Larvicidal Activity of Citrus Limonoids against Aedes albopictus Larvae
Identifieur interne : 000C75 ( Pmc/Checkpoint ); précédent : 000C74; suivant : 000C76Larvicidal Activity of Citrus Limonoids against Aedes albopictus Larvae
Auteurs : Hazrat Bilal ; Waseem Akram ; Soaib Ali-HassanSource :
- Journal of Arthropod-Borne Diseases [ 2322-1984 ] ; 2012.
Abstract
Development of insecticide resistance occurred due to the continuous and misuse of synthetic insecticides therefore, the recent study was conducted to explore eco-friendly plant extracts that have some potential to suppress mosquito larval population.
WHO recommended mosquito larval bioassay method for insecticide was used while for the analysis of citrus oils for limonin and nomilin content HPLC was used.
Among the two citrus cultivars tested as larvicide against
Valencia late showed best results in term of LC50, LT50 and percentage mortality against
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PubMed: 23378967
PubMed Central: 3547300
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Larvicidal Activity of Citrus Limonoids against <italic>Aedes albopictus</italic>
Larvae</title>
<author><name sortKey="Bilal, Hazrat" sort="Bilal, Hazrat" uniqKey="Bilal H" first="Hazrat" last="Bilal">Hazrat Bilal</name>
<affiliation><nlm:aff id="af1-jad-6-104">Medical Entomology and Disease Vector Control, Health Services Academy, Islamabad-Pakistan</nlm:aff>
<wicri:noCountry code="subfield">Islamabad-Pakistan</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Akram, Waseem" sort="Akram, Waseem" uniqKey="Akram W" first="Waseem" last="Akram">Waseem Akram</name>
<affiliation><nlm:aff id="af2-jad-6-104">Department of Agricultural Entomology, University of Agriculture Faisalabad-Pakistan</nlm:aff>
<wicri:noCountry code="subfield">University of Agriculture Faisalabad-Pakistan</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Ali Hassan, Soaib" sort="Ali Hassan, Soaib" uniqKey="Ali Hassan S" first="Soaib" last="Ali-Hassan">Soaib Ali-Hassan</name>
<affiliation><nlm:aff id="af1-jad-6-104">Medical Entomology and Disease Vector Control, Health Services Academy, Islamabad-Pakistan</nlm:aff>
<wicri:noCountry code="subfield">Islamabad-Pakistan</wicri:noCountry>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Larvicidal Activity of Citrus Limonoids against <italic>Aedes albopictus</italic>
Larvae</title>
<author><name sortKey="Bilal, Hazrat" sort="Bilal, Hazrat" uniqKey="Bilal H" first="Hazrat" last="Bilal">Hazrat Bilal</name>
<affiliation><nlm:aff id="af1-jad-6-104">Medical Entomology and Disease Vector Control, Health Services Academy, Islamabad-Pakistan</nlm:aff>
<wicri:noCountry code="subfield">Islamabad-Pakistan</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Akram, Waseem" sort="Akram, Waseem" uniqKey="Akram W" first="Waseem" last="Akram">Waseem Akram</name>
<affiliation><nlm:aff id="af2-jad-6-104">Department of Agricultural Entomology, University of Agriculture Faisalabad-Pakistan</nlm:aff>
<wicri:noCountry code="subfield">University of Agriculture Faisalabad-Pakistan</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Ali Hassan, Soaib" sort="Ali Hassan, Soaib" uniqKey="Ali Hassan S" first="Soaib" last="Ali-Hassan">Soaib Ali-Hassan</name>
<affiliation><nlm:aff id="af1-jad-6-104">Medical Entomology and Disease Vector Control, Health Services Academy, Islamabad-Pakistan</nlm:aff>
<wicri:noCountry code="subfield">Islamabad-Pakistan</wicri:noCountry>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Arthropod-Borne Diseases</title>
<idno type="ISSN">2322-1984</idno>
<idno type="eISSN">2322-2271</idno>
<imprint><date when="2012">2012</date>
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<front><div type="abstract" xml:lang="en"><sec><title>Background:</title>
<p>Development of insecticide resistance occurred due to the continuous and misuse of synthetic insecticides therefore, the recent study was conducted to explore eco-friendly plant extracts that have some potential to suppress mosquito larval population.</p>
</sec>
<sec><title>Methods:</title>
<p>WHO recommended mosquito larval bioassay method for insecticide was used while for the analysis of citrus oils for limonin and nomilin content HPLC was used.</p>
</sec>
<sec><title>Results:</title>
<p>Among the two citrus cultivars tested as larvicide against <italic>Aedes albopictus</italic>
, valencia late (<italic>Citrus sinensis</italic>
) was the best in terms of LC<sub>50</sub>
(297 ppm), % mortality (97%) and LT<sub>50</sub>
(18.49 hours) then freutrall early (<italic>Citrus reticulate</italic>
) with LC<sub>50</sub>
(377.4 ppm), % mortality (88%) and LT<sub>50</sub>
(31 hours), While nomilin gave lowest LC<sub>50</sub>
(121.04 ppm) than limonin (382.22 ppm) after 72 hours of exposure. Valencia late also had more limonin and nomilin (377 μg/ml and 21.19 μg/ml) than freutrall early (5.29 μg/ml and 3.89 μg/ml) respectively.</p>
</sec>
<sec><title>Conclusion:</title>
<p>Valencia late showed best results in term of LC<sub>50</sub>
, LT<sub>50</sub>
and percentage mortality against <italic>Aedes albopictus</italic>
as it has more amount of nomilin then freutrall early, however further evaluation in the field conditions is required.</p>
</sec>
</div>
</front>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Arthropod Borne Dis</journal-id>
<journal-id journal-id-type="iso-abbrev">J Arthropod Borne Dis</journal-id>
<journal-id journal-id-type="publisher-id">JAD</journal-id>
<journal-title-group><journal-title>Journal of Arthropod-Borne Diseases</journal-title>
</journal-title-group>
<issn pub-type="ppub">2322-1984</issn>
<issn pub-type="epub">2322-2271</issn>
<publisher><publisher-name>Tehran University of Medical Sciences</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">23378967</article-id>
<article-id pub-id-type="pmc">3547300</article-id>
<article-id pub-id-type="publisher-id">jad-6-104</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Larvicidal Activity of Citrus Limonoids against <italic>Aedes albopictus</italic>
Larvae</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Bilal</surname>
<given-names>Hazrat</given-names>
</name>
<xref ref-type="aff" rid="af1-jad-6-104"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Akram</surname>
<given-names>Waseem</given-names>
</name>
<xref ref-type="aff" rid="af2-jad-6-104"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ali-Hassan</surname>
<given-names>Soaib</given-names>
</name>
<xref ref-type="aff" rid="af1-jad-6-104"><sup>1</sup>
</xref>
<xref ref-type="corresp" rid="c1-jad-6-104"><sup>*</sup>
</xref>
</contrib>
</contrib-group>
<aff id="af1-jad-6-104"><label>1</label>
Medical Entomology and Disease Vector Control, Health Services Academy, Islamabad-Pakistan</aff>
<aff id="af2-jad-6-104"><label>2</label>
Department of Agricultural Entomology, University of Agriculture Faisalabad-Pakistan</aff>
<author-notes><corresp id="c1-jad-6-104"><label>*</label>
Corresponding author: Mr Soaib Ali Hassan, E-mail: <email>sohaib_hassan50@yahoo.com</email>
</corresp>
</author-notes>
<pub-date pub-type="collection"><month>12</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="epub"><day>31</day>
<month>12</month>
<year>2012</year>
</pub-date>
<volume>6</volume>
<issue>2</issue>
<fpage>104</fpage>
<lpage>111</lpage>
<history><date date-type="received"><day>16</day>
<month>11</month>
<year>2011</year>
</date>
<date date-type="accepted"><day>24</day>
<month>9</month>
<year>2012</year>
</date>
</history>
<permissions><copyright-statement>Copyright © Iranian Society of Medical Entomology & Tehran University of Medical Sciences</copyright-statement>
<copyright-year>2012</copyright-year>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial 3.0 License (CC BY-NC 3.0), which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.</license-p>
</license>
</permissions>
<abstract><sec><title>Background:</title>
<p>Development of insecticide resistance occurred due to the continuous and misuse of synthetic insecticides therefore, the recent study was conducted to explore eco-friendly plant extracts that have some potential to suppress mosquito larval population.</p>
</sec>
<sec><title>Methods:</title>
<p>WHO recommended mosquito larval bioassay method for insecticide was used while for the analysis of citrus oils for limonin and nomilin content HPLC was used.</p>
</sec>
<sec><title>Results:</title>
<p>Among the two citrus cultivars tested as larvicide against <italic>Aedes albopictus</italic>
, valencia late (<italic>Citrus sinensis</italic>
) was the best in terms of LC<sub>50</sub>
(297 ppm), % mortality (97%) and LT<sub>50</sub>
(18.49 hours) then freutrall early (<italic>Citrus reticulate</italic>
) with LC<sub>50</sub>
(377.4 ppm), % mortality (88%) and LT<sub>50</sub>
(31 hours), While nomilin gave lowest LC<sub>50</sub>
(121.04 ppm) than limonin (382.22 ppm) after 72 hours of exposure. Valencia late also had more limonin and nomilin (377 μg/ml and 21.19 μg/ml) than freutrall early (5.29 μg/ml and 3.89 μg/ml) respectively.</p>
