Wheat extracts as an efficient cryoprotective agent for primary cultures of rat hepatocytes
Identifieur interne : 001625 ( Istex/Corpus ); précédent : 001624; suivant : 001626Wheat extracts as an efficient cryoprotective agent for primary cultures of rat hepatocytes
Auteurs : Francine Hamel ; Mélanie Grondin ; Francine Denizeau ; Diana A. Averill-Bates ; Fathey SarhanSource :
- Biotechnology and Bioengineering [ 0006-3592 ] ; 2006-11-05.
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Abstract
Hepatocytes are an important physiological model for evaluation of metabolic and biological effects of xenobiotics. They do not proliferate in culture and are extremely sensitive to damage during freezing and thawing, even after the addition of classical cryoprotectants. Thus improved cryopreservation techniques are needed to reduce cell injury and functional impairment. Here, we describe a new and efficient cryopreservation method, which permits long‐term storage and recovery of large quantities of healthy cells that maintain high hepatospecific functions. In culture, the morphology of hepatocytes cryopreserved with wheat protein extracts (WPE) was similar to that of fresh cells. Furthermore, hepatospecific functions such as albumin secretion and biotransformation of ammonium to urea were well maintained during 4 days in culture. Inductions of CYP1A1 and CYP2B in hepatocytes cryopreserved with WPEs were similar to those in fresh hepatocytes. These findings clearly show that WPEs are an excellent cryopreservant for primary hepatocytes. The extract was also found to cryopreserve other human and animal cell types such as lung carcinoma, colorectal adenocarcinoma, Chinese hamster ovary transfected with TGF‐b1 cDNA, cervical cancer taken from Henrietta Lacks, intestinal epithelium, and T cell leukemia. WPEs have potential as a universal cryopreservant agent of mammalian cells. It is an economic, efficient and non‐toxic agent. © 2006 Wiley Periodicals, Inc.
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DOI: 10.1002/bit.20953
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ISTEX:F59CE0C299A18E31C2A08D49970AE929F7AE3B82Le document en format XML
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They do not proliferate in culture and are extremely sensitive to damage during freezing and thawing, even after the addition of classical cryoprotectants. Thus improved cryopreservation techniques are needed to reduce cell injury and functional impairment. Here, we describe a new and efficient cryopreservation method, which permits long‐term storage and recovery of large quantities of healthy cells that maintain high hepatospecific functions. In culture, the morphology of hepatocytes cryopreserved with wheat protein extracts (WPE) was similar to that of fresh cells. Furthermore, hepatospecific functions such as albumin secretion and biotransformation of ammonium to urea were well maintained during 4 days in culture. Inductions of CYP1A1 and CYP2B in hepatocytes cryopreserved with WPEs were similar to those in fresh hepatocytes. These findings clearly show that WPEs are an excellent cryopreservant for primary hepatocytes. The extract was also found to cryopreserve other human and animal cell types such as lung carcinoma, colorectal adenocarcinoma, Chinese hamster ovary transfected with TGF‐b1 cDNA, cervical cancer taken from Henrietta Lacks, intestinal epithelium, and T cell leukemia. WPEs have potential as a universal cryopreservant agent of mammalian cells. 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The extract was also found to cryopreserve other human and animal cell types such as lung carcinoma, colorectal adenocarcinoma, Chinese hamster ovary transfected with TGF‐b1 cDNA, cervical cancer taken from Henrietta Lacks, intestinal epithelium, and T cell leukemia. WPEs have potential as a universal cryopreservant agent of mammalian cells. 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telephone: (514) 987‐3000, ext. 3363; fax: (514) 987‐4647</affiliation><affiliation>Département de Chimie, Université du Québec à Montréal, Montréal, Québec, Canada</affiliation><affiliation>Université du Québec à Montréal, Centre TOXEN, Montréal, Québec, Canada</affiliation></author><author xml:id="author-2"><persName><forename type="first">Mélanie</forename><surname>Grondin</surname></persName><affiliation>Département des Sciences biologiques, Université du Québec à Montréal, C.P. 8888, Succursale Centre‐ville, Montréal, Québec H3C 3P8, Canada; telephone: (514) 987‐3000, ext. 3363; fax: (514) 987‐4647</affiliation><affiliation>Département de Chimie, Université du Québec à Montréal, Montréal, Québec, Canada</affiliation><affiliation>Université du Québec à Montréal, Centre TOXEN, Montréal, Québec, Canada</affiliation></author><author xml:id="author-3"><persName><forename type="first">Francine</forename><surname>Denizeau</surname></persName><affiliation>Département de Chimie, Université du Québec à Montréal, Montréal, Québec, Canada</affiliation><affiliation>Université du Québec à Montréal, Centre TOXEN, Montréal, Québec, Canada</affiliation></author><author xml:id="author-4"><persName><forename type="first">Diana A.</forename><surname>Averill‐Bates</surname></persName><affiliation>Département des Sciences biologiques, Université du Québec à Montréal, C.P. 8888, Succursale Centre‐ville, Montréal, Québec H3C 3P8, Canada; 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The extract was also found to cryopreserve other human and animal cell types such as lung carcinoma, colorectal adenocarcinoma, Chinese hamster ovary transfected with TGF‐b1 cDNA, cervical cancer taken from Henrietta Lacks, intestinal epithelium, and T cell leukemia. WPEs have potential as a universal cryopreservant agent of mammalian cells. 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Bioeng.</title></titleGroup></publicationMeta><publicationMeta level="part" position="40"><doi origin="wiley" registered="yes">10.1002/bit.v95:4</doi><numberingGroup><numbering type="journalVolume" number="95">95</numbering><numbering type="journalIssue">4</numbering></numberingGroup><coverDate startDate="2006-11-05">5 November 2006</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="110" status="forIssue"><doi origin="wiley" registered="yes">10.1002/bit.20953</doi><idGroup><id type="unit" value="BIT20953"></id></idGroup><countGroup><count type="pageTotal" number="10"></count></countGroup><titleGroup><title type="articleCategory">Article</title><title type="tocHeading1">Articles</title></titleGroup><copyright ownership="publisher">Copyright © 2006 Wiley Periodicals, Inc., A Wiley Company</copyright><eventGroup><event type="manuscriptReceived" date="2005-12-06"></event><event type="manuscriptAccepted" date="2006-03-13"></event><event type="publishedOnlineEarlyUnpaginated" date="2006-08-21"></event><event type="firstOnline" date="2006-08-21"></event><event type="publishedOnlineFinalForm" date="2006-09-27"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-03-08"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-08"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-15"></event></eventGroup><numberingGroup><numbering type="pageFirst">661</numbering><numbering type="pageLast">670</numbering></numberingGroup><correspondenceTo>Département des Sciences biologiques, Université du Québec à Montréal, C.P. 8888, Succursale Centre‐ville, Montréal, Québec H3C 3P8, Canada; telephone: (514) 987‐3000, ext. 3363; fax: (514) 987‐4647</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:BIT.BIT20953.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="5"></count><count type="tableTotal" number="1"></count><count type="referenceTotal" number="40"></count><count type="wordTotal" number="1208"></count></countGroup><titleGroup><title type="main" xml:lang="en">Wheat extracts as an efficient cryoprotective agent for primary cultures of rat hepatocytes<link href="#fn1"></link></title><title type="short" xml:lang="en">Wheat Extracts, an Efficient Cryoprotective Agent</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1 #af2 #af3" noteRef="#fn2"><personName><givenNames>Francine</givenNames><familyName>Hamel</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af1 #af2 #af3"><personName><givenNames>Mélanie</givenNames><familyName>Grondin</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af2 #af3"><personName><givenNames>Francine</givenNames><familyName>Denizeau</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af1 #af3"><personName><givenNames>Diana A.</givenNames><familyName>Averill‐Bates</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>Fathey</givenNames><familyName>Sarhan</familyName></personName><contactDetails><email>sarhan.fathey@uqam.ca</email></contactDetails></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="CA" type="organization"><unparsedAffiliation>Département des Sciences biologiques, Université du Québec à Montréal, C.P. 8888, Succursale Centre‐ville, Montréal, Québec H3C 3P8, Canada; telephone: (514) 987‐3000, ext. 3363; fax: (514) 987‐4647</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="CA" type="organization"><unparsedAffiliation>Département de Chimie, Université du Québec à Montréal, Montréal, Québec, Canada</unparsedAffiliation></affiliation><affiliation xml:id="af3" countryCode="CA" type="organization"><unparsedAffiliation>Université du Québec à Montréal, Centre TOXEN, Montréal, Québec, Canada</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">liver</keyword><keyword xml:id="kwd2">cryopreservation</keyword><keyword xml:id="kwd3">wheat proteins</keyword><keyword xml:id="kwd4">viability</keyword><keyword xml:id="kwd5">metabolic activities</keyword></keywordGroup><fundingInfo><fundingAgency>Natural Sciences and Engineering Research Council of Canada (NSERC)</fundingAgency></fundingInfo><fundingInfo><fundingAgency>AstraZeneca R&D Montreal</fundingAgency></fundingInfo><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Hepatocytes are an important physiological model for evaluation of metabolic and biological effects of xenobiotics. They do not proliferate in culture and are extremely sensitive to damage during freezing and thawing, even after the addition of classical cryoprotectants. Thus improved cryopreservation techniques are needed to reduce cell injury and functional impairment. Here, we describe a new and efficient cryopreservation method, which permits long‐term storage and recovery of large quantities of healthy cells that maintain high hepatospecific functions. In culture, the morphology of hepatocytes cryopreserved with wheat protein extracts (WPE) was similar to that of fresh cells. Furthermore, hepatospecific functions such as albumin secretion and biotransformation of ammonium to urea were well maintained during 4 days in culture. Inductions of CYP1A1 and CYP2B in hepatocytes cryopreserved with WPEs were similar to those in fresh hepatocytes. These findings clearly show that WPEs are an excellent cryopreservant for primary hepatocytes. The extract was also found to cryopreserve other human and animal cell types such as lung carcinoma, colorectal adenocarcinoma, Chinese hamster ovary transfected with TGF‐b1 cDNA, cervical cancer taken from Henrietta Lacks, intestinal epithelium, and T cell leukemia. WPEs have potential as a universal cryopreservant agent of mammalian cells. It is an economic, efficient and non‐toxic agent. © 2006 Wiley Periodicals, Inc.</p></abstract></abstractGroup></contentMeta><noteGroup><note xml:id="fn1"><p>Francine Hamel and Mélanie Grondin contributed equally to this work.</p></note><note xml:id="fn2"><p>In memory of Professor Francine Denizeau, her endless energy, and her dedication to the pursuit of scientific excellence (March 25, 2004).</p></note></noteGroup></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Wheat extracts as an efficient cryoprotective agent for primary cultures of rat hepatocytes</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Wheat Extracts, an Efficient Cryoprotective Agent</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Wheat extracts as an efficient cryoprotective agent for primary cultures of rat hepatocytes</title></titleInfo><name type="personal"><namePart type="given">Francine</namePart><namePart type="family">Hamel</namePart><affiliation>Département des Sciences biologiques, Université du Québec à Montréal, C.P. 8888, Succursale Centre‐ville, Montréal, Québec H3C 3P8, Canada; telephone: (514) 987‐3000, ext. 3363; fax: (514) 987‐4647</affiliation><affiliation>Département de Chimie, Université du Québec à Montréal, Montréal, Québec, Canada</affiliation><affiliation>Université du Québec à Montréal, Centre TOXEN, Montréal, Québec, Canada</affiliation><description>In memory of Professor Francine Denizeau, her endless energy, and her dedication to the pursuit of scientific excellence (March 25, 2004).</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mélanie</namePart><namePart type="family">Grondin</namePart><affiliation>Département des Sciences biologiques, Université du Québec à Montréal, C.P. 8888, Succursale Centre‐ville, Montréal, Québec H3C 3P8, Canada; telephone: (514) 987‐3000, ext. 3363; fax: (514) 987‐4647</affiliation><affiliation>Département de Chimie, Université du Québec à Montréal, Montréal, Québec, Canada</affiliation><affiliation>Université du Québec à Montréal, Centre TOXEN, Montréal, Québec, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Francine</namePart><namePart type="family">Denizeau</namePart><affiliation>Département de Chimie, Université du Québec à Montréal, Montréal, Québec, Canada</affiliation><affiliation>Université du Québec à Montréal, Centre TOXEN, Montréal, Québec, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Diana A.</namePart><namePart type="family">Averill‐Bates</namePart><affiliation>Département des Sciences biologiques, Université du Québec à Montréal, C.P. 8888, Succursale Centre‐ville, Montréal, Québec H3C 3P8, Canada; telephone: (514) 987‐3000, ext. 3363; fax: (514) 987‐4647</affiliation><affiliation>Université du Québec à Montréal, Centre TOXEN, Montréal, Québec, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Fathey</namePart><namePart type="family">Sarhan</namePart><affiliation>Département des Sciences biologiques, Université du Québec à Montréal, C.P. 8888, Succursale Centre‐ville, Montréal, Québec H3C 3P8, Canada; telephone: (514) 987‐3000, ext. 3363; fax: (514) 987‐4647</affiliation><affiliation>Département des Sciences biologiques, Université du Québec à Montréal, C.P. 8888, Succursale Centre‐ville, Montréal, Québec H3C 3P8, Canada; telephone: (514) 987‐3000, ext. 3363; fax: (514) 987‐4647</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2006-11-05</dateIssued><dateCaptured encoding="w3cdtf">2005-12-06</dateCaptured><dateValid encoding="w3cdtf">2006-03-13</dateValid><copyrightDate encoding="w3cdtf">2006</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">5</extent><extent unit="tables">1</extent><extent unit="references">40</extent><extent unit="words">1208</extent></physicalDescription><abstract lang="en">Hepatocytes are an important physiological model for evaluation of metabolic and biological effects of xenobiotics. They do not proliferate in culture and are extremely sensitive to damage during freezing and thawing, even after the addition of classical cryoprotectants. Thus improved cryopreservation techniques are needed to reduce cell injury and functional impairment. Here, we describe a new and efficient cryopreservation method, which permits long‐term storage and recovery of large quantities of healthy cells that maintain high hepatospecific functions. In culture, the morphology of hepatocytes cryopreserved with wheat protein extracts (WPE) was similar to that of fresh cells. Furthermore, hepatospecific functions such as albumin secretion and biotransformation of ammonium to urea were well maintained during 4 days in culture. Inductions of CYP1A1 and CYP2B in hepatocytes cryopreserved with WPEs were similar to those in fresh hepatocytes. These findings clearly show that WPEs are an excellent cryopreservant for primary hepatocytes. The extract was also found to cryopreserve other human and animal cell types such as lung carcinoma, colorectal adenocarcinoma, Chinese hamster ovary transfected with TGF‐b1 cDNA, cervical cancer taken from Henrietta Lacks, intestinal epithelium, and T cell leukemia. WPEs have potential as a universal cryopreservant agent of mammalian cells. It is an economic, efficient and non‐toxic agent. © 2006 Wiley Periodicals, Inc.</abstract><note type="content">*Francine Hamel and Mélanie Grondin contributed equally to this work.</note><note type="funding">Natural Sciences and Engineering Research Council of Canada (NSERC)</note><note type="funding">AstraZeneca R&D Montreal</note><subject lang="en"><genre>keywords</genre><topic>liver</topic><topic>cryopreservation</topic><topic>wheat proteins</topic><topic>viability</topic><topic>metabolic activities</topic></subject><relatedItem type="host"><titleInfo><title>Biotechnology and Bioengineering</title></titleInfo><titleInfo type="abbreviated"><title>Biotechnol. Bioeng.</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Article</topic></subject><identifier type="ISSN">0006-3592</identifier><identifier type="eISSN">1097-0290</identifier><identifier type="DOI">10.1002/(ISSN)1097-0290</identifier><identifier type="PublisherID">BIT</identifier><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>95</number></detail><detail type="issue"><caption>no.</caption><number>4</number></detail><extent unit="pages"><start>661</start><end>670</end><total>10</total></extent></part></relatedItem><identifier type="istex">F59CE0C299A18E31C2A08D49970AE929F7AE3B82</identifier><identifier type="DOI">10.1002/bit.20953</identifier><identifier type="ArticleID">BIT20953</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2006 Wiley Periodicals, Inc., A Wiley Company</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Subscription Services, Inc., A Wiley Company</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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