Characterization of a low pathogenic avian influenza H5N2 virus isolated from a turkey breeder flock in Manitoba, Canada.
Identifieur interne : 000282 ( Main/Corpus ); précédent : 000281; suivant : 000283Characterization of a low pathogenic avian influenza H5N2 virus isolated from a turkey breeder flock in Manitoba, Canada.
Auteurs : Y. Berhane ; T. Joseph ; H. Kehler ; T. Hisanaga ; C. Embury-Hyatt ; S. Diederich ; K Hooper Mcgreevy ; K. Handel ; C. Cottam-Birt ; J. PasickSource :
- Avian diseases [ 0005-2086 ] ; 2014.
English descriptors
- KwdEn :
- Animals (MeSH), Chick Embryo (MeSH), Cloaca (virology), Disease Outbreaks (veterinary), Female (MeSH), Hemagglutination Inhibition Tests (veterinary), Hemagglutination Tests (veterinary), Influenza A Virus, H5N2 Subtype (genetics), Influenza A Virus, H5N2 Subtype (isolation & purification), Influenza A Virus, H5N2 Subtype (pathogenicity), Influenza in Birds (epidemiology), Influenza in Birds (prevention & control), Influenza in Birds (virology), Male (MeSH), Manitoba (epidemiology), Molecular Sequence Data (MeSH), Oropharynx (virology), Phylogeny (MeSH), Polymerase Chain Reaction (veterinary), Poultry Diseases (epidemiology), Poultry Diseases (prevention & control), Poultry Diseases (virology), Prevalence (MeSH), Sequence Analysis, DNA (veterinary), Seroepidemiologic Studies (MeSH), Specific Pathogen-Free Organisms (MeSH), Turkeys (MeSH), Viral Proteins (genetics), Viral Proteins (metabolism), Virulence (MeSH).
- MESH :
- chemical , genetics : Viral Proteins.
- geographic , epidemiology : Manitoba.
- epidemiology : Influenza in Birds, Poultry Diseases.
- genetics : Influenza A Virus, H5N2 Subtype.
- isolation & purification : Influenza A Virus, H5N2 Subtype.
- chemical , metabolism : Viral Proteins.
- pathogenicity : Influenza A Virus, H5N2 Subtype.
- prevention & control : Influenza in Birds, Poultry Diseases.
- veterinary : Disease Outbreaks, Hemagglutination Inhibition Tests, Hemagglutination Tests, Polymerase Chain Reaction, Sequence Analysis, DNA.
- virology : Cloaca, Influenza in Birds, Oropharynx, Poultry Diseases.
- Animals, Chick Embryo, Female, Male, Molecular Sequence Data, Phylogeny, Prevalence, Seroepidemiologic Studies, Specific Pathogen-Free Organisms, Turkeys, Virulence.
Abstract
In November 2010, an outbreak of avian influenza (AI) due to the H5N2 subtype virus occurred in a turkey breeder farm in northern Manitoba, Canada. The only clinical signs observed were depression, decrease in food consumption, and loss of egg production. The hemagglutinin (HA) cleavage (HA(0)) site of the isolated H5N2 virus was PQRETR/GLF, consistent with low pathogenic AI viruses. The intravenous pathogenicity index of this virus was zero. Whole-genome sequencing of two isolates that originated from two different barns was performed, and both isolates had 100% identical protein sequence in PB2, HA, NP, M1, M2, NS1, and NS2. The remaining gene segments (PB1, PA, and NA) had a single amino-acid difference when compared with each other. The nucleotide and protein sequences of eight gene segments from both isolates showed 99 or greater identity with other AI viruses that have been circulating in free-living aquatic birds in Canada and the United States within the last 10 yr. Phylogenetic analysis of the HA and neuraminidase (NA) gene segments showed that these viruses are closely related to other H5 strains that have been isolated from Manitoba and other parts of Canada. Serologic testing of archived serum samples collected from these turkeys a week before the outbreak showed no evidence of AI infection. In addition, other farms that were located within 3 km radius from the infected farm and farms that had epidemiologic connection with the farm also tested negative for the presence of H5N2 AI virus or antibody. This indicates that the virus might have been introduced to the farm from wild aquatic birds only a short time before detection. Results of this study highlight the importance of early detection and the significance of ongoing Canada-wide surveillance of AI in domestic poultry as well as in wild aquatic birds/ducks.
DOI: 10.1637/10591-061213-Reg.1
PubMed: 24758106
Links to Exploration step
pubmed:24758106Le document en format XML
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Diederich</name></author><author><name sortKey="Mcgreevy, K Hooper" sort="Mcgreevy, K Hooper" uniqKey="Mcgreevy K" first="K Hooper" last="Mcgreevy">K Hooper Mcgreevy</name></author><author><name sortKey="Handel, K" sort="Handel, K" uniqKey="Handel K" first="K" last="Handel">K. Handel</name></author><author><name sortKey="Cottam Birt, C" sort="Cottam Birt, C" uniqKey="Cottam Birt C" first="C" last="Cottam-Birt">C. Cottam-Birt</name></author><author><name sortKey="Pasick, J" sort="Pasick, J" uniqKey="Pasick J" first="J" last="Pasick">J. 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Kehler</name></author><author><name sortKey="Hisanaga, T" sort="Hisanaga, T" uniqKey="Hisanaga T" first="T" last="Hisanaga">T. Hisanaga</name></author><author><name sortKey="Embury Hyatt, C" sort="Embury Hyatt, C" uniqKey="Embury Hyatt C" first="C" last="Embury-Hyatt">C. Embury-Hyatt</name></author><author><name sortKey="Diederich, S" sort="Diederich, S" uniqKey="Diederich S" first="S" last="Diederich">S. Diederich</name></author><author><name sortKey="Mcgreevy, K Hooper" sort="Mcgreevy, K Hooper" uniqKey="Mcgreevy K" first="K Hooper" last="Mcgreevy">K Hooper Mcgreevy</name></author><author><name sortKey="Handel, K" sort="Handel, K" uniqKey="Handel K" first="K" last="Handel">K. Handel</name></author><author><name sortKey="Cottam Birt, C" sort="Cottam Birt, C" uniqKey="Cottam Birt C" first="C" last="Cottam-Birt">C. Cottam-Birt</name></author><author><name sortKey="Pasick, J" sort="Pasick, J" uniqKey="Pasick J" first="J" last="Pasick">J. 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The only clinical signs observed were depression, decrease in food consumption, and loss of egg production. The hemagglutinin (HA) cleavage (HA(0)) site of the isolated H5N2 virus was PQRETR/GLF, consistent with low pathogenic AI viruses. The intravenous pathogenicity index of this virus was zero. Whole-genome sequencing of two isolates that originated from two different barns was performed, and both isolates had 100% identical protein sequence in PB2, HA, NP, M1, M2, NS1, and NS2. The remaining gene segments (PB1, PA, and NA) had a single amino-acid difference when compared with each other. The nucleotide and protein sequences of eight gene segments from both isolates showed 99 or greater identity with other AI viruses that have been circulating in free-living aquatic birds in Canada and the United States within the last 10 yr. Phylogenetic analysis of the HA and neuraminidase (NA) gene segments showed that these viruses are closely related to other H5 strains that have been isolated from Manitoba and other parts of Canada. Serologic testing of archived serum samples collected from these turkeys a week before the outbreak showed no evidence of AI infection. In addition, other farms that were located within 3 km radius from the infected farm and farms that had epidemiologic connection with the farm also tested negative for the presence of H5N2 AI virus or antibody. This indicates that the virus might have been introduced to the farm from wild aquatic birds only a short time before detection. Results of this study highlight the importance of early detection and the significance of ongoing Canada-wide surveillance of AI in domestic poultry as well as in wild aquatic birds/ducks.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24758106</PMID><DateCompleted><Year>2014</Year><Month>05</Month><Day>13</Day></DateCompleted><DateRevised><Year>2014</Year><Month>04</Month><Day>24</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0005-2086</ISSN><JournalIssue CitedMedium="Print"><Volume>58</Volume><Issue>1</Issue><PubDate><Year>2014</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Avian diseases</Title><ISOAbbreviation>Avian Dis.</ISOAbbreviation></Journal><ArticleTitle>Characterization of a low pathogenic avian influenza H5N2 virus isolated from a turkey breeder flock in Manitoba, Canada.</ArticleTitle><Pagination><MedlinePgn>1-7</MedlinePgn></Pagination><Abstract><AbstractText>In November 2010, an outbreak of avian influenza (AI) due to the H5N2 subtype virus occurred in a turkey breeder farm in northern Manitoba, Canada. The only clinical signs observed were depression, decrease in food consumption, and loss of egg production. The hemagglutinin (HA) cleavage (HA(0)) site of the isolated H5N2 virus was PQRETR/GLF, consistent with low pathogenic AI viruses. The intravenous pathogenicity index of this virus was zero. Whole-genome sequencing of two isolates that originated from two different barns was performed, and both isolates had 100% identical protein sequence in PB2, HA, NP, M1, M2, NS1, and NS2. The remaining gene segments (PB1, PA, and NA) had a single amino-acid difference when compared with each other. The nucleotide and protein sequences of eight gene segments from both isolates showed 99 or greater identity with other AI viruses that have been circulating in free-living aquatic birds in Canada and the United States within the last 10 yr. Phylogenetic analysis of the HA and neuraminidase (NA) gene segments showed that these viruses are closely related to other H5 strains that have been isolated from Manitoba and other parts of Canada. Serologic testing of archived serum samples collected from these turkeys a week before the outbreak showed no evidence of AI infection. In addition, other farms that were located within 3 km radius from the infected farm and farms that had epidemiologic connection with the farm also tested negative for the presence of H5N2 AI virus or antibody. This indicates that the virus might have been introduced to the farm from wild aquatic birds only a short time before detection. Results of this study highlight the importance of early detection and the significance of ongoing Canada-wide surveillance of AI in domestic poultry as well as in wild aquatic birds/ducks.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Berhane</LastName><ForeName>Y</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Joseph</LastName><ForeName>T</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Kehler</LastName><ForeName>H</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Hisanaga</LastName><ForeName>T</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Embury-Hyatt</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Diederich</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>McGreevy</LastName><ForeName>K Hooper</ForeName><Initials>KH</Initials></Author><Author 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