</sec>
<sec><title>Conclusion:</title>
<p>Valencia late showed best results in term of LC<sub>50</sub>
, LT<sub>50</sub>
and percentage mortality against <italic>Aedes albopictus</italic>
as it has more amount of nomilin then freutrall early, however further evaluation in the field conditions is required.</p>
</sec>
</abstract>
<kwd-group><kwd>Limonin</kwd>
<kwd>nomilin</kwd>
<kwd>citrus</kwd>
<kwd><italic>Aedes</italic>
larvae</kwd>
</kwd-group>
</article-meta>
</front>
<floats-group><fig id="f1-jad-6-104" position="float"><label>Fig. 1</label>
<caption><p>HPLC analysis of a) Limonin, b) Nomilin, c) Freutrall early d) Valencia late</p>
</caption>
<graphic xlink:href="jad-6-104f1"></graphic>
</fig>
<fig id="f2-jad-6-104" position="float"><label>Fig. 2</label>
<caption><p>Percent mortality of Freutrall early and Valencia late against <italic>Aedes albopictus</italic>
larvae after 24, 48 and 72 hours</p>
</caption>
<graphic xlink:href="jad-6-104f2"></graphic>
</fig>
<table-wrap id="t1-jad-6-104" position="float"><label>Table 1</label>
<caption><p>Lethal concentration recorded at different concentration on two citrus cultivars, Limonin and Nomilin against larvae of <italic>Aedes albopictus</italic>
</p>
</caption>
<table frame="hsides" rules="groups"><thead><tr><th align="left" valign="top" rowspan="1" colspan="1"><bold>Citrus Cultivars</bold>
</th>
<th align="center" valign="top" rowspan="1" colspan="1"><bold>Limonin (μg/ml)</bold>
</th>
<th align="center" valign="top" rowspan="1" colspan="1"><bold>Nomilin (μg/ml)</bold>
</th>
<th align="center" valign="top" rowspan="1" colspan="1"><bold>Time</bold>
</th>
<th align="center" valign="top" rowspan="1" colspan="1"><bold>LC<sub>50</sub>
(ppm)</bold>
</th>
<th align="center" valign="top" rowspan="1" colspan="1"><bold>Slope±SE</bold>
</th>
<th align="center" valign="top" rowspan="1" colspan="1"><bold>χ<sup>2</sup>
</bold>
</th>
<th align="center" valign="top" rowspan="1" colspan="1"><bold><italic>P</italic>
</bold>
</th>
</tr>
</thead>
<tbody><tr><td align="left" valign="top" rowspan="3" colspan="1"><bold>Freutrall early</bold>
<break></break>
<bold><italic>Citrus reticulate</italic>
</bold>
</td>
<td align="center" valign="top" rowspan="1" colspan="1">5.29</td>
<td align="center" valign="top" rowspan="1" colspan="1">3.89</td>
<td align="center" valign="top" rowspan="1" colspan="1">24</td>
<td align="center" valign="top" rowspan="1" colspan="1">564.7</td>
<td align="center" valign="top" rowspan="1" colspan="1">1.17±0.17</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.44</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.97</td>
</tr>
<tr><td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1">48</td>
<td align="center" valign="top" rowspan="1" colspan="1">465.6</td>
<td align="center" valign="top" rowspan="1" colspan="1">1.06±0.16</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.60</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.96</td>
</tr>
<tr><td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1">72</td>
<td align="center" valign="top" rowspan="1" colspan="1">377.4</td>
<td align="center" valign="top" rowspan="1" colspan="1">1.31±0.17</td>
<td align="center" valign="top" rowspan="1" colspan="1">2.57</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.63</td>
</tr>
<tr><td align="left" valign="top" rowspan="3" colspan="1"><bold>Valencia Late</bold>
<break></break>
<bold><italic>Citrus sinensis</italic>
</bold>
</td>
<td align="center" valign="top" rowspan="1" colspan="1">377</td>
<td align="center" valign="top" rowspan="1" colspan="1">21.19</td>
<td align="center" valign="top" rowspan="1" colspan="1">24</td>
<td align="center" valign="top" rowspan="1" colspan="1">368</td>
<td align="center" valign="top" rowspan="1" colspan="1">1.09±0.17</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.17</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.99</td>
</tr>
<tr><td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1">48</td>
<td align="center" valign="top" rowspan="1" colspan="1">333.3</td>
<td align="center" valign="top" rowspan="1" colspan="1">1.11±0.17</td>
<td align="center" valign="top" rowspan="1" colspan="1">2.40</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.66</td>
</tr>
<tr><td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1">72</td>
<td align="center" valign="top" rowspan="1" colspan="1">297</td>
<td align="center" valign="top" rowspan="1" colspan="1">1.32±0.18</td>
