Search for Allergens from the Pollen Proteome of Sunflower (Helianthus annuus L.): A Major Sensitizer for Respiratory Allergy Patients
Identifieur interne : 001D27 ( Ncbi/Curation ); précédent : 001D26; suivant : 001D28Search for Allergens from the Pollen Proteome of Sunflower (Helianthus annuus L.): A Major Sensitizer for Respiratory Allergy Patients
Auteurs : Nandini Ghosh [Inde] ; Gaurab Sircar [Inde] ; Bodhisattwa Saha [Inde] ; Naren Pandey [Inde] ; Swati Gupta Bhattacharya [Inde]Source :
- PLoS ONE [ 1932-6203 ] ; 2015.
Abstract
Respiratory allergy triggered by pollen allergens is increasing at an alarming rate worldwide. Sunflower pollen is thought to be an important source of inhalant allergens. Present study aims to identify the prevalence of sunflower pollinosis among the Indian allergic population and characterizes the pollen allergens using immuno-proteomic tools.
Clinico-immunological tests were performed to understand the prevalence of sensitivity towards sunflower pollen among the atopic population. Sera from selected sunflower positive patients were used as probe to detect the IgE-reactive proteins from the one and two dimensional electrophoretic separated proteome of sunflower pollen. The antigenic nature of the sugar moiety of the glycoallergens was studied by meta-periodate modification of IgE-immunoblot. Finally, these allergens were identified by mass-spectrometry.
Prevalence of sunflower pollen sensitization was observed among 21% of the pollen allergic population and associated with elevated level of specific IgE and histamine in the sera of these patients. Immunoscreening of sunflower pollen proteome with patient sera detected seven IgE-reactive proteins with varying molecular weight and pI. Hierarchical clustering of 2D-immunoblot data highlighted three allergens characterized by a more frequent immuno-reactivity and increased levels of IgE antibodies in the sera of susceptible patients. These allergens were considered as the major allergens of sunflower pollen and were found to have their glycan moiety critical for inducing IgE response. Homology driven search of MS/MS data of these IgE-reactive proteins identified seven previously unreported allergens from sunflower pollen. Three major allergenic proteins were identified as two pectate lyases and a cysteine protease.
Novelty of the present report is the identification of a panel of seven sunflower pollen allergens for the first time at immuno-biochemical and proteomic level, which substantiated the clinical evidence of sunflower allergy. Further purification and recombinant expression of these allergens will improve component-resolved diagnosis and therapy of pollen allergy.
Url:
DOI: 10.1371/journal.pone.0138992
PubMed: 26418046
PubMed Central: 4587886
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Search for Allergens from the Pollen Proteome of Sunflower (<italic>Helianthus annuus</italic> L.): A Major Sensitizer for Respiratory Allergy Patients</title><author><name sortKey="Ghosh, Nandini" sort="Ghosh, Nandini" uniqKey="Ghosh N" first="Nandini" last="Ghosh">Nandini Ghosh</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Division of Plant Biology, Bose Institute, Kolkata, West Bengal, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biology, Bose Institute, Kolkata, West Bengal</wicri:regionArea><wicri:noRegion>West Bengal</wicri:noRegion></affiliation></author><author><name sortKey="Sircar, Gaurab" sort="Sircar, Gaurab" uniqKey="Sircar G" first="Gaurab" last="Sircar">Gaurab Sircar</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Division of Plant Biology, Bose Institute, Kolkata, West Bengal, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biology, Bose Institute, Kolkata, West Bengal</wicri:regionArea><wicri:noRegion>West Bengal</wicri:noRegion></affiliation></author><author><name sortKey="Saha, Bodhisattwa" sort="Saha, Bodhisattwa" uniqKey="Saha B" first="Bodhisattwa" last="Saha">Bodhisattwa Saha</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Division of Plant Biology, Bose Institute, Kolkata, West Bengal, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biology, Bose Institute, Kolkata, West Bengal</wicri:regionArea><wicri:noRegion>West Bengal</wicri:noRegion></affiliation></author><author><name sortKey="Pandey, Naren" sort="Pandey, Naren" uniqKey="Pandey N" first="Naren" last="Pandey">Naren Pandey</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Allergy and Asthma, Belle Vue Clinic, Kolkata, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Allergy and Asthma, Belle Vue Clinic, Kolkata</wicri:regionArea><wicri:noRegion>Kolkata</wicri:noRegion></affiliation></author><author><name sortKey="Gupta Bhattacharya, Swati" sort="Gupta Bhattacharya, Swati" uniqKey="Gupta Bhattacharya S" first="Swati" last="Gupta Bhattacharya">Swati Gupta Bhattacharya</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Division of Plant Biology, Bose Institute, Kolkata, West Bengal, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biology, Bose Institute, Kolkata, West Bengal</wicri:regionArea><wicri:noRegion>West Bengal</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26418046</idno><idno type="pmc">4587886</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4587886</idno><idno type="RBID">PMC:4587886</idno><idno type="doi">10.1371/journal.pone.0138992</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000193</idno><idno type="wicri:Area/Pmc/Curation">000193</idno><idno type="wicri:Area/Pmc/Checkpoint">000358</idno><idno type="wicri:Area/Ncbi/Merge">001D27</idno><idno type="wicri:Area/Ncbi/Curation">001D27</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Search for Allergens from the Pollen Proteome of Sunflower (<italic>Helianthus annuus</italic> L.): A Major Sensitizer for Respiratory Allergy Patients</title><author><name sortKey="Ghosh, Nandini" sort="Ghosh, Nandini" uniqKey="Ghosh N" first="Nandini" last="Ghosh">Nandini Ghosh</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Division of Plant Biology, Bose Institute, Kolkata, West Bengal, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biology, Bose Institute, Kolkata, West Bengal</wicri:regionArea><wicri:noRegion>West Bengal</wicri:noRegion></affiliation></author><author><name sortKey="Sircar, Gaurab" sort="Sircar, Gaurab" uniqKey="Sircar G" first="Gaurab" last="Sircar">Gaurab Sircar</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Division of Plant Biology, Bose Institute, Kolkata, West Bengal, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biology, Bose Institute, Kolkata, West Bengal</wicri:regionArea><wicri:noRegion>West Bengal</wicri:noRegion></affiliation></author><author><name sortKey="Saha, Bodhisattwa" sort="Saha, Bodhisattwa" uniqKey="Saha B" first="Bodhisattwa" last="Saha">Bodhisattwa Saha</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Division of Plant Biology, Bose Institute, Kolkata, West Bengal, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biology, Bose Institute, Kolkata, West Bengal</wicri:regionArea><wicri:noRegion>West Bengal</wicri:noRegion></affiliation></author><author><name sortKey="Pandey, Naren" sort="Pandey, Naren" uniqKey="Pandey N" first="Naren" last="Pandey">Naren Pandey</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Allergy and Asthma, Belle Vue Clinic, Kolkata, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Allergy and Asthma, Belle Vue Clinic, Kolkata</wicri:regionArea><wicri:noRegion>Kolkata</wicri:noRegion></affiliation></author><author><name sortKey="Gupta Bhattacharya, Swati" sort="Gupta Bhattacharya, Swati" uniqKey="Gupta Bhattacharya S" first="Swati" last="Gupta Bhattacharya">Swati Gupta Bhattacharya</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Division of Plant Biology, Bose Institute, Kolkata, West Bengal, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biology, Bose Institute, Kolkata, West Bengal</wicri:regionArea><wicri:noRegion>West Bengal</wicri:noRegion></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="sec001"><title>Background</title><p>Respiratory allergy triggered by pollen allergens is increasing at an alarming rate worldwide. Sunflower pollen is thought to be an important source of inhalant allergens. Present study aims to identify the prevalence of sunflower pollinosis among the Indian allergic population and characterizes the pollen allergens using immuno-proteomic tools.</p></sec><sec id="sec002"><title>Methodology</title><p>Clinico-immunological tests were performed to understand the prevalence of sensitivity towards sunflower pollen among the atopic population. Sera from selected sunflower positive patients were used as probe to detect the IgE-reactive proteins from the one and two dimensional electrophoretic separated proteome of sunflower pollen. The antigenic nature of the sugar moiety of the glycoallergens was studied by meta-periodate modification of IgE-immunoblot. Finally, these allergens were identified by mass-spectrometry.</p></sec><sec id="sec003"><title>Results</title><p>Prevalence of sunflower pollen sensitization was observed among 21% of the pollen allergic population and associated with elevated level of specific IgE and histamine in the sera of these patients. Immunoscreening of sunflower pollen proteome with patient sera detected seven IgE-reactive proteins with varying molecular weight and pI. Hierarchical clustering of 2D-immunoblot data highlighted three allergens characterized by a more frequent immuno-reactivity and increased levels of IgE antibodies in the sera of susceptible patients. These allergens were considered as the major allergens of sunflower pollen and were found to have their glycan moiety critical for inducing IgE response. Homology driven search of MS/MS data of these IgE-reactive proteins identified seven previously unreported allergens from sunflower pollen. Three major allergenic proteins were identified as two pectate lyases and a cysteine protease.</p></sec><sec id="sec004"><title>Conclusion</title><p>Novelty of the present report is the identification of a panel of seven sunflower pollen allergens for the first time at immuno-biochemical and proteomic level, which substantiated the clinical evidence of sunflower allergy. 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uniqKey="Rementeria A">A Rementeria</name></author><author><name sortKey="Pont N, J" uniqKey="Pont N J">J Pontón</name></author><author><name sortKey="Hernando, Fl" uniqKey="Hernando F">FL Hernando</name></author></analytic></biblStruct></listBibl></div1></back></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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