Rapid methods for extracting and quantifying phenolic compounds in citrus rinds
Identifieur interne : 001E68 ( Ncbi/Curation ); précédent : 001E67; suivant : 001E69Rapid methods for extracting and quantifying phenolic compounds in citrus rinds
Auteurs : Lembe Samukelo Magwaza ; Umezuruike Linus Opara ; Paul J. R. Cronje ; Sandra Landahl ; Jose Ordaz Ortiz ; Leon A. TerrySource :
- Food Science & Nutrition [ 2048-7177 ] ; 2015.
Abstract
Conventional methods for extracting and quantifying phenolic compounds in citrus rinds are time consuming. Rapid methods for extracting and quantifying phenolic compounds were developed by comparing three extraction solvent combinations (80:20 v/v ethanol:H2O; 70:29.5:0.5 v/v/v methanol:H2O:
Url:
DOI: 10.1002/fsn3.210
PubMed: 26788305
PubMed Central: 4708637
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid methods for extracting and quantifying phenolic compounds in citrus rinds</title><author><name sortKey="Magwaza, Lembe Samukelo" sort="Magwaza, Lembe Samukelo" uniqKey="Magwaza L" first="Lembe Samukelo" last="Magwaza">Lembe Samukelo Magwaza</name><affiliation><nlm:aff id="fsn3210-aff-0001"></nlm:aff></affiliation></author><author><name sortKey="Opara, Umezuruike Linus" sort="Opara, Umezuruike Linus" uniqKey="Opara U" first="Umezuruike Linus" last="Opara">Umezuruike Linus Opara</name><affiliation><nlm:aff id="fsn3210-aff-0002"></nlm:aff></affiliation><affiliation><nlm:aff id="fsn3210-aff-0003"></nlm:aff></affiliation></author><author><name sortKey="Cronje, Paul J R" sort="Cronje, Paul J R" uniqKey="Cronje P" first="Paul J. R." last="Cronje">Paul J. R. Cronje</name><affiliation><nlm:aff id="fsn3210-aff-0004"></nlm:aff></affiliation></author><author><name sortKey="Landahl, Sandra" sort="Landahl, Sandra" uniqKey="Landahl S" first="Sandra" last="Landahl">Sandra Landahl</name><affiliation><nlm:aff id="fsn3210-aff-0005"></nlm:aff></affiliation></author><author><name sortKey="Ortiz, Jose Ordaz" sort="Ortiz, Jose Ordaz" uniqKey="Ortiz J" first="Jose Ordaz" last="Ortiz">Jose Ordaz Ortiz</name><affiliation><nlm:aff id="fsn3210-aff-0005"></nlm:aff></affiliation></author><author><name sortKey="Terry, Leon A" sort="Terry, Leon A" uniqKey="Terry L" first="Leon A." last="Terry">Leon A. Terry</name><affiliation><nlm:aff id="fsn3210-aff-0005"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26788305</idno><idno type="pmc">4708637</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4708637</idno><idno type="RBID">PMC:4708637</idno><idno type="doi">10.1002/fsn3.210</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000060</idno><idno type="wicri:Area/Pmc/Curation">000060</idno><idno type="wicri:Area/Pmc/Checkpoint">000368</idno><idno type="wicri:Area/Ncbi/Merge">001E68</idno><idno type="wicri:Area/Ncbi/Curation">001E68</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Rapid methods for extracting and quantifying phenolic compounds in citrus rinds</title><author><name sortKey="Magwaza, Lembe Samukelo" sort="Magwaza, Lembe Samukelo" uniqKey="Magwaza L" first="Lembe Samukelo" last="Magwaza">Lembe Samukelo Magwaza</name><affiliation><nlm:aff id="fsn3210-aff-0001"></nlm:aff></affiliation></author><author><name sortKey="Opara, Umezuruike Linus" sort="Opara, Umezuruike Linus" uniqKey="Opara U" first="Umezuruike Linus" last="Opara">Umezuruike Linus Opara</name><affiliation><nlm:aff id="fsn3210-aff-0002"></nlm:aff></affiliation><affiliation><nlm:aff id="fsn3210-aff-0003"></nlm:aff></affiliation></author><author><name sortKey="Cronje, Paul J R" sort="Cronje, Paul J R" uniqKey="Cronje P" first="Paul J. R." last="Cronje">Paul J. R. Cronje</name><affiliation><nlm:aff id="fsn3210-aff-0004"></nlm:aff></affiliation></author><author><name sortKey="Landahl, Sandra" sort="Landahl, Sandra" uniqKey="Landahl S" first="Sandra" last="Landahl">Sandra Landahl</name><affiliation><nlm:aff id="fsn3210-aff-0005"></nlm:aff></affiliation></author><author><name sortKey="Ortiz, Jose Ordaz" sort="Ortiz, Jose Ordaz" uniqKey="Ortiz J" first="Jose Ordaz" last="Ortiz">Jose Ordaz Ortiz</name><affiliation><nlm:aff id="fsn3210-aff-0005"></nlm:aff></affiliation></author><author><name sortKey="Terry, Leon A" sort="Terry, Leon A" uniqKey="Terry L" first="Leon A." last="Terry">Leon A. Terry</name><affiliation><nlm:aff id="fsn3210-aff-0005"></nlm:aff></affiliation></author></analytic><series><title level="j">Food Science & Nutrition</title><idno type="eISSN">2048-7177</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Abstract</title><p>Conventional methods for extracting and quantifying phenolic compounds in citrus rinds are time consuming. Rapid methods for extracting and quantifying phenolic compounds were developed by comparing three extraction solvent combinations (80:20 v/v ethanol:H<sub>2</sub>O; 70:29.5:0.5 v/v/v methanol:H<sub>2</sub>O:<styled-content style="fixed-case">HC</styled-content>l; and 50:50 v/v dimethyl sulfoxide (<styled-content style="fixed-case">DMSO</styled-content>):methanol) for effectiveness. Freeze‐dried, rind powder was extracted in an ultrasonic water bath at 35°C for 10, 20, and 30 min. Phenolic compound quantification was done with a high‐performance liquid chromatography (<styled-content style="fixed-case">HPLC</styled-content>) equipped with diode array detector. Extracting with methanol:H<sub>2</sub>O:<styled-content style="fixed-case">HC</styled-content>l for 30 min resulted in the optimum yield of targeted phenolic acids. Seven phenolic acids and three flavanone glycosides (<styled-content style="fixed-case">FG</styled-content>s) were quantified. The dominant phenolic compound was hesperidin, with concentrations ranging from 7500 to 32,000 <italic>μ</italic>g/g <styled-content style="fixed-case">DW</styled-content>. The highest yield of <styled-content style="fixed-case">FG</styled-content>s was observed in samples extracted, using <styled-content style="fixed-case">DMSO</styled-content>:methanol for 10 min. Compared to other extraction methods, methanol:H<sub>2</sub>O:<styled-content style="fixed-case">HC</styled-content>l was efficient in optimum extraction of phenolic acids. The limit of detection and quantification for all analytes were small, ranging from 1.35 to 5.02 and 4.51 to 16.72 <italic>μ</italic>g/g <styled-content style="fixed-case">DW</styled-content>, respectively, demonstrating <styled-content style="fixed-case">HPLC</styled-content> quantification method sensitivity. The extraction and quantification methods developed in this study are faster and more efficient. Where speed and effectiveness are required, these methods are recommended.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Terry, L A" uniqKey="Terry L">L. A. Terry</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Santos Uelga, C" uniqKey="Santos Uelga C">C. Santos‐Buelga</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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