OrangerV1, Main, Merge, bibRecord, 002F92

Somatic embryogenesis in Citrus spp.: carbohydrate stimulation and histodifferentiation

Identifieur interne : 002F92 ( Main/Merge ); précédent : 002F91; suivant : 002F93

Somatic embryogenesis in Citrus spp.: carbohydrate stimulation and histodifferentiation

Auteurs : Marcio L. Tomaz [Brésil] ; Beatriz M. Januzzi Mendes [Brésil] ; Francisco De Assis A. Mourao Filho [Brésil] ; Clarice G. B. Demetrio [Brésil] ; Naratip Jansakul [Royaume-Uni] ; Adriana P. Martinelli Rodriguez [Brésil]

Source :

In vitro cellular & developmental biology. Plant [ 1054-5476 ] ; 2001.

RBID : Pascal:01-0376965

Descripteurs français

Pascal (Inist)
- Embryon somatique, Développement embryonnaire, Cytodifférenciation, Culture tissu, Multiplication végétative in vitro, Comparaison intraspécifique, Cultivar, Composition chimique, Milieu culture, Citrus sinensis, Citrus reticulata, Saccharose, Galactose, Glucose, Lactose, Maltose, Nutrition, Histologie, Stade développement, Régénération, Glycérol, Citrus limonia.
Wicri :
- topic : Saccharose, Glucose, Lactose, Nutrition, Histologie.

English descriptors

KwdEn :
- Cell differentiation, Chemical composition, Citrus reticulata, Citrus sinensis, Cultivar, Culture medium, Developmental stage, Embryonic development, Galactose, Glucose, Glycerol, Histology, Intraspecific comparison, Lactose, Maltose, Micropropagation, Nutrition, Regeneration, Somatic embryo, Sucrose, Tissue culture.

Abstract

Somatic embryogenesis from nucellus-derived callus cultures of five cultivars, including three (Caipira, Seleta Vermelha, and Valencia) of sweet oranges (C. sinensis L. Osbeck), Rangpur lime (C. limonia L. Osbeck), and Cleopatra mandarin (C. reticulata Blanco) (lines I and II), were studied. Callus lines maintained on MT medium supplemented with 50 g l^-1 sucrose were transferred to MT medium supplemented with different carbohydrate sources: galactose, glucose, lactose, maltose, or sucrose at 18, 37, 75, 110, or 150 mM, or glycerol at 6, 12, 24, 36, or 50 mM. Globular embryos were observed after approximately 4 wk, in several treatments. Cultures of Valencia and Caipira sweet oranges and Cleopatra mandarin (line I) showed high numbers of embryos on medium containing galactose, lactose, and maltose. Histological studies showed somatic embryos in all developmental stages with a normal histodifferentiation pattern. The other two cultivars (Rangpur lime and Cleopatra mandarin, line II) formed very few embryos, which did not develop further following the globular stage. Some of the abnormalities observed were lack or dedifferentiation of protoderm and absence of apical meristems and procambial strands. Embryos that followed the normal sequence of development were easily converted into plants. Non-embryogenic cultures continued as proliferating callus cultures, eventually forming a few embryos which did not convert into plants. Statistical analyses of the callus response to carbohydrate treatments was done using an overdispersion Poisson model.

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000883
to stream PascalFrancis, to step Curation: 000121
to stream PascalFrancis, to step Checkpoint: 000836

