Enantioseparation of imazalil residue in orange by capillary electrophoresis with 2-hydroxypropyl-beta-cyclodextrin as a chiral selector.
Identifieur interne : 002C08 ( Main/Merge ); précédent : 002C07; suivant : 002C09Enantioseparation of imazalil residue in orange by capillary electrophoresis with 2-hydroxypropyl-beta-cyclodextrin as a chiral selector.
Auteurs : Shuji Kodama [Japon] ; Atsushi Yamamoto ; Takashi Ohura ; Akinobu Matsunaga ; Toshio KanbeSource :
- Journal of agricultural and food chemistry [ 0021-8561 ] ; 2003.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Imidazoles.
- chemical , isolation & purification : Imidazoles.
- chemical : Cyclodextrins, Indicators and Reagents, beta-Cyclodextrins.
- chemistry : Citrus sinensis, Fruit.
- Electrophoresis, Capillary, Stereoisomerism.
Abstract
Chiral resolution of imazalil, a fungicide, was performed by capillary electrophoresis (CE) using 2-hydroxypropyl-beta-cyclodextrin as a chiral selector. Factors affecting the chiral resolution and migration time of imazalil were studied. The optimum running conditions were found to be 5 mM ammonium dihydrogenphosphate-50 mM phosphate buffer (pH 3.0) containing 4 mM 2-hydroxypropyl-beta-cyclodextrin with an effective voltage of +25 kV at 20 degrees C using direct detection at 200 nm. Under these conditions, the resolution (Rs) of racemic imazalil was approximately 6. The extraction of imazalil from orange samples was done with acetonitrile under basic conditions. The extract was purified with a solid-phase extraction cartridge (Sep-Pak plus PS-2) and was analyzed by the above CE method. Eight orange samples were analyzed, and imazalil was detected in seven samples. In four of these seven oranges, the level of (-)-imazalil was the same as that of (+)-imazalil, but in the other three oranges, the level of (-)-imazalil was found to be lower than that of (+)-imazalil, suggesting that (-)-imazalil was degraded more quickly than (+)-imazalil in oranges.
DOI: 10.1021/jf030282x
PubMed: 14518933
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pubmed:14518933Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Enantioseparation of imazalil residue in orange by capillary electrophoresis with 2-hydroxypropyl-beta-cyclodextrin as a chiral selector.</title><author><name sortKey="Kodama, Shuji" sort="Kodama, Shuji" uniqKey="Kodama S" first="Shuji" last="Kodama">Shuji Kodama</name><affiliation wicri:level="1"><nlm:affiliation>Toyama Institute of Health, 17-1 Nakataikoyama, Kosugi-machi, Toyama 939-0363, Japan. shuji.kodama@pref.toyama.lg.jp</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Toyama Institute of Health, 17-1 Nakataikoyama, Kosugi-machi, Toyama 939-0363</wicri:regionArea><wicri:noRegion>Toyama 939-0363</wicri:noRegion></affiliation></author><author><name sortKey="Yamamoto, Atsushi" sort="Yamamoto, Atsushi" uniqKey="Yamamoto A" first="Atsushi" last="Yamamoto">Atsushi Yamamoto</name></author><author><name sortKey="Ohura, Takashi" sort="Ohura, Takashi" uniqKey="Ohura T" first="Takashi" last="Ohura">Takashi Ohura</name></author><author><name sortKey="Matsunaga, Akinobu" sort="Matsunaga, Akinobu" uniqKey="Matsunaga A" first="Akinobu" last="Matsunaga">Akinobu Matsunaga</name></author><author><name sortKey="Kanbe, Toshio" sort="Kanbe, Toshio" uniqKey="Kanbe T" first="Toshio" last="Kanbe">Toshio Kanbe</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:14518933</idno><idno type="pmid">14518933</idno><idno type="doi">10.1021/jf030282x</idno><idno type="wicri:Area/PubMed/Corpus">000E24</idno><idno type="wicri:Area/PubMed/Curation">000E24</idno><idno type="wicri:Area/PubMed/Checkpoint">000E24</idno><idno type="wicri:Area/