A R2R3-MYB Transcription Factor from Epimedium sagittatum Regulates the Flavonoid Biosynthetic Pathway
Identifieur interne : 001154 ( Pmc/Curation ); précédent : 001153; suivant : 001155A R2R3-MYB Transcription Factor from Epimedium sagittatum Regulates the Flavonoid Biosynthetic Pathway
Auteurs : Wenjun Huang [République populaire de Chine] ; Wei Sun [République populaire de Chine] ; Haiyan Lv [République populaire de Chine] ; Ming Luo [République populaire de Chine] ; Shaohua Zeng [République populaire de Chine] ; Sitakanta Pattanaik [États-Unis] ; Ling Yuan [États-Unis] ; Ying Wang [République populaire de Chine]Source :
- PLoS ONE [ 1932-6203 ] ; 2013.
Abstract
Herba epimedii (
Url:
DOI: 10.1371/journal.pone.0070778
PubMed: 23936468
PubMed Central: 3731294
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Regulates the Flavonoid Biosynthetic Pathway</title>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A R2R3-MYB Transcription Factor from <italic>Epimedium sagittatum</italic>
Regulates the Flavonoid Biosynthetic Pathway</title>
<author><name sortKey="Huang, Wenjun" sort="Huang, Wenjun" uniqKey="Huang W" first="Wenjun" last="Huang">Wenjun Huang</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Sun, Wei" sort="Sun, Wei" uniqKey="Sun W" first="Wei" last="Sun">Wei Sun</name>
<affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Lv, Haiyan" sort="Lv, Haiyan" uniqKey="Lv H" first="Haiyan" last="Lv">Haiyan Lv</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Luo, Ming" sort="Luo, Ming" uniqKey="Luo M" first="Ming" last="Luo">Ming Luo</name>
<affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zeng, Shaohua" sort="Zeng, Shaohua" uniqKey="Zeng S" first="Shaohua" last="Zeng">Shaohua Zeng</name>
<affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, China</addr-line>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Pattanaik, Sitakanta" sort="Pattanaik, Sitakanta" uniqKey="Pattanaik S" first="Sitakanta" last="Pattanaik">Sitakanta Pattanaik</name>
<affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yuan, Ling" sort="Yuan, Ling" uniqKey="Yuan L" first="Ling" last="Yuan">Ling Yuan</name>
<affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Wang, Ying" sort="Wang, Ying" uniqKey="Wang Y" first="Ying" last="Wang">Ying Wang</name>
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</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei</wicri:regionArea>
</affiliation>
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<front><div type="abstract" xml:lang="en"><p>Herba epimedii (<italic>Epimedium</italic>
), a traditional Chinese medicine, has been widely used as a kidney tonic and antirheumatic medicine for thousands of years. The bioactive components in herba epimedii are mainly prenylated flavonol glycosides, end-products of the flavonoid pathway. <italic>Epimedium</italic>
species are also used as garden plants due to the colorful flowers and leaves. Many R2R3-MYB transcription factors (TFs) have been identified to regulate the flavonoid and anthocyanin biosynthetic pathways. However, little is known about the R2R3-MYB TFs involved in regulation of the flavonoid pathway in <italic>Epimedium</italic>
. Here, we reported the isolation and functional characterization of the first <italic>R2R3-MYB</italic>
TF (<italic>EsMYBA1</italic>
) from <italic>Epimedium sagittatum</italic>
(Sieb. Et Zucc.) Maxim. Conserved domains and phylogenetic analysis showed that EsMYBA1 belonged to the subgroup 6 clade (anthocyanin-related MYB clade) of R2R3-MYB family, which includes <italic>Arabidopsis</italic>
AtPAP1, apple MdMYB10 and legume MtLAP1. <italic>EsMYBA1</italic>
was preferentially expressed in leaves, especially in red leaves that contain higher content of anthocyanin. Alternative splicing of <italic>EsMYBA1</italic>
resulted in three transcripts and two of them encoded a MYB-related protein. Yeast two-hybrid and transient luciferase expression assay showed that EsMYBA1 can interact with several bHLH regulators of the flavonoid pathway and activate the promoters of dihydroflavonol 4-reductase (<italic>DFR</italic>
) and anthocyanidin synthase (<italic>ANS</italic>
). In both transgenic tobacco and <italic>Arabidopsis</italic>
, overexpression of <italic>EsMYBA1</italic>
induced strong anthocyanin accumulation in reproductive and/or vegetative tissues via up-regulation of the main flavonoid-related genes. Furthermore, transient expression of <italic>EsMYBA1</italic>
in <italic>E. sagittatum</italic>
leaves by <italic>Agrobacterium</italic>
infiltration also induced anthocyanin accumulation in the wounded area. This first functional characterization of R2R3-MYB TFs in <italic>Epimedium</italic>
species will promote further studies of the flavonoid biosynthesis and regulation in medicinal plants.</p>
</div>
</front>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id>
<journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id>
<journal-id journal-id-type="publisher-id">plos</journal-id>
<journal-id journal-id-type="pmc">plosone</journal-id>
<journal-title-group><journal-title>PLoS ONE</journal-title>
</journal-title-group>
<issn pub-type="epub">1932-6203</issn>
<publisher><publisher-name>Public Library of Science</publisher-name>
<publisher-loc>San Francisco, USA</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">23936468</article-id>
<article-id pub-id-type="pmc">3731294</article-id>
<article-id pub-id-type="publisher-id">PONE-D-13-13580</article-id>
<article-id pub-id-type="doi">10.1371/journal.pone.0070778</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
<subj-group subj-group-type="Discipline-v2"><subject>Biology</subject>
<subj-group><subject>Biochemistry</subject>
<subj-group><subject>Metabolism</subject>
<subj-group><subject>Biosynthesis</subject>
</subj-group>
</subj-group>
<subj-group><subject>Plant Biochemistry</subject>
<subj-group><subject>Secondary Metabolism</subject>
</subj-group>
</subj-group>
<subj-group><subject>Proteins</subject>
<subj-group><subject>Protein Interactions</subject>
</subj-group>
</subj-group>
</subj-group>
<subj-group><subject>Evolutionary Biology</subject>
<subj-group><subject>Evolutionary Systematics</subject>
<subj-group><subject>Phylogenetics</subject>
</subj-group>
</subj-group>
</subj-group>
<subj-group><subject>Plant Science</subject>
<subj-group><subject>Plant Biochemistry</subject>
<subj-group><subject>Secondary Metabolism</subject>
</subj-group>
</subj-group>
<subj-group><subject>Plant Physiology</subject>
</subj-group>
</subj-group>
<subj-group><subject>Proteomics</subject>
<subj-group><subject>Protein Interactions</subject>
</subj-group>
</subj-group>
</subj-group>
<subj-group subj-group-type="Discipline-v2"><subject>Medicine</subject>
<subj-group><subject>Complementary and Alternative Medicine</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group><article-title>A R2R3-MYB Transcription Factor from <italic>Epimedium sagittatum</italic>
Regulates the Flavonoid Biosynthetic Pathway</article-title>
<alt-title alt-title-type="running-head">Epimedium MYBA1 Regulates the Flavonoid Pathway</alt-title>
</title-group>
<contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Huang</surname>
<given-names>Wenjun</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author" equal-contrib="yes"><name><surname>Sun</surname>
<given-names>Wei</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Lv</surname>
<given-names>Haiyan</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Luo</surname>
<given-names>Ming</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zeng</surname>
<given-names>Shaohua</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Pattanaik</surname>
<given-names>Sitakanta</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Yuan</surname>
<given-names>Ling</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Ying</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="corresp" rid="cor1"><sup>*</sup>
</xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>1</label>
<addr-line>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China</addr-line>
</aff>
<aff id="aff2"><label>2</label>
<addr-line>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, China</addr-line>
</aff>
<aff id="aff3"><label>3</label>
<addr-line>Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky, United States of America</addr-line>
</aff>
