OrangerV1, PascalFrancis, Checkpoint, bibRecord, 000842

Purification and characterization of a beta-glucosidase from Citrus sinensis var. valencia fruit tissue

Identifieur interne : 000842 ( PascalFrancis/Checkpoint ); précédent : 000841; suivant : 000843

Purification and characterization of a beta-glucosidase from Citrus sinensis var. valencia fruit tissue

Auteurs : Randall G. Cameron [États-Unis] ; John A. Manthey [États-Unis] ; Robert A. Baker [États-Unis] ; Karel Grohmann [États-Unis]

Source :

Journal of agricultural and food chemistry : (Print) [ 0021-8561 ] ; 2001.

RBID : Pascal:02-0002042

Descripteurs français

Pascal (Inist)
- Purification, Caractérisation, β-Glucosidase, Spécificité tissu, Fruit, Activité enzymatique, Spécificité substrat, Activité catalytique, pH optimum, Citrus sinensis, Masse moléculaire, Jus d'orange, Sous produit.
Wicri :
- topic : Fruit.

English descriptors

KwdEn :
- By product, Catalyst activity, Characterization, Citrus sinensis, Enzymatic activity, Fruit, Molecular mass, Optimum pH, Orange juice, Purification, Substrate specificity, Tissue specificity, β-Glucosidase.

Abstract

A preliminary survey demonstrated activity for α-D-glucosidase, α-D-mannosidase, α-L-arabinosidase, β-D-glucosidase, β-D-xylosidase, and β-D-galactosidase in orange fruit flavedo and albedo tissue, α-L-Rhamnosidase was not detected. Subsequently, a β-glucosidase was purified from mature fruit rag tissue (composed of intersegmental septa, squeezed juice sacs, and fruit core tissue) of Citrus sinensis var. Valencia. The β-glucosidase exhibited low levels of activity against p-nitrophenyl-β-D-fucopyranoside (13.5%) and p-nitrophenyl-a-D-glucopyranoside (7.0%), compared to its activity against p-nitrophenyl-β-D-glucopyranoside (pNPG, 100%). The enzyme was purified by a combination of ion exchange (anion and cation) and gel filtration (Superdex and Toyopearl HW-55S) chromatography. It has an apparent molecular mass of 64 kDa by denaturing electrophoresis or 55 kDa by gel filtration chromatography (BioGel P-100). Hydrolysis of pNPG demonstrated a pH optimum between 4.5 and 5.5. At pH 5.0 the temperature optimum was 40 °C. At pH 5.0 and 40 °C the K_m forpNPG was 0.1146 mM and it had a V_max of 5.2792 nkatal.mg^-1 protein (katal = 0.06 International Units = the amount of enzyme that produces, under standard conditions, one μmol of product per min). Of the substrates tested, the enzyme was most active against the disaccharide cellobiose (1⇒4), but was not active against p-nitrophenyl-β-D-cellobioside. High levels of activity also were observed with the disaccharides laminaribiose (1⇒3), gentiobiose (1⇒6), and sophorose (1⇒2). Activity greater than that observed with pNPG was obtained with the flavonoids hesperetin-7-glucoside and prunin (naringenin-7-glucoside), salicin, mandelonitrile-β-D-glucoside (a cyanogenic substrate), and sinigrin (a glucosinolate). The enzyme was not active against amygdalin, coniferin, or limonin glucoside.

Affiliations:

États-Unis

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Le document en format XML

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<front><div type="abstract" xml:lang="en">A preliminary survey demonstrated activity for α-D-glucosidase, α-D-mannosidase, α-L-arabinosidase, β-D-glucosidase, β-D-xylosidase, and β-D-galactosidase in orange fruit flavedo and albedo tissue, α-L-Rhamnosidase was not detected. Subsequently, a β-glucosidase was purified from mature fruit rag tissue (composed of intersegmental septa, squeezed juice sacs, and fruit core tissue) of Citrus sinensis var. Valencia. The β-glucosidase exhibited low levels of activity against p-nitrophenyl-β-D-fucopyranoside (13.5%) and p-nitrophenyl-a-D-glucopyranoside (7.0%), compared to its activity against p-nitrophenyl-β-D-glucopyranoside (pNPG, 100%). The enzyme was purified by a combination of ion exchange (anion and cation) and gel filtration (Superdex and Toyopearl HW-55S) chromatography. It has an apparent molecular mass of 64 kDa by denaturing electrophoresis or 55 kDa by gel filtration chromatography (BioGel P-100). Hydrolysis of pNPG demonstrated a pH optimum between 4.5 and 5.5. At pH 5.0 the temperature optimum was 40 °C. At pH 5.0 and 40 °C the K<sub>m</sub>
 forpNPG was 0.1146 mM and it had a V<sub>max</sub>
 of 5.2792 nkatal.mg<sup>-1</sup>
 protein (katal = 0.06 International Units = the amount of enzyme that produces, under standard conditions, one μmol of product per min). Of the substrates tested, the enzyme was most active against the disaccharide cellobiose (1⇒4), but was not active against p-nitrophenyl-β-D-cellobioside. High levels of activity also were observed with the disaccharides laminaribiose (1⇒3), gentiobiose (1⇒6), and sophorose (1⇒2). Activity greater than that observed with pNPG was obtained with the flavonoids hesperetin-7-glucoside and prunin (naringenin-7-glucoside), salicin, mandelonitrile-β-D-glucoside (a cyanogenic substrate), and sinigrin (a glucosinolate). The enzyme was not active against amygdalin, coniferin, or limonin glucoside.</div>
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<fC01 i1="01" l="ENG"><s0>A preliminary survey demonstrated activity for α-D-glucosidase, α-D-mannosidase, α-L-arabinosidase, β-D-glucosidase, β-D-xylosidase, and β-D-galactosidase in orange fruit flavedo and albedo tissue, α-L-Rhamnosidase was not detected. Subsequently, a β-glucosidase was purified from mature fruit rag tissue (composed of intersegmental septa, squeezed juice sacs, and fruit core tissue) of Citrus sinensis var. Valencia. The β-glucosidase exhibited low levels of activity against p-nitrophenyl-β-D-fucopyranoside (13.5%) and p-nitrophenyl-a-D-glucopyranoside (7.0%), compared to its activity against p-nitrophenyl-β-D-glucopyranoside (pNPG, 100%). The enzyme was purified by a combination of ion exchange (anion and cation) and gel filtration (Superdex and Toyopearl HW-55S) chromatography. It has an apparent molecular mass of 64 kDa by denaturing electrophoresis or 55 kDa by gel filtration chromatography (BioGel P-100). Hydrolysis of pNPG demonstrated a pH optimum between 4.5 and 5.5. At pH 5.0 the temperature optimum was 40 °C. At pH 5.0 and 40 °C the K<sub>m</sub>
 forpNPG was 0.1146 mM and it had a V<sub>max</sub>
 of 5.2792 nkatal.mg<sup>-1</sup>
 protein (katal = 0.06 International Units = the amount of enzyme that produces, under standard conditions, one μmol of product per min). Of the substrates tested, the enzyme was most active against the disaccharide cellobiose (1⇒4), but was not active against p-nitrophenyl-β-D-cellobioside. High levels of activity also were observed with the disaccharides laminaribiose (1⇒3), gentiobiose (1⇒6), and sophorose (1⇒2). Activity greater than that observed with pNPG was obtained with the flavonoids hesperetin-7-glucoside and prunin (naringenin-7-glucoside), salicin, mandelonitrile-β-D-glucoside (a cyanogenic substrate), and sinigrin (a glucosinolate). The enzyme was not active against amygdalin, coniferin, or limonin glucoside.</s0>
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<fC03 i1="01" i2="X" l="ENG"><s0>Purification</s0>
<s5>01</s5>
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<fC03 i1="01" i2="X" l="SPA"><s0>Purificación</s0>
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<s5>02</s5>
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<s5>02</s5>
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<fC03 i1="02" i2="X" l="SPA"><s0>Caracterización</s0>
<s5>02</s5>
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<fC03 i1="03" i2="X" l="FRE"><s0>β-Glucosidase</s0>
<s2>FE</s2>
<s5>03</s5>
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<fC03 i1="03" i2="X" l="ENG"><s0>β-Glucosidase</s0>
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<s5>03</s5>
<s6>«β»-Glucosidase</s6>
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<fC03 i1="03" i2="X" l="SPA"><s0>β-Glucosidase</s0>
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<s5>03</s5>
<s6>«β»-Glucosidase</s6>
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<s5>04</s5>
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<s5>04</s5>
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<s5>04</s5>
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<s5>06</s5>
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<fC03 i1="06" i2="X" l="SPA"><s0>Actividad enzimática</s0>
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<s5>07</s5>
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<s5>07</s5>
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<fC03 i1="07" i2="X" l="SPA"><s0>Especificidad sustrato</s0>
<s5>07</s5>
</fC03>
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<s5>08</s5>
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<s5>08</s5>
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<fC03 i1="08" i2="X" l="SPA"><s0>Actividad catalítica</s0>
<s5>08</s5>
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<fC03 i1="09" i2="X" l="FRE"><s0>pH optimum</s0>
<s5>09</s5>
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<fC03 i1="09" i2="X" l="ENG"><s0>Optimum pH</s0>
<s5>09</s5>
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<fC03 i1="09" i2="X" l="SPA"><s0>pH óptimo</s0>
<s5>09</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>10</s5>
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<fC03 i1="10" i2="X" l="SPA"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>10</s5>
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<fC03 i1="11" i2="X" l="FRE"><s0>Masse moléculaire</s0>
<s5>33</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Molecular mass</s0>
<s5>33</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Masa molecular</s0>
<s5>33</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Jus d'orange</s0>
<s5>34</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Orange juice</s0>
<s5>34</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Zugo naranja</s0>
<s5>34</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Sous produit</s0>
<s5>35</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>By product</s0>
<s5>35</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Subproducto</s0>
<s5>35</s5>
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<fC07 i1="01" i2="X" l="FRE"><s0>O-Glycosidases</s0>
<s2>FE</s2>
<s6>«O»-Glycosidases</s6>
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<s2>FE</s2>
<s6>«O»-Glycosidases</s6>
</fC07>
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<s2>FE</s2>
<s6>«O»-Glycosidases</s6>
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<fC07 i1="02" i2="X" l="FRE"><s0>Glycosidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Glycosidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Glycosidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Enzyme</s0>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Enzyme</s0>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Enzima</s0>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Agrume</s0>
<s5>39</s5>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Citrus fruit</s0>
<s5>39</s5>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Agrios</s0>
<s5>39</s5>
</fC07>
<fC07 i1="10" i2="X" l="FRE"><s0>Arbre fruitier</s0>
<s5>40</s5>
</fC07>
<fC07 i1="10" i2="X" l="ENG"><s0>Fruit tree</s0>
<s5>40</s5>
</fC07>
<fC07 i1="10" i2="X" l="SPA"><s0>Arbol frutal</s0>
<s5>40</s5>
</fC07>
<fN21><s1>001</s1>
</fN21>
</pA>
</standard>
</inist>
<affiliations><list><country><li>États-Unis</li>
</country>
</list>
<tree><country name="États-Unis"><noRegion><name sortKey="Cameron, Randall G" sort="Cameron, Randall G" uniqKey="Cameron R" first="Randall G." last="Cameron">Randall G. Cameron</name>
</noRegion>
<name sortKey="Baker, Robert A" sort="Baker, Robert A" uniqKey="Baker R" first="Robert A." last="Baker">Robert A. Baker</name>
<name sortKey="Grohmann, Karel" sort="Grohmann, Karel" uniqKey="Grohmann K" first="Karel" last="Grohmann">Karel Grohmann</name>
<name sortKey="Manthey, John A" sort="Manthey, John A" uniqKey="Manthey J" first="John A." last="Manthey">John A. Manthey</name>
</country>
</tree>
</affiliations>
</record>

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Purification and characterization of a beta-glucosidase from Citrus sinensis var. valencia fruit tissue

Purification and characterization of a beta-glucosidase from Citrus sinensis var. valencia fruit tissue

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