Links to Exploration step

Pascal:01-0376965

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Somatic embryogenesis in Citrus spp.: carbohydrate stimulation and histodifferentiation</title>
<author><name sortKey="Tomaz, Marcio L" sort="Tomaz, Marcio L" uniqKey="Tomaz M" first="Marcio L." last="Tomaz">Marcio L. Tomaz</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Escola Superior de Agricultura Luiz de Queiroz'</s1>
<s2>13418-900 Piracicaba/SP</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Brésil</country>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Januzzi Mendes, Beatriz M" sort="Januzzi Mendes, Beatriz M" uniqKey="Januzzi Mendes B" first="Beatriz M." last="Januzzi Mendes">Beatriz M. Januzzi Mendes</name>
<affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Laboratório de Biotecnologia Vegetal, Centro de Energia Nuclear na Agricultura/USP, Av. Centenário 303</s1>
<s2>13416-970 Piracicaba/SP</s2>
<s3>BRA</s3>
<sZ>2 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Brésil</country>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mourao Filho, Francisco De Assis A" sort="Mourao Filho, Francisco De Assis A" uniqKey="Mourao Filho F" first="Francisco De Assis A." last="Mourao Filho">Francisco De Assis A. Mourao Filho</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Escola Superior de Agricultura Luiz de Queiroz'</s1>
<s2>13418-900 Piracicaba/SP</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Brésil</country>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Demetrio, Clarice G B" sort="Demetrio, Clarice G B" uniqKey="Demetrio C" first="Clarice G. B." last="Demetrio">Clarice G. B. Demetrio</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Escola Superior de Agricultura Luiz de Queiroz'</s1>
<s2>13418-900 Piracicaba/SP</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Brésil</country>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Jansakul, Naratip" sort="Jansakul, Naratip" uniqKey="Jansakul N" first="Naratip" last="Jansakul">Naratip Jansakul</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>University of Exeter</s1>
<s2>Exeter</s2>
<s3>GBR</s3>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Royaume-Uni</country>
<wicri:noRegion>University of Exeter</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Martinelli Rodriguez, Adriana P" sort="Martinelli Rodriguez, Adriana P" uniqKey="Martinelli Rodriguez A" first="Adriana P." last="Martinelli Rodriguez">Adriana P. Martinelli Rodriguez</name>
<affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Laboratório de Biotecnologia Vegetal, Centro de Energia Nuclear na Agricultura/USP, Av. Centenário 303</s1>
<s2>13416-970 Piracicaba/SP</s2>
<s3>BRA</s3>
<sZ>2 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Brésil</country>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">01-0376965</idno>
<date when="2001">2001</date>
<idno type="stanalyst">PASCAL 01-0376965 INIST</idno>
<idno type="RBID">Pascal:01-0376965</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000883</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000121</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000836</idno>
<idno type="wicri:doubleKey">1054-5476:2001:Tomaz M:somatic:embryogenesis:in</idno>
<idno type="wicri:Area/Main/Merge">002F92</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Somatic embryogenesis in Citrus spp.: carbohydrate stimulation and histodifferentiation</title>
<author><name sortKey="Tomaz, Marcio L" sort="Tomaz, Marcio L" uniqKey="Tomaz M" first="Marcio L." last="Tomaz">Marcio L. Tomaz</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Escola Superior de Agricultura Luiz de Queiroz'</s1>
<s2>13418-900 Piracicaba/SP</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Brésil</country>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Januzzi Mendes, Beatriz M" sort="Januzzi Mendes, Beatriz M" uniqKey="Januzzi Mendes B" first="Beatriz M." last="Januzzi Mendes">Beatriz M. Januzzi Mendes</name>
<affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Laboratório de Biotecnologia Vegetal, Centro de Energia Nuclear na Agricultura/USP, Av. Centenário 303</s1>
<s2>13416-970 Piracicaba/SP</s2>
<s3>BRA</s3>
<sZ>2 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Brésil</country>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mourao Filho, Francisco De Assis A" sort="Mourao Filho, Francisco De Assis A" uniqKey="Mourao Filho F" first="Francisco De Assis A." last="Mourao Filho">Francisco De Assis A. Mourao Filho</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Escola Superior de Agricultura Luiz de Queiroz'</s1>
<s2>13418-900 Piracicaba/SP</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Brésil</country>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Demetrio, Clarice G B" sort="Demetrio, Clarice G B" uniqKey="Demetrio C" first="Clarice G. B." last="Demetrio">Clarice G. B. Demetrio</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Escola Superior de Agricultura Luiz de Queiroz'</s1>
<s2>13418-900 Piracicaba/SP</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Brésil</country>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Jansakul, Naratip" sort="Jansakul, Naratip" uniqKey="Jansakul N" first="Naratip" last="Jansakul">Naratip Jansakul</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>University of Exeter</s1>
<s2>Exeter</s2>
<s3>GBR</s3>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Royaume-Uni</country>
<wicri:noRegion>University of Exeter</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Martinelli Rodriguez, Adriana P" sort="Martinelli Rodriguez, Adriana P" uniqKey="Martinelli Rodriguez A" first="Adriana P." last="Martinelli Rodriguez">Adriana P. Martinelli Rodriguez</name>
<affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Laboratório de Biotecnologia Vegetal, Centro de Energia Nuclear na Agricultura/USP, Av. Centenário 303</s1>
<s2>13416-970 Piracicaba/SP</s2>
<s3>BRA</s3>
<sZ>2 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Brésil</country>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">In vitro cellular & developmental biology. Plant</title>
<title level="j" type="abbreviated">In vitro cell. dev. biol., Plant</title>
<idno type="ISSN">1054-5476</idno>
<imprint><date when="2001">2001</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">In vitro cellular & developmental biology. Plant</title>
<title level="j" type="abbreviated">In vitro cell. dev. biol., Plant</title>
<idno type="ISSN">1054-5476</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell differentiation</term>
<term>Chemical composition</term>
<term>Citrus reticulata</term>
<term>Citrus sinensis</term>
<term>Cultivar</term>
<term>Culture medium</term>
<term>Developmental stage</term>
<term>Embryonic development</term>
<term>Galactose</term>
<term>Glucose</term>
<term>Glycerol</term>
<term>Histology</term>
<term>Intraspecific comparison</term>
<term>Lactose</term>
<term>Maltose</term>
<term>Micropropagation</term>
<term>Nutrition</term>
<term>Regeneration</term>
<term>Somatic embryo</term>
<term>Sucrose</term>
<term>Tissue culture</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Embryon somatique</term>
<term>Développement embryonnaire</term>
<term>Cytodifférenciation</term>
<term>Culture tissu</term>
<term>Multiplication végétative in vitro</term>
<term>Comparaison intraspécifique</term>
<term>Cultivar</term>
<term>Composition chimique</term>
<term>Milieu culture</term>
<term>Citrus sinensis</term>
<term>Citrus reticulata</term>
<term>Saccharose</term>
<term>Galactose</term>
<term>Glucose</term>
<term>Lactose</term>
<term>Maltose</term>
<term>Nutrition</term>
<term>Histologie</term>
<term>Stade développement</term>
<term>Régénération</term>
<term>Glycérol</term>
<term>Citrus limonia</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Saccharose</term>
<term>Glucose</term>
<term>Lactose</term>
<term>Nutrition</term>
<term>Histologie</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Somatic embryogenesis from nucellus-derived callus cultures of five cultivars, including three (Caipira, Seleta Vermelha, and Valencia) of sweet oranges (C. sinensis L. Osbeck), Rangpur lime (C. limonia L. Osbeck), and Cleopatra mandarin (C. reticulata Blanco) (lines I and II), were studied. Callus lines maintained on MT medium supplemented with 50 g l<sup>-1</sup>
 sucrose were transferred to MT medium supplemented with different carbohydrate sources: galactose, glucose, lactose, maltose, or sucrose at 18, 37, 75, 110, or 150 mM, or glycerol at 6, 12, 24, 36, or 50 mM. Globular embryos were observed after approximately 4 wk, in several treatments. Cultures of Valencia and Caipira sweet oranges and Cleopatra mandarin (line I) showed high numbers of embryos on medium containing galactose, lactose, and maltose. Histological studies showed somatic embryos in all developmental stages with a normal histodifferentiation pattern. The other two cultivars (Rangpur lime and Cleopatra mandarin, line II) formed very few embryos, which did not develop further following the globular stage. Some of the abnormalities observed were lack or dedifferentiation of protoderm and absence of apical meristems and procambial strands. Embryos that followed the normal sequence of development were easily converted into plants. Non-embryogenic cultures continued as proliferating callus cultures, eventually forming a few embryos which did not convert into plants. Statistical analyses of the callus response to carbohydrate treatments was done using an overdispersion Poisson model.</div>
</front>
</TEI>
<affiliations><list><country><li>Brésil</li>
<li>Royaume-Uni</li>
</country>
<region><li>État de São Paulo</li>
</region>
</list>
<tree><country name="Brésil"><region name="État de São Paulo"><name sortKey="Tomaz, Marcio L" sort="Tomaz, Marcio L" uniqKey="Tomaz M" first="Marcio L." last="Tomaz">Marcio L. Tomaz</name>
</region>
<name sortKey="Demetrio, Clarice G B" sort="Demetrio, Clarice G B" uniqKey="Demetrio C" first="Clarice G. B." last="Demetrio">Clarice G. B. Demetrio</name>
<name sortKey="Januzzi Mendes, Beatriz M" sort="Januzzi Mendes, Beatriz M" uniqKey="Januzzi Mendes B" first="Beatriz M." last="Januzzi Mendes">Beatriz M. Januzzi Mendes</name>
<name sortKey="Martinelli Rodriguez, Adriana P" sort="Martinelli Rodriguez, Adriana P" uniqKey="Martinelli Rodriguez A" first="Adriana P." last="Martinelli Rodriguez">Adriana P. Martinelli Rodriguez</name>
<name sortKey="Mourao Filho, Francisco De Assis A" sort="Mourao Filho, Francisco De Assis A" uniqKey="Mourao Filho F" first="Francisco De Assis A." last="Mourao Filho">Francisco De Assis A. Mourao Filho</name>
</country>
<country name="Royaume-Uni"><noRegion><name sortKey="Jansakul, Naratip" sort="Jansakul, Naratip" uniqKey="Jansakul N" first="Naratip" last="Jansakul">Naratip Jansakul</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Merge

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002F92 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 002F92 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Bois
   |area=    OrangerV1
   |flux=    Main
   |étape=   Merge
   |type=    RBID
   |clé=     Pascal:01-0376965
   |texte=   Somatic embryogenesis in Citrus spp.: carbohydrate stimulation and histodifferentiation
}}

This area was generated with Dilib version V0.6.25.
Data generation: Sat Dec 3 17:11:04 2016. Site generation: Wed Mar 6 18:18:32 2024

	Serveur d'exploration sur l'oranger
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur l'oranger

Somatic embryogenesis in Citrus spp.: carbohydrate stimulation and histodifferentiation

Somatic embryogenesis in Citrus spp.: carbohydrate stimulation and histodifferentiation

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri