Physiological responses of Manila clams Venerupis (=Ruditapes) philippinarum with varying parasite Perkinsus olseni burden to toxic algal Alexandrium ostenfeldii exposure
Identifieur interne : 000078 ( Main/Corpus ); précédent : 000077; suivant : 000079Physiological responses of Manila clams Venerupis (=Ruditapes) philippinarum with varying parasite Perkinsus olseni burden to toxic algal Alexandrium ostenfeldii exposure
Auteurs : RBID : Pascal:14-0214668Descripteurs français
- Pascal (Inist)
- Clam, Bivalvia, Association toxique, Parasite, Histopathologie, Toxicité, Algae, Algue nuisible, Stress oxydatif, Activité enzymatique, Antioxydant, Digestion, France, Gironde, Coque (mollusque comestible), Origine animale, Dinophyta, Mollusque comestible, Système immunitaire, Venerupis philippinarum, Perkinsus olseni, Alexandrium ostenfeldii, Immunotoxicité, Bassin Arcachon.
English descriptors
- KwdEn :
Abstract
Manila clam stock from Arcachon Bay, France, is declining, as is commercial harvest. To understand the role of environmental biotic interactions in this decrease, effects of a toxic dinoflagellate, Alexandrium ostenfeldii, which blooms regularly in Arcachon bay, and the interaction with perkinsosis on clam physiology were investigated. Manila clams from Arcachon Bay, with variable natural levels of perkinsosis, were exposed for seven days to a mix of the nutritious microalga T-Iso and the toxic dinoflagellate A. ostenfeldii, a producer of spirolides, followed by seven days of depuration fed only T-Iso. Following sacrifice and quantification of protozoan parasite Perkinsus olseni burden, clams were divided into two groups according to intensity of the infection ("Light-Moderate" and "Moderate-Heavy"). Hemocyte and plasma responses, digestive enzyme activities, antioxidant enzyme activities in gills, and histopathological responses were analyzed. Reactive oxygen species (ROS) production in hemocytes and catalase (CAT) activity in gills increased with P. olseni intensity of infection in control clams fed T-Iso, but did not vary among A. ostenfeldii-exposed clams. Exposure to A. ostenfeldii caused tissue alterations associated with an inflammatory response and modifications in hemocyte morphology. In the gills, superoxide dismutase (SOD) activity decreased, and an increase in brown cell occurrence was seen, suggesting oxidative stress. Observations of hemocytes and brown cells in tissues during exposure and depuration suggest involvement of both cell types in detoxication processes. Results suggest that exposure to A. ostenfeldii disrupted the pro-/anti-oxidant response of clams to heavy P. olseni intensity. In addition, depressed mitochondrial membrane potential (MMP) in hemocytes of clams exposed to A. ostenfeldii suggests that mitochondrial functions are regulated to maintain homeostasis of digestive enzyme activity and condition index.
Notice en format standard (ISO 2709)
Pour connaître la documentation sur le format Inist Standard.
| pA |
|
|---|
Format Inist (serveur)
| NO : | PASCAL 14-0214668 INIST |
|---|---|
| ET : | Physiological responses of Manila clams Venerupis (=Ruditapes) philippinarum with varying parasite Perkinsus olseni burden to toxic algal Alexandrium ostenfeldii exposure |
| AU : | LASSUDRIE (Malwenn); SOUDANT (Philippe); RICHARD (Gaëlle); HENRY (Nicolas); MEDHIOUB (Walid); DA SILVA (Patricia Mirella); DONVAL (Anne); BUNEL (Mélanie); LE GOÏC (Nelly); LAMBERT (Christophe); DE MONTAUDOUIN (Xavier); FABIOUX (Caroline); HEGARET (Hélène) |
| AF : | Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), UBO/CNRS, rue Dumont d'Urville, technopôle Brest-Iroise/29280 Plouzané/France (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 7 aut., 8 aut., 9 aut., 10 aut., 12 aut., 13 aut.); Laboratoire Phycotoxines, Ifremer, Institut Français de Recherche pour l'Exploitation de la Mer, BP 21105/44311 Nantes/France (5 aut.); Laboratoire Milieu Marin, INSTM, Institut National des Sciences et Technologies de la Mer, 28 rue du 2 mars 1934/2025 Salammbô/Tunisie (5 aut.); Laboratory of Immunology and Pathology of Invertebrates, Department of Molecular Biology, Exact and Natural Sciences Center, Federal University of Paraíba-Campus I/58051-900 João Pessoa, PB/Brésil (6 aut.); Université de Bordeaux UMR 5805 EPOC, station marine d'Arcachon, 2 rue du Pr Jolyet/33120 Arcachon/France (11 aut.) |
| DT : | Publication en série; Niveau analytique |
| SO : | Aquatic toxicology; ISSN 0166-445X; Coden AQTODG; Pays-Bas; Da. 2014; Vol. 154; Pp. 27-38; Bibl. 1 p.3/4 |
| LA : | Anglais |
| EA : | Manila clam stock from Arcachon Bay, France, is declining, as is commercial harvest. To understand the role of environmental biotic interactions in this decrease, effects of a toxic dinoflagellate, Alexandrium ostenfeldii, which blooms regularly in Arcachon bay, and the interaction with perkinsosis on clam physiology were investigated. Manila clams from Arcachon Bay, with variable natural levels of perkinsosis, were exposed for seven days to a mix of the nutritious microalga T-Iso and the toxic dinoflagellate A. ostenfeldii, a producer of spirolides, followed by seven days of depuration fed only T-Iso. Following sacrifice and quantification of protozoan parasite Perkinsus olseni burden, clams were divided into two groups according to intensity of the infection ("Light-Moderate" and "Moderate-Heavy"). Hemocyte and plasma responses, digestive enzyme activities, antioxidant enzyme activities in gills, and histopathological responses were analyzed. Reactive oxygen species (ROS) production in hemocytes and catalase (CAT) activity in gills increased with P. olseni intensity of infection in control clams fed T-Iso, but did not vary among A. ostenfeldii-exposed clams. Exposure to A. ostenfeldii caused tissue alterations associated with an inflammatory response and modifications in hemocyte morphology. In the gills, superoxide dismutase (SOD) activity decreased, and an increase in brown cell occurrence was seen, suggesting oxidative stress. Observations of hemocytes and brown cells in tissues during exposure and depuration suggest involvement of both cell types in detoxication processes. Results suggest that exposure to A. ostenfeldii disrupted the pro-/anti-oxidant response of clams to heavy P. olseni intensity. In addition, depressed mitochondrial membrane potential (MMP) in hemocytes of clams exposed to A. ostenfeldii suggests that mitochondrial functions are regulated to maintain homeostasis of digestive enzyme activity and condition index. |
| CC : | 002A14D05G |
| FD : | Clam; Bivalvia; Association toxique; Parasite; Histopathologie; Toxicité; Algae; Algue nuisible; Stress oxydatif; Activité enzymatique; Antioxydant; Digestion; France; Gironde; Coque (mollusque comestible); Origine animale; Dinophyta; Mollusque comestible; Système immunitaire; Venerupis philippinarum; Perkinsus olseni; Alexandrium ostenfeldii; Immunotoxicité; Bassin Arcachon |
| FG : | Mollusca; Invertebrata; Europe; Aquitaine; Apicomplexa; Protozoa; Alveolata; Protiste |
| ED : | Clam; Bivalvia; Toxic association; Parasite; Histopathology; Toxicity; Algae; Harmful alga; Oxidative stress; Enzymatic activity; Antioxidant; Digestion; France; Gironde; Cockle; Animal origin; Dinophyta; Edible mollusc; Immune system |
| EG : | Mollusca; Invertebrata; Europe; Aquitaine; Apicomplexa; Protozoa |
| SD : | Clam; Bivalvia; Asociación toxíca; Parásito; Histopatología; Toxicidad; Algae; Alga nociva; Estrés oxidativo; Actividad enzimática; Antioxidante; Digestión; Francia; Gironde; Berberecho; Origen animal; Dinophyta; Molusco comestible; Sistema inmunitario |
| LO : | INIST-18841.354000150318920040 |
| ID : | 14-0214668 |
Links to Exploration step
Pascal:14-0214668Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Physiological responses of Manila clams Venerupis (=Ruditapes) philippinarum with varying parasite Perkinsus olseni burden to toxic algal Alexandrium ostenfeldii exposure</title><author><name sortKey="Lassudrie, Malwenn" uniqKey="Lassudrie M">Malwenn Lassudrie</name><affiliation><inist:fA14 i1="01"><s1>Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), UBO/CNRS, rue Dumont d'Urville, technopôle Brest-Iroise</s1><s2>29280 Plouzané</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Soudant, Philippe" uniqKey="Soudant P">Philippe Soudant</name><affiliation><inist:fA14 i1="01"><s1>Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), UBO/CNRS, rue Dumont d'Urville, technopôle Brest-Iroise</s1><s2>29280 Plouzané</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Richard, Gaelle" uniqKey="Richard G">Gaëlle Richard</name><affiliation><inist:fA14 i1="01"><s1>Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), UBO/CNRS, rue Dumont d'Urville, technopôle Brest-Iroise</s1><s2>29280 Plouzané</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Henry, Nicolas" uniqKey="Henry N">Nicolas Henry</name><affiliation><inist:fA14 i1="01"><s1>Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), UBO/CNRS, rue Dumont d'Urville, technopôle Brest-Iroise</s1><s2>29280 Plouzané</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Medhioub, Walid" uniqKey="Medhioub W">Walid Medhioub</name><affiliation><inist:fA14 i1="01"><s1>Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), UBO/CNRS, rue Dumont d'Urville, technopôle Brest-Iroise</s1><s2>29280 Plouzané</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="02"><s1>Laboratoire Phycotoxines, Ifremer, Institut Français de Recherche pour l'Exploitation de la Mer, BP 21105</s1><s2>44311 Nantes</s2><s3>FRA</s3><sZ>5 aut.</sZ></inist:fA14></affiliation><affiliation><inist:fA14 i1="03"><s1>Laboratoire Milieu Marin, INSTM, Institut National des Sciences et Technologies de la Mer, 28 rue du 2 mars 1934</s1><s2>2025 Salammbô</s2><s3>TUN</s3><sZ>5 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Da Silva, Patricia Mirella" uniqKey="Da Silva P">Patricia Mirella Da Silva</name><affiliation><inist:fA14 i1="04"><s1>Laboratory of Immunology and Pathology of Invertebrates, Department of Molecular Biology, Exact and Natural Sciences Center, Federal University of Paraíba-Campus I</s1><s2>58051-900 João Pessoa, PB</s2><s3>BRA</s3><sZ>6 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Donval, Anne" uniqKey="Donval A">Anne Donval</name><affiliation><inist:fA14 i1="01"><s1>Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), UBO/CNRS, rue Dumont d'Urville, technopôle Brest-Iroise</s1><s2>29280 Plouzané</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Bunel, Melanie" uniqKey="Bunel M">Mélanie Bunel</name><affiliation><inist:fA14 i1="01"><s1>Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), UBO/CNRS, rue Dumont d'Urville, technopôle Brest-Iroise</s1><s2>29280 Plouzané</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Le Goic, Nelly" uniqKey="Le Goic N">Nelly Le Goïc</name><affiliation><inist:fA14 i1="01"><s1>Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), UBO/CNRS, rue Dumont d'Urville, technopôle Brest-Iroise</s1><s2>29280 Plouzané</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Lambert, Christophe" uniqKey="Lambert C">Christophe Lambert</name><affiliation><inist:fA14 i1="01"><s1>Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), UBO/CNRS, rue Dumont d'Urville, technopôle Brest-Iroise</s1><s2>29280 Plouzané</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="De Montaudouin, Xavier" uniqKey="De Montaudouin X">Xavier De Montaudouin</name><affiliation><inist:fA14 i1="05"><s1>Université de Bordeaux UMR 5805 EPOC, station marine d'Arcachon, 2 rue du Pr Jolyet</s1><s2>33120 Arcachon</s2><s3>FRA</s3><sZ>11 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Fabioux, Caroline" uniqKey="Fabioux C">Caroline Fabioux</name><affiliation><inist:fA14 i1="01"><s1>Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), UBO/CNRS, rue Dumont d'Urville, technopôle Brest-Iroise</s1><s2>29280 Plouzané</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Hegaret, Helene" uniqKey="Hegaret H">Hélène Hegaret</name><affiliation><inist:fA14 i1="01"><s1>Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), UBO/CNRS, rue Dumont d'Urville, technopôle Brest-Iroise</s1><s2>29280 Plouzané</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14></affiliation></author></titleStmt><publicationStmt><idno type="inist">14-0214668</idno><date when="2014">2014</date><idno type="stanalyst">PASCAL 14-0214668 INIST</idno><idno type="RBID">Pascal:14-0214668</idno><idno type="wicri:Area/Main/Corpus">000078</idno></publicationStmt><seriesStmt><idno type="ISSN">0166-445X</idno><title level="j" type="abbreviated">Aquat. toxicol.</title><title level="j" type="main">Aquatic toxicology</title></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Algae</term><term>Animal origin</term><term>Antioxidant</term><term>Bivalvia</term><term>Clam</term><term>Cockle</term><term>Digestion</term><term>Dinophyta</term><term>Edible mollusc</term><term>Enzymatic activity</term><term>France</term><term>Gironde</term><term>Harmful alga</term><term>Histopathology</term><term>Immune system</term><term>Oxidative stress</term><term>Parasite</term><term>Toxic association</term><term>Toxicity</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Clam</term><term>Bivalvia</term><term>Association toxique</term><term>Parasite</term><term>Histopathologie</term><term>Toxicité</term><term>Algae</term><term>Algue nuisible</term><term>Stress oxydatif</term><term>Activité enzymatique</term><term>Antioxydant</term><term>Digestion</term><term>France</term><term>Gironde</term><term>Coque (mollusque comestible)</term><term>Origine animale</term><term>Dinophyta</term><term>Mollusque comestible</term><term>Système immunitaire</term><term>Venerupis philippinarum</term><term>Perkinsus olseni</term><term>Alexandrium ostenfeldii</term><term>Immunotoxicité</term><term>Bassin Arcachon</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Manila clam stock from Arcachon Bay, France, is declining, as is commercial harvest. To understand the role of environmental biotic interactions in this decrease, effects of a toxic dinoflagellate, Alexandrium ostenfeldii, which blooms regularly in Arcachon bay, and the interaction with perkinsosis on clam physiology were investigated. Manila clams from Arcachon Bay, with variable natural levels of perkinsosis, were exposed for seven days to a mix of the nutritious microalga T-Iso and the toxic dinoflagellate A. ostenfeldii, a producer of spirolides, followed by seven days of depuration fed only T-Iso. Following sacrifice and quantification of protozoan parasite Perkinsus olseni burden, clams were divided into two groups according to intensity of the infection ("Light-Moderate" and "Moderate-Heavy"). Hemocyte and plasma responses, digestive enzyme activities, antioxidant enzyme activities in gills, and histopathological responses were analyzed. Reactive oxygen species (ROS) production in hemocytes and catalase (CAT) activity in gills increased with P. olseni intensity of infection in control clams fed T-Iso, but did not vary among A. ostenfeldii-exposed clams. Exposure to A. ostenfeldii caused tissue alterations associated with an inflammatory response and modifications in hemocyte morphology. In the gills, superoxide dismutase (SOD) activity decreased, and an increase in brown cell occurrence was seen, suggesting oxidative stress. Observations of hemocytes and brown cells in tissues during exposure and depuration suggest involvement of both cell types in detoxication processes. Results suggest that exposure to A. ostenfeldii disrupted the pro-/anti-oxidant response of clams to heavy P. olseni intensity. In addition, depressed mitochondrial membrane potential (MMP) in hemocytes of clams exposed to A. ostenfeldii suggests that mitochondrial functions are regulated to maintain homeostasis of digestive enzyme activity and condition index.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0166-445X</s0></fA01><fA02 i1="01"><s0>AQTODG</s0></fA02><fA03 i2="1"><s0>Aquat. toxicol.</s0></fA03><fA05><s2>154</s2></fA05><fA08 i1="01" i2="1" l="ENG"><s1>Physiological responses of Manila clams Venerupis (=Ruditapes) philippinarum with varying parasite Perkinsus olseni burden to toxic algal Alexandrium ostenfeldii exposure</s1></fA08><fA11 i1="01" i2="1"><s1>LASSUDRIE (Malwenn)</s1></fA11><fA11 i1="02" i2="1"><s1>SOUDANT (Philippe)</s1></fA11><fA11 i1="03" i2="1"><s1>RICHARD (Gaëlle)</s1></fA11><fA11 i1="04" i2="1"><s1>HENRY (Nicolas)</s1></fA11><fA11 i1="05" i2="1"><s1>MEDHIOUB (Walid)</s1></fA11><fA11 i1="06" i2="1"><s1>DA SILVA (Patricia Mirella)</s1></fA11><fA11 i1="07" i2="1"><s1>DONVAL (Anne)</s1></fA11><fA11 i1="08" i2="1"><s1>BUNEL (Mélanie)</s1></fA11><fA11 i1="09" i2="1"><s1>LE GOÏC (Nelly)</s1></fA11><fA11 i1="10" i2="1"><s1>LAMBERT (Christophe)</s1></fA11><fA11 i1="11" i2="1"><s1>DE MONTAUDOUIN (Xavier)</s1></fA11><fA11 i1="12" i2="1"><s1>FABIOUX (Caroline)</s1></fA11><fA11 i1="13" i2="1"><s1>HEGARET (Hélène)</s1></fA11><fA14 i1="01"><s1>Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), UBO/CNRS, rue Dumont d'Urville, technopôle Brest-Iroise</s1><s2>29280 Plouzané</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></fA14><fA14 i1="02"><s1>Laboratoire Phycotoxines, Ifremer, Institut Français de Recherche pour l'Exploitation de la Mer, BP 21105</s1><s2>44311 Nantes</s2><s3>FRA</s3><sZ>5 aut.</sZ></fA14><fA14 i1="03"><s1>Laboratoire Milieu Marin, INSTM, Institut National des Sciences et Technologies de la Mer, 28 rue du 2 mars 1934</s1><s2>2025 Salammbô</s2><s3>TUN</s3><sZ>5 aut.</sZ></fA14><fA14 i1="04"><s1>Laboratory of Immunology and Pathology of Invertebrates, Department of Molecular Biology, Exact and Natural Sciences Center, Federal University of Paraíba-Campus I</s1><s2>58051-900 João Pessoa, PB</s2><s3>BRA</s3><sZ>6 aut.</sZ></fA14><fA14 i1="05"><s1>Université de Bordeaux UMR 5805 EPOC, station marine d'Arcachon, 2 rue du Pr Jolyet</s1><s2>33120 Arcachon</s2><s3>FRA</s3><sZ>11 aut.</sZ></fA14><fA20><s1>27-38</s1></fA20><fA21><s1>2014</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>18841</s2><s5>354000150318920040</s5></fA43><fA44><s0>0000</s0><s1>© 2014 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>1 p.3/4</s0></fA45><fA47 i1="01" i2="1"><s0>14-0214668</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Aquatic toxicology</s0></fA64><fA66 i1="01"><s0>NLD</s0></fA66><fC01 i1="01" l="ENG"><s0>Manila clam stock from Arcachon Bay, France, is declining, as is commercial harvest. To understand the role of environmental biotic interactions in this decrease, effects of a toxic dinoflagellate, Alexandrium ostenfeldii, which blooms regularly in Arcachon bay, and the interaction with perkinsosis on clam physiology were investigated. Manila clams from Arcachon Bay, with variable natural levels of perkinsosis, were exposed for seven days to a mix of the nutritious microalga T-Iso and the toxic dinoflagellate A. ostenfeldii, a producer of spirolides, followed by seven days of depuration fed only T-Iso. Following sacrifice and quantification of protozoan parasite Perkinsus olseni burden, clams were divided into two groups according to intensity of the infection ("Light-Moderate" and "Moderate-Heavy"). Hemocyte and plasma responses, digestive enzyme activities, antioxidant enzyme activities in gills, and histopathological responses were analyzed. Reactive oxygen species (ROS) production in hemocytes and catalase (CAT) activity in gills increased with P. olseni intensity of infection in control clams fed T-Iso, but did not vary among A. ostenfeldii-exposed clams. Exposure to A. ostenfeldii caused tissue alterations associated with an inflammatory response and modifications in hemocyte morphology. In the gills, superoxide dismutase (SOD) activity decreased, and an increase in brown cell occurrence was seen, suggesting oxidative stress. Observations of hemocytes and brown cells in tissues during exposure and depuration suggest involvement of both cell types in detoxication processes. Results suggest that exposure to A. ostenfeldii disrupted the pro-/anti-oxidant response of clams to heavy P. olseni intensity. In addition, depressed mitochondrial membrane potential (MMP) in hemocytes of clams exposed to A. ostenfeldii suggests that mitochondrial functions are regulated to maintain homeostasis of digestive enzyme activity and condition index.</s0></fC01><fC02 i1="01" i2="X"><s0>002A14D05G</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Clam</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Clam</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Clam</s0><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Bivalvia</s0><s2>NS</s2><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Bivalvia</s0><s2>NS</s2><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Bivalvia</s0><s2>NS</s2><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Association toxique</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Toxic association</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Asociación toxíca</s0><s5>03</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Parasite</s0><s5>04</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Parasite</s0><s5>04</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Parásito</s0><s5>04</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Histopathologie</s0><s5>05</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Histopathology</s0><s5>05</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Histopatología</s0><s5>05</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Toxicité</s0><s5>06</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Toxicity</s0><s5>06</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Toxicidad</s0><s5>06</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Algae</s0><s2>NS</s2><s5>07</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Algae</s0><s2>NS</s2><s5>07</s5></fC03><fC03 i1="07" i2="X" l="SPA"><s0>Algae</s0><s2>NS</s2><s5>07</s5></fC03><fC03 i1="08" i2="X" l="FRE"><s0>Algue nuisible</s0><s5>08</s5></fC03><fC03 i1="08" i2="X" l="ENG"><s0>Harmful alga</s0><s5>08</s5></fC03><fC03 i1="08" i2="X" l="SPA"><s0>Alga nociva</s0><s5>08</s5></fC03><fC03 i1="09" i2="X" l="FRE"><s0>Stress oxydatif</s0><s5>09</s5></fC03><fC03 i1="09" i2="X" l="ENG"><s0>Oxidative stress</s0><s5>09</s5></fC03><fC03 i1="09" i2="X" l="SPA"><s0>Estrés oxidativo</s0><s5>09</s5></fC03><fC03 i1="10" i2="X" l="FRE"><s0>Activité enzymatique</s0><s5>10</s5></fC03><fC03 i1="10" i2="X" l="ENG"><s0>Enzymatic activity</s0><s5>10</s5></fC03><fC03 i1="10" i2="X" l="SPA"><s0>Actividad enzimática</s0><s5>10</s5></fC03><fC03 i1="11" i2="X" l="FRE"><s0>Antioxydant</s0><s5>11</s5></fC03><fC03 i1="11" i2="X" l="ENG"><s0>Antioxidant</s0><s5>11</s5></fC03><fC03 i1="11" i2="X" l="SPA"><s0>Antioxidante</s0><s5>11</s5></fC03><fC03 i1="12" i2="X" l="FRE"><s0>Digestion</s0><s5>12</s5></fC03><fC03 i1="12" i2="X" l="ENG"><s0>Digestion</s0><s5>12</s5></fC03><fC03 i1="12" i2="X" l="SPA"><s0>Digestión</s0><s5>12</s5></fC03><fC03 i1="13" i2="X" l="FRE"><s0>France</s0><s2>NG</s2><s5>13</s5></fC03><fC03 i1="13" i2="X" l="ENG"><s0>France</s0><s2>NG</s2><s5>13</s5></fC03><fC03 i1="13" i2="X" l="SPA"><s0>Francia</s0><s2>NG</s2><s5>13</s5></fC03><fC03 i1="14" i2="X" l="FRE"><s0>Gironde</s0><s2>NG</s2><s5>14</s5></fC03><fC03 i1="14" i2="X" l="ENG"><s0>Gironde</s0><s2>NG</s2><s5>14</s5></fC03><fC03 i1="14" i2="X" l="SPA"><s0>Gironde</s0><s2>NG</s2><s5>14</s5></fC03><fC03 i1="15" i2="X" l="FRE"><s0>Coque (mollusque comestible)</s0><s5>32</s5></fC03><fC03 i1="15" i2="X" l="ENG"><s0>Cockle</s0><s5>32</s5></fC03><fC03 i1="15" i2="X" l="SPA"><s0>Berberecho</s0><s5>32</s5></fC03><fC03 i1="16" i2="X" l="FRE"><s0>Origine animale</s0><s5>33</s5></fC03><fC03 i1="16" i2="X" l="ENG"><s0>Animal origin</s0><s5>33</s5></fC03><fC03 i1="16" i2="X" l="SPA"><s0>Origen animal</s0><s5>33</s5></fC03><fC03 i1="17" i2="X" l="FRE"><s0>Dinophyta</s0><s2>NS</s2><s5>34</s5></fC03><fC03 i1="17" i2="X" l="ENG"><s0>Dinophyta</s0><s2>NS</s2><s5>34</s5></fC03><fC03 i1="17" i2="X" l="SPA"><s0>Dinophyta</s0><s2>NS</s2><s5>34</s5></fC03><fC03 i1="18" i2="X" l="FRE"><s0>Mollusque comestible</s0><s5>35</s5></fC03><fC03 i1="18" i2="X" l="ENG"><s0>Edible mollusc</s0><s5>35</s5></fC03><fC03 i1="18" i2="X" l="SPA"><s0>Molusco comestible</s0><s5>35</s5></fC03><fC03 i1="19" i2="X" l="FRE"><s0>Système immunitaire</s0><s5>37</s5></fC03><fC03 i1="19" i2="X" l="ENG"><s0>Immune system</s0><s5>37</s5></fC03><fC03 i1="19" i2="X" l="SPA"><s0>Sistema inmunitario</s0><s5>37</s5></fC03><fC03 i1="20" i2="X" l="FRE"><s0>Venerupis philippinarum</s0><s2>NS</s2><s4>INC</s4><s5>76</s5></fC03><fC03 i1="21" i2="X" l="FRE"><s0>Perkinsus olseni</s0><s4>INC</s4><s5>77</s5></fC03><fC03 i1="22" i2="X" l="FRE"><s0>Alexandrium ostenfeldii</s0><s4>INC</s4><s5>78</s5></fC03><fC03 i1="23" i2="X" l="FRE"><s0>Immunotoxicité</s0><s4>INC</s4><s5>81</s5></fC03><fC03 i1="24" i2="X" l="FRE"><s0>Bassin Arcachon</s0><s2>NG</s2><s4>INC</s4><s5>82</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Mollusca</s0><s2>NS</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Mollusca</s0><s2>NS</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Mollusca</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Invertebrata</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Invertebrata</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Invertebrata</s0><s2>NS</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Europe</s0><s2>NG</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Europe</s0><s2>NG</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Europa</s0><s2>NG</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Aquitaine</s0><s2>NG</s2></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Aquitaine</s0><s2>NG</s2></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Aquitaine</s0><s2>NG</s2></fC07><fC07 i1="05" i2="X" l="FRE"><s0>Apicomplexa</s0><s2>NS</s2><s5>53</s5></fC07><fC07 i1="05" i2="X" l="ENG"><s0>Apicomplexa</s0><s2>NS</s2><s5>53</s5></fC07><fC07 i1="05" i2="X" l="SPA"><s0>Apicomplexa</s0><s2>NS</s2><s5>53</s5></fC07><fC07 i1="06" i2="X" l="FRE"><s0>Protozoa</s0><s2>NS</s2></fC07><fC07 i1="06" i2="X" l="ENG"><s0>Protozoa</s0><s2>NS</s2></fC07><fC07 i1="06" i2="X" l="SPA"><s0>Protozoa</s0><s2>NS</s2></fC07><fC07 i1="07" i2="X" l="FRE"><s0>Alveolata</s0><s4>INC</s4><s5>79</s5></fC07><fC07 i1="08" i2="X" l="FRE"><s0>Protiste</s0><s4>INC</s4><s5>80</s5></fC07><fN21><s1>265</s1></fN21></pA></standard><server><NO>PASCAL 14-0214668 INIST</NO><ET>Physiological responses of Manila clams Venerupis (=Ruditapes) philippinarum with varying parasite Perkinsus olseni burden to toxic algal Alexandrium ostenfeldii exposure</ET><AU>LASSUDRIE (Malwenn); SOUDANT (Philippe); RICHARD (Gaëlle); HENRY (Nicolas); MEDHIOUB (Walid); DA SILVA (Patricia Mirella); DONVAL (Anne); BUNEL (Mélanie); LE GOÏC (Nelly); LAMBERT (Christophe); DE MONTAUDOUIN (Xavier); FABIOUX (Caroline); HEGARET (Hélène)</AU><AF>Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), UBO/CNRS, rue Dumont d'Urville, technopôle Brest-Iroise/29280 Plouzané/France (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 7 aut., 8 aut., 9 aut., 10 aut., 12 aut., 13 aut.); Laboratoire Phycotoxines, Ifremer, Institut Français de Recherche pour l'Exploitation de la Mer, BP 21105/44311 Nantes/France (5 aut.); Laboratoire Milieu Marin, INSTM, Institut National des Sciences et Technologies de la Mer, 28 rue du 2 mars 1934/2025 Salammbô/Tunisie (5 aut.); Laboratory of Immunology and Pathology of Invertebrates, Department of Molecular Biology, Exact and Natural Sciences Center, Federal University of Paraíba-Campus I/58051-900 João Pessoa, PB/Brésil (6 aut.); Université de Bordeaux UMR 5805 EPOC, station marine d'Arcachon, 2 rue du Pr Jolyet/33120 Arcachon/France (11 aut.)</AF><DT>Publication en série; Niveau analytique</DT><SO>Aquatic toxicology; ISSN 0166-445X; Coden AQTODG; Pays-Bas; Da. 2014; Vol. 154; Pp. 27-38; Bibl. 1 p.3/4</SO><LA>Anglais</LA><EA>Manila clam stock from Arcachon Bay, France, is declining, as is commercial harvest. To understand the role of environmental biotic interactions in this decrease, effects of a toxic dinoflagellate, Alexandrium ostenfeldii, which blooms regularly in Arcachon bay, and the interaction with perkinsosis on clam physiology were investigated. Manila clams from Arcachon Bay, with variable natural levels of perkinsosis, were exposed for seven days to a mix of the nutritious microalga T-Iso and the toxic dinoflagellate A. ostenfeldii, a producer of spirolides, followed by seven days of depuration fed only T-Iso. Following sacrifice and quantification of protozoan parasite Perkinsus olseni burden, clams were divided into two groups according to intensity of the infection ("Light-Moderate" and "Moderate-Heavy"). Hemocyte and plasma responses, digestive enzyme activities, antioxidant enzyme activities in gills, and histopathological responses were analyzed. Reactive oxygen species (ROS) production in hemocytes and catalase (CAT) activity in gills increased with P. olseni intensity of infection in control clams fed T-Iso, but did not vary among A. ostenfeldii-exposed clams. Exposure to A. ostenfeldii caused tissue alterations associated with an inflammatory response and modifications in hemocyte morphology. In the gills, superoxide dismutase (SOD) activity decreased, and an increase in brown cell occurrence was seen, suggesting oxidative stress. Observations of hemocytes and brown cells in tissues during exposure and depuration suggest involvement of both cell types in detoxication processes. Results suggest that exposure to A. ostenfeldii disrupted the pro-/anti-oxidant response of clams to heavy P. olseni intensity. In addition, depressed mitochondrial membrane potential (MMP) in hemocytes of clams exposed to A. ostenfeldii suggests that mitochondrial functions are regulated to maintain homeostasis of digestive enzyme activity and condition index.</EA><CC>002A14D05G</CC><FD>Clam; Bivalvia; Association toxique; Parasite; Histopathologie; Toxicité; Algae; Algue nuisible; Stress oxydatif; Activité enzymatique; Antioxydant; Digestion; France; Gironde; Coque (mollusque comestible); Origine animale; Dinophyta; Mollusque comestible; Système immunitaire; Venerupis philippinarum; Perkinsus olseni; Alexandrium ostenfeldii; Immunotoxicité; Bassin Arcachon</FD><FG>Mollusca; Invertebrata; Europe; Aquitaine; Apicomplexa; Protozoa; Alveolata; Protiste</FG><ED>Clam; Bivalvia; Toxic association; Parasite; Histopathology; Toxicity; Algae; Harmful alga; Oxidative stress; Enzymatic activity; Antioxidant; Digestion; France; Gironde; Cockle; Animal origin; Dinophyta; Edible mollusc; Immune system</ED><EG>Mollusca; Invertebrata; Europe; Aquitaine; Apicomplexa; Protozoa</EG><SD>Clam; Bivalvia; Asociación toxíca; Parásito; Histopatología; Toxicidad; Algae; Alga nociva; Estrés oxidativo; Actividad enzimática; Antioxidante; Digestión; Francia; Gironde; Berberecho; Origen animal; Dinophyta; Molusco comestible; Sistema inmunitario</SD><LO>INIST-18841.354000150318920040</LO><ID>14-0214668</ID></server></inist></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=FranceBresilV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000078 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000078 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= *** parameter Area/wikiCode missing ***
|area= FranceBresilV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= Pascal:14-0214668
|texte= Physiological responses of Manila clams Venerupis (=Ruditapes) philippinarum with varying parasite Perkinsus olseni burden to toxic algal Alexandrium ostenfeldii exposure
}}
| This area was generated with Dilib version V0.6.01. |  |