Isolation and characterization of putative endophytic bacteria antagonistic to Phoma tracheiphila and Verticillium albo-atrum.
Identifieur interne : 000301 ( PubMed/Curation ); précédent : 000300; suivant : 000302Isolation and characterization of putative endophytic bacteria antagonistic to Phoma tracheiphila and Verticillium albo-atrum.
Auteurs : Leila Kalai-Grami [Tunisie] ; Sabrine Saidi ; Sarra Bachkouel ; Imen Ben Slimene ; Monia Mnari-Hattab ; Mohamed Rebah Hajlaoui ; Ferid LimamSource :
- Applied biochemistry and biotechnology [ 1559-0291 ] ; 2014.
English descriptors
- KwdEn :
- Ascomycota, Bacillus (genetics), Bacillus (isolation & purification), Bacillus (metabolism), Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Lipopeptides (genetics), Lipopeptides (metabolism), Peptide Synthases (genetics), Peptide Synthases (metabolism), Plant Diseases, Random Amplified Polymorphic DNA Technique.
- MESH :
- chemical , genetics : Bacterial Proteins, Lipopeptides, Peptide Synthases.
- genetics : Bacillus.
- isolation & purification : Bacillus.
- metabolism : Bacillus, Bacterial Proteins, Lipopeptides, Peptide Synthases.
- Ascomycota, Plant Diseases, Random Amplified Polymorphic DNA Technique.
Abstract
A collection of 200 bacterial isolates recovered from citrus plants (Citrus limon, Citrus sinensis, and Citrus reticulata), Medicago truncatula and Laurus nobilis, was established. In vitro screening indicated that 28 isolates exhibited an inhibitory activity against the vascular pathogens Phoma tracheiphila and Verticillium albo-atrum. Isolates were screened according to their hydrolytic activities, plant growth-promoting bacteria (PGPB) abilities, as well as for the presence of nonribosomal peptide synthetase (NRPS) genes responsible of the lipopeptide biosynthesis. The results were positive for 16 isolates which exhibited at least two PGPB activities and a single NRPS gene. Genetic diversity of the selected isolates was studied using random amplified polymorphic DNA (RAPD) and repetitive element PCR (REP) tools that showed clustering of strains into three major groups (I, II, and III) (i, ii, and iii), respectively. Clustering was further confirmed by the 16S rDNA sequencing that assigned nine isolates to Bacillus velezensis, four isolates to Bacillus methyltrophicus, one isolate to Bacillus amyloliquefaciens, and two isolates to Bacillus mojavensis. Organ-bacterial genotype interaction as well as positive correlation with NRPS genes are discussed.
DOI: 10.1007/s12010-014-1062-4
PubMed: 25074353
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000301
Links to Exploration step
pubmed:25074353Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Isolation and characterization of putative endophytic bacteria antagonistic to Phoma tracheiphila and Verticillium albo-atrum.</title><author><name sortKey="Kalai Grami, Leila" sort="Kalai Grami, Leila" uniqKey="Kalai Grami L" first="Leila" last="Kalai-Grami">Leila Kalai-Grami</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Biotechnologie Appliquée à l'Agriculture, INRA, Hedi Karray, 2049, Tunis, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratoire de Biotechnologie Appliquée à l'Agriculture, INRA, Hedi Karray, 2049, Tunis</wicri:regionArea></affiliation></author><author><name sortKey="Saidi, Sabrine" sort="Saidi, Sabrine" uniqKey="Saidi S" first="Sabrine" last="Saidi">Sabrine Saidi</name></author><author><name sortKey="Bachkouel, Sarra" sort="Bachkouel, Sarra" uniqKey="Bachkouel S" first="Sarra" last="Bachkouel">Sarra Bachkouel</name></author><author><name sortKey="Ben Slimene, Imen" sort="Ben Slimene, Imen" uniqKey="Ben Slimene I" first="Imen" last="Ben Slimene">Imen Ben Slimene</name></author><author><name sortKey="Mnari Hattab, Monia" sort="Mnari Hattab, Monia" uniqKey="Mnari Hattab M" first="Monia" last="Mnari-Hattab">Monia Mnari-Hattab</name></author><author><name sortKey="Hajlaoui, Mohamed Rebah" sort="Hajlaoui, Mohamed Rebah" uniqKey="Hajlaoui M" first="Mohamed Rebah" last="Hajlaoui">Mohamed Rebah Hajlaoui</name></author><author><name sortKey="Limam, Ferid" sort="Limam, Ferid" uniqKey="Limam F" first="Ferid" last="Limam">Ferid Limam</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:25074353</idno><idno type="pmid">25074353</idno><idno type="doi">10.1007/s12010-014-1062-4</idno><idno type="wicri:Area/PubMed/Corpus">000301</idno><idno type="wicri:Area/PubMed/Curation">000301</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Isolation and characterization of putative endophytic bacteria antagonistic to Phoma tracheiphila and Verticillium albo-atrum.</title><author><name sortKey="Kalai Grami, Leila" sort="Kalai Grami, Leila" uniqKey="Kalai Grami L" first="Leila" last="Kalai-Grami">Leila Kalai-Grami</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Biotechnologie Appliquée à l'Agriculture, INRA, Hedi Karray, 2049, Tunis, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratoire de Biotechnologie Appliquée à l'Agriculture, INRA, Hedi Karray, 2049, Tunis</wicri:regionArea></affiliation></author><author><name sortKey="Saidi, Sabrine" sort="Saidi, Sabrine" uniqKey="Saidi S" first="Sabrine" last="Saidi">Sabrine Saidi</name></author><author><name sortKey="Bachkouel, Sarra" sort="Bachkouel, Sarra" uniqKey="Bachkouel S" first="Sarra" last="Bachkouel">Sarra Bachkouel</name></author><author><name sortKey="Ben Slimene, Imen" sort="Ben Slimene, Imen" uniqKey="Ben Slimene I" first="Imen" last="Ben Slimene">Imen Ben Slimene</name></author><author><name sortKey="Mnari Hattab, Monia" sort="Mnari Hattab, Monia" uniqKey="Mnari Hattab M" first="Monia" last="Mnari-Hattab">Monia Mnari-Hattab</name></author><author><name sortKey="Hajlaoui, Mohamed Rebah" sort="Hajlaoui, Mohamed Rebah" uniqKey="Hajlaoui M" first="Mohamed Rebah" last="Hajlaoui">Mohamed Rebah Hajlaoui</name></author><author><name sortKey="Limam, Ferid" sort="Limam, Ferid" uniqKey="Limam F" first="Ferid" last="Limam">Ferid Limam</name></author></analytic><series><title level="j">Applied biochemistry and biotechnology</title><idno type="eISSN">1559-0291</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ascomycota</term><term>Bacillus (genetics)</term><term>Bacillus (isolation & purification)</term><term>Bacillus (metabolism)</term><term>Bacterial Proteins (genetics)</term><term>Bacterial Proteins (metabolism)</term><term>Lipopeptides (genetics)</term><term>Lipopeptides (metabolism)</term><term>Peptide Synthases (genetics)</term><term>Peptide Synthases (metabolism)</term><term>Plant Diseases</term><term>Random Amplified Polymorphic DNA Technique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>Lipopeptides</term><term>Peptide Synthases</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacillus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacillus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bacillus</term><term>Bacterial Proteins</term><term>Lipopeptides</term><term>Peptide Synthases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Ascomycota</term><term>Plant Diseases</term><term>Random Amplified Polymorphic DNA Technique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A collection of 200 bacterial isolates recovered from citrus plants (Citrus limon, Citrus sinensis, and Citrus reticulata), Medicago truncatula and Laurus nobilis, was established. In vitro screening indicated that 28 isolates exhibited an inhibitory activity against the vascular pathogens Phoma tracheiphila and Verticillium albo-atrum. Isolates were screened according to their hydrolytic activities, plant growth-promoting bacteria (PGPB) abilities, as well as for the presence of nonribosomal peptide synthetase (NRPS) genes responsible of the lipopeptide biosynthesis. The results were positive for 16 isolates which exhibited at least two PGPB activities and a single NRPS gene. Genetic diversity of the selected isolates was studied using random amplified polymorphic DNA (RAPD) and repetitive element PCR (REP) tools that showed clustering of strains into three major groups (I, II, and III) (i, ii, and iii), respectively. Clustering was further confirmed by the 16S rDNA sequencing that assigned nine isolates to Bacillus velezensis, four isolates to Bacillus methyltrophicus, one isolate to Bacillus amyloliquefaciens, and two isolates to Bacillus mojavensis. Organ-bacterial genotype interaction as well as positive correlation with NRPS genes are discussed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25074353</PMID><DateCreated><Year>2014</Year><Month>9</Month><Day>1</Day></DateCreated><DateCompleted><Year>2015</Year><Month>05</Month><Day>12</Day></DateCompleted><DateRevised><Year>2014</Year><Month>9</Month><Day>1</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1559-0291</ISSN><JournalIssue CitedMedium="Internet"><Volume>174</Volume><Issue>1</Issue><PubDate><Year>2014</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Applied biochemistry and biotechnology</Title><ISOAbbreviation>Appl. Biochem. Biotechnol.</ISOAbbreviation></Journal><ArticleTitle>Isolation and characterization of putative endophytic bacteria antagonistic to Phoma tracheiphila and Verticillium albo-atrum.</ArticleTitle><Pagination><MedlinePgn>365-75</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s12010-014-1062-4</ELocationID><Abstract><AbstractText>A collection of 200 bacterial isolates recovered from citrus plants (Citrus limon, Citrus sinensis, and Citrus reticulata), Medicago truncatula and Laurus nobilis, was established. In vitro screening indicated that 28 isolates exhibited an inhibitory activity against the vascular pathogens Phoma tracheiphila and Verticillium albo-atrum. Isolates were screened according to their hydrolytic activities, plant growth-promoting bacteria (PGPB) abilities, as well as for the presence of nonribosomal peptide synthetase (NRPS) genes responsible of the lipopeptide biosynthesis. The results were positive for 16 isolates which exhibited at least two PGPB activities and a single NRPS gene. Genetic diversity of the selected isolates was studied using random amplified polymorphic DNA (RAPD) and repetitive element PCR (REP) tools that showed clustering of strains into three major groups (I, II, and III) (i, ii, and iii), respectively. Clustering was further confirmed by the 16S rDNA sequencing that assigned nine isolates to Bacillus velezensis, four isolates to Bacillus methyltrophicus, one isolate to Bacillus amyloliquefaciens, and two isolates to Bacillus mojavensis. Organ-bacterial genotype interaction as well as positive correlation with NRPS genes are discussed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kalai-Grami</LastName><ForeName>Leila</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Laboratoire de Biotechnologie Appliquée à l'Agriculture, INRA, Hedi Karray, 2049, Tunis, Tunisia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saidi</LastName><ForeName>Sabrine</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Bachkouel</LastName><ForeName>Sarra</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Ben Slimene</LastName><ForeName>Imen</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Mnari-Hattab</LastName><ForeName>Monia</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Hajlaoui</LastName><ForeName>Mohamed Rebah</ForeName><Initials>MR</Initials></Author><Author ValidYN="Y"><LastName>Limam</LastName><ForeName>Ferid</ForeName><Initials>F</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>Jul</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Appl Biochem Biotechnol</MedlineTA><NlmUniqueID>8208561</NlmUniqueID><ISSNLinking>0273-2289</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D055666">Lipopeptides</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 6.3.2.-</RegistryNumber><NameOfSubstance UI="D010453">Peptide Synthases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 6.3.2.-</RegistryNumber><NameOfSubstance UI="C104385">non-ribosomal peptide synthase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="Y">Ascomycota</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001407" MajorTopicYN="Y">Bacillus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="Y">Bacterial Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055666" MajorTopicYN="Y">Lipopeptides</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010453" MajorTopicYN="Y">Peptide Synthases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="Y">Plant Diseases</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019105" MajorTopicYN="N">Random Amplified Polymorphic DNA Technique</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>1</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>7</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>7</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>7</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>5</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25074353</ArticleId><ArticleId IdType="doi">10.1007/s12010-014-1062-4</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/PubMed/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000301 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 000301 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= PubMed
|étape= Curation
|type= RBID
|clé= pubmed:25074353
|texte= Isolation and characterization of putative endophytic bacteria antagonistic to Phoma tracheiphila and Verticillium albo-atrum.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i -Sk "pubmed:25074353" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |