's, Guam-Parkinson, and amyotrophic lateral sclerosis disease patients and that there was an apparent localization of the Al in chromatin led to a study of the interaction of Al(III) with DNA. We have previously shown that Al cross-links calf thymus DNA at low pH (S. J. Karlik, G. L. Eichhorn, P. N. Lewis, and D. R. Crapper, Biochemistry 19, 5991 [1980]). Extended studies indicate that cross-linking occurs in DNAs of all base ratios, including polydAdT and polydGdC. Since Al cross-links prevent renaturation in polydAdT, the decrease in the amount of polymer renatured in the presence of Al becomes a quantitative appraisal of the extent of cross-linking. Saturation of cross-linking occurs at a 0.4 ratio of Al to nucleotide phosphate, indicating that potentially 80% of the base pairs are Al bound. 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Karlik, Department of Diagnostic Radiology, University Hospital, University of Western Ontario, PO Box 5339, Postal Station A, London, Ontario, Canada N6A SAS.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.L.</namePart><namePart type="family">Eichhorn</namePart><affiliation>Laboratory, of Cellular and Molecular Biology, National Institute on Aging, National Institutes of Health, Gerontology Research Center, Baltimore, Maryland USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1989</dateIssued><copyrightDate encoding="w3cdtf">1989</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The observations that there was an increased concentration of Al in the brains of Alzheimer's, Guam-Parkinson, and amyotrophic lateral sclerosis disease patients and that there was an apparent localization of the Al in chromatin led to a study of the interaction of Al(III) with DNA. We have previously shown that Al cross-links calf thymus DNA at low pH (S. J. Karlik, G. L. Eichhorn, P. N. Lewis, and D. R. Crapper, Biochemistry 19, 5991 [1980]). Extended studies indicate that cross-linking occurs in DNAs of all base ratios, including polydAdT and polydGdC. Since Al cross-links prevent renaturation in polydAdT, the decrease in the amount of polymer renatured in the presence of Al becomes a quantitative appraisal of the extent of cross-linking. Saturation of cross-linking occurs at a 0.4 ratio of Al to nucleotide phosphate, indicating that potentially 80% of the base pairs are Al bound. Cross-links are broken at elevated pH and by EDTA.</abstract><relatedItem type="host"><titleInfo><title>Journal of Inorganic Biochemistry</title></titleInfo><titleInfo type="abbreviated"><title>JIB</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">1989</dateIssued></originInfo><identifier type="ISSN">0162-0134</identifier><identifier type="PII">S0162-0134(00)X0260-7</identifier><part><date>1989</date><detail type="volume"><number>37</number><caption>vol.</caption></detail><detail type="issue"><number>4</number><caption>no.</caption></detail><extent unit="issue pages"><start>259</start><end>344</end></extent><extent unit="pages"><start>259</start><end>269</end></extent></part></relatedItem><identifier type="istex">294F929010CB1FD08E874569D9596D9DED2822A9</identifier><identifier type="DOI">10.1016/0162-0134(89)85001-9</identifier><identifier type="PII">0162-0134(89)85001-9</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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