Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification.
Identifieur interne : 001491 ( Ncbi/Merge ); précédent : 001490; suivant : 001492Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification.
Auteurs : A. Sakai [Japon] ; S. Kobayashi ; I. OiyamaSource :
- Plant cell reports [ 0721-7714 ] ; 1990.
Abstract
The nucellar cells of navel orange(Citrus sinensis Osb. var. brasiliensis Tanaka) were successfully cryopreserved by vitrification. In this method, cells were sufficiently dehydrated with highly concentrated cryoprotective solution(PVS2) prior to direct plunge in liquid nitrogen. The PVS2 contains(w/v) 30% glycerol, 15% ethylene glycol and 15% DMSO in Murashige-Tucker medium(MT) containing 0.15 M sucrose. Cells were treated with 60% PVS2 at 25°C for 5 min and then chilled PVS2 at 0°C for 3 min. The cell suspension of about 0.1 ml was loaded in a 0.5 ml transparent plastic straw and directly plunged in liquid nitrogen for 30 min. After rapid warming, the cell suspension was expelled in 2 ml of MT medium containing 1.2 M sucrose. The average rate of survival was about 80%. The vitrified cells regenerated plantlets. This method is very simple and the time required for cryopreservation is only about 10 min.
DOI: 10.1007/BF00232130
PubMed: 24226373
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000F58
- to stream PubMed, to step Curation: 000F58
- to stream PubMed, to step Checkpoint: 000F58
Links to Exploration step
pubmed:24226373Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification.</title><author><name sortKey="Sakai, A" sort="Sakai, A" uniqKey="Sakai A" first="A" last="Sakai">A. Sakai</name><affiliation wicri:level="1"><nlm:affiliation>, Asabucho 1-5-23 Kitaku, 001, Sapporo, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>, Asabucho 1-5-23 Kitaku, 001, Sapporo</wicri:regionArea><wicri:noRegion>Sapporo</wicri:noRegion></affiliation></author><author><name sortKey="Kobayashi, S" sort="Kobayashi, S" uniqKey="Kobayashi S" first="S" last="Kobayashi">S. Kobayashi</name></author><author><name sortKey="Oiyama, I" sort="Oiyama, I" uniqKey="Oiyama I" first="I" last="Oiyama">I. Oiyama</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1990">1990</date><idno type="RBID">pubmed:24226373</idno><idno type="pmid">24226373</idno><idno type="doi">10.1007/BF00232130</idno><idno type="wicri:Area/PubMed/Corpus">000F58</idno><idno type="wicri:Area/PubMed/Curation">000F58</idno><idno type="wicri:Area/PubMed/Checkpoint">000F58</idno><idno type="wicri:Area/Ncbi/Merge">001491</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification.</title><author><name sortKey="Sakai, A" sort="Sakai, A" uniqKey="Sakai A" first="A" last="Sakai">A. Sakai</name><affiliation wicri:level="1"><nlm:affiliation>, Asabucho 1-5-23 Kitaku, 001, Sapporo, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>, Asabucho 1-5-23 Kitaku, 001, Sapporo</wicri:regionArea><wicri:noRegion>Sapporo</wicri:noRegion></affiliation></author><author><name sortKey="Kobayashi, S" sort="Kobayashi, S" uniqKey="Kobayashi S" first="S" last="Kobayashi">S. Kobayashi</name></author><author><name sortKey="Oiyama, I" sort="Oiyama, I" uniqKey="Oiyama I" first="I" last="Oiyama">I. Oiyama</name></author></analytic><series><title level="j">Plant cell reports</title><idno type="ISSN">0721-7714</idno><imprint><date when="1990" type="published">1990</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The nucellar cells of navel orange(Citrus sinensis Osb. var. brasiliensis Tanaka) were successfully cryopreserved by vitrification. In this method, cells were sufficiently dehydrated with highly concentrated cryoprotective solution(PVS2) prior to direct plunge in liquid nitrogen. The PVS2 contains(w/v) 30% glycerol, 15% ethylene glycol and 15% DMSO in Murashige-Tucker medium(MT) containing 0.15 M sucrose. Cells were treated with 60% PVS2 at 25°C for 5 min and then chilled PVS2 at 0°C for 3 min. The cell suspension of about 0.1 ml was loaded in a 0.5 ml transparent plastic straw and directly plunged in liquid nitrogen for 30 min. After rapid warming, the cell suspension was expelled in 2 ml of MT medium containing 1.2 M sucrose. The average rate of survival was about 80%. The vitrified cells regenerated plantlets. This method is very simple and the time required for cryopreservation is only about 10 min.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">24226373</PMID><DateCreated><Year>2013</Year><Month>11</Month><Day>14</Day></DateCreated><DateCompleted><Year>2013</Year><Month>11</Month><Day>15</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>14</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0721-7714</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><Issue>1</Issue><PubDate><Year>1990</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Plant cell reports</Title><ISOAbbreviation>Plant Cell Rep.</ISOAbbreviation></Journal><ArticleTitle>Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification.</ArticleTitle><Pagination><MedlinePgn>30-3</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/BF00232130</ELocationID><Abstract><AbstractText>The nucellar cells of navel orange(Citrus sinensis Osb. var. brasiliensis Tanaka) were successfully cryopreserved by vitrification. In this method, cells were sufficiently dehydrated with highly concentrated cryoprotective solution(PVS2) prior to direct plunge in liquid nitrogen. The PVS2 contains(w/v) 30% glycerol, 15% ethylene glycol and 15% DMSO in Murashige-Tucker medium(MT) containing 0.15 M sucrose. Cells were treated with 60% PVS2 at 25°C for 5 min and then chilled PVS2 at 0°C for 3 min. The cell suspension of about 0.1 ml was loaded in a 0.5 ml transparent plastic straw and directly plunged in liquid nitrogen for 30 min. After rapid warming, the cell suspension was expelled in 2 ml of MT medium containing 1.2 M sucrose. The average rate of survival was about 80%. The vitrified cells regenerated plantlets. This method is very simple and the time required for cryopreservation is only about 10 min.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sakai</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>, Asabucho 1-5-23 Kitaku, 001, Sapporo, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kobayashi</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Oiyama</LastName><ForeName>I</ForeName><Initials>I</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Plant Cell Rep</MedlineTA><NlmUniqueID>9880970</NlmUniqueID><ISSNLinking>0721-7714</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>1989</Year><Month>10</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>1990</Year><Month>1</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>11</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>1990</Year><Month>6</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1990</Year><Month>6</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24226373</ArticleId><ArticleId IdType="doi">10.1007/BF00232130</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Kobayashi, S" sort="Kobayashi, S" uniqKey="Kobayashi S" first="S" last="Kobayashi">S. Kobayashi</name><name sortKey="Oiyama, I" sort="Oiyama, I" uniqKey="Oiyama I" first="I" last="Oiyama">I. Oiyama</name></noCountry><country name="Japon"><noRegion><name sortKey="Sakai, A" sort="Sakai, A" uniqKey="Sakai A" first="A" last="Sakai">A. Sakai</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001491 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 001491 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Ncbi
|étape= Merge
|type= RBID
|clé= pubmed:24226373
|texte= Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:24226373" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |