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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Altering the Expression of the Chlorophyllase Gene <italic>ATHCOR1</italic>
in Transgenic Arabidopsis Caused Changes in the Chlorophyll-to-Chlorophyllide Ratio<xref ref-type="fn" rid="FN1">1</xref>
</title>
<author><name sortKey="Benedetti, Celso Eduardo" sort="Benedetti, Celso Eduardo" uniqKey="Benedetti C" first="Celso Eduardo" last="Benedetti">Celso Eduardo Benedetti</name>
<affiliation><nlm:aff id="N0x9e06d20N0x9ebfb30">Centro de Biologia Molecular Estrutural, Laboratório Nacional de Luz Síncrotron, Campinas, SP, CP6192, CEP 13084–971, Brazil (C.E.B.); and Centro de Biologia Molecular e Engenharia Genética, and Depto de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, CP6010, CEP 13083–970, Campinas, SP, Brazil (P.A.)</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Arruda, Paulo" sort="Arruda, Paulo" uniqKey="Arruda P" first="Paulo" last="Arruda">Paulo Arruda</name>
<affiliation><nlm:aff id="N0x9e06d20N0x9ebfb30">Centro de Biologia Molecular Estrutural, Laboratório Nacional de Luz Síncrotron, Campinas, SP, CP6192, CEP 13084–971, Brazil (C.E.B.); and Centro de Biologia Molecular e Engenharia Genética, and Depto de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, CP6010, CEP 13083–970, Campinas, SP, Brazil (P.A.)</nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">11950974</idno>
<idno type="pmc">154253</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC154253</idno>
<idno type="RBID">PMC:154253</idno>
<idno type="doi">10.1104/pp.010813</idno>
<date when="2002">2002</date>
<idno type="wicri:Area/Pmc/Corpus">000538</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Altering the Expression of the Chlorophyllase Gene <italic>ATHCOR1</italic>
in Transgenic Arabidopsis Caused Changes in the Chlorophyll-to-Chlorophyllide Ratio<xref ref-type="fn" rid="FN1">1</xref>
</title>
<author><name sortKey="Benedetti, Celso Eduardo" sort="Benedetti, Celso Eduardo" uniqKey="Benedetti C" first="Celso Eduardo" last="Benedetti">Celso Eduardo Benedetti</name>
<affiliation><nlm:aff id="N0x9e06d20N0x9ebfb30">Centro de Biologia Molecular Estrutural, Laboratório Nacional de Luz Síncrotron, Campinas, SP, CP6192, CEP 13084–971, Brazil (C.E.B.); and Centro de Biologia Molecular e Engenharia Genética, and Depto de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, CP6010, CEP 13083–970, Campinas, SP, Brazil (P.A.)</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Arruda, Paulo" sort="Arruda, Paulo" uniqKey="Arruda P" first="Paulo" last="Arruda">Paulo Arruda</name>
<affiliation><nlm:aff id="N0x9e06d20N0x9ebfb30">Centro de Biologia Molecular Estrutural, Laboratório Nacional de Luz Síncrotron, Campinas, SP, CP6192, CEP 13084–971, Brazil (C.E.B.); and Centro de Biologia Molecular e Engenharia Genética, and Depto de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, CP6010, CEP 13083–970, Campinas, SP, Brazil (P.A.)</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Plant Physiology</title>
<idno type="ISSN">0032-0889</idno>
<idno type="eISSN">1532-2548</idno>
<imprint><date when="2002">2002</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The Arabidopsis gene <italic>ATHCOR1</italic>
, which encodes the CORI1 (coronatine-induced) protein, was expressed in bacterial cells. Soluble recombinant CORI1 was purified and shown to possess chlorophyllase (Chlase) activity in vitro. To determine its activity in vivo, wild-type Arabidopsis and <italic>coi1</italic>
mutant, which lacks <italic>ATHCOR1</italic>
transcripts, were transformed with sense and antisense forms of the gene. Wild-type and <italic>coi1</italic>
plants overexpressing <italic>ATHCOR1</italic>
showed increased contents of chlorophyllide (Chlide) without a substantial change in the total amount of the extractable chlorophyll (Chl). These plants presented high Chlide to Chl ratios in leaves, whereas antisense plants and nontransformed <italic>coi1</italic>
mutant showed undetectable <italic>ATHCOR1</italic>
mRNA and significantly lower Chlide to Chl ratios, relative to wild-type control. Overexpression of <italic>ATHCOR1</italic>
caused an increased breakdown of Chl <italic>a</italic>
, as revealed by the Chlide <italic>a</italic>
to <italic>b</italic>
ratio, which was significantly higher in sense than wild-type, <italic>coi1</italic>
mutant, and antisense plants. This preferential activity of CORI1 toward Chl <italic>a</italic>
was further supported by in vitro analyses using the purified protein. Increased Chlase activity was detected in developing flowers, which correlated to the constitutive expression of <italic>ATHCOR1</italic>
in this organ. Flowers of the antisense plant showed reduced Chlide to Chl ratio, suggesting a role of CORI1 in Chl breakdown during flower senescence. The results show that <italic>ATHCOR1</italic>
has Chlase activity in vivo, however, because <italic>coi1</italic>
flowers have no detectable <italic>ATHCOR1</italic>
mRNA and present Chlide to Chl ratios comparable with the wild type, an additional Chlase is likely to be active in Arabidopsis. In accordance, transcripts of a second Arabidopsis Chlase gene, <italic>AtCLH2</italic>
, were detected in both normal and mutant flowers.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id>
<journal-id journal-id-type="publisher-id">PLANT PHYSIOL</journal-id>
<journal-title>Plant Physiology</journal-title>
<issn pub-type="ppub">0032-0889</issn>
<issn pub-type="epub">1532-2548</issn>
<publisher><publisher-name>American Society of Plant Physiologists</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">11950974</article-id>
<article-id pub-id-type="pmc">154253</article-id>
<article-id pub-id-type="publisher-id">7177</article-id>
<article-id pub-id-type="doi">10.1104/pp.010813</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
<subj-group><subject>Biochemical Processes and Macromolecular Structures</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group><article-title>Altering the Expression of the Chlorophyllase Gene <italic>ATHCOR1</italic>
in Transgenic Arabidopsis Caused Changes in the Chlorophyll-to-Chlorophyllide Ratio<xref ref-type="fn" rid="FN1">1</xref>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Benedetti</surname>
<given-names>Celso Eduardo</given-names>
</name>
<xref ref-type="author-notes" rid="FN150">*</xref>
<xref ref-type="aff" rid="N0x9e06d20N0x9ebfb30"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Arruda</surname>
<given-names>Paulo</given-names>
</name>
<xref ref-type="aff" rid="N0x9e06d20N0x9ebfb30"></xref>
</contrib>
</contrib-group>
<aff id="N0x9e06d20N0x9ebfb30">Centro de Biologia Molecular Estrutural, Laboratório Nacional de Luz Síncrotron, Campinas, SP, CP6192, CEP 13084–971, Brazil (C.E.B.); and Centro de Biologia Molecular e Engenharia Genética, and Depto de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, CP6010, CEP 13083–970, Campinas, SP, Brazil (P.A.)</aff>
<author-notes><fn id="FN150"><label>*</label>
<p>Corresponding author; e-mail <email>celso@lnls.br</email>
; fax 5519–32877110.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>4</month>
<year>2002</year>
</pub-date>
<volume>128</volume>
<issue>4</issue>
<fpage>1255</fpage>
<lpage>1263</lpage>
<history><date date-type="received"><day>4</day>
<month>9</month>
<year>2001</year>
</date>
<date date-type="rev-recd"><day>31</day>
<month>10</month>
<year>2001</year>
</date>
<date date-type="accepted"><day>4</day>
<month>1</month>
<year>2002</year>
</date>
</history>
<copyright-statement>Copyright © 2002, American Society of Plant Physiologists</copyright-statement>
<copyright-year>2002</copyright-year>
<abstract><p>The Arabidopsis gene <italic>ATHCOR1</italic>
, which encodes the CORI1 (coronatine-induced) protein, was expressed in bacterial cells. Soluble recombinant CORI1 was purified and shown to possess chlorophyllase (Chlase) activity in vitro. To determine its activity in vivo, wild-type Arabidopsis and <italic>coi1</italic>
mutant, which lacks <italic>ATHCOR1</italic>
transcripts, were transformed with sense and antisense forms of the gene. Wild-type and <italic>coi1</italic>
plants overexpressing <italic>ATHCOR1</italic>
showed increased contents of chlorophyllide (Chlide) without a substantial change in the total amount of the extractable chlorophyll (Chl). These plants presented high Chlide to Chl ratios in leaves, whereas antisense plants and nontransformed <italic>coi1</italic>
mutant showed undetectable <italic>ATHCOR1</italic>
mRNA and significantly lower Chlide to Chl ratios, relative to wild-type control. Overexpression of <italic>ATHCOR1</italic>
caused an increased breakdown of Chl <italic>a</italic>
, as revealed by the Chlide <italic>a</italic>
to <italic>b</italic>
ratio, which was significantly higher in sense than wild-type, <italic>coi1</italic>
mutant, and antisense plants. This preferential activity of CORI1 toward Chl <italic>a</italic>
was further supported by in vitro analyses using the purified protein. Increased Chlase activity was detected in developing flowers, which correlated to the constitutive expression of <italic>ATHCOR1</italic>
in this organ. Flowers of the antisense plant showed reduced Chlide to Chl ratio, suggesting a role of CORI1 in Chl breakdown during flower senescence. The results show that <italic>ATHCOR1</italic>
has Chlase activity in vivo, however, because <italic>coi1</italic>
flowers have no detectable <italic>ATHCOR1</italic>
mRNA and present Chlide to Chl ratios comparable with the wild type, an additional Chlase is likely to be active in Arabidopsis. In accordance, transcripts of a second Arabidopsis Chlase gene, <italic>AtCLH2</italic>
, were detected in both normal and mutant flowers.</p>
</abstract>
</article-meta>
</front>
</pmc>
</record>
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