miRNAome analysis associated with anatomic and transcriptomic investigations reveal the polar exhibition of corky split vein in boron deficient Citrus sinensis.
Identifieur interne : 000131 ( PubMed/Corpus ); précédent : 000130; suivant : 000132miRNAome analysis associated with anatomic and transcriptomic investigations reveal the polar exhibition of corky split vein in boron deficient Citrus sinensis.
Auteurs : Chengquan Yang ; Tao Liu ; Fuxi Bai ; Nannan Wang ; Zhiyong Pan ; Xiang Yan ; Shuang PengSource :
- Molecular genetics and genomics : MGG [ 1617-4623 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical , deficiency : Boron.
- genetics : Citrus sinensis, MicroRNAs, RNA, Plant.
- metabolism : Citrus sinensis.
- Sequence Analysis, RNA, Transcriptome.
Abstract
Corky split vein can develop under long-term boron deficient conditions in Citrus sinensis L. Osbeck cv. Newhall. This symptom only occurs in the upper rather than the lower epidermis of old leaves. Our previous study demonstrated that vascular hypertrophy was involved in the symptoms, and the 3rd developmental stage of corky split vein (BD3) was the critical stage for phenotype formation. Here, we performed an intensive study on the BD3 vein and its control sample (CK3 vein). A lignin test demonstrated that the lignin content in BD3 vein was approximately 1.7 times more than the CK3 vein. Anatomical investigation of the corky split vein indicated that the upper epidermis was destroyed by overgrowing vascular cells, and the increased lignin may contribute to vascular cell differentiation and wounding-induced lignification. In a subsequent small RNA sequencing of the BD3 and CK3 veins, 99 known miRNAs and 22 novel miRNAs were identified. Comparative profiling of these miRNAs demonstrated that the 57 known miRNAs and all novel miRNAs exhibited significant expression differences between the two small RNAs libraries of the BD3 and CK3 veins. Associated with our corresponding digital gene expression data, we propose that the decreased expression of two miRNAs, csi-miR156b and csi-miR164, which leads to the up-regulation of their target genes, SPLs (csi-miR156b-targeted) and CUC2 (csi-miR164-targeted), may promote vascular cell division and orderless stage transition in old leaves.
DOI: 10.1007/s00438-015-1024-8
PubMed: 25754997
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Huazhong Agricultural University, Wuhan, 430070, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Nannan" sort="Wang, Nannan" uniqKey="Wang N" first="Nannan" last="Wang">Nannan Wang</name><affiliation><nlm:affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.</nlm:affiliation></affiliation></author><author><name sortKey="Pan, Zhiyong" sort="Pan, Zhiyong" uniqKey="Pan Z" first="Zhiyong" last="Pan">Zhiyong Pan</name><affiliation><nlm:affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yan, Xiang" sort="Yan, Xiang" uniqKey="Yan X" first="Xiang" last="Yan">Xiang Yan</name><affiliation><nlm:affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.</nlm:affiliation></affiliation></author><author><name sortKey="Peng, Shuang" sort="Peng, Shuang" uniqKey="Peng S" first="Shuang" last="Peng">Shuang Peng</name><affiliation><nlm:affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China. shuangpeng428@126.com.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25754997</idno><idno type="pmid">25754997</idno><idno type="doi">10.1007/s00438-015-1024-8</idno><idno type="wicri:Area/PubMed/Corpus">000131</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">miRNAome analysis associated with anatomic and transcriptomic investigations reveal the polar exhibition of corky split vein in boron deficient Citrus sinensis.</title><author><name sortKey="Yang, Chengquan" sort="Yang, Chengquan" uniqKey="Yang C" first="Chengquan" last="Yang">Chengquan Yang</name><affiliation><nlm:affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Tao" sort="Liu, Tao" uniqKey="Liu T" first="Tao" last="Liu">Tao Liu</name><affiliation><nlm:affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.</nlm:affiliation></affiliation></author><author><name sortKey="Bai, Fuxi" sort="Bai, Fuxi" uniqKey="Bai F" first="Fuxi" last="Bai">Fuxi Bai</name><affiliation><nlm:affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Nannan" sort="Wang, Nannan" uniqKey="Wang N" first="Nannan" last="Wang">Nannan Wang</name><affiliation><nlm:affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.</nlm:affiliation></affiliation></author><author><name sortKey="Pan, Zhiyong" sort="Pan, Zhiyong" uniqKey="Pan Z" first="Zhiyong" last="Pan">Zhiyong Pan</name><affiliation><nlm:affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yan, Xiang" sort="Yan, Xiang" uniqKey="Yan X" first="Xiang" last="Yan">Xiang Yan</name><affiliation><nlm:affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.</nlm:affiliation></affiliation></author><author><name sortKey="Peng, Shuang" sort="Peng, Shuang" uniqKey="Peng S" first="Shuang" last="Peng">Shuang Peng</name><affiliation><nlm:affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China. shuangpeng428@126.com.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Molecular genetics and genomics : MGG</title><idno type="eISSN">1617-4623</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Boron (deficiency)</term><term>Citrus sinensis (genetics)</term><term>Citrus sinensis (metabolism)</term><term>MicroRNAs (genetics)</term><term>RNA, Plant (genetics)</term><term>Sequence Analysis, RNA</term><term>Transcriptome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="deficiency" xml:lang="en"><term>Boron</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus sinensis</term><term>MicroRNAs</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Sequence Analysis, RNA</term><term>Transcriptome</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Corky split vein can develop under long-term boron deficient conditions in Citrus sinensis L. Osbeck cv. Newhall. This symptom only occurs in the upper rather than the lower epidermis of old leaves. Our previous study demonstrated that vascular hypertrophy was involved in the symptoms, and the 3rd developmental stage of corky split vein (BD3) was the critical stage for phenotype formation. Here, we performed an intensive study on the BD3 vein and its control sample (CK3 vein). A lignin test demonstrated that the lignin content in BD3 vein was approximately 1.7 times more than the CK3 vein. Anatomical investigation of the corky split vein indicated that the upper epidermis was destroyed by overgrowing vascular cells, and the increased lignin may contribute to vascular cell differentiation and wounding-induced lignification. In a subsequent small RNA sequencing of the BD3 and CK3 veins, 99 known miRNAs and 22 novel miRNAs were identified. Comparative profiling of these miRNAs demonstrated that the 57 known miRNAs and all novel miRNAs exhibited significant expression differences between the two small RNAs libraries of the BD3 and CK3 veins. Associated with our corresponding digital gene expression data, we propose that the decreased expression of two miRNAs, csi-miR156b and csi-miR164, which leads to the up-regulation of their target genes, SPLs (csi-miR156b-targeted) and CUC2 (csi-miR164-targeted), may promote vascular cell division and orderless stage transition in old leaves.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25754997</PMID><DateCreated><Year>2015</Year><Month>9</Month><Day>16</Day></DateCreated><DateCompleted><Year>2015</Year><Month>12</Month><Day>07</Day></DateCompleted><DateRevised><Year>2015</Year><Month>9</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1617-4623</ISSN><JournalIssue CitedMedium="Internet"><Volume>290</Volume><Issue>5</Issue><PubDate><Year>2015</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Molecular genetics and genomics : MGG</Title><ISOAbbreviation>Mol. Genet. Genomics</ISOAbbreviation></Journal><ArticleTitle>miRNAome analysis associated with anatomic and transcriptomic investigations reveal the polar exhibition of corky split vein in boron deficient Citrus sinensis.</ArticleTitle><Pagination><MedlinePgn>1639-57</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00438-015-1024-8</ELocationID><Abstract><AbstractText>Corky split vein can develop under long-term boron deficient conditions in Citrus sinensis L. Osbeck cv. Newhall. This symptom only occurs in the upper rather than the lower epidermis of old leaves. Our previous study demonstrated that vascular hypertrophy was involved in the symptoms, and the 3rd developmental stage of corky split vein (BD3) was the critical stage for phenotype formation. Here, we performed an intensive study on the BD3 vein and its control sample (CK3 vein). A lignin test demonstrated that the lignin content in BD3 vein was approximately 1.7 times more than the CK3 vein. Anatomical investigation of the corky split vein indicated that the upper epidermis was destroyed by overgrowing vascular cells, and the increased lignin may contribute to vascular cell differentiation and wounding-induced lignification. In a subsequent small RNA sequencing of the BD3 and CK3 veins, 99 known miRNAs and 22 novel miRNAs were identified. Comparative profiling of these miRNAs demonstrated that the 57 known miRNAs and all novel miRNAs exhibited significant expression differences between the two small RNAs libraries of the BD3 and CK3 veins. Associated with our corresponding digital gene expression data, we propose that the decreased expression of two miRNAs, csi-miR156b and csi-miR164, which leads to the up-regulation of their target genes, SPLs (csi-miR156b-targeted) and CUC2 (csi-miR164-targeted), may promote vascular cell division and orderless stage transition in old leaves.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Chengquan</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, Wuhan, 430070, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, Wuhan, 430070, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Tao</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, Wuhan, 430070, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, Wuhan, 430070, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bai</LastName><ForeName>Fuxi</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, Wuhan, 430070, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, Wuhan, 430070, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Nannan</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, Wuhan, 430070, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, Wuhan, 430070, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pan</LastName><ForeName>Zhiyong</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, Wuhan, 430070, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, Wuhan, 430070, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Xiang</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, Wuhan, 430070, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, Wuhan, 430070, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Peng</LastName><ForeName>ShuAng</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China. shuangpeng428@126.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology, Ministry of Education, Wuhan, 430070, China. shuangpeng428@126.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, Wuhan, 430070, China. shuangpeng428@126.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>Mar</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Mol Genet Genomics</MedlineTA><NlmUniqueID>101093320</NlmUniqueID><ISSNLinking>1617-4623</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D035683">MicroRNAs</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>N9E3X5056Q</RegistryNumber><NameOfSubstance UI="D001895">Boron</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001895" MajorTopicYN="N">Boron</DescriptorName><QualifierName UI="Q000172" MajorTopicYN="Y">deficiency</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D035683" MajorTopicYN="N">MicroRNAs</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017423" MajorTopicYN="N">Sequence Analysis, RNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="Y">Transcriptome</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Anatomy</Keyword><Keyword MajorTopicYN="N">Cell division</Keyword><Keyword MajorTopicYN="N">Corky split vein</Keyword><Keyword MajorTopicYN="N">Lignification</Keyword><Keyword MajorTopicYN="N">Orderless stage transition</Keyword><Keyword MajorTopicYN="N">miRNAome</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>12</Month><Day>8</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>3</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>3</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>3</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25754997</ArticleId><ArticleId IdType="doi">10.1007/s00438-015-1024-8</ArticleId><ArticleId IdType="pii">10.1007/s00438-015-1024-8</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix 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