Direct somatic embryogenesis and plant regeneration from protoplasts of Bupleurum scorzonerifolium Willd
Identifieur interne : 003D12 ( Main/Merge ); précédent : 003D11; suivant : 003D13Direct somatic embryogenesis and plant regeneration from protoplasts of Bupleurum scorzonerifolium Willd
Auteurs : Xia Guang-Min [République populaire de Chine] ; Li Zhongyi [République populaire de Chine] ; Guo Guang-Qin [République populaire de Chine] ; Chen Hui-Min [République populaire de Chine]Source :
- Plant Cell Reports [ 0721-7714 ] ; 1992-04-01.
Abstract
Abstract: Protolasts of Bupleurum scorzonerifolium were prepared from stem node-derived embryogenic calli with an enzyeme mixture, in which snailase was a necessary component. Follolwing cell wall regeneration protoplasts divided and directly formed somatic embryos which developed into plantlets. The conditions favorable to direct embryo formation were investigated, and the nature of the callus used for protoplast preparation was found to be a critical factor. The osmotic concentration and the composition of the culture medium including the phytohormone combinations were also important.
Url:
DOI: 10.1007/BF00232170
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