A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh1[OPEN]
Identifieur interne : 000021 ( Pmc/Corpus ); précédent : 000020; suivant : 000022A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh1[OPEN]
Auteurs : Yunliu Zeng ; Jiabin Du ; Lun Wang ; Zhiyong Pan ; Qiang Xu ; Shunyuan Xiao ; Xiuxin DengSource :
- Plant Physiology [ 0032-0889 ] ; 2015.
Abstract
Two major chromoplasts are region-specifically formed in citrus and converted from amyloplast precursors.
Url:
DOI: 10.1104/pp.15.00645
PubMed: 26056088
PubMed Central: 4528763
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh<xref ref-type="fn" rid="fn1"><sup>1</sup></xref><xref ref-type="fn" rid="fn2"><sup>[OPEN]</sup></xref></title><author><name sortKey="Zeng, Yunliu" sort="Zeng, Yunliu" uniqKey="Zeng Y" first="Yunliu" last="Zeng">Yunliu Zeng</name></author><author><name sortKey="Du, Jiabin" sort="Du, Jiabin" uniqKey="Du J" first="Jiabin" last="Du">Jiabin Du</name></author><author><name sortKey="Wang, Lun" sort="Wang, Lun" uniqKey="Wang L" first="Lun" last="Wang">Lun Wang</name></author><author><name sortKey="Pan, Zhiyong" sort="Pan, Zhiyong" uniqKey="Pan Z" first="Zhiyong" last="Pan">Zhiyong Pan</name></author><author><name sortKey="Xu, Qiang" sort="Xu, Qiang" uniqKey="Xu Q" first="Qiang" last="Xu">Qiang Xu</name></author><author><name sortKey="Xiao, Shunyuan" sort="Xiao, Shunyuan" uniqKey="Xiao S" first="Shunyuan" last="Xiao">Shunyuan Xiao</name></author><author><name sortKey="Deng, Xiuxin" sort="Deng, Xiuxin" uniqKey="Deng X" first="Xiuxin" last="Deng">Xiuxin Deng</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26056088</idno><idno type="pmc">4528763</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4528763</idno><idno type="RBID">PMC:4528763</idno><idno type="doi">10.1104/pp.15.00645</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000021</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh<xref ref-type="fn" rid="fn1"><sup>1</sup></xref><xref ref-type="fn" rid="fn2"><sup>[OPEN]</sup></xref></title><author><name sortKey="Zeng, Yunliu" sort="Zeng, Yunliu" uniqKey="Zeng Y" first="Yunliu" last="Zeng">Yunliu Zeng</name></author><author><name sortKey="Du, Jiabin" sort="Du, Jiabin" uniqKey="Du J" first="Jiabin" last="Du">Jiabin Du</name></author><author><name sortKey="Wang, Lun" sort="Wang, Lun" uniqKey="Wang L" first="Lun" last="Wang">Lun Wang</name></author><author><name sortKey="Pan, Zhiyong" sort="Pan, Zhiyong" uniqKey="Pan Z" first="Zhiyong" last="Pan">Zhiyong Pan</name></author><author><name sortKey="Xu, Qiang" sort="Xu, Qiang" uniqKey="Xu Q" first="Qiang" last="Xu">Qiang Xu</name></author><author><name sortKey="Xiao, Shunyuan" sort="Xiao, Shunyuan" uniqKey="Xiao S" first="Shunyuan" last="Xiao">Shunyuan Xiao</name></author><author><name sortKey="Deng, Xiuxin" sort="Deng, Xiuxin" uniqKey="Deng X" first="Xiuxin" last="Deng">Xiuxin Deng</name></author></analytic><series><title level="j">Plant Physiology</title><idno type="ISSN">0032-0889</idno><idno type="eISSN">1532-2548</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Two major chromoplasts are region-specifically formed in citrus and converted from amyloplast precursors.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id><journal-id journal-id-type="iso-abbrev">Plant Physiol</journal-id><journal-id journal-id-type="hwp">plantphysiol</journal-id><journal-id journal-id-type="publisher-id">aspb</journal-id><journal-title-group><journal-title>Plant Physiology</journal-title></journal-title-group><issn pub-type="ppub">0032-0889</issn><issn pub-type="epub">1532-2548</issn><publisher><publisher-name>American Society of Plant Biologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26056088</article-id><article-id pub-id-type="pmc">4528763</article-id><article-id