Alterations in the activity and structure of pectin methylesterase treated by high pressure carbon dioxide.
Identifieur interne : 000954 ( Ncbi/Curation ); précédent : 000953; suivant : 000955Alterations in the activity and structure of pectin methylesterase treated by high pressure carbon dioxide.
Auteurs : Linyan Zhou [République populaire de Chine] ; Jihong Wu ; Xiaosong Hu ; Xian Zhi ; Xiaojun LiaoSource :
- Journal of agricultural and food chemistry [ 1520-5118 ] ; 2009.
English descriptors
- KwdEn :
- MESH :
- chemical , antagonists & inhibitors : Carboxylic Ester Hydrolases, Plant Proteins.
- chemical , chemistry : Carboxylic Ester Hydrolases, Plant Proteins.
- chemical , pharmacology : Carbon Dioxide.
- enzymology : Citrus sinensis.
- Enzyme Stability, Pressure.
Abstract
The influence of high pressure carbon dioxide (HPCD) on the activity and structure of pectin methylesterase (PME) from orange was investigated. The pressures were 8-30 MPa, temperature 55 degrees C and time 10 min. HPCD caused significant inactivation on PME, the lowest residual activity was about 9.3% at 30 MPa. The SDS-PAGE electrophoretic behavior of HPCD-treated PME was not altered, while changes in the secondary and tertiary structures were found. The beta-structure fraction in the secondary structure decreased and the fluorescence intensity increased as HPCD pressures were elevated. After 7-day storage at 4 degrees C, no alteration of its activity and no reversion of its beta-structure fraction were observed, while its fluorescence intensity further decreased.
DOI: 10.1021/jf803501q
PubMed: 19256556
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pubmed:19256556Le document en format XML
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100083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wu, Jihong" sort="Wu, Jihong" uniqKey="Wu J" first="Jihong" last="Wu">Jihong Wu</name></author><author><name sortKey="Hu, Xiaosong" sort="Hu, Xiaosong" uniqKey="Hu X" first="Xiaosong" last="Hu">Xiaosong Hu</name></author><author><name sortKey="Zhi, Xian" sort="Zhi, Xian" uniqKey="Zhi X" first="Xian" last="Zhi">Xian Zhi</name></author><author><name sortKey="Liao, Xiaojun" sort="Liao, Xiaojun" uniqKey="Liao X" first="Xiaojun" last="Liao">Xiaojun Liao</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19256556</idno><idno type="pmid">19256556</idno><idno type="doi">10.1021/jf803501q</idno><idno type="wicri:Area/PubMed/Corpus">000972</idno><idno type="wicri:Area/PubMed/Curation">000972</idno><idno type="wicri:Area/PubMed/Checkpoint">000972</idno><idno type="wicri:Area/Ncbi/Merge">000954</idno><idno type="wicri:Area/Ncbi/Curation">000954</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Alterations in the activity and structure of pectin methylesterase treated by high pressure carbon dioxide.</title><author><name sortKey="Zhou, Linyan" sort="Zhou, Linyan" uniqKey="Zhou L" first="Linyan" last="Zhou">Linyan Zhou</name><affiliation wicri:level="1"><nlm:affiliation>College of Food Science and Nutritional Engineering, China Agricultural University, Key Laboratory of Fruit & Vegetable Processing, Ministry of Agriculture, Engineering Research Center for Fruit & Vegetable Processing, Ministry of Education, Beijing 100083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Food Science and Nutritional Engineering, China Agricultural University, Key Laboratory of Fruit & Vegetable Processing, Ministry of Agriculture, Engineering Research Center for Fruit & Vegetable Processing, Ministry of Education, Beijing 100083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wu, Jihong" sort="Wu, Jihong" uniqKey="Wu J" first="Jihong" last="Wu">Jihong Wu</name></author><author><name sortKey="Hu, Xiaosong" sort="Hu, Xiaosong" uniqKey="Hu X" first="Xiaosong" last="Hu">Xiaosong Hu</name></author><author><name sortKey="Zhi, Xian" sort="Zhi, Xian" uniqKey="Zhi X" first="Xian" last="Zhi">Xian Zhi</name></author><author><name sortKey="Liao, Xiaojun" sort="Liao, Xiaojun" uniqKey="Liao X" first="Xiaojun" last="Liao">Xiaojun Liao</name></author></analytic><series><title level="j">Journal of agricultural and food chemistry</title><idno type="eISSN">1520-5118</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carbon Dioxide (pharmacology)</term><term>Carboxylic Ester Hydrolases (antagonists & inhibitors)</term><term>Carboxylic Ester Hydrolases (chemistry)</term><term>Citrus sinensis (enzymology)</term><term>Enzyme Stability</term><term>Plant Proteins (antagonists & inhibitors)</term><term>Plant Proteins (chemistry)</term><term>Pressure</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Carboxylic Ester Hydrolases</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Carboxylic Ester Hydrolases</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Carbon Dioxide</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Enzyme Stability</term><term>Pressure</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The influence of high pressure carbon dioxide (HPCD) on the activity and structure of pectin methylesterase (PME) from orange was investigated. The pressures were 8-30 MPa, temperature 55 degrees C and time 10 min. HPCD caused significant inactivation on PME, the lowest residual activity was about 9.3% at 30 MPa. The SDS-PAGE electrophoretic behavior of HPCD-treated PME was not altered, while changes in the secondary and tertiary structures were found. The beta-structure fraction in the secondary structure decreased and the fluorescence intensity increased as HPCD pressures were elevated. After 7-day storage at 4 degrees C, no alteration of its activity and no reversion of its beta-structure fraction were observed, while its fluorescence intensity further decreased.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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