Brewhouse-Resident Microbiota Are Responsible for Multi-Stage Fermentation of American Coolship Ale
Identifieur interne : 000E60 ( Main/Exploration ); précédent : 000E59; suivant : 000E61Brewhouse-Resident Microbiota Are Responsible for Multi-Stage Fermentation of American Coolship Ale
Auteurs : Nicholas A. Bokulich [États-Unis] ; Charles W. Bamforth [États-Unis] ; David A. Mills [États-Unis]Source :
- PLoS ONE [ 1932-6203 ] ; 2012.
Abstract
American coolship ale (ACA) is a type of spontaneously fermented beer that employs production methods similar to traditional Belgian lambic. In spite of its growing popularity in the American craft-brewing sector, the fermentation microbiology of ACA has not been previously described, and thus the interface between production methodology and microbial community structure is unexplored. Using terminal restriction fragment length polymorphism (TRFLP), barcoded amplicon sequencing (BAS), quantitative PCR (qPCR) and culture-dependent analysis, ACA fermentations were shown to follow a consistent fermentation progression, initially dominated by
Url:
DOI: 10.1371/journal.pone.0035507
PubMed: 22530036
PubMed Central: 3329477
Affiliations:
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Bokulich</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Viticulture and Enology, Robert Mondavi Institute of Wine and Food Science, University of California Davis, Davis, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Viticulture and Enology, Robert Mondavi Institute of Wine and Food Science, University of California Davis, Davis, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Department of Food Science and Technology, Robert Mondavi Institute of Wine and Food Science, University of California Davis, Davis, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Food Science and Technology, Robert Mondavi Institute of Wine and Food Science, University of California Davis, Davis, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Bamforth, Charles W" sort="Bamforth, Charles W" uniqKey="Bamforth C" first="Charles W." last="Bamforth">Charles W. Bamforth</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Department of Food Science and Technology, Robert Mondavi Institute of Wine and Food Science, University of California Davis, Davis, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Food Science and Technology, Robert Mondavi Institute of Wine and Food Science, University of California Davis, Davis, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Mills, David A" sort="Mills, David A" uniqKey="Mills D" first="David A." last="Mills">David A. Mills</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Viticulture and Enology, Robert Mondavi Institute of Wine and Food Science, University of California Davis, Davis, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Viticulture and Enology, Robert Mondavi Institute of Wine and Food Science, University of California Davis, Davis, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Department of Food Science and Technology, Robert Mondavi Institute of Wine and Food Science, University of California Davis, Davis, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Food Science and Technology, Robert Mondavi Institute of Wine and Food Science, University of California Davis, Davis, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>American coolship ale (ACA) is a type of spontaneously fermented beer that employs production methods similar to traditional Belgian lambic. In spite of its growing popularity in the American craft-brewing sector, the fermentation microbiology of ACA has not been previously described, and thus the interface between production methodology and microbial community structure is unexplored. Using terminal restriction fragment length polymorphism (TRFLP), barcoded amplicon sequencing (BAS), quantitative PCR (qPCR) and culture-dependent analysis, ACA fermentations were shown to follow a consistent fermentation progression, initially dominated by <italic>Enterobacteriaceae</italic> and a range of oxidative yeasts in the first month, then ceding to <italic>Saccharomyces</italic> spp. and <italic>Lactobacillales</italic> for the following year. After one year of fermentation, <italic>Brettanomyces bruxellensis</italic> was the dominant yeast population (occasionally accompanied by minor populations of <italic>Candida</italic> spp., <italic>Pichia</italic> spp., and other yeasts) and <italic>Lactobacillales</italic> remained dominant, though various aerobic bacteria became more prevalent. This work demonstrates that ACA exhibits a conserved core microbial succession in absence of inoculation, supporting the role of a resident brewhouse microbiota. These findings establish this core microbial profile of spontaneous beer fermentations as a target for production control points and quality standards for these beers.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Vanoevelen, D" uniqKey="Vanoevelen D">D Vanoevelen</name></author><author><name sortKey="Spaepen, M" uniqKey="Spaepen M">M Spaepen</name></author><author><name sortKey="Timmermans, P" uniqKey="Timmermans P">P Timmermans</name></author><author><name sortKey="Verachtert, H" uniqKey="Verachtert H">H Verachtert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Verachtert, H" uniqKey="Verachtert H">H Verachtert</name></author><author><name sortKey="Shanta Kumara, Hmc" uniqKey="Shanta Kumara H">HMC Shanta Kumara</name></author><author><name sortKey="Dawoud, E" uniqKey="Dawoud E">E Dawoud</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vanoevelen, D" 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uniqKey="Martini A">A Martini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Santamaria, P" uniqKey="Santamaria P">P Santamaria</name></author><author><name sortKey="Lopez, R" uniqKey="Lopez R">R Lopez</name></author><author><name sortKey="Lopez, E" uniqKey="Lopez E">E Lopez</name></author><author><name sortKey="Garijo, P" uniqKey="Garijo P">P Garijo</name></author><author><name sortKey="Gutierrez, Ar" uniqKey="Gutierrez A">AR Gutierrez</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Bokulich, Nicholas A" sort="Bokulich, Nicholas A" uniqKey="Bokulich N" first="Nicholas A." last="Bokulich">Nicholas A. Bokulich</name></region><name sortKey="Bamforth, Charles W" sort="Bamforth, Charles W" uniqKey="Bamforth C" first="Charles W." last="Bamforth">Charles W. Bamforth</name><name sortKey="Bokulich, Nicholas A" sort="Bokulich, Nicholas A" uniqKey="Bokulich N" first="Nicholas A." last="Bokulich">Nicholas A. Bokulich</name><name sortKey="Mills, David A" sort="Mills, David A" uniqKey="Mills D" first="David A." last="Mills">David A. Mills</name><name sortKey="Mills, David A" sort="Mills, David A" uniqKey="Mills D" first="David A." last="Mills">David A. Mills</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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