KatG, the Bifunctional Catalase of Xanthomonas citri subsp. citri, Responds to Hydrogen Peroxide and Contributes to Epiphytic Survival on Citrus Leaves
Identifieur interne : 000125 ( Pmc/Checkpoint ); précédent : 000124; suivant : 000126KatG, the Bifunctional Catalase of Xanthomonas citri subsp. citri, Responds to Hydrogen Peroxide and Contributes to Epiphytic Survival on Citrus Leaves
Auteurs : María Laura Tondo [Argentine] ; María Laura Delprato [Argentine] ; Ivana Kraiselburd [Argentine] ; María Ver Nica Fernández Zenoff [Argentine] ; María Eugenia Farías [Argentine] ; Elena G. Orellano [Argentine]Source :
- PLoS ONE [ 1932-6203 ] ; 2016.
Abstract
Url:
DOI: 10.1371/journal.pone.0151657
PubMed: 26990197
PubMed Central: 4807922
Affiliations:
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PMC:4807922Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">KatG, the Bifunctional Catalase of <italic>Xanthomonas citri</italic> subsp. citri, Responds to Hydrogen Peroxide and Contributes to Epiphytic Survival on Citrus Leaves</title><author><name sortKey="Tondo, Maria Laura" sort="Tondo, Maria Laura" uniqKey="Tondo M" first="María Laura" last="Tondo">María Laura Tondo</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe, Argentina</addr-line></nlm:aff><country xml:lang="fr">Argentine</country><wicri:regionArea>Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe</wicri:regionArea><wicri:noRegion>Santa Fe</wicri:noRegion></affiliation></author><author><name sortKey="Delprato, Maria Laura" sort="Delprato, Maria Laura" uniqKey="Delprato M" first="María Laura" last="Delprato">María Laura Delprato</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe, Argentina</addr-line></nlm:aff><country xml:lang="fr">Argentine</country><wicri:regionArea>Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe</wicri:regionArea><wicri:noRegion>Santa Fe</wicri:noRegion></affiliation></author><author><name sortKey="Kraiselburd, Ivana" sort="Kraiselburd, Ivana" uniqKey="Kraiselburd I" first="Ivana" last="Kraiselburd">Ivana Kraiselburd</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe, Argentina</addr-line></nlm:aff><country xml:lang="fr">Argentine</country><wicri:regionArea>Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe</wicri:regionArea><wicri:noRegion>Santa Fe</wicri:noRegion></affiliation></author><author><name sortKey="Fernandez Zenoff, Maria Ver Nica" sort="Fernandez Zenoff, Maria Ver Nica" uniqKey="Fernandez Zenoff M" first="María Ver Nica" last="Fernández Zenoff">María Ver Nica Fernández Zenoff</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Planta Piloto de Procesos Industriales Microbiológicos, Consejo Nacional de Investigaciones Científicas y Técnicas, San Miguel de Tucumán, Tucumán, Argentina</addr-line></nlm:aff><country xml:lang="fr">Argentine</country><wicri:regionArea>Planta Piloto de Procesos Industriales Microbiológicos, Consejo Nacional de Investigaciones Científicas y Técnicas, San Miguel de Tucumán, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author><author><name sortKey="Farias, Maria Eugenia" sort="Farias, Maria Eugenia" uniqKey="Farias M" first="María Eugenia" last="Farías">María Eugenia Farías</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Planta Piloto de Procesos Industriales Microbiológicos, Consejo Nacional de Investigaciones Científicas y Técnicas, San Miguel de Tucumán, Tucumán, Argentina</addr-line></nlm:aff><country xml:lang="fr">Argentine</country><wicri:regionArea>Planta Piloto de Procesos Industriales Microbiológicos, Consejo Nacional de Investigaciones Científicas y Técnicas, San Miguel de Tucumán, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author><author><name sortKey="Orellano, Elena G" sort="Orellano, Elena G" uniqKey="Orellano E" first="Elena G." last="Orellano">Elena G. Orellano</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe, Argentina</addr-line></nlm:aff><country xml:lang="fr">Argentine</country><wicri:regionArea>Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe</wicri:regionArea><wicri:noRegion>Santa Fe</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26990197</idno><idno type="pmc">4807922</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4807922</idno><idno type="RBID">PMC:4807922</idno><idno type="doi">10.1371/journal.pone.0151657</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000385</idno><idno type="wicri:Area/Pmc/Curation">000384</idno><idno type="wicri:Area/Pmc/Checkpoint">000125</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">KatG, the Bifunctional Catalase of <italic>Xanthomonas citri</italic> subsp. citri, Responds to Hydrogen Peroxide and Contributes to Epiphytic Survival on Citrus Leaves</title><author><name sortKey="Tondo, Maria Laura" sort="Tondo, Maria Laura" uniqKey="Tondo M" first="María Laura" last="Tondo">María Laura Tondo</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe, Argentina</addr-line></nlm:aff><country xml:lang="fr">Argentine</country><wicri:regionArea>Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe</wicri:regionArea><wicri:noRegion>Santa Fe</wicri:noRegion></affiliation></author><author><name sortKey="Delprato, Maria Laura" sort="Delprato, Maria Laura" uniqKey="Delprato M" first="María Laura" last="Delprato">María Laura Delprato</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe, Argentina</addr-line></nlm:aff><country xml:lang="fr">Argentine</country><wicri:regionArea>Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe</wicri:regionArea><wicri:noRegion>Santa Fe</wicri:noRegion></affiliation></author><author><name sortKey="Kraiselburd, Ivana" sort="Kraiselburd, Ivana" uniqKey="Kraiselburd I" first="Ivana" last="Kraiselburd">Ivana Kraiselburd</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe, Argentina</addr-line></nlm:aff><country xml:lang="fr">Argentine</country><wicri:regionArea>Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe</wicri:regionArea><wicri:noRegion>Santa Fe</wicri:noRegion></affiliation></author><author><name sortKey="Fernandez Zenoff, Maria Ver Nica" sort="Fernandez Zenoff, Maria Ver Nica" uniqKey="Fernandez Zenoff M" first="María Ver Nica" last="Fernández Zenoff">María Ver Nica Fernández Zenoff</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Planta Piloto de Procesos Industriales Microbiológicos, Consejo Nacional de Investigaciones Científicas y Técnicas, San Miguel de Tucumán, Tucumán, Argentina</addr-line></nlm:aff><country xml:lang="fr">Argentine</country><wicri:regionArea>Planta Piloto de Procesos Industriales Microbiológicos, Consejo Nacional de Investigaciones Científicas y Técnicas, San Miguel de Tucumán, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author><author><name sortKey="Farias, Maria Eugenia" sort="Farias, Maria Eugenia" uniqKey="Farias M" first="María Eugenia" last="Farías">María Eugenia Farías</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Planta Piloto de Procesos Industriales Microbiológicos, Consejo Nacional de Investigaciones Científicas y Técnicas, San Miguel de Tucumán, Tucumán, Argentina</addr-line></nlm:aff><country xml:lang="fr">Argentine</country><wicri:regionArea>Planta Piloto de Procesos Industriales Microbiológicos, Consejo Nacional de Investigaciones Científicas y Técnicas, San Miguel de Tucumán, Tucumán</wicri:regionArea><wicri:noRegion>Tucumán</wicri:noRegion></affiliation></author><author><name sortKey="Orellano, Elena G" sort="Orellano, Elena G" uniqKey="Orellano E" first="Elena G." last="Orellano">Elena G. Orellano</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe, Argentina</addr-line></nlm:aff><country xml:lang="fr">Argentine</country><wicri:regionArea>Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe</wicri:regionArea><wicri:noRegion>Santa Fe</wicri:noRegion></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><italic>Xanthomonas citri</italic> subsp. citri (Xcc) is the bacterium responsible for citrus canker. This bacterium is exposed to reactive oxygen species (ROS) at different points during its life cycle, including those normally produced by aerobic respiration or upon exposition to ultraviolet (UV) radiation. Moreover, ROS are key components of the host immune response. Among enzymatic ROS-detoxifying mechanisms, catalases eliminate H<sub>2</sub>O<sub>2</sub>, avoiding the potential damage caused by this specie. Xcc genome includes four catalase genes. In this work, we studied the physiological role of KatG, the only bifunctional catalase of Xcc, through the construction and characterization of a modified strain (Xcc<italic>katG</italic>), carrying an insertional mutation in the <italic>katG</italic> gene. First, we evaluated the involvement of KatG in the bacterial adaptive response to H<sub>2</sub>O<sub>2</sub>. Xcc<italic>katG</italic> cultures exhibited lower catalase activity than those of the wild-type strain, and this activity was not induced upon treatment with sub-lethal doses of H<sub>2</sub>O<sub>2</sub>. Moreover, the KatG-deficient mutant exhibited decreased tolerance to H<sub>2</sub>O<sub>2</sub> toxicity compared to wild-type cells and accumulated high intracellular levels of peroxides upon exposure to sub-lethal concentrations of H<sub>2</sub>O<sub>2</sub>. To further study the role of KatG in Xcc physiology, we evaluated bacterial survival upon exposure to UV-A or UV-B radiation. In both conditions, Xcc<italic>katG</italic> showed a high mortality in comparison to Xcc wild-type. Finally, we studied the development of bacterial biofilms. While structured biofilms were observed for the Xcc wild-type, the development of these structures was impaired for Xcc<italic>katG</italic>. Based on these results, we demonstrated that KatG is responsible for Xcc adaptive response to H<sub>2</sub>O<sub>2</sub> and a key component of the bacterial response to oxidative stress. Moreover, this enzyme plays an important role during Xcc epiphytic survival, being essential for biofilm formation and UV resistance.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brunings, Am" uniqKey="Brunings A">AM Brunings</name></author><author><name sortKey="Gabriel, Dw" uniqKey="Gabriel D">DW Gabriel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Melotto, M" uniqKey="Melotto M">M Melotto</name></author><author><name sortKey="Underwood, W" uniqKey="Underwood W">W Underwood</name></author><author><name sortKey="He, Sy" uniqKey="He 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, CA USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26990197</article-id><article-id pub-id-type="pmc">4807922</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0151657</article-id><article-id pub-id-type="publisher-id">PONE-D-15-48002</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Enzymology</subject><subj-group><subject>Enzymes</subject><subj-group><subject>Catalases</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Proteins</subject><subj-group><subject>Enzymes</subject><subj-group><subject>Catalases</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Microbiology</subject><subj-group><subject>Bacteriology</subject><subj-group><subject>Bacterial Biofilms</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Microbiology</subject><subj-group><subject>Biofilms</subject><subj-group><subject>Bacterial Biofilms</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Anatomy</subject><subj-group><subject>Leaves</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Physical Sciences</subject><subj-group><subject>Physics</subject><subj-group><subject>Electromagnetic Radiation</subject><subj-group><subject>Light</subject><subj-group><subject>Ultraviolet Radiation</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Physical Sciences</subject><subj-group><subject>Chemistry</subject><subj-group><subject>Chemical Compounds</subject><subj-group><subject>Oxides</subject><subj-group><subject>Peroxides</subject><subj-group><subject>Hydrogen Peroxide</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Physical sciences</subject><subj-group><subject>Physics</subject><subj-group><subject>Electromagnetic radiation</subject><subj-group><subject>Light</subject><subj-group><subject>Ultraviolet radiation</subject><subj-group><subject>Ultraviolet A</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Oxidative Stress</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Physical sciences</subject><subj-group><subject>Physics</subject><subj-group><subject>Electromagnetic radiation</subject><subj-group><subject>Light</subject><subj-group><subject>Ultraviolet radiation</subject><subj-group><subject>Ultraviolet B</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></article-categories><title-group><article-title>KatG, the Bifunctional Catalase of <italic>Xanthomonas citri</italic> subsp. citri, Responds to Hydrogen Peroxide and Contributes to Epiphytic Survival on Citrus Leaves</article-title><alt-title alt-title-type="running-head">Role of KatG in <italic>Xanthomonas citri</italic> subsp. citri</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Tondo</surname><given-names>María Laura</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Delprato</surname><given-names>María Laura</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kraiselburd</surname><given-names>Ivana</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Fernández Zenoff</surname><given-names>María Verónica</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Farías</surname><given-names>María Eugenia</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Orellano</surname><given-names>Elena G.</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="corresp" rid="cor001">*</xref></contrib></contrib-group><aff id="aff001"><label>1</label><addr-line>Molecular Biology Division, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe, Argentina</addr-line></aff><aff id="aff002"><label>2</label><addr-line>Planta Piloto de Procesos Industriales Microbiológicos, Consejo Nacional de Investigaciones Científicas y Técnicas, San Miguel de Tucumán, Tucumán, Argentina</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Roop</surname><given-names>Roy Martin</given-names><suffix>II</suffix></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>East Carolina University School of Medicine, UNITED STATES</addr-line></aff><author-notes><fn fn-type="conflict" id="coi001"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con" id="contrib001"><p>Conceived and designed the experiments: MLT EGO. Performed the experiments: MLT MLD IK MVFZ. Analyzed the data: MLT MLD IK MVFZ MEF EGO. Contributed reagents/materials/analysis tools: MEF EGO. Wrote the paper: MLT MLD IK EGO.</p></fn><corresp id="cor001">* E-mail: <email>orellano@ibr-conicet.gov.ar</email></corresp></author-notes><pub-date pub-type="epub"><day>18</day><month>3</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>11</volume><issue>3</issue><elocation-id>e0151657</elocation-id><history><date date-type="received"><day>2</day><month>11</month><year>2015</year></date><date date-type="accepted"><day>2</day><month>3</month><year>2016</year></date></history><permissions><copyright-statement>© 2016 Tondo et al</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>Tondo et al</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><self-uri content-type="pdf" xlink:type="simple" xlink:href="pone.0151657.pdf"></self-uri><abstract><p><italic>Xanthomonas citri</italic> subsp. citri (Xcc) is the bacterium responsible for citrus canker. This bacterium is exposed to reactive oxygen species (ROS) at different points during its life cycle, including those normally produced by aerobic respiration or upon exposition to ultraviolet (UV) radiation. Moreover, ROS are key components of the host immune response. Among enzymatic ROS-detoxifying mechanisms, catalases eliminate H<sub>2</sub>O<sub>2</sub>, avoiding the potential damage caused by this specie. Xcc genome includes four catalase genes. In this work, we studied the physiological role of KatG, the only bifunctional catalase of Xcc, through the construction and characterization of a modified strain (Xcc<italic>katG</italic>), carrying an insertional mutation in the <italic>katG</italic> gene. First, we evaluated the involvement of KatG in the bacterial adaptive response to H<sub>2</sub>O<sub>2</sub>. Xcc<italic>katG</italic> cultures exhibited lower catalase activity than those of the wild-type strain, and this activity was not induced upon treatment with sub-lethal doses of H<sub>2</sub>O<sub>2</sub>. Moreover, the KatG-deficient mutant exhibited decreased tolerance to H<sub>2</sub>O<sub>2</sub> toxicity compared to wild-type cells and accumulated high intracellular levels of peroxides upon exposure to sub-lethal concentrations of H<sub>2</sub>O<sub>2</sub>. To further study the role of KatG in Xcc physiology, we evaluated bacterial survival upon exposure to UV-A or UV-B radiation. In both conditions, Xcc<italic>katG</italic> showed a high mortality in comparison to Xcc wild-type. Finally, we studied the development of bacterial biofilms. While structured biofilms were observed for the Xcc wild-type, the development of these structures was impaired for Xcc<italic>katG</italic>. Based on these results, we demonstrated that KatG is responsible for Xcc adaptive response to H<sub>2</sub>O<sub>2</sub> and a key component of the bacterial response to oxidative stress. Moreover, this enzyme plays an important role during Xcc epiphytic survival, being essential for biofilm formation and UV resistance.</p></abstract><funding-group><funding-statement>This work was supported by Agencia Nacional de Promoción Científica y Tecnológica, PICT 2010-1762 to EGO (<ext-link ext-link-type="uri" xlink:href="http://www.agencia.mincyt.gob.ar/">http://www.agencia.mincyt.gob.ar/</ext-link>), and Consejo Nacional de Investigaciones Científicas y Técnicas, PIP 00873 to EGO (<ext-link ext-link-type="uri" xlink:href="http://www.conicet.gov.ar/">http://www.conicet.gov.ar/</ext-link>). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><fig-count count="6"></fig-count><table-count count="2"></table-count><page-count count="19"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>All relevant data are within the paper and its Supporting Information file.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>All relevant data are within the paper and its Supporting Information file.</p></notes></front></pmc><affiliations><list><country><li>Argentine</li></country></list><tree><country name="Argentine"><noRegion><name sortKey="Tondo, Maria Laura" sort="Tondo, Maria Laura" uniqKey="Tondo M" first="María Laura" last="Tondo">María Laura Tondo</name></noRegion><name sortKey="Delprato, Maria Laura" sort="Delprato, Maria Laura" uniqKey="Delprato M" first="María Laura" last="Delprato">María Laura Delprato</name><name sortKey="Farias, Maria Eugenia" sort="Farias, Maria Eugenia" uniqKey="Farias M" first="María Eugenia" last="Farías">María Eugenia Farías</name><name sortKey="Fernandez Zenoff, Maria Ver Nica" sort="Fernandez Zenoff, Maria Ver Nica" uniqKey="Fernandez Zenoff M" first="María Ver Nica" last="Fernández Zenoff">María Ver Nica Fernández Zenoff</name><name sortKey="Kraiselburd, Ivana" sort="Kraiselburd, Ivana" uniqKey="Kraiselburd I" first="Ivana" last="Kraiselburd">Ivana Kraiselburd</name><name sortKey="Orellano, Elena G" sort="Orellano, Elena G" uniqKey="Orellano E" first="Elena G." last="Orellano">Elena G. 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