pub-id-type="publisher-id">PP201500645</article-id><article-id pub-id-type="doi">10.1104/pp.15.00645</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject><subj-group subj-group-type="heading"><subject>Genes, Development, and Evolution</subject></subj-group></subj-group></article-categories><title-group><article-title>A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh<xref ref-type="fn" rid="fn1"><sup>1</sup></xref><xref ref-type="fn" rid="fn2"><sup>[OPEN]</sup></xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Zeng</surname><given-names>Yunliu</given-names></name></contrib><contrib contrib-type="author"><name><surname>Du</surname><given-names>Jiabin</given-names></name></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Lun</given-names></name></contrib><contrib contrib-type="author"><name><surname>Pan</surname><given-names>Zhiyong</given-names></name></contrib><contrib contrib-type="author"><name><surname>Xu</surname><given-names>Qiang</given-names></name></contrib><contrib contrib-type="author"><name><surname>Xiao</surname><given-names>Shunyuan</given-names></name></contrib><contrib contrib-type="author"><name><surname>Deng</surname><given-names>Xiuxin</given-names></name><xref ref-type="corresp" rid="cor1">*</xref></contrib><aff id="aff1">Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China (Y.Z., J.D., L.W., Z.P., Q.X., S.X., X.D.); and</aff><aff id="aff2">Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850 (S.X.)</aff></contrib-group><author-notes><fn><p><ext-link ext-link-type="uri" xlink:href="http://www.plantphysiol.org/cgi/doi/10.1104/pp.15.00645">www.plantphysiol.org/cgi/doi/10.1104/pp.15.00645</ext-link></p></fn><corresp id="cor1"><label>*</label>Address correspondence to <email>xxdeng@mail.hzau.edu.cn</email>.</corresp><fn id="afn1"><p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (<ext-link ext-link-type="uri" xlink:href="http://www.plantphysiol.org">www.plantphysiol.org</ext-link>) is: Xiuxin Deng (<email>xxdeng@mail.hzau.edu.cn</email>).</p></fn><fn id="afn2"><p>X.D. and Z.P. conceived the original screening and research plans; Y.Z. designed the experiments, performed most of the experiments, analyzed the data, conceived the project, and wrote the article with contributions of all the authors; J.D. and L.W. provided technical assistance to Y.Z.; X.D., Q.X., and S.X. supervised and complemented the writing.</p></fn></author-notes><pmc-comment>Fake ppub date generated by PMC from publisher
pub-date/@pub-type='epub-ppub' </pmc-comment>
<pub-date pub-type="ppub"><month>8</month><year>2015</year></pub-date><pub-date pub-type="epub"><day>08</day><month>6</month><year>2015</year></pub-date><pub-date pub-type="pmc-release"><day>08</day><month>6</month><year>2015</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>168</volume><issue>4</issue><fpage>1648</fpage><lpage>1665</lpage><history><date date-type="received"><day>30</day><month>4</month><year>2015</year></date><date date-type="accepted"><day>05</day><month>6</month><year>2015</year></date></history><permissions><copyright-statement>© 2015 American Society of Plant Biologists. All Rights Reserved.</copyright-statement><copyright-year>2015</copyright-year></permissions><self-uri xlink:role="icon" xlink:href="PP_PP201500645_ic1.gif"></self-uri><abstract abstract-type="precis"><p>Two major chromoplasts are region-specifically formed in citrus and converted from amyloplast precursors.</p></abstract><abstract><p>Globular and crystalloid chromoplasts were observed to be region specifically formed in sweet orange (<italic>Citrus sinensis</italic>) flesh and converted from amyloplasts during fruit maturation, which was associated with the composition of specific carotenoids and the expression of carotenogenic genes. Subsequent isobaric tag for relative and absolute quantitation (<xref ref-type="def" rid="def1">iTRAQ</xref>)-based quantitative proteomic analyses of purified plastids from the flesh during chromoplast differentiation and senescence identified 1,386 putative plastid-localized proteins, 1,016 of which were quantified by spectral counting. The <xref ref-type="def" rid="def1">iTRAQ</xref> values reflecting the expression abundance of three identified proteins were validated by immunoblotting. Based on <xref ref-type="def" rid="def1">iTRAQ</xref> data, chromoplastogenesis appeared to be associated with three major protein expression patterns: (1) marked decrease in abundance of the proteins participating in the translation machinery through ribosome assembly; (2) increase in abundance of the proteins involved in terpenoid biosynthesis (including carotenoids), stress responses (redox, ascorbate, and glutathione), and development; and (3) maintenance of the proteins for signaling and DNA and RNA. Interestingly, a strong increase in abundance of several plastoglobule-localized proteins coincided with the formation of plastoglobules in the chromoplast. The proteomic data also showed that stable functioning of protein import, suppression of ribosome assembly, and accumulation of chromoplast proteases are correlated with the amyloplast-to-chromoplast transition; thus, these processes may play a collective role in chromoplast biogenesis and differentiation. By contrast, the chromoplast senescence process was inferred to be associated with significant increases in stress response and energy supply. In conclusion, this comprehensive proteomic study identified many potentially new plastid-localized proteins and provides insights into the potential developmental and molecular mechanisms underlying chromoplast biogenesis, differentiation, and senescence in sweet orange flesh.</p></abstract><counts><page-count count="18"></page-count></counts><custom-meta-group><custom-meta><meta-name> DJS Export </meta-name><meta-value>v1</meta-value></custom-meta></custom-meta-group></article-meta><notes><glossary><title>Glossary</title><def-list><def-item><term id="term1">iTRAQ</term><def id="def1"><p>isobaric tag for relative and absolute quantitation</p></def></def-item><def-item><term id="term2">TEM</term><def id="def2"><p>transmission electron microscopy</p></def></def-item><def-item><term id="term3">IMG</term><def id="def3"><p>immature green</p></def></def-item><def-item><term id="term4">MG</term><def id="def4"><p>mature green</p></def></def-item><def-item><term id="term5">Br</term><def id="def5"><p>breaker</p></def></def-item><def-item><term id="term6">FMa</term><def id="def6"><p>full maturation</p></def></def-item><def-item><term id="term7">Ma</term><def id="def7"><p>maturation</p></def></def-item><def-item><term id="term8">OMa</term><def id="def8"><p>overmaturation</p></def></def-item><def-item><term id="term9">MEP</term><def id="def9"><p>plastidic 2-<italic>C</italic>-methyl-<sc>d</sc>-erythritol 4-phosphate</p></def></def-item><def-item><term id="term10">DAF</term><def id="def10"><p>days after flowering</p></def></def-item><def-item><term id="term11">Clp</term><def id="def11"><p>caseinolytic peptidase</p></def></def-item><def-item><term id="term12">DTT</term><def id="def12"><p>dithiothreitol</p></def></def-item><def-item><term id="term13">LC-MS/MS</term><def id="def13"><p>liquid chromatography-tandem mass spectrometry</p></def></def-item><def-item><term id="term14">ACN</term><def id="def14"><p>acetonitrile</p></def></def-item><def-item><term id="term15"><italic>m/z</italic></term><def id="def15"><p>mass-to-charge ratio</p></def></def-item></def-list></glossary></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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