Microbes Associated with Freshly Prepared Juices of Citrus and Carrots
Identifieur interne : 000342 ( Pmc/Corpus ); précédent : 000341; suivant : 000343Microbes Associated with Freshly Prepared Juices of Citrus and Carrots
Auteurs : Kamal Rai Aneja ; Romika Dhiman ; Neeraj Kumar Aggarwal ; Vikas Kumar ; Manpreeet KaurSource :
- International Journal of Food Science [ 2314-5765 ] ; 2014.
Abstract
Fruit juices are popular drinks as they contain antioxidants, vitamins, and minerals that are essential for human being and play important role in the prevention of heart diseases, cancer, and diabetes. They contain essential nutrients which support the growth of acid tolerant bacteria, yeasts, and moulds. In the present study, we have conducted a microbiological examination of freshly prepared juices (sweet lime, orange, and carrot) by serial dilution agar plate technique. A total of 30 juice samples were examined for their microbiological quality. Twenty-five microbial species including 9 bacterial isolates, 5 yeast isolates, and 11 mould isolates were isolated from juices. Yeasts and moulds were the main cause of spoilage of juices.
Url:
DOI: 10.1155/2014/408085
PubMed: 26904628
PubMed Central: 4745523
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Microbes Associated with Freshly Prepared Juices of Citrus and Carrots</title><author><name sortKey="Aneja, Kamal Rai" sort="Aneja, Kamal Rai" uniqKey="Aneja K" first="Kamal Rai" last="Aneja">Kamal Rai Aneja</name><affiliation><nlm:aff id="I1">Vaidyanath Research, Training and Diagnostic Centre, Kurukshetra 136118, India</nlm:aff></affiliation></author><author><name sortKey="Dhiman, Romika" sort="Dhiman, Romika" uniqKey="Dhiman R" first="Romika" last="Dhiman">Romika Dhiman</name><affiliation><nlm:aff id="I2">Department of Microbiology, Kurukshetra University, Kurukshetra 136119, India</nlm:aff></affiliation></author><author><name sortKey="Aggarwal, Neeraj Kumar" sort="Aggarwal, Neeraj Kumar" uniqKey="Aggarwal N" first="Neeraj Kumar" last="Aggarwal">Neeraj Kumar Aggarwal</name><affiliation><nlm:aff id="I2">Department of Microbiology, Kurukshetra University, Kurukshetra 136119, India</nlm:aff></affiliation></author><author><name sortKey="Kumar, Vikas" sort="Kumar, Vikas" uniqKey="Kumar V" first="Vikas" last="Kumar">Vikas Kumar</name><affiliation><nlm:aff id="I2">Department of Microbiology, Kurukshetra University, Kurukshetra 136119, India</nlm:aff></affiliation></author><author><name sortKey="Kaur, Manpreeet" sort="Kaur, Manpreeet" uniqKey="Kaur M" first="Manpreeet" last="Kaur">Manpreeet Kaur</name><affiliation><nlm:aff id="I2">Department of Microbiology, Kurukshetra University, Kurukshetra 136119, India</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26904628</idno><idno type="pmc">4745523</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745523</idno><idno type="RBID">PMC:4745523</idno><idno type="doi">10.1155/2014/408085</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000342</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Microbes Associated with Freshly Prepared Juices of Citrus and Carrots</title><author><name sortKey="Aneja, Kamal Rai" sort="Aneja, Kamal Rai" uniqKey="Aneja K" first="Kamal Rai" last="Aneja">Kamal Rai Aneja</name><affiliation><nlm:aff id="I1">Vaidyanath Research, Training and Diagnostic Centre, Kurukshetra 136118, India</nlm:aff></affiliation></author><author><name sortKey="Dhiman, Romika" sort="Dhiman, Romika" uniqKey="Dhiman R" first="Romika" last="Dhiman">Romika Dhiman</name><affiliation><nlm:aff id="I2">Department of Microbiology, Kurukshetra University, Kurukshetra 136119, India</nlm:aff></affiliation></author><author><name sortKey="Aggarwal, Neeraj Kumar" sort="Aggarwal, Neeraj Kumar" uniqKey="Aggarwal N" first="Neeraj Kumar" last="Aggarwal">Neeraj Kumar Aggarwal</name><affiliation><nlm:aff id="I2">Department of Microbiology, Kurukshetra University, Kurukshetra 136119, India</nlm:aff></affiliation></author><author><name sortKey="Kumar, Vikas" sort="Kumar, Vikas" uniqKey="Kumar V" first="Vikas" last="Kumar">Vikas Kumar</name><affiliation><nlm:aff id="I2">Department of Microbiology, Kurukshetra University, Kurukshetra 136119, India</nlm:aff></affiliation></author><author><name sortKey="Kaur, Manpreeet" sort="Kaur, Manpreeet" uniqKey="Kaur M" first="Manpreeet" last="Kaur">Manpreeet Kaur</name><affiliation><nlm:aff id="I2">Department of Microbiology, Kurukshetra University, Kurukshetra 136119, India</nlm:aff></affiliation></author></analytic><series><title level="j">International Journal of Food Science</title><idno type="eISSN">2314-5765</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Fruit juices are popular drinks as they contain antioxidants, vitamins, and minerals that are essential for human being and play important role in the prevention of heart diseases, cancer, and diabetes. They contain essential nutrients which support the growth of acid tolerant bacteria, yeasts, and moulds. In the present study, we have conducted a microbiological examination of freshly prepared juices (sweet lime, orange, and carrot) by serial dilution agar plate technique. A total of 30 juice samples were examined for their microbiological quality. Twenty-five microbial species including 9 bacterial isolates, 5 yeast isolates, and 11 mould isolates were isolated from juices. Yeasts and moulds were the main cause of spoilage of juices. <italic>Aspergillus flavus</italic> and <italic>Rhodotorula mucilaginosa</italic> were observed in the maximum number of juice samples. Among bacteria <italic>Bacillus cereus</italic> and <italic>Serratia</italic> were dominant. <italic>Escherichia coli</italic> and <italic>Staphylococcus aureus</italic> were detected in few samples. <italic>Candida</italic> sp., <italic>Curvularia</italic>, <italic>Colletotrichum</italic>, and <italic>Acetobacter</italic> were observed only in citrus juice samples. <italic>Alternaria</italic>, <italic>Aspergillus terreus</italic>, <italic>A. niger</italic>, <italic>Cladosporium</italic>, and <italic>Fusarium</italic> were also observed in tested juice samples. Some of the microorganisms detected in these juice samples can cause disease in human beings, so there is need for some guidelines that can improve the quality of fruit juices.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Raybaudi Massilia, R M" uniqKey="Raybaudi Massilia R">R. M. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Int J Food Sci</journal-id><journal-id journal-id-type="iso-abbrev">Int J Food Sci</journal-id><journal-id journal-id-type="publisher-id">IJFS</journal-id><journal-title-group><journal-title>International Journal of Food Science</journal-title></journal-title-group><issn pub-type="epub">2314-5765</issn><publisher><publisher-name>Hindawi Publishing Corporation</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26904628</article-id><article-id pub-id-type="pmc">4745523</article-id><article-id pub-id-type="doi">10.1155/2014/408085</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Microbes Associated with Freshly Prepared Juices of Citrus and Carrots</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Aneja</surname><given-names>Kamal Rai</given-names></name><xref ref-type="aff" rid="I1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Dhiman</surname><given-names>Romika</given-names></name><xref ref-type="aff" rid="I2"><sup>2</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>Aggarwal</surname><given-names>Neeraj Kumar</given-names></name><xref ref-type="aff" rid="I2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kumar</surname><given-names>Vikas</given-names></name><xref ref-type="aff" rid="I2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kaur</surname><given-names>Manpreeet</given-names></name><xref ref-type="aff" rid="I2"><sup>2</sup></xref></contrib></contrib-group><aff id="I1"><sup>1</sup>Vaidyanath Research, Training and Diagnostic Centre, Kurukshetra 136118, India</aff><aff id="I2"><sup>2</sup>Department of Microbiology, Kurukshetra University, Kurukshetra 136119, India</aff><author-notes><corresp id="cor1">*Romika Dhiman: <email>romikadhiman@gmail.com</email></corresp><fn fn-type="other"><p>Academic Editor: Rosana G. Moreira</p></fn></author-notes><pub-date pub-type="ppub"><year>2014</year></pub-date><pub-date pub-type="epub"><day>16</day><month>10</month><year>2014</year></pub-date><volume>2014</volume><elocation-id>408085</elocation-id><history><date date-type="received"><day>24</day><month>6</month><year>2014</year></date><date date-type="rev-recd"><day>22</day><month>9</month><year>2014</year></date><date date-type="accepted"><day>6</day><month>10</month><year>2014</year></date></history><permissions><copyright-statement>Copyright © 2014 Kamal Rai Aneja et al.</copyright-statement><copyright-year>2014</copyright-year><license license-type="open-access"><license-p>This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><p>Fruit juices are popular drinks as they contain antioxidants, vitamins, and minerals that are essential for human being and play important role in the prevention of heart diseases, cancer, and diabetes. They contain essential nutrients which support the growth of acid tolerant bacteria, yeasts, and moulds. In the present study, we have conducted a microbiological examination of freshly prepared juices (sweet lime, orange, and carrot) by serial dilution agar plate technique. A total of 30 juice samples were examined for their microbiological quality. Twenty-five microbial species including 9 bacterial isolates, 5 yeast isolates, and 11 mould isolates were isolated from juices. Yeasts and moulds were the main cause of spoilage of juices. <italic>Aspergillus flavus</italic> and <italic>Rhodotorula mucilaginosa</italic> were observed in the maximum number of juice samples. Among bacteria <italic>Bacillus cereus</italic> and <italic>Serratia</italic> were dominant. <italic>Escherichia coli</italic> and <italic>Staphylococcus aureus</italic> were detected in few samples. <italic>Candida</italic> sp., <italic>Curvularia</italic>, <italic>Colletotrichum</italic>, and <italic>Acetobacter</italic> were observed only in citrus juice samples. <italic>Alternaria</italic>, <italic>Aspergillus terreus</italic>, <italic>A. niger</italic>, <italic>Cladosporium</italic>, and <italic>Fusarium</italic> were also observed in tested juice samples. Some of the microorganisms detected in these juice samples can cause disease in human beings, so there is need for some guidelines that can improve the quality of fruit juices.</p></abstract></article-meta></front><body><sec id="sec1"><title>1. Introduction</title><p>Unpasteurized fruit juice is defined as the product produced by pressing or squeezing of the fruits [<xref rid="B1" ref-type="bibr">1</xref>]. Consumption of fresh juices increased dramatically due to their freshness, high vitamin content, and low caloric consumption [<xref rid="B2" ref-type="bibr">2</xref>]. Extracted juices from fruits contain most substances which are found in the original ripe and sound fruit from which the juice is made. The high potassium and low sodium characteristic of most juices help in maintaining a healthy blood pressure. Vitamin C is naturally present in juices which are essential for the body to form collagen, cartilage, muscle, and blood vessels. It also helps in the absorption of iron [<xref rid="B3" ref-type="bibr">3</xref>].</p><p>Fruit juices contain a microflora which is normally present on the surface of fruits during harvest and postharvest processing which include transport, storage, and processing [<xref rid="B4" ref-type="bibr">4</xref>]. Many microorganisms such as acid tolerant bacteria and fungi (moulds, yeasts) use them as a substrate for their growth. Yeasts form the main flora of fruits before processing because of acidic pH. The major genera include<italic> Candida</italic>,<italic> Dekkera</italic>,<italic> Hanseniaspora</italic>,<italic> Pichia</italic>,<italic> Saccharomyces</italic>, and<italic> Zygosaccharomyces</italic>.<italic> Penicillium</italic>,<italic> Byssochlamys</italic>,<italic> Aspergillus</italic>,<italic> Paecilomyces</italic>,<italic> Mucor</italic>,<italic> Cladosporium</italic>,<italic> Fusarium</italic>,<italic> Botrytis</italic>,<italic> Talaromyces</italic>, and<italic> Neosartorya</italic> are filamentous fungi most frequently isolated from fresh fruits and juices. Among bacteria, lactic acid bacteria and acetic acid bacteria have been isolated from fruit juices [<xref rid="B5" ref-type="bibr">5</xref>].</p><p>The critical factors affecting the spoilage of juices include juice pH, oxidation reduction potential, water activity, availability of nutrients, presence of antimicrobial compounds, and competing microflora. Among these factors, pH and water activity are the most influential factors affecting the spoilage of juices. The spoilage caused by microorganisms in juices includes cloud loss, development of off-flavours, CO<sub>2</sub> production, and changes in colour, texture, and appearance resulting in degradation of product [<xref rid="B6" ref-type="bibr">6</xref>, <xref rid="B7" ref-type="bibr">7</xref>]. The most commonly reported bacterial genera include<italic> Acetobacter</italic>,<italic> Alicyclobacillus</italic>,<italic> Bacillus</italic>,<italic> Gluconobacter</italic>,<italic> Lactobacillus</italic>,<italic> Leuconostoc</italic>,<italic> Zymomonas</italic>, and<italic> Zymobacter</italic>. Among yeasts<italic> Pichia</italic>,<italic> Candida</italic>,<italic> Saccharomyces</italic>, and<italic> Rhodotorula</italic> are commonly encountered genera responsible for spoilage of juices [<xref rid="B8" ref-type="bibr">8</xref>]. Certain common moulds such as<italic> Penicillium</italic> sp.,<italic> Aspergillus</italic> sp.,<italic> Eurotium</italic>,<italic> Alternaria</italic>,<italic> Cladosporium</italic>,<italic> Paecilomyces</italic>, and<italic> Botrytis</italic> have also been reported in spoilage of fruit juices [<xref rid="B5" ref-type="bibr">5</xref>, <xref rid="B6" ref-type="bibr">6</xref>].</p><p>Fruit juices have pH in the acidic range (<4.5) serving as important barrier for microbial growth. However, food borne pathogens such as<italic> E. coli</italic> and<italic> Salmonella</italic> survive in acidic environment of fruit juices due to acid stress response. Therefore, in the last two decades a number of food borne outbreaks associated with unpasteurized fruit juices have been documented in many countries [<xref rid="B1" ref-type="bibr">1</xref>, <xref rid="B9" ref-type="bibr">9</xref>]. The source of entry of microorganisms into fresh fruit juices from environment exposure and soil. In developing country like India, a large population of all income and age groups consume freshly squeezed fruit and vegetable juice [<xref rid="B10" ref-type="bibr">10</xref>], but the presence of pathogenic microorganisms in street vended fruit juices has also been reported in various parts of India such as Vishakhapatnam [<xref rid="B11" ref-type="bibr">11</xref>], Mumbai [<xref rid="B12" ref-type="bibr">12</xref>], Amravati [<xref rid="B13" ref-type="bibr">13</xref>], and Nagpur [<xref rid="B10" ref-type="bibr">10</xref>]. In view of the demand for fresh fruit juices throughout the year and threat of emerging food borne outbreaks associated with consumption of fruit juices, the aim of present study was to investigate the microbiological examination of freshly prepared juices commonly consumed in Kurukshetra.</p></sec><sec id="sec2"><title>2. Materials and Methods</title><sec id="sec2.1"><title>2.1. Fruit Juice Preparation</title><p>Three juices commonly consumed in Kurukshetra such as orange (<italic>Citrus reticulata</italic> Blanco), sweet orange (<italic>Citrus sinensis</italic>), and carrot (<italic>Daucus carota</italic>) were selected for microbiological study. Sweet orange, carrot, and orange were purchased from the local markets of Kurukshetra from October 2011 to February 2012. Each sample was washed, peeled, and cut into pieces and juice was extracted through sterile hand blender and poured into sterile beaker.</p></sec><sec id="sec2.2"><title>2.2. Measurement of pH</title><p>The pH of juice samples was measured using a pH meter.</p></sec><sec id="sec2.3"><title>2.3. Microbiological Analysis</title><p>The microbiological study of fruit juices was done by serial dilution agar plate technique. Ten mL of juice sample was diluted with 90 mL of 0.1% sterile peptone water (1 g peptone, 1L distilled water) and plated on nutrient agar (pH 5.5) for enumeration of bacteria and PDA supplemented with antibiotic (pH 5.5) for enumeration of fungi in duplicates [<xref rid="B4" ref-type="bibr">4</xref>]. Uninoculated plates of PDA and NA were used as control. Mould and yeast isolates were purified on potato dextrose agar, bacteria on nutrient agar, and further subcultured for microscopic examination and identification.</p></sec><sec id="sec2.4"><title>2.4. Identification of Bacteria</title><p>For bacterial identification, 24-hour-old culture of bacteria was observed under microscope by gram stain method and further various biochemical tests were performed for the identification of bacteria such as catalase test, oxidase test, starch hydrolysis test, sugar fermentation test, IMViC test, and methods described in “Bergey's Manual of Systematic Bacteriology” [<xref rid="B15" ref-type="bibr">14</xref>]. Further identification of bacteria was performed on the basis of methods described in “Compendium of methods for the microbiological examination of foods” [<xref rid="B16" ref-type="bibr">15</xref>, <xref rid="B17" ref-type="bibr">16</xref>].</p></sec><sec id="sec2.5"><title>2.5. Identification of Yeasts</title><p>The methods adopted for identification of yeasts include morphological characteristics, fermentation of sugars, germ tube test and cycloheximide resistance test, and methods described in “Fungi and Food Spoilage” [<xref rid="B18" ref-type="bibr">17</xref>, <xref rid="B19" ref-type="bibr">18</xref>].</p></sec><sec id="sec2.6"><title>2.6. Identification of Moulds</title><p>Moulds were identified on the basis of morphological and cultural characteristics such as colour of the colony, surface, appearance, presence, and absence of cross walls, and asexual and sexual reproductive structures. Further identification of moulds was carried out according to the methods described in “Fungi and Food Spoilage.” Moulds were cultured on Czapek yeast extract agar (pH 6.7), Malt extract agar (pH 5.6), and Glycerol nitrate agar (pH 7.0) at 25°C.</p></sec></sec><sec id="sec3"><title>3. Results and Discussion</title><p>In the present study, 30 samples of freshly prepared juices (10 samples each of orange, sweet orange, and carrot) were examined for microbiological analysis. The pH range of juices is shown in <xref ref-type="table" rid="tab1">Table 1</xref>. Factors which determine the colonization of juices by microorganisms include pH, redox potential, water activity, nutrients, structures, antimicrobial agents, temperature, relative humidity, and atmosphere [<xref rid="B1" ref-type="bibr">1</xref>]. In the present study the frequencies of occurrence of moulds and yeasts were more as compared to bacterial genera which is attributed to low pH values and high sugar content [<xref rid="B20" ref-type="bibr">19</xref>].</p><p>A total of 34 bacterial, 12 yeast, and 25 mould isolates were isolated from juices classified by grouping them into 9 bacterial species, 5 yeast species, and 11 mould species on the basis of phenotypic characteristics. Morphological and biochemical properties of bacteria were explained in Tables <xref ref-type="table" rid="tab2">2</xref> and <xref ref-type="table" rid="tab3">3</xref>. Details of morphology and physiology of yeasts were described in Tables <xref ref-type="table" rid="tab4">4</xref> and <xref ref-type="table" rid="tab5">5</xref>. Colonial and microscopic characteristics of various moulds were summarized in Tables <xref ref-type="table" rid="tab6">6</xref> and <xref ref-type="table" rid="tab7">7</xref>.</p><p>Yeasts and moulds are capable of growth at pH values of 1.5 and at water activity values below 0.89. The minimum pH values allowing the growth of lactic acid bacteria (pH 2.9–3.5), acetic acid bacteria (pH 3.0–4.5), and enteric bacteria (pH 3.6–4.5) are higher than those for growth of yeasts and moulds [<xref rid="B6" ref-type="bibr">6</xref>].</p><p>The frequency of occurrence of bacteria, yeasts, and moulds are summarized in Tables <xref ref-type="table" rid="tab8">8</xref>, <xref ref-type="table" rid="tab9">9</xref>, and <xref ref-type="table" rid="tab10">10</xref>, respectively. The occurrence of bacterial genera ranged from 10% to 56% (<xref ref-type="table" rid="tab8">Table 8</xref>).<italic> Bacillus cereus</italic> and<italic> Serratia</italic> sp. were detected in a greater number of samples.<italic> Bacillus cereus</italic> was also observed in 64.91% of samples of unpasteurized street vended fruit juices [<xref rid="B21" ref-type="bibr">20</xref>].<italic> Leuconostoc</italic> and<italic> Lactobacillus</italic> were also reported as important group of spoilage microorganisms in acidic products [<xref rid="B22" ref-type="bibr">21</xref>]. The presence of lactic acid bacteria more frequently occurs in unpasteurized juices [<xref rid="B23" ref-type="bibr">22</xref>]. These microorganisms produce acetic and formic acids along with ethanol and carbon dioxide which can alter the flavor of juice [<xref rid="B24" ref-type="bibr">23</xref>].<italic> Leuconostoc</italic>,<italic> Lactobacillus</italic>, and<italic> Acetobacter</italic> were detected in tested juice samples (<xref ref-type="table" rid="tab8">Table 8</xref>).</p><p>The presence of<italic> E. coli</italic>,<italic> Salmonella</italic>, and<italic> S. aureus</italic> in fruit juices is primarily concern because these pathogens were implicated in a number of outbreaks associated with fruit juices [<xref rid="B1" ref-type="bibr">1</xref>]. In our study, the presence of<italic> E. coli</italic> and<italic> S. aureus</italic> was detected in a smaller number of samples. The survival of pathogens in acidic environment of juices is attributed to their ability to regulate their internal pH and maintained at neutral pH by combination of passive and active homeostasis mechanisms [<xref rid="B25" ref-type="bibr">24</xref>]. The acid survival mechanisms of enteric bacteria are due to induction of enzymes that are involved in raising the internal pH and activation of enzymes devoted to the protection and repair of proteins and DNA [<xref rid="B26" ref-type="bibr">25</xref>].</p><p>Yeasts genera responsible for spoilage of fruit juices include<italic> Candida</italic>,<italic> Pichia</italic>,<italic> Rhodotorula</italic>,<italic> Torulopsis</italic>,<italic> Saccharomyces</italic>,<italic> Zygosaccharomyces</italic>,<italic> Hansenula</italic>, and<italic> Trichosporon</italic> [<xref rid="B27" ref-type="bibr">26</xref>]. In our study, the dominant yeasts isolated from juices were<italic> Rhodotorula</italic>,<italic> Pichia</italic>, and<italic> Saccharomyces</italic> (<xref ref-type="table" rid="tab9">Table 9</xref>). Rhodotorula was found in maximum number of juice samples tested followed by<italic> Pichia</italic> and<italic> Saccharomyces</italic>.<italic> Candida parapsilosis</italic> and<italic> C. krusei</italic> were only detected in orange and sweet orange juices, not detected in carrot juice. Ghenghesh et al. [<xref rid="B9" ref-type="bibr">9</xref>] also reported the presence of<italic> Candida</italic> sp. in 58% of orange juice samples.<italic> Rhodotorula</italic>,<italic> Pichia</italic>,<italic> Candida</italic>, and<italic> Saccharomyces</italic> have also been reported as spoilage causing organisms in pasteurized fruit juices [<xref rid="B4" ref-type="bibr">4</xref>, <xref rid="B28" ref-type="bibr">27</xref>]. Yeast spoilage in fruit juices is characterized by formation of CO<sub>2</sub> and alcohol. Yeasts may also produce turbidity, flocculation, pellicles, and clumping. Yeasts also produced pectin esterases which degrade pectin causing spoilage; organic acids and acetaldehyde, which contribute to a “fermented flavor,” may also be formed [<xref rid="B5" ref-type="bibr">5</xref>, <xref rid="B6" ref-type="bibr">6</xref>].</p><p>The dominant moulds recorded in fruit juices belong to<italic> Penicillium</italic> sp.,<italic> Cladosporium</italic> sp.,<italic> Aspergillus niger</italic>,<italic> A</italic>.<italic> fumigatus</italic>,<italic> Botrytis</italic> sp., and<italic> Aureobasidium pullulans</italic>. They produce mycelial mats and musty, stale off-flavours in juices [<xref rid="B6" ref-type="bibr">6</xref>].<italic> Rhizopus</italic> and<italic> Mucor</italic> are also associated with spoilage of fresh fruits and vegetables [<xref rid="B29" ref-type="bibr">28</xref>]. In the present study, the most frequently encountered moulds were<italic> Aspergillus flavus</italic>,<italic> A. terreus</italic>, and<italic> Penicillium islandicum</italic> (<xref ref-type="table" rid="tab5">Table 5</xref>).<italic> P. digitatum</italic>,<italic> Colletotrichum</italic>, and<italic> Curvularia</italic> were isolated from orange and sweet orange juices.<italic> Geotrichum</italic> was detected in orange and carrot juice. Spoilage by moulds in fruit juices is characterized by loss of juice cloud [<xref rid="B6" ref-type="bibr">6</xref>]. Among these, some moulds produce mycotoxins which are of great threat to human health. Major mycotoxins associated with fruit juices are byssochlamic acid (<italic>Byssochlamys fulva</italic>,<italic> B. nivea</italic>), patulin (<italic>B. fulva</italic>,<italic> B. nivea</italic>, and<italic> P. expansum</italic>), ochratoxin (<italic>Aspergillus carbonarius</italic>), and citrinin (<italic>Penicillium expansum</italic>,<italic> P. citrinum</italic>) [<xref rid="B30" ref-type="bibr">29</xref>, <xref rid="B31" ref-type="bibr">30</xref>].</p></sec><sec id="sec4"><title>4. Conclusion</title><p>Juices squeezed from fresh fruits and vegetables contain microorganisms which are potentially hazardous to public health. Juices were spoiled with high level of moulds and yeasts which is attributable to low pH of juices. The presence of pathogenic microorganisms in juices is clearly indication of food borne outbreaks. The selling and consumption of juices are never stopped on nutritional grounds as well as livelihood of street vendors. It is alarming situation for suitable agency to take some necessary action, make guidelines to prevent potential food poisoning from juices that contain pathogenic bacteria, and find natural antimicrobials from plants that control spoilage and pathogenic microorganisms in juices.</p></sec></body><back><ack><title>Acknowledgment</title><p>The authors are thankful to Kurukshetra University, Kurukshetra, for the financial assistance.</p></ack><sec sec-type="conflict"><title>Conflict of Interests</title><p>The authors declare that there is no conflict of interests regarding the publication of this paper.</p></sec><ref-list><ref id="B1"><label>1</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Raybaudi-Massilia</surname><given-names>R. 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R.</given-names></name></person-group><article-title>Microbiology of soft drinks and fruit juices</article-title><source><italic>Chemistry and Technology of Soft Drinks and Fruit Juices</italic></source><year>2005</year><publisher-loc>London, UK</publisher-loc><publisher-name>Blackwell Publishing</publisher-name></element-citation></ref><ref id="B31"><label>30</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Delage</surname><given-names>N.</given-names></name><name><surname>Harlingue</surname><given-names>A.</given-names></name><name><surname>Ceccaldi</surname><given-names>B. C.</given-names></name><name><surname>Bompeix</surname><given-names>G.</given-names></name></person-group><article-title>Mycotoxins in fruit juices and wine</article-title><source><italic>Food Control</italic></source><year>2003</year><volume>14</volume><fpage>2252</fpage><lpage>2257</lpage></element-citation></ref></ref-list></back><floats-group><table-wrap id="tab1" orientation="portrait" position="float"><label>Table 1</label><caption><p>pH values of juices.</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" rowspan="1" colspan="1">Juices </th><th align="center" rowspan="1" colspan="1">pH range</th><th align="center" rowspan="1" colspan="1">Mean </th></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1">Orange </td><td align="center" rowspan="1" colspan="1">4.19–4.50</td><td align="center" rowspan="1" colspan="1">4.34</td></tr><tr><td align="left" rowspan="1" colspan="1">Sweet lime </td><td align="center" rowspan="1" colspan="1">4.70–5.47</td><td align="center" rowspan="1" colspan="1">5.08</td></tr><tr><td align="left" rowspan="1" colspan="1">Carrot </td><td align="center" rowspan="1" colspan="1">5.76–6.03</td><td align="center" rowspan="1" colspan="1">5.89</td></tr></tbody></table></table-wrap><table-wrap id="tab2" orientation="portrait" position="float"><label>Table 2</label><caption><p>Morphological characteristics of bacterial isolates of juices.</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" rowspan="1" colspan="1">Bacterial isolates </th><th align="center" rowspan="1" colspan="1">Colour on nutrient agar </th><th align="center" rowspan="1" colspan="1">Configuration </th><th align="center" rowspan="1" colspan="1">Margin </th><th align="center" rowspan="1" colspan="1">Elevation </th><th align="center" rowspan="1" colspan="1">Gram reaction </th><th align="center" rowspan="1" colspan="1">Shape of isolate </th><th align="center" rowspan="1" colspan="1">Endospore staining</th></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1"><italic>Bacillus subtilis </italic></td><td align="center" rowspan="1" colspan="1">White</td><td align="center" rowspan="1" colspan="1">Circular lobate </td><td align="center" rowspan="1" colspan="1">Irregular </td><td align="center" rowspan="1" colspan="1">Flat </td><td align="center" rowspan="1" colspan="1">Positive </td><td align="center" rowspan="1" colspan="1">Rods in chains</td><td align="center" rowspan="1" colspan="1">Central spore </td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>B. cereus </italic></td><td align="center" rowspan="1" colspan="1">Off-white </td><td align="center" rowspan="1" colspan="1">Circular </td><td align="center" rowspan="1" colspan="1">Entire </td><td align="center" rowspan="1" colspan="1">Convex </td><td align="center" rowspan="1" colspan="1">Positive</td><td align="center" rowspan="1" colspan="1">Rods in chains</td><td align="center" rowspan="1" colspan="1">Central spore</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Escherichia coli </italic></td><td align="center" rowspan="1" colspan="1">Mucoid </td><td align="center" rowspan="1" colspan="1">Circular </td><td align="center" rowspan="1" colspan="1">Entire </td><td align="center" rowspan="1" colspan="1">Slightly raised </td><td align="center" rowspan="1" colspan="1">Negative </td><td align="center" rowspan="1" colspan="1">Rods </td><td align="center" rowspan="1" colspan="1">—</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Serratia </italic></td><td align="center" rowspan="1" colspan="1">Mucoid </td><td align="center" rowspan="1" colspan="1">Circular </td><td align="center" rowspan="1" colspan="1">Entire </td><td align="center" rowspan="1" colspan="1">Umbonate </td><td align="center" rowspan="1" colspan="1">Negative</td><td align="center" rowspan="1" colspan="1">Rods </td><td align="center" rowspan="1" colspan="1">—</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Leuconostoc </italic></td><td align="center" rowspan="1" colspan="1">Light yellow</td><td align="center" rowspan="1" colspan="1">Circular </td><td align="center" rowspan="1" colspan="1">Entire </td><td align="center" rowspan="1" colspan="1">Convex </td><td align="center" rowspan="1" colspan="1">Positive</td><td align="center" rowspan="1" colspan="1">Cocci </td><td align="center" rowspan="1" colspan="1">—</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Micrococcus </italic></td><td align="center" rowspan="1" colspan="1">Bright Yellow </td><td align="center" rowspan="1" colspan="1">Circular </td><td align="center" rowspan="1" colspan="1">Entire </td><td align="center" rowspan="1" colspan="1">Convex </td><td align="center" rowspan="1" colspan="1">Positive</td><td align="center" rowspan="1" colspan="1">Cocci shape in tetrad</td><td align="center" rowspan="1" colspan="1">—</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Staphylococcus aureus </italic></td><td align="center" rowspan="1" colspan="1">Golden yellow colour</td><td align="center" rowspan="1" colspan="1">Circular pin head colonies </td><td align="center" rowspan="1" colspan="1">Entire </td><td align="center" rowspan="1" colspan="1">Convex</td><td align="center" rowspan="1" colspan="1">Positive</td><td align="center" rowspan="1" colspan="1">Cocci in grapes like bunches </td><td align="center" rowspan="1" colspan="1">—</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Lactobacillus </italic></td><td align="center" rowspan="1" colspan="1">White </td><td align="center" rowspan="1" colspan="1">Circular </td><td align="center" rowspan="1" colspan="1">Entire </td><td align="center" rowspan="1" colspan="1">Raised </td><td align="center" rowspan="1" colspan="1">Positive</td><td align="center" rowspan="1" colspan="1">Rods </td><td align="center" rowspan="1" colspan="1">—</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Acetobacter </italic></td><td align="center" rowspan="1" colspan="1">Pale </td><td align="center" rowspan="1" colspan="1">Circular </td><td align="center" rowspan="1" colspan="1">Entire </td><td align="center" rowspan="1" colspan="1">Flat </td><td align="center" rowspan="1" colspan="1">Negative</td><td align="center" rowspan="1" colspan="1">Rods </td><td align="center" rowspan="1" colspan="1">—</td></tr></tbody></table><table-wrap-foot><fn><p>—: absent.</p></fn></table-wrap-foot></table-wrap><table-wrap id="tab3" orientation="portrait" position="float"><label>Table 3</label><caption><p>Biochemical characteristics of bacterial isolates of juices.</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" rowspan="2" colspan="1">Bacterial isolates </th><th align="center" rowspan="2" colspan="1">Catalase </th><th align="center" rowspan="2" colspan="1">Oxidase </th><th align="center" rowspan="2" colspan="1">Starch hydrolysis</th><th align="center" colspan="4" rowspan="1">IMViC test</th><th align="center" colspan="4" rowspan="1">Sugar fermentation</th></tr><tr><th align="center" rowspan="1" colspan="1">Indole </th><th align="center" rowspan="1" colspan="1">Methyl red </th><th align="center" rowspan="1" colspan="1">Voges-Proskauer</th><th align="center" rowspan="1" colspan="1">Citrate </th><th align="center" rowspan="1" colspan="1">Glucose </th><th align="center" rowspan="1" colspan="1">Lactose </th><th align="center" rowspan="1" colspan="1">Mannitol </th><th align="center" rowspan="1" colspan="1">Sucrose </th></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1"><italic>Bacillus subtilis </italic></td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">A</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">A</td><td align="center" rowspan="1" colspan="1">A</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>B. cereus </italic></td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">A</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">A</td><td align="center" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Escherichia coli </italic></td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">A + G</td><td align="center" rowspan="1" colspan="1">A + G</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Serratia </italic></td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">A</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Leuconostoc </italic></td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">A</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">A</td><td align="center" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Micrococcus </italic></td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">A</td><td align="center" rowspan="1" colspan="1">A</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Staphylococcus aureus </italic></td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">A</td><td align="center" rowspan="1" colspan="1">A</td><td align="center" rowspan="1" colspan="1">A</td><td align="center" rowspan="1" colspan="1">A</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Lactobacillus </italic></td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">A + G</td><td align="center" rowspan="1" colspan="1">A</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Acetobacter </italic></td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">A</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td></tr></tbody></table><table-wrap-foot><fn><p>+: positive; −: negative; A: acid; A + G: acid + gas.</p></fn></table-wrap-foot></table-wrap><table-wrap id="tab4" orientation="portrait" position="float"><label>Table 4</label><caption><p>Morphological details of yeast isolates of juices.</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" rowspan="1" colspan="1">Yeast isolates </th><th align="center" rowspan="1" colspan="1">Colour on PDA</th><th align="center" rowspan="1" colspan="1">Configuration </th><th align="center" rowspan="1" colspan="1">Margin </th><th align="center" rowspan="1" colspan="1">Microscopic features</th></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1"><italic>Pichia </italic></td><td align="center" rowspan="1" colspan="1">Off-white </td><td align="center" rowspan="1" colspan="1">Hemispherical </td><td align="center" rowspan="1" colspan="1">Irregular </td><td align="center" rowspan="1" colspan="1">Ellipsoidal to cylindrical; reproducing by irregular budding </td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Saccharomyces </italic></td><td align="center" rowspan="1" colspan="1">Off-white</td><td align="center" rowspan="1" colspan="1">Circular </td><td align="center" rowspan="1" colspan="1">Irregular </td><td align="center" rowspan="1" colspan="1">Spherical to subspheroidal; reproducing by irregular budding </td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Candida krusei </italic></td><td align="center" rowspan="1" colspan="1">White </td><td align="center" rowspan="1" colspan="1">Circular </td><td align="center" rowspan="1" colspan="1">Irregular </td><td align="center" rowspan="1" colspan="1">Ellipsoidal to long cylindrical; reproducing by irregular budding </td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Rhodotorula </italic></td><td align="center" rowspan="1" colspan="1">Pink </td><td align="center" rowspan="1" colspan="1">Circular or spreading </td><td align="center" rowspan="1" colspan="1">Regular </td><td align="center" rowspan="1" colspan="1">Ellipsoidal shape; reproducing by irregular budding </td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Candida parapsilosis </italic></td><td align="center" rowspan="1" colspan="1">White to cream </td><td align="center" rowspan="1" colspan="1">Circular </td><td align="center" rowspan="1" colspan="1">Regular </td><td align="center" rowspan="1" colspan="1">Globose to ovoid budding</td></tr></tbody></table></table-wrap><table-wrap id="tab5" orientation="portrait" position="float"><label>Table 5</label><caption><p>Physiological tests for yeasts isolates of juices.</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" rowspan="2" colspan="1">Yeast isolate </th><th align="center" rowspan="2" colspan="1">Germ tube test</th><th align="center" rowspan="2" colspan="1">Cycloheximide resistance </th><th align="center" colspan="4" rowspan="1">Sugar fermentation∗</th></tr><tr><th align="center" rowspan="1" colspan="1">Glucose </th><th align="center" rowspan="1" colspan="1">Sucrose </th><th align="center" rowspan="1" colspan="1">Lactose </th><th align="center" rowspan="1" colspan="1">Maltose </th></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1"><italic>Pichia </italic></td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Saccharomyces </italic></td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Candida krusei </italic></td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Rhodotorula </italic></td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Candida parapsilosis </italic></td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">+</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td><td align="center" rowspan="1" colspan="1">−</td></tr></tbody></table><table-wrap-foot><fn><p>+: positive; −: negative; ∗fermentation means production of gas independent of pH changes.</p></fn></table-wrap-foot></table-wrap><table-wrap id="tab6" orientation="portrait" position="float"><label>Table 6</label><caption><p>Morphological details of mould isolates of juices.</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" rowspan="1" colspan="1">Mould isolate </th><th align="center" rowspan="1" colspan="1">Colony colour on PDA on front side </th><th align="center" rowspan="1" colspan="1">Colony colour on PDA on reverse side</th><th align="left" rowspan="1" colspan="1">Microscopic features </th></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1"><italic>Aspergillus flavus </italic></td><td align="center" rowspan="1" colspan="1">Yellow green </td><td align="center" rowspan="1" colspan="1">Colourless </td><td align="left" rowspan="1" colspan="1">Conidiophores arise separately from foot cell, phialides uniseriate and sometimes biseriate; conidia globose to subglobose</td></tr><tr><td align="center" colspan="4" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>A. terreus </italic></td><td align="center" rowspan="1" colspan="1">Brown</td><td align="center" rowspan="1" colspan="1">Colorless</td><td align="left" rowspan="1" colspan="1">Conidiophore borne from surface hyphae, stripes long, and smooth walled; vesicles with densely packed, short, narrow metulae and phialides; conidia unicellular, spherical, and very small </td></tr><tr><td align="center" colspan="4" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>A. niger </italic></td><td align="center" rowspan="1" colspan="1">Black </td><td align="center" rowspan="1" colspan="1">Creamy </td><td align="left" rowspan="1" colspan="1">Hyphae septate and hyaline, smooth walled conidiophores arising from foot cell; vesicles globose, whole vesicle fertile bearing two series of sterigmata; catenate conidia arranged in basipetal manner, unicellular, and globose </td></tr><tr><td align="center" colspan="4" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Penicillium islandicum </italic></td><td align="center" rowspan="1" colspan="1">Ivy green </td><td align="center" rowspan="1" colspan="1">Creamy</td><td align="left" rowspan="1" colspan="1">Short conidiophores bearing a compact verticil of metulae, phialides closely packed in clusters bearing catenate conidia arranged in basipetal manner, conidia elliptical, smooth, and hyaline </td></tr><tr><td align="center" colspan="4" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>P. digitatum </italic></td><td align="center" rowspan="1" colspan="1">Green </td><td align="center" rowspan="1" colspan="1">Colourless </td><td align="left" rowspan="1" colspan="1">Conidiophores borne from surface and aerial hyphae with thin smooth walls; bearing terminal penicilli; terverticillate but frequently biverticillate or irregular</td></tr><tr><td align="center" colspan="4" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Alternaria </italic></td><td align="center" rowspan="1" colspan="1">Black </td><td align="center" rowspan="1" colspan="1">Colourless </td><td align="left" rowspan="1" colspan="1">Small to large sized conidia with beak; arising in chains in acropetal manner with both transverse and longitudinal septa </td></tr><tr><td align="center" colspan="4" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Cladosporium </italic></td><td align="center" rowspan="1" colspan="1">Black</td><td align="center" rowspan="1" colspan="1">Colourless</td><td align="left" rowspan="1" colspan="1">Conidiophore tall, dark upright, and branched variously near the apex, conidia 1-2-celled ovoid to cylindrical shape</td></tr><tr><td align="center" colspan="4" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Colletotrichum </italic></td><td align="center" rowspan="1" colspan="1">Cottony white to pale gray mycelium </td><td align="center" rowspan="1" colspan="1">Colourless </td><td align="left" rowspan="1" colspan="1">Acervuli disc shaped, typically with dark spines or setae at the edge of conidiophores; conidiophores simple, elongate conidia single celled, hyaline or brightly coloured, cylindrical or pointed, straight or curved </td></tr><tr><td align="center" colspan="4" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Curvularia </italic></td><td align="center" rowspan="1" colspan="1">Green to black </td><td align="center" rowspan="1" colspan="1">Black</td><td align="left" rowspan="1" colspan="1">Simple conidiophores bearing spores apically; Conidia dark, end cells, 3–5-celled; more or less fusiform, typically bent </td></tr><tr><td align="center" colspan="4" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Fusarium </italic></td><td align="center" rowspan="1" colspan="1">Wooly white </td><td align="center" rowspan="1" colspan="1">Colourless </td><td align="left" rowspan="1" colspan="1">Conidiophores slender and simple, short or branched irregularly or bearing a whorl of phialides; conidia hyaline, variable, principally of two kinds, macroconidia several celled slightly curved or bent at the point ends, microconidia 1-celled, ovoid or oblong, borne singly or in chains </td></tr><tr><td align="center" colspan="4" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Geotrichum </italic></td><td align="center" rowspan="1" colspan="1">White </td><td align="center" rowspan="1" colspan="1">Colourless</td><td align="left" rowspan="1" colspan="1">Conidia borne solely by the breakup of hyphae to form arthroconidia </td></tr></tbody></table></table-wrap><table-wrap id="tab7" orientation="portrait" position="float"><label>Table 7</label><caption><p>Colonial characteristics of different moulds' isolates of juices on CYA, MEA, and G25N media.</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" rowspan="2" colspan="1">Mould isolate</th><th align="center" colspan="2" rowspan="1">Colony colour on CYA</th><th align="center" colspan="2" rowspan="1">Colony colour on MEA</th><th align="center" colspan="2" rowspan="1">Colony colour on G25N</th></tr><tr><th align="center" rowspan="1" colspan="1">Front side </th><th align="center" rowspan="1" colspan="1">Reverse side </th><th align="center" rowspan="1" colspan="1">Front side </th><th align="center" rowspan="1" colspan="1">Reverse side</th><th align="center" rowspan="1" colspan="1">Front side </th><th align="center" rowspan="1" colspan="1">Reverse side</th></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1"><italic>Aspergillus flavus </italic></td><td align="center" rowspan="1" colspan="1">Yellow green </td><td align="center" rowspan="1" colspan="1">Colourless </td><td align="center" rowspan="1" colspan="1">Yellow green </td><td align="center" rowspan="1" colspan="1">Colourless </td><td align="center" rowspan="1" colspan="1">Yellow green </td><td align="center" rowspan="1" colspan="1">Colourless </td></tr><tr><td align="center" colspan="7" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>A. terreus </italic></td><td align="center" rowspan="1" colspan="1">Brown </td><td align="center" rowspan="1" colspan="1">Dull brown </td><td align="center" rowspan="1" colspan="1">Brown </td><td align="center" rowspan="1" colspan="1">Dull brown </td><td align="center" rowspan="1" colspan="1">Brown </td><td align="center" rowspan="1" colspan="1">Dull brown </td></tr><tr><td align="center" colspan="7" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>A. niger </italic></td><td align="center" rowspan="1" colspan="1">Black </td><td align="center" rowspan="1" colspan="1">Pale to bright yellow </td><td align="center" rowspan="1" colspan="1">Black </td><td align="center" rowspan="1" colspan="1">Pale to bright yellow </td><td align="center" rowspan="1" colspan="1">Black </td><td align="center" rowspan="1" colspan="1">Pale to bright yellow </td></tr><tr><td align="center" colspan="7" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Penicillium islandicum </italic></td><td align="center" rowspan="1" colspan="1">Greyish green</td><td align="center" rowspan="1" colspan="1">Orange to rust brown central area</td><td align="center" rowspan="1" colspan="1">Greyish green</td><td align="center" rowspan="1" colspan="1">Orange to rust brown central area</td><td align="center" rowspan="1" colspan="1">Greyish green</td><td align="center" rowspan="1" colspan="1">Orange to rust brown central area</td></tr><tr><td align="center" colspan="7" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>P. digitatum </italic></td><td align="center" rowspan="1" colspan="1">Greyish green to olive </td><td align="center" rowspan="1" colspan="1">Pale or brown </td><td align="center" rowspan="1" colspan="1">Dull yellow green </td><td align="center" rowspan="1" colspan="1">Pale or brown</td><td align="center" rowspan="1" colspan="1">Green olive </td><td align="center" rowspan="1" colspan="1">Pale </td></tr><tr><td align="center" colspan="7" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Alternaria </italic></td><td align="center" rowspan="1" colspan="1">Grey to black</td><td align="center" rowspan="1" colspan="1">Black </td><td align="center" rowspan="1" colspan="1">Grey to black</td><td align="center" rowspan="1" colspan="1">Black </td><td align="center" rowspan="1" colspan="1">Grey to black</td><td align="center" rowspan="1" colspan="1">Black </td></tr><tr><td align="center" colspan="7" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Cladosporium </italic></td><td align="center" rowspan="1" colspan="1">Olive to dark olive </td><td align="center" rowspan="1" colspan="1">Grey </td><td align="center" rowspan="1" colspan="1">Olive </td><td align="center" rowspan="1" colspan="1">Grey </td><td align="center" rowspan="1" colspan="1">Olive </td><td align="center" rowspan="1" colspan="1">Black </td></tr><tr><td align="center" colspan="7" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Colletotrichum </italic></td><td align="center" rowspan="1" colspan="1">Grey </td><td align="center" rowspan="1" colspan="1">Pale grey </td><td align="center" rowspan="1" colspan="1">Grey </td><td align="center" rowspan="1" colspan="1">Pale grey </td><td align="center" rowspan="1" colspan="1">Black </td><td align="center" rowspan="1" colspan="1">Grey </td></tr><tr><td align="center" colspan="7" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Curvularia </italic></td><td align="center" rowspan="1" colspan="1">Off-white to grey </td><td align="center" rowspan="1" colspan="1">Grey </td><td align="center" rowspan="1" colspan="1">Off-white to grey </td><td align="center" rowspan="1" colspan="1">Grey </td><td align="center" rowspan="1" colspan="1">Grey to black</td><td align="center" rowspan="1" colspan="1">Grey </td></tr><tr><td align="center" colspan="7" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Fusarium </italic></td><td align="center" rowspan="1" colspan="1">White to grayish rose </td><td align="center" rowspan="1" colspan="1">Pale </td><td align="center" rowspan="1" colspan="1">White </td><td align="center" rowspan="1" colspan="1">Pale </td><td align="center" rowspan="1" colspan="1">White </td><td align="center" rowspan="1" colspan="1">Pale </td></tr><tr><td align="center" colspan="7" rowspan="1"><hr></hr></td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Geotrichum </italic></td><td align="center" rowspan="1" colspan="1">White </td><td align="center" rowspan="1" colspan="1">Pale </td><td align="center" rowspan="1" colspan="1">White </td><td align="center" rowspan="1" colspan="1">Pale </td><td align="center" colspan="2" rowspan="1">No growth</td></tr></tbody></table></table-wrap><table-wrap id="tab8" orientation="portrait" position="float"><label>Table 8</label><caption><p>Percentage abundance of bacterial isolates in juice samples.</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" rowspan="1" colspan="1">Bacterial isolates</th><th align="center" rowspan="1" colspan="1">Orange juice <break></break>(<italic>n</italic> = 10)</th><th align="center" rowspan="1" colspan="1">Sweet orange juice<break></break>(<italic>n</italic> = 10)</th><th align="center" rowspan="1" colspan="1">Carrot juice<break></break>(<italic>n</italic> = 10)</th><th align="center" rowspan="1" colspan="1">Total number of samples</th><th align="center" rowspan="1" colspan="1">Percentage frequency </th></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1"><italic>Bacillus subtilis </italic></td><td align="center" rowspan="1" colspan="1">5</td><td align="center" rowspan="1" colspan="1">3</td><td align="center" rowspan="1" colspan="1">4</td><td align="center" rowspan="1" colspan="1">12</td><td align="center" rowspan="1" colspan="1">40%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>B. cereus </italic></td><td align="center" rowspan="1" colspan="1">8</td><td align="center" rowspan="1" colspan="1">4</td><td align="center" rowspan="1" colspan="1">5</td><td align="center" rowspan="1" colspan="1">17</td><td align="center" rowspan="1" colspan="1">56.7%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Escherichia coli </italic></td><td align="center" rowspan="1" colspan="1">2</td><td align="center" rowspan="1" colspan="1">3</td><td align="center" rowspan="1" colspan="1">1</td><td align="center" rowspan="1" colspan="1">6</td><td align="center" rowspan="1" colspan="1">20.0%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Serratia </italic></td><td align="center" rowspan="1" colspan="1">5</td><td align="center" rowspan="1" colspan="1">5</td><td align="center" rowspan="1" colspan="1">4</td><td align="center" rowspan="1" colspan="1">14</td><td align="center" rowspan="1" colspan="1">46.7%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Leuconostoc </italic></td><td align="center" rowspan="1" colspan="1">4</td><td align="center" rowspan="1" colspan="1">5</td><td align="center" rowspan="1" colspan="1">—</td><td align="center" rowspan="1" colspan="1">9</td><td align="center" rowspan="1" colspan="1">30.0%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Micrococcus </italic></td><td align="center" rowspan="1" colspan="1">—</td><td align="center" rowspan="1" colspan="1">3</td><td align="center" rowspan="1" colspan="1">—</td><td align="center" rowspan="1" colspan="1">3</td><td align="center" rowspan="1" colspan="1">10.0%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Staphylococcus aureus </italic></td><td align="center" rowspan="1" colspan="1">2</td><td align="center" rowspan="1" colspan="1">1</td><td align="center" rowspan="1" colspan="1">2</td><td align="center" rowspan="1" colspan="1">5</td><td align="center" rowspan="1" colspan="1">16.7%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Lactobacillus </italic></td><td align="center" rowspan="1" colspan="1">2</td><td align="center" rowspan="1" colspan="1">3</td><td align="center" rowspan="1" colspan="1">2</td><td align="center" rowspan="1" colspan="1">7</td><td align="center" rowspan="1" colspan="1">23.3%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Acetobacter </italic></td><td align="center" rowspan="1" colspan="1">3</td><td align="center" rowspan="1" colspan="1">5</td><td align="center" rowspan="1" colspan="1">—</td><td align="center" rowspan="1" colspan="1">8</td><td align="center" rowspan="1" colspan="1">26.7%</td></tr></tbody></table><table-wrap-foot><fn><p>—: absent.</p></fn></table-wrap-foot></table-wrap><table-wrap id="tab9" orientation="portrait" position="float"><label>Table 9</label><caption><p>Percentage abundance of yeast isolates in juice samples.</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" rowspan="1" colspan="1">Yeast isolates </th><th align="center" rowspan="1" colspan="1">Orange juice <break></break>(<italic>n</italic> = 10)</th><th align="center" rowspan="1" colspan="1">Sweet orange juice <break></break>(<italic>n</italic> = 10)</th><th align="center" rowspan="1" colspan="1">Carrot juice <break></break>(<italic>n</italic> = 10)</th><th align="center" rowspan="1" colspan="1">Total number of samples </th><th align="center" rowspan="1" colspan="1">Percentage frequency </th></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1"><italic>Pichia </italic></td><td align="center" rowspan="1" colspan="1">8</td><td align="center" rowspan="1" colspan="1">7</td><td align="center" rowspan="1" colspan="1">4</td><td align="center" rowspan="1" colspan="1">19</td><td align="center" rowspan="1" colspan="1">63.3%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Saccharomyces </italic></td><td align="center" rowspan="1" colspan="1">6</td><td align="center" rowspan="1" colspan="1">8</td><td align="center" rowspan="1" colspan="1">4</td><td align="center" rowspan="1" colspan="1">17</td><td align="center" rowspan="1" colspan="1">56.67%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Candida krusei </italic></td><td align="center" rowspan="1" colspan="1">3</td><td align="center" rowspan="1" colspan="1">8</td><td align="center" rowspan="1" colspan="1">—</td><td align="center" rowspan="1" colspan="1">11</td><td align="center" rowspan="1" colspan="1">36.7%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Rhodotorula </italic></td><td align="center" rowspan="1" colspan="1">9</td><td align="center" rowspan="1" colspan="1">6</td><td align="center" rowspan="1" colspan="1">9</td><td align="center" rowspan="1" colspan="1">24</td><td align="center" rowspan="1" colspan="1">80%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Candida parapsilosis </italic></td><td align="center" rowspan="1" colspan="1">8</td><td align="center" rowspan="1" colspan="1">—</td><td align="center" rowspan="1" colspan="1">—</td><td align="center" rowspan="1" colspan="1">8</td><td align="center" rowspan="1" colspan="1">26.7%</td></tr></tbody></table><table-wrap-foot><fn><p>—: absent.</p></fn></table-wrap-foot></table-wrap><table-wrap id="tab10" orientation="portrait" position="float"><label>Table 10</label><caption><p>Percentage abundance of mould isolates in juice samples.</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" rowspan="1" colspan="1">Mould isolates </th><th align="center" rowspan="1" colspan="1">Orange juice <break></break>(<italic>n</italic> = 10)</th><th align="center" rowspan="1" colspan="1">Sweet lime juice <break></break>(<italic>n</italic> = 10)</th><th align="center" rowspan="1" colspan="1">Carrot juice<break></break>(<italic>n</italic> = 10)</th><th align="center" rowspan="1" colspan="1">Total number of samples </th><th align="center" rowspan="1" colspan="1">Percentage frequency = occurrence in observed samples/total no of samples </th></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1"><italic>Aspergillus flavus </italic></td><td align="center" rowspan="1" colspan="1">9</td><td align="center" rowspan="1" colspan="1">9</td><td align="center" rowspan="1" colspan="1">7</td><td align="center" rowspan="1" colspan="1">25</td><td align="center" rowspan="1" colspan="1">83.3%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>A. terreus </italic></td><td align="center" rowspan="1" colspan="1">5</td><td align="center" rowspan="1" colspan="1">7</td><td align="center" rowspan="1" colspan="1">4</td><td align="center" rowspan="1" colspan="1">16</td><td align="center" rowspan="1" colspan="1">53.3%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>A. niger </italic></td><td align="center" rowspan="1" colspan="1">3</td><td align="center" rowspan="1" colspan="1">2</td><td align="center" rowspan="1" colspan="1">1</td><td align="center" rowspan="1" colspan="1">6</td><td align="center" rowspan="1" colspan="1">20%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Penicillium islandicum </italic></td><td align="center" rowspan="1" colspan="1">9</td><td align="center" rowspan="1" colspan="1">7</td><td align="center" rowspan="1" colspan="1">7</td><td align="center" rowspan="1" colspan="1">23</td><td align="center" rowspan="1" colspan="1">76.7%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>P. digitatum</italic></td><td align="center" rowspan="1" colspan="1">7</td><td align="center" rowspan="1" colspan="1">5</td><td align="center" rowspan="1" colspan="1">—</td><td align="center" rowspan="1" colspan="1">12</td><td align="center" rowspan="1" colspan="1">40%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Alternaria </italic></td><td align="center" rowspan="1" colspan="1">6</td><td align="center" rowspan="1" colspan="1">5</td><td align="center" rowspan="1" colspan="1">3</td><td align="center" rowspan="1" colspan="1">14</td><td align="center" rowspan="1" colspan="1">46.7%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Cladosporium </italic></td><td align="center" rowspan="1" colspan="1">3</td><td align="center" rowspan="1" colspan="1">4</td><td align="center" rowspan="1" colspan="1">2</td><td align="center" rowspan="1" colspan="1">9</td><td align="center" rowspan="1" colspan="1">30%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Colletotrichum </italic></td><td align="center" rowspan="1" colspan="1">5</td><td align="center" rowspan="1" colspan="1">3</td><td align="center" rowspan="1" colspan="1">—</td><td align="center" rowspan="1" colspan="1">8</td><td align="center" rowspan="1" colspan="1">26.7%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Curvularia </italic></td><td align="center" rowspan="1" colspan="1">3</td><td align="center" rowspan="1" colspan="1">2</td><td align="center" rowspan="1" colspan="1">—</td><td align="center" rowspan="1" colspan="1">5</td><td align="center" rowspan="1" colspan="1">16.7%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Fusarium </italic></td><td align="center" rowspan="1" colspan="1">4</td><td align="center" rowspan="1" colspan="1">6</td><td align="center" rowspan="1" colspan="1">3</td><td align="center" rowspan="1" colspan="1">13</td><td align="center" rowspan="1" colspan="1">43.3%</td></tr><tr><td align="left" rowspan="1" colspan="1"><italic>Geotrichum </italic></td><td align="center" rowspan="1" colspan="1">4</td><td align="center" rowspan="1" colspan="1">—</td><td align="center" rowspan="1" colspan="1">3</td><td align="center" rowspan="1" colspan="1">7</td><td align="center" rowspan="1" colspan="1">23.3%</td></tr></tbody></table><table-wrap-foot><fn><p>—: absent.</p></fn></table-wrap-foot></table-wrap></floats-group></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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