<contrib-group><contrib contrib-type="editor"><name><surname>Schiefelbein</surname>
<given-names>John</given-names>
</name>
<role>Editor</role>
<xref ref-type="aff" rid="edit1"></xref>
</contrib>
</contrib-group>
<aff id="edit1"><addr-line>University of Michigan, United States of America</addr-line>
</aff>
<author-notes><corresp id="cor1">* E-mail: <email>yingwang@wbgcas.cn</email>
</corresp>
<fn fn-type="conflict"><p><bold>Competing Interests: </bold>
The authors have declared that no competing interests exist.</p>
</fn>
<fn fn-type="con"><p>Conceived and designed the experiments: YW. Performed the experiments: WH WS ML SP. Analyzed the data: WH WS LY. Contributed reagents/materials/analysis tools: HL SZ. Wrote the paper: WH YW.</p>
</fn>
</author-notes>
<pub-date pub-type="collection"><year>2013</year>
</pub-date>
<pub-date pub-type="epub"><day>1</day>
<month>8</month>
<year>2013</year>
</pub-date>
<volume>8</volume>
<issue>8</issue>
<elocation-id>e70778</elocation-id>
<history><date date-type="received"><day>26</day>
<month>3</month>
<year>2013</year>
</date>
<date date-type="accepted"><day>21</day>
<month>6</month>
<year>2013</year>
</date>
</history>
<permissions><copyright-year>2013</copyright-year>
<copyright-holder>Huang et al</copyright-holder>
<license><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p>
</license>
</permissions>
<abstract><p>Herba epimedii (<italic>Epimedium</italic>
), a traditional Chinese medicine, has been widely used as a kidney tonic and antirheumatic medicine for thousands of years. The bioactive components in herba epimedii are mainly prenylated flavonol glycosides, end-products of the flavonoid pathway. <italic>Epimedium</italic>
species are also used as garden plants due to the colorful flowers and leaves. Many R2R3-MYB transcription factors (TFs) have been identified to regulate the flavonoid and anthocyanin biosynthetic pathways. However, little is known about the R2R3-MYB TFs involved in regulation of the flavonoid pathway in <italic>Epimedium</italic>
. Here, we reported the isolation and functional characterization of the first <italic>R2R3-MYB</italic>
TF (<italic>EsMYBA1</italic>
) from <italic>Epimedium sagittatum</italic>
(Sieb. Et Zucc.) Maxim. Conserved domains and phylogenetic analysis showed that EsMYBA1 belonged to the subgroup 6 clade (anthocyanin-related MYB clade) of R2R3-MYB family, which includes <italic>Arabidopsis</italic>
AtPAP1, apple MdMYB10 and legume MtLAP1. <italic>EsMYBA1</italic>
was preferentially expressed in leaves, especially in red leaves that contain higher content of anthocyanin. Alternative splicing of <italic>EsMYBA1</italic>
resulted in three transcripts and two of them encoded a MYB-related protein. Yeast two-hybrid and transient luciferase expression assay showed that EsMYBA1 can interact with several bHLH regulators of the flavonoid pathway and activate the promoters of dihydroflavonol 4-reductase (<italic>DFR</italic>
) and anthocyanidin synthase (<italic>ANS</italic>
). In both transgenic tobacco and <italic>Arabidopsis</italic>
, overexpression of <italic>EsMYBA1</italic>
induced strong anthocyanin accumulation in reproductive and/or vegetative tissues via up-regulation of the main flavonoid-related genes. Furthermore, transient expression of <italic>EsMYBA1</italic>
in <italic>E. sagittatum</italic>
leaves by <italic>Agrobacterium</italic>
infiltration also induced anthocyanin accumulation in the wounded area. This first functional characterization of R2R3-MYB TFs in <italic>Epimedium</italic>
species will promote further studies of the flavonoid biosynthesis and regulation in medicinal plants.</p>
</abstract>
<funding-group><funding-statement>This study was supported by grants from the National Natural Science Foundation of China (No. 31270340, 31200225), and CAS/SAFEA International Partnership Program for Creative Research Teams Project and Knowledge Innovation Project of The Chinese Academy of Sciences (KSCX2-EW-J-20). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement>
</funding-group>
<counts><page-count count="16"></page-count>
</counts>
</article-meta>
</front>
</pmc>
</record>
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