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Differentiation of Strains of Xylella fastidiosa by a Variable Number of Tandem Repeat Analysis

Identifieur interne : 000033 ( Ncbi/Checkpoint ); précédent : 000032; suivant : 000034

Differentiation of Strains of Xylella fastidiosa by a Variable Number of Tandem Repeat Analysis

Auteurs : Helvécio Della Coletta-Filho ; Marco Aurélio Takita ; Alessandra Alves De Souza ; Carlos Ivan Aguilar-Vildoso ; Marcos Antonio Machado

Source :

Applied and Environmental Microbiology [ 0099-2240 ] ; 2001.

RBID : PMC:93134

Abstract

Short sequence repeats (SSRs) with a potential variable number of tandem repeat (VNTR) loci were identified in the genome of the citrus pathogen Xylella fastidiosa and used for typing studies. Although mono- and dinucleotide repeats were absent, we found several intermediate-length 7-, 8-, and 9-nucleotide repeats, which we examined for allelic polymorphisms using PCR. Five genuine VNTR loci were highly polymorphic within a set of 27 X. fastidiosa strains from different hosts. The highest average Nei's measure of genetic diversity (H) estimated for VNTR loci was 0.51, compared to 0.17 derived from randomly amplified polymorphic DNA (RAPD) analysis. For citrus X. fastidiosa strains, some specific VNTR loci had a H value of 0.83, while the maximum value given by specific RAPD loci was 0.12. Our approach using VNTR markers provides a high-resolution tool for epidemiological, genetic, and ecological analysis of citrus-specific X. fastidiosa strains.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93134

DOI: 10.1128/AEM.67.9.4091-4095.2001
PubMed: 11526010
PubMed Central: 93134

Affiliations:

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PMC:93134

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Differentiation of Strains of <italic>Xylella fastidiosa</italic>
 by a Variable Number of Tandem Repeat Analysis</title>
<author><name sortKey="Coletta Filho, Helvecio Della" sort="Coletta Filho, Helvecio Della" uniqKey="Coletta Filho H" first="Helvécio Della" last="Coletta-Filho">Helvécio Della Coletta-Filho</name>
</author>
<author><name sortKey="Takita, Marco Aurelio" sort="Takita, Marco Aurelio" uniqKey="Takita M" first="Marco Aurélio" last="Takita">Marco Aurélio Takita</name>
</author>
<author><name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name>
</author>
<author><name sortKey="Aguilar Vildoso, Carlos Ivan" sort="Aguilar Vildoso, Carlos Ivan" uniqKey="Aguilar Vildoso C" first="Carlos Ivan" last="Aguilar-Vildoso">Carlos Ivan Aguilar-Vildoso</name>
</author>
<author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name>
</author>
</titleStmt>
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<idno type="pmid">11526010</idno>
<idno type="pmc">93134</idno>
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<idno type="RBID">PMC:93134</idno>
<idno type="doi">10.1128/AEM.67.9.4091-4095.2001</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Differentiation of Strains of <italic>Xylella fastidiosa</italic>
 by a Variable Number of Tandem Repeat Analysis</title>
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<author><name sortKey="Takita, Marco Aurelio" sort="Takita, Marco Aurelio" uniqKey="Takita M" first="Marco Aurélio" last="Takita">Marco Aurélio Takita</name>
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<author><name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name>
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<author><name sortKey="Aguilar Vildoso, Carlos Ivan" sort="Aguilar Vildoso, Carlos Ivan" uniqKey="Aguilar Vildoso C" first="Carlos Ivan" last="Aguilar-Vildoso">Carlos Ivan Aguilar-Vildoso</name>
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<author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name>
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<series><title level="j">Applied and Environmental Microbiology</title>
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<front><div type="abstract" xml:lang="en"><p>Short sequence repeats (SSRs) with a potential variable number of tandem repeat (VNTR) loci were identified in the genome of the citrus pathogen <italic>Xylella fastidiosa</italic>
 and used for typing studies. Although mono- and dinucleotide repeats were absent, we found several intermediate-length 7-, 8-, and 9-nucleotide repeats, which we examined for allelic polymorphisms using PCR. Five genuine VNTR loci were highly polymorphic within a set of 27 <italic>X. fastidiosa</italic>
 strains from different hosts. The highest average Nei's measure of genetic diversity (<italic>H</italic>
) estimated for VNTR loci was 0.51, compared to 0.17 derived from randomly amplified polymorphic DNA (RAPD) analysis. For citrus <italic>X. fastidiosa</italic>
 strains, some specific VNTR loci had a <italic>H</italic>
 value of 0.83, while the maximum value given by specific RAPD loci was 0.12. Our approach using VNTR markers provides a high-resolution tool for epidemiological, genetic, and ecological analysis of citrus-specific <italic>X. fastidiosa</italic>
 strains.</p>
</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Aguilar Vildoso, Carlos Ivan" sort="Aguilar Vildoso, Carlos Ivan" uniqKey="Aguilar Vildoso C" first="Carlos Ivan" last="Aguilar-Vildoso">Carlos Ivan Aguilar-Vildoso</name>
<name sortKey="Coletta Filho, Helvecio Della" sort="Coletta Filho, Helvecio Della" uniqKey="Coletta Filho H" first="Helvécio Della" last="Coletta-Filho">Helvécio Della Coletta-Filho</name>
<name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name>
<name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name>
<name sortKey="Takita, Marco Aurelio" sort="Takita, Marco Aurelio" uniqKey="Takita M" first="Marco Aurélio" last="Takita">Marco Aurélio Takita</name>
</noCountry>
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</record>

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Differentiation of Strains of Xylella fastidiosa by a Variable Number of Tandem Repeat Analysis

Differentiation of Strains of Xylella fastidiosa by a Variable Number of Tandem Repeat Analysis

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki