Soil nitrogen status as a regulator of carbon substrate flows through microbial communities with elevated CO2
Identifieur interne : 002363 ( Istex/Corpus ); précédent : 002362; suivant : 002364Soil nitrogen status as a regulator of carbon substrate flows through microbial communities with elevated CO2
Auteurs : Susan E. Ziegler ; Sharon A. BillingsSource :
- Journal of Geophysical Research: Biogeosciences [ 0148-0227 ] ; 2011-03.
Abstract
To assess how microbial processing of organic C inputs to forest soils may be influenced by elevated CO2 and altered N dynamics, we followed the fate of 13C‐labeled substrates in soils from the Duke Free Air Carbon Enrichment site where differences in soil N status have been imposed by 7 years of N amendments. Heterotrophic respiration and δ13C of respired CO2‐C and phospholipid fatty acids (PLFA) were measured to track activities of microbial groups and estimate a relative measure of substrate use efficiency (PLFA‐based SUE). Results indicate an increased proportion of fungal and actinomycete activity in elevated CO2 soils, which varied with substrate. The negative effect of N on vanillin phenolic‐C incorporation into actinomycete PLFA suggests legacies of fertilization can mitigate increased C flow into actinomycetes with elevated CO2. Further, the fourfold increase in PLFA‐based SUE for vanillin phenolic‐C in elevated CO2 soils that received N suggests future enhanced N limitation in elevated CO2 soils may promote enhanced respiratory loss relative to incorporation of some C‐substrates into microbial biomass. These short‐term incubations did not reveal greater loss of soil organic carbon via respiration or shifts in SUE with elevated CO2. However, observed relative increases in activity of actinomycetes and fungi with elevated CO2 and mitigation of this effect on actinomycetes with N amendments suggests that elevated CO2 and predicted N limitation may alter the fate of slow‐turnover soil organic matter (SOM) in two competing ways. Investigations need to focus on how these microorganisms may increase slow‐turnover substrate use while possibly enhancing the prevalence of microbial cell wall structures that can serve as precursors of stabilized SOM.
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DOI: 10.1029/2010JG001434
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Soil nitrogen status as a regulator of carbon substrate flows through microbial communities with elevated CO2</title><author><name sortKey="Ziegler, Susan E" sort="Ziegler, Susan E" uniqKey="Ziegler S" first="Susan E." last="Ziegler">Susan E. Ziegler</name><affiliation><mods:affiliation>Department of Earth Science, Memorial University of Newfoundland, St. John's, Newfoundland and Labrador, Canada</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: sziegler@mun.ca</mods:affiliation></affiliation></author><author><name sortKey="Billings, Sharon A" sort="Billings, Sharon A" uniqKey="Billings S" first="Sharon A." last="Billings">Sharon A. 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Ziegler</name><affiliation><mods:affiliation>Department of Earth Science, Memorial University of Newfoundland, St. John's, Newfoundland and Labrador, Canada</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: sziegler@mun.ca</mods:affiliation></affiliation></author><author><name sortKey="Billings, Sharon A" sort="Billings, Sharon A" uniqKey="Billings S" first="Sharon A." last="Billings">Sharon A. Billings</name><affiliation><mods:affiliation>Department of Ecology and Evolutionary Biology and Kansas Biological Survey, University of Kansas, Kansas, Lawrence, USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Geophysical Research: Biogeosciences</title><title level="j" type="abbrev">J. Geophys. Res.</title><idno type="ISSN">0148-0227</idno><idno type="eISSN">2156-2202</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><date type="published" when="2011-03">2011-03</date><biblScope unit="volume">116</biblScope><biblScope unit="issue">G1</biblScope><biblScope unit="page" from="/">n/a</biblScope><biblScope unit="page" to="/">n/a</biblScope></imprint><idno type="ISSN">0148-0227</idno></series><idno type="istex">76992B6B96AC9A9314A96D5789519562A4E97ED5</idno><idno type="DOI">10.1029/2010JG001434</idno><idno type="ArticleID">2010JG001434</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0148-0227</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">To assess how microbial processing of organic C inputs to forest soils may be influenced by elevated CO2 and altered N dynamics, we followed the fate of 13C‐labeled substrates in soils from the Duke Free Air Carbon Enrichment site where differences in soil N status have been imposed by 7 years of N amendments. Heterotrophic respiration and δ13C of respired CO2‐C and phospholipid fatty acids (PLFA) were measured to track activities of microbial groups and estimate a relative measure of substrate use efficiency (PLFA‐based SUE). Results indicate an increased proportion of fungal and actinomycete activity in elevated CO2 soils, which varied with substrate. The negative effect of N on vanillin phenolic‐C incorporation into actinomycete PLFA suggests legacies of fertilization can mitigate increased C flow into actinomycetes with elevated CO2. Further, the fourfold increase in PLFA‐based SUE for vanillin phenolic‐C in elevated CO2 soils that received N suggests future enhanced N limitation in elevated CO2 soils may promote enhanced respiratory loss relative to incorporation of some C‐substrates into microbial biomass. These short‐term incubations did not reveal greater loss of soil organic carbon via respiration or shifts in SUE with elevated CO2. However, observed relative increases in activity of actinomycetes and fungi with elevated CO2 and mitigation of this effect on actinomycetes with N amendments suggests that elevated CO2 and predicted N limitation may alter the fate of slow‐turnover soil organic matter (SOM) in two competing ways. Investigations need to focus on how these microorganisms may increase slow‐turnover substrate use while possibly enhancing the prevalence of microbial cell wall structures that can serve as precursors of stabilized SOM.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Susan E. Ziegler</name><affiliations><json:string>Department of Earth Science, Memorial University of Newfoundland, St. John's, Newfoundland and Labrador, Canada</json:string><json:string>E-mail: sziegler@mun.ca</json:string></affiliations></json:item><json:item><name>Sharon A. Billings</name><affiliations><json:string>Department of Ecology and Evolutionary Biology and Kansas Biological Survey, University of Kansas, Kansas, Lawrence, USA</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>soil organic carbon</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>heterotrophic respiration</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>elevated CO2</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>progressive N limitation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>phospholipid fatty acids</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>stable carbon isotopes</value></json:item></subject><articleId><json:string>2010JG001434</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>To assess how microbial processing of organic C inputs to forest soils may be influenced by elevated CO2 and altered N dynamics, we followed the fate of 13C‐labeled substrates in soils from the Duke Free Air Carbon Enrichment site where differences in soil N status have been imposed by 7 years of N amendments. Heterotrophic respiration and δ13C of respired CO2‐C and phospholipid fatty acids (PLFA) were measured to track activities of microbial groups and estimate a relative measure of substrate use efficiency (PLFA‐based SUE). Results indicate an increased proportion of fungal and actinomycete activity in elevated CO2 soils, which varied with substrate. The negative effect of N on vanillin phenolic‐C incorporation into actinomycete PLFA suggests legacies of fertilization can mitigate increased C flow into actinomycetes with elevated CO2. Further, the fourfold increase in PLFA‐based SUE for vanillin phenolic‐C in elevated CO2 soils that received N suggests future enhanced N limitation in elevated CO2 soils may promote enhanced respiratory loss relative to incorporation of some C‐substrates into microbial biomass. These short‐term incubations did not reveal greater loss of soil organic carbon via respiration or shifts in SUE with elevated CO2. However, observed relative increases in activity of actinomycetes and fungi with elevated CO2 and mitigation of this effect on actinomycetes with N amendments suggests that elevated CO2 and predicted N limitation may alter the fate of slow‐turnover soil organic matter (SOM) in two competing ways. 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Heterotrophic respiration and δ13C of respired CO2‐C and phospholipid fatty acids (PLFA) were measured to track activities of microbial groups and estimate a relative measure of substrate use efficiency (PLFA‐based SUE). Results indicate an increased proportion of fungal and actinomycete activity in elevated CO2 soils, which varied with substrate. The negative effect of N on vanillin phenolic‐C incorporation into actinomycete PLFA suggests legacies of fertilization can mitigate increased C flow into actinomycetes with elevated CO2. Further, the fourfold increase in PLFA‐based SUE for vanillin phenolic‐C in elevated CO2 soils that received N suggests future enhanced N limitation in elevated CO2 soils may promote enhanced respiratory loss relative to incorporation of some C‐substrates into microbial biomass. These short‐term incubations did not reveal greater loss of soil organic carbon via respiration or shifts in SUE with elevated CO2. However, observed relative increases in activity of actinomycetes and fungi with elevated CO2 and mitigation of this effect on actinomycetes with N amendments suggests that elevated CO2 and predicted N limitation may alter the fate of slow‐turnover soil organic matter (SOM) in two competing ways. Investigations need to focus on how these microorganisms may increase slow‐turnover substrate use while possibly enhancing the prevalence of microbial cell wall structures that can serve as precursors of stabilized SOM.</p></abstract><textClass><keywords scheme="keyword"><list><head>keywords</head><item><term>soil organic carbon</term></item><item><term>heterotrophic respiration</term></item><item><term>elevated CO2</term></item><item><term>progressive N limitation</term></item><item><term>phospholipid fatty acids</term></item><item><term>stable carbon isotopes</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>index-terms</head><item><term>BIOGEOSCIENCES</term></item><item><term>Soils/pedology</term></item><item><term>Carbon cycling</term></item><item><term>Nitrogen cycling</term></item><item><term>Isotopic composition and chemistry</term></item><item><term>Microbiology: ecology, physiology and genomics</term></item><item><term>GEOCHEMISTRY</term></item><item><term>Stable isotope geochemistry</term></item><item><term>HYDROLOGY</term></item><item><term>Soils</term></item><item><term>OCEANOGRAPHY: BIOLOGICAL AND CHEMICAL</term></item><item><term>Carbon cycling</term></item><item><term>Stable isotopes</term></item><item><term>Microbiology and microbial ecology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2010-05-20">Received</change><change when="2010-11-17">Registration</change><change when="2011-03">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api-v5.istex.fr/document/76992B6B96AC9A9314A96D5789519562A4E97ED5/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component type="serialArticle" version="2.0" xml:lang="en" xml:id="jgrg720"><header><publicationMeta level="product"><doi>10.1002/(ISSN)2156-2202g</doi><issn type="print">0148-0227</issn><issn type="electronic">2156-2202</issn><idGroup><id type="product" value="JGRG"></id><id type="coden" value="JGREA2"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="JOURNAL OF GEOPHYSICAL RESEARCH: BIOGEOSCIENCES">Journal of Geophysical Research: Biogeosciences</title><title type="short">J. 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E.</givenNames></author>, and <author><givenNames>S. A.</givenNames> <familyName>Billings</familyName></author> (<pubYear year="2011">2011</pubYear>), <articleTitle>Soil nitrogen status as a regulator of carbon substrate flows through microbial communities with elevated CO<sub>2</sub></articleTitle>, <journalTitle>J. Geophys. Res.</journalTitle>, <vol>116</vol>, G01011, doi:<accessionId ref="info:doi/10.1029/2010JG001434">10.1029/2010JG001434</accessionId>.</citation></selfCitationGroup><linkGroup><link type="toTypesetVersion" href="file:JGRG.JGRG720.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="wordTotal" number="12500"></count><count type="figureTotal" number="5"></count><count type="tableTotal" number="2"></count></countGroup><titleGroup><title type="main">Soil nitrogen status as a regulator of carbon substrate flows through microbial communities with elevated CO<sub>2</sub></title><title type="short">SOIL C‐SUBSTRATE FLOW WITH ELEVATED CO<sub>2</sub></title><title type="shortAuthors">Ziegler and Billings</title></titleGroup><creators><creator creatorRole="author" xml:id="jgrg720-cr-0001" affiliationRef="#jgrg720-aff-0001"><personName><givenNames>Susan E.</givenNames> <familyName>Ziegler</familyName></personName><contactDetails><email normalForm="sziegler@mun.ca">sziegler@mun.ca</email></contactDetails></creator><creator creatorRole="author" xml:id="jgrg720-cr-0002" affiliationRef="#jgrg720-aff-0002"><personName><givenNames>Sharon A.</givenNames> <familyName>Billings</familyName></personName></creator></creators><affiliationGroup><affiliation countryCode="CA" type="organization" xml:id="jgrg720-aff-0001"><orgDiv>Department of Earth Science</orgDiv><orgName>Memorial University of Newfoundland</orgName><address><city>St. John's, Newfoundland and Labrador</city><country>Canada</country></address></affiliation><affiliation countryCode="US" type="organization" xml:id="jgrg720-aff-0002"><orgDiv>Department of Ecology and Evolutionary Biology and Kansas Biological Survey</orgDiv><orgName>University of Kansas</orgName><address><city>Lawrence</city><countryPart>Kansas</countryPart><country>USA</country></address></affiliation></affiliationGroup><keywordGroup type="author"><keyword xml:id="jgrg720-kwd-0001">soil organic carbon</keyword><keyword xml:id="jgrg720-kwd-0002">heterotrophic respiration</keyword><keyword xml:id="jgrg720-kwd-0003">elevated CO<sub>2</sub></keyword><keyword xml:id="jgrg720-kwd-0004">progressive N limitation</keyword><keyword xml:id="jgrg720-kwd-0005">phospholipid fatty acids</keyword><keyword xml:id="jgrg720-kwd-0006">stable carbon isotopes</keyword></keywordGroup><supportingInformation><supportingInfoItem><mediaResource alt="supplementary data" mimeType="text/plain" href="urn-x:wiley:01480227:media:jgrg720:jgrg720-sup-0001-t01"></mediaResource><caption>Tab‐delimited Table 1.</caption></supportingInfoItem><supportingInfoItem><mediaResource alt="supplementary data" mimeType="text/plain" href="urn-x:wiley:01480227:media:jgrg720:jgrg720-sup-0002-t02"></mediaResource><caption>Tab‐delimited Table 2.</caption></supportingInfoItem></supportingInformation><abstractGroup><abstract type="main"><p xml:id="jgrg720-para-0001" label="1">To assess how microbial processing of organic C inputs to forest soils may be influenced by elevated CO<sub>2</sub> and altered N dynamics, we followed the fate of <sup>13</sup>C‐labeled substrates in soils from the Duke Free Air Carbon Enrichment site where differences in soil N status have been imposed by 7 years of N amendments. Heterotrophic respiration and <i>δ</i><sup>13</sup>C of respired CO<sub>2</sub>‐C and phospholipid fatty acids (PLFA) were measured to track activities of microbial groups and estimate a relative measure of substrate use efficiency (PLFA‐based SUE). Results indicate an increased proportion of fungal and actinomycete activity in elevated CO<sub>2</sub> soils, which varied with substrate. The negative effect of N on vanillin phenolic‐C incorporation into actinomycete PLFA suggests legacies of fertilization can mitigate increased C flow into actinomycetes with elevated CO<sub>2</sub>. Further, the fourfold increase in PLFA‐based SUE for vanillin phenolic‐C in elevated CO<sub>2</sub> soils that received N suggests future enhanced N limitation in elevated CO<sub>2</sub> soils may promote enhanced respiratory loss relative to incorporation of some C‐substrates into microbial biomass. These short‐term incubations did not reveal greater loss of soil organic carbon via respiration or shifts in SUE with elevated CO<sub>2</sub>. However, observed relative increases in activity of actinomycetes and fungi with elevated CO<sub>2</sub> and mitigation of this effect on actinomycetes with N amendments suggests that elevated CO<sub>2</sub> and predicted N limitation may alter the fate of slow‐turnover soil organic matter (SOM) in two competing ways. Investigations need to focus on how these microorganisms may increase slow‐turnover substrate use while possibly enhancing the prevalence of microbial cell wall structures that can serve as precursors of stabilized SOM.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Soil nitrogen status as a regulator of carbon substrate flows through microbial communities with elevated CO2</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>SOIL C‐SUBSTRATE FLOW WITH ELEVATED CO2</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Soil nitrogen status as a regulator of carbon substrate flows through microbial communities with elevated CO2</title></titleInfo><name type="personal"><namePart type="given">Susan E.</namePart><namePart type="family">Ziegler</namePart><affiliation>Department of Earth Science, Memorial University of Newfoundland, St. John's, Newfoundland and Labrador, Canada</affiliation><affiliation>E-mail: sziegler@mun.ca</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sharon A.</namePart><namePart type="family">Billings</namePart><affiliation>Department of Ecology and Evolutionary Biology and Kansas Biological Survey, University of Kansas, Kansas, Lawrence, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><dateIssued encoding="w3cdtf">2011-03</dateIssued><dateCaptured encoding="w3cdtf">2010-05-20</dateCaptured><dateValid encoding="w3cdtf">2010-11-17</dateValid><edition>Ziegler, S. E., and S. A. Billings (2011), Soil nitrogen status as a regulator of carbon substrate flows through microbial communities with elevated CO2, J. Geophys. Res., 116, G01011, doi:10.1029/2010JG001434.</edition><copyrightDate encoding="w3cdtf">2011</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">5</extent><extent unit="tables">2</extent><extent unit="words">12500</extent></physicalDescription><abstract>To assess how microbial processing of organic C inputs to forest soils may be influenced by elevated CO2 and altered N dynamics, we followed the fate of 13C‐labeled substrates in soils from the Duke Free Air Carbon Enrichment site where differences in soil N status have been imposed by 7 years of N amendments. Heterotrophic respiration and δ13C of respired CO2‐C and phospholipid fatty acids (PLFA) were measured to track activities of microbial groups and estimate a relative measure of substrate use efficiency (PLFA‐based SUE). Results indicate an increased proportion of fungal and actinomycete activity in elevated CO2 soils, which varied with substrate. The negative effect of N on vanillin phenolic‐C incorporation into actinomycete PLFA suggests legacies of fertilization can mitigate increased C flow into actinomycetes with elevated CO2. Further, the fourfold increase in PLFA‐based SUE for vanillin phenolic‐C in elevated CO2 soils that received N suggests future enhanced N limitation in elevated CO2 soils may promote enhanced respiratory loss relative to incorporation of some C‐substrates into microbial biomass. These short‐term incubations did not reveal greater loss of soil organic carbon via respiration or shifts in SUE with elevated CO2. However, observed relative increases in activity of actinomycetes and fungi with elevated CO2 and mitigation of this effect on actinomycetes with N amendments suggests that elevated CO2 and predicted N limitation may alter the fate of slow‐turnover soil organic matter (SOM) in two competing ways. Investigations need to focus on how these microorganisms may increase slow‐turnover substrate use while possibly enhancing the prevalence of microbial cell wall structures that can serve as precursors of stabilized SOM.</abstract><note type="additional physical form">Tab‐delimited Table 1.Tab‐delimited Table 2.</note><subject><genre>keywords</genre><topic>soil organic carbon</topic><topic>heterotrophic respiration</topic><topic>elevated CO2</topic><topic>progressive N limitation</topic><topic>phospholipid fatty acids</topic><topic>stable carbon isotopes</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Geophysical Research: Biogeosciences</title></titleInfo><titleInfo type="abbreviated"><title>J. Geophys. Res.</title></titleInfo><genre type="journal">journal</genre><subject><genre>index-terms</genre><topic authorityURI="http://psi.agu.org/taxonomy5/0400">BIOGEOSCIENCES</topic><topic authorityURI="http://psi.agu.org/taxonomy5/0486">Soils/pedology</topic><topic authorityURI="http://psi.agu.org/taxonomy5/0428">Carbon cycling</topic><topic authorityURI="http://psi.agu.org/taxonomy5/0469">Nitrogen cycling</topic><topic authorityURI="http://psi.agu.org/taxonomy5/0454">Isotopic composition and chemistry</topic><topic authorityURI="http://psi.agu.org/taxonomy5/0465">Microbiology: ecology, physiology and genomics</topic><topic authorityURI="http://psi.agu.org/taxonomy5/1000">GEOCHEMISTRY</topic><topic authorityURI="http://psi.agu.org/taxonomy5/1041">Stable isotope geochemistry</topic><topic authorityURI="http://psi.agu.org/taxonomy5/1800">HYDROLOGY</topic><topic authorityURI="http://psi.agu.org/taxonomy5/1865">Soils</topic><topic authorityURI="http://psi.agu.org/taxonomy5/4800">OCEANOGRAPHY: BIOLOGICAL AND CHEMICAL</topic><topic authorityURI="http://psi.agu.org/taxonomy5/4806">Carbon cycling</topic><topic authorityURI="http://psi.agu.org/taxonomy5/4870">Stable isotopes</topic><topic authorityURI="http://psi.agu.org/taxonomy5/4840">Microbiology and microbial ecology</topic></subject><identifier type="ISSN">0148-0227</identifier><identifier type="eISSN">2156-2202</identifier><identifier type="DOI">10.1002/(ISSN)2156-2202g</identifier><identifier type="CODEN">JGREA2</identifier><identifier type="PublisherID">JGRG</identifier><part><date>2011</date><detail type="volume"><caption>vol.</caption><number>116</number></detail><detail type="issue"><caption>no.</caption><number>G1</number></detail><extent unit="pages"><start>n/a</start><end>n/a</end><total>14</total></extent></part></relatedItem><identifier type="istex">76992B6B96AC9A9314A96D5789519562A4E97ED5</identifier><identifier type="DOI">10.1029/2010JG001434</identifier><identifier type="ArticleID">2010JG001434</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright 2011 by the American Geophysical Union.</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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