<td align="center" valign="top" rowspan="1" colspan="1">8.78</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.06</td>
</tr>
<tr><td align="left" valign="top" rowspan="3" colspan="1"><bold>Limonin</bold>
</td>
<td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1">24</td>
<td align="center" valign="top" rowspan="1" colspan="1">836.70</td>
<td align="center" valign="top" rowspan="1" colspan="1">1.22±0.15</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.60</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.99</td>
</tr>
<tr><td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1">48</td>
<td align="center" valign="top" rowspan="1" colspan="1">548.01</td>
<td align="center" valign="top" rowspan="1" colspan="1">1.18±0.17</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.41</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.83</td>
</tr>
<tr><td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1">72</td>
<td align="center" valign="top" rowspan="1" colspan="1">382.22</td>
<td align="center" valign="top" rowspan="1" colspan="1">1.27±0.17</td>
<td align="center" valign="top" rowspan="1" colspan="1">1.21</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.04</td>
</tr>
<tr><td align="left" valign="top" rowspan="3" colspan="1"><bold>Nomilin</bold>
</td>
<td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1">24</td>
<td align="center" valign="top" rowspan="1" colspan="1">289.12</td>
<td align="center" valign="top" rowspan="1" colspan="1">1.30±0.18</td>
<td align="center" valign="top" rowspan="1" colspan="1">5.01</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.91</td>
</tr>
<tr><td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1">48</td>
<td align="center" valign="top" rowspan="1" colspan="1">163.28</td>
<td align="center" valign="top" rowspan="1" colspan="1">1.33±0.17</td>
<td align="center" valign="top" rowspan="1" colspan="1">3.22</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.52</td>
</tr>
<tr><td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1"></td>
<td align="center" valign="top" rowspan="1" colspan="1">72</td>
<td align="center" valign="top" rowspan="1" colspan="1">121.04</td>
<td align="center" valign="top" rowspan="1" colspan="1">1.29±0.17</td>
<td align="center" valign="top" rowspan="1" colspan="1">8.01</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.21</td>
</tr>
</tbody>
</table>
</table-wrap>
<table-wrap id="t2-jad-6-104" position="float"><label>Table 2</label>
<caption><p>Lethal time of citrus cultivars against larvae of <italic>Aedes albopictus</italic>
</p>
</caption>
<table frame="hsides" rules="groups"><thead><tr><th align="left" valign="top" rowspan="1" colspan="1"><bold>Citrus Cultivars</bold>
</th>
<th align="center" valign="top" rowspan="1" colspan="1"><bold>LT<sub>50</sub>
(hours)</bold>
</th>
<th align="center" valign="top" rowspan="1" colspan="1"><bold>Slope±SE</bold>
</th>
<th align="center" valign="top" rowspan="1" colspan="1"><bold>P</bold>
</th>
<th align="center" valign="top" rowspan="1" colspan="1"><bold>χ<sup>2</sup>
</bold>
</th>
</tr>
</thead>
<tbody><tr><td align="left" valign="top" rowspan="1" colspan="1"><bold>Feutrall early</bold>
<break></break>
<bold><italic>Citrus reticulate</italic>
</bold>
</td>
<td align="center" valign="top" rowspan="1" colspan="1">31.07</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.41± 0.06</td>
<td align="center" valign="top" rowspan="1" colspan="1">2.16</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.14</td>
</tr>
<tr><td align="left" valign="top" rowspan="1" colspan="1"><bold>Valencia Late</bold>
<break></break>
<bold><italic>Citrus sinensis</italic>
</bold>
</td>
<td align="center" valign="top" rowspan="1" colspan="1">18.49</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.72± 0.07</td>
<td align="center" valign="top" rowspan="1" colspan="1">7.92</td>
<td align="center" valign="top" rowspan="1" colspan="1">0.005</td>
</tr>
</tbody>
</table>
</table-wrap>
</floats-group>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Akram, Waseem" sort="Akram, Waseem" uniqKey="Akram W" first="Waseem" last="Akram">Waseem Akram</name>
<name sortKey="Ali Hassan, Soaib" sort="Ali Hassan, Soaib" uniqKey="Ali Hassan S" first="Soaib" last="Ali-Hassan">Soaib Ali-Hassan</name>
<name sortKey="Bilal, Hazrat" sort="Bilal, Hazrat" uniqKey="Bilal H" first="Hazrat" last="Bilal">Hazrat Bilal</name>
</noCountry>
</tree>
</affiliations>
</record>
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