Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen.
Identifieur interne : 000C72 ( PubMed/Curation ); précédent : 000C71; suivant : 000C73Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen.
Auteurs : G. L Pez-Torrej N [Espagne] ; M D Ibá Ez ; O. Ahrazem ; R. Sánchez-Monge ; J. Sastre ; M. Lombardero ; D. Barber ; G. SalcedoSource :
- Allergy [ 0105-4538 ] ; 2005.
English descriptors
- KwdEn :
- Adolescent, Adult, Allergens (genetics), Allergens (immunology), Allergens (isolation & purification), Amino Acid Sequence, Antibody Specificity, Antigens, Plant, Child, Chromatography, Affinity, Citrus sinensis (adverse effects), Citrus sinensis (chemistry), Citrus sinensis (immunology), Female, Food Hypersensitivity (blood), Food Hypersensitivity (diagnosis), Food Hypersensitivity (etiology), Humans, Immunoglobulin E (blood), Immunoglobulin E (immunology), Infant, Male, Molecular Sequence Data, Molecular Weight, Plant Proteins (genetics), Plant Proteins (immunology), Plant Proteins (isolation & purification), Profilins (genetics), Profilins (immunology), Profilins (isolation & purification), Sequence Alignment, Skin Tests.
- MESH :
- chemical , blood : Immunoglobulin E.
- chemical , genetics : Allergens, Plant Proteins, Profilins.
- chemical , immunology : Allergens, Immunoglobulin E, Plant Proteins, Profilins.
- chemical , isolation & purification : Allergens, Plant Proteins, Profilins.
- adverse effects : Citrus sinensis.
- blood : Food Hypersensitivity.
- chemistry : Citrus sinensis.
- diagnosis : Food Hypersensitivity.
- etiology : Food Hypersensitivity.
- immunology : Citrus sinensis.
- Adolescent, Adult, Amino Acid Sequence, Antibody Specificity, Antigens, Plant, Child, Chromatography, Affinity, Female, Humans, Infant, Male, Molecular Sequence Data, Molecular Weight, Sequence Alignment, Skin Tests.
Abstract
Orange is among the most widely consumed fruits, and among the plant food sources causing allergic reactions according to popular perception. However, its relevant allergenic components are virtually unknown. Profilin is a well-defined minor plant panallergen, showing prevalences around 30% in fruits and vegetables.
DOI: 10.1111/j.1398-9995.2005.00903.x
PubMed: 16197476
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pubmed:16197476Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen.</title><author><name sortKey="L Pez Torrej N, G" sort="L Pez Torrej N, G" uniqKey="L Pez Torrej N G" first="G" last="L Pez-Torrej N">G. L Pez-Torrej N</name><affiliation wicri:level="1"><nlm:affiliation>Unidad de Bioquímica, Departamento de Biotecnología, E.T.S. Ingenieros Agrónomos, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Unidad de Bioquímica, Departamento de Biotecnología, E.T.S. Ingenieros Agrónomos, Madrid</wicri:regionArea></affiliation></author><author><name sortKey="Iba Ez, M D" sort="Iba Ez, M D" uniqKey="Iba Ez M" first="M D" last="Ibá Ez">M D Ibá Ez</name></author><author><name sortKey="Ahrazem, O" sort="Ahrazem, O" uniqKey="Ahrazem O" first="O" last="Ahrazem">O. Ahrazem</name></author><author><name sortKey="Sanchez Monge, R" sort="Sanchez Monge, R" uniqKey="Sanchez Monge R" first="R" last="Sánchez-Monge">R. Sánchez-Monge</name></author><author><name sortKey="Sastre, J" sort="Sastre, J" uniqKey="Sastre J" first="J" last="Sastre">J. Sastre</name></author><author><name sortKey="Lombardero, M" sort="Lombardero, M" uniqKey="Lombardero M" first="M" last="Lombardero">M. Lombardero</name></author><author><name sortKey="Barber, D" sort="Barber, D" uniqKey="Barber D" first="D" last="Barber">D. Barber</name></author><author><name sortKey="Salcedo, G" sort="Salcedo, G" uniqKey="Salcedo G" first="G" last="Salcedo">G. Salcedo</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16197476</idno><idno type="pmid">16197476</idno><idno type="doi">10.1111/j.1398-9995.2005.00903.x</idno><idno type="wicri:Area/PubMed/Corpus">000C72</idno><idno type="wicri:Area/PubMed/Curation">000C72</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen.</title><author><name sortKey="L Pez Torrej N, G" sort="L Pez Torrej N, G" uniqKey="L Pez Torrej N G" first="G" last="L Pez-Torrej N">G. L Pez-Torrej N</name><affiliation wicri:level="1"><nlm:affiliation>Unidad de Bioquímica, Departamento de Biotecnología, E.T.S. Ingenieros Agrónomos, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Unidad de Bioquímica, Departamento de Biotecnología, E.T.S. Ingenieros Agrónomos, Madrid</wicri:regionArea></affiliation></author><author><name sortKey="Iba Ez, M D" sort="Iba Ez, M D" uniqKey="Iba Ez M" first="M D" last="Ibá Ez">M D Ibá Ez</name></author><author><name sortKey="Ahrazem, O" sort="Ahrazem, O" uniqKey="Ahrazem O" first="O" last="Ahrazem">O. Ahrazem</name></author><author><name sortKey="Sanchez Monge, R" sort="Sanchez Monge, R" uniqKey="Sanchez Monge R" first="R" last="Sánchez-Monge">R. Sánchez-Monge</name></author><author><name sortKey="Sastre, J" sort="Sastre, J" uniqKey="Sastre J" first="J" last="Sastre">J. Sastre</name></author><author><name sortKey="Lombardero, M" sort="Lombardero, M" uniqKey="Lombardero M" first="M" last="Lombardero">M. Lombardero</name></author><author><name sortKey="Barber, D" sort="Barber, D" uniqKey="Barber D" first="D" last="Barber">D. Barber</name></author><author><name sortKey="Salcedo, G" sort="Salcedo, G" uniqKey="Salcedo G" first="G" last="Salcedo">G. Salcedo</name></author></analytic><series><title level="j">Allergy</title><idno type="ISSN">0105-4538</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Allergens (genetics)</term><term>Allergens (immunology)</term><term>Allergens (isolation & purification)</term><term>Amino Acid Sequence</term><term>Antibody Specificity</term><term>Antigens, Plant</term><term>Child</term><term>Chromatography, Affinity</term><term>Citrus sinensis (adverse effects)</term><term>Citrus sinensis (chemistry)</term><term>Citrus sinensis (immunology)</term><term>Female</term><term>Food Hypersensitivity (blood)</term><term>Food Hypersensitivity (diagnosis)</term><term>Food Hypersensitivity (etiology)</term><term>Humans</term><term>Immunoglobulin E (blood)</term><term>Immunoglobulin E (immunology)</term><term>Infant</term><term>Male</term><term>Molecular Sequence Data</term><term>Molecular Weight</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (immunology)</term><term>Plant Proteins (isolation & purification)</term><term>Profilins (genetics)</term><term>Profilins (immunology)</term><term>Profilins (isolation & purification)</term><term>Sequence Alignment</term><term>Skin Tests</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Immunoglobulin E</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Allergens</term><term>Plant Proteins</term><term>Profilins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Allergens</term><term>Immunoglobulin E</term><term>Plant Proteins</term><term>Profilins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Allergens</term><term>Plant Proteins</term><term>Profilins</term></keywords><keywords scheme="MESH" qualifier="adverse effects" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Food Hypersensitivity</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Food Hypersensitivity</term></keywords><keywords scheme="MESH" qualifier="etiology" xml:lang="en"><term>Food Hypersensitivity</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Amino Acid Sequence</term><term>Antibody Specificity</term><term>Antigens, Plant</term><term>Child</term><term>Chromatography, Affinity</term><term>Female</term><term>Humans</term><term>Infant</term><term>Male</term><term>Molecular Sequence Data</term><term>Molecular Weight</term><term>Sequence Alignment</term><term>Skin Tests</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Orange is among the most widely consumed fruits, and among the plant food sources causing allergic reactions according to popular perception. However, its relevant allergenic components are virtually unknown. Profilin is a well-defined minor plant panallergen, showing prevalences around 30% in fruits and vegetables.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16197476</PMID><DateCreated><Year>2005</Year><Month>10</Month><Day>3</Day></DateCreated><DateCompleted><Year>2006</Year><Month>03</Month><Day>23</Day></DateCompleted><DateRevised><Year>2010</Year><Month>11</Month><Day>18</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0105-4538</ISSN><JournalIssue CitedMedium="Print"><Volume>60</Volume><Issue>11</Issue><PubDate><Year>2005</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Allergy</Title><ISOAbbreviation>Allergy</ISOAbbreviation></Journal><ArticleTitle>Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen.</ArticleTitle><Pagination><MedlinePgn>1424-9</MedlinePgn></Pagination><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Orange is among the most widely consumed fruits, and among the plant food sources causing allergic reactions according to popular perception. However, its relevant allergenic components are virtually unknown. Profilin is a well-defined minor plant panallergen, showing prevalences around 30% in fruits and vegetables.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Twenty-three orange-allergic patients were studied. Natural orange profilin, named Cit s 2, was purified by affinity chromatography and characterized by N-terminal amino acid sequencing, matrix-assisted laser desorption/ionization mass spectrometry analysis and isolation of its coding cDNA. Reactivity to Cit s 2 was analyzed in vivo by skin prick tests (SPT) and in vitro by IgE immunodetection, specific IgE determination in individual sera and enzyme-linked immunosorbent assay-inhibition assays.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The N-terminal amino acid sequence and molecular mass of natural Cit s 2, both fully in agreement with the complete amino acid sequence deduced from its coding cDNA, demonstrated its profilin nature. An unexpectedly high reactivity to Cit s 2 was found in vivo (78% of positive SPT responses) and in vitro (87% of sera from orange allergic patients had specific IgE to Cit s 2). The purified allergen inhibited around 50% of the IgE binding to an orange pulp extract.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Orange profilin Cit s 2, unlike other plant food profilins, is a major and highly prevalent allergen.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>López-Torrejón</LastName><ForeName>G</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Unidad de Bioquímica, Departamento de Biotecnología, E.T.S. Ingenieros Agrónomos, Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ibáñez</LastName><ForeName>M D</ForeName><Initials>MD</Initials></Author><Author ValidYN="Y"><LastName>Ahrazem</LastName><ForeName>O</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Sánchez-Monge</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Sastre</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Lombardero</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Barber</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Salcedo</LastName><ForeName>G</ForeName><Initials>G</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Denmark</Country><MedlineTA>Allergy</MedlineTA><NlmUniqueID>7804028</NlmUniqueID><ISSNLinking>0105-4538</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000485">Allergens</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D052179">Antigens, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C508891">Cit s 2 allergen, orange</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D051304">Profilins</NameOfSubstance></Chemical><Chemical><RegistryNumber>37341-29-0</RegistryNumber><NameOfSubstance UI="D007073">Immunoglobulin E</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000485" MajorTopicYN="N">Allergens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000918" MajorTopicYN="N">Antibody Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D052179" MajorTopicYN="N">Antigens, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002648" MajorTopicYN="N">Child</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002846" MajorTopicYN="N">Chromatography, Affinity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000009" MajorTopicYN="Y">adverse effects</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005512" MajorTopicYN="N">Food Hypersensitivity</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000209" MajorTopicYN="Y">etiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007073" MajorTopicYN="N">Immunoglobulin E</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007223" MajorTopicYN="N">Infant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008970" MajorTopicYN="N">Molecular Weight</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051304" MajorTopicYN="N">Profilins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012882" MajorTopicYN="N">Skin Tests</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>10</Month><Day>4</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>3</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>10</Month><Day>4</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16197476</ArticleId><ArticleId IdType="pii">ALL903</ArticleId><ArticleId IdType="doi">10.1111/j.1398-9995.2005.00903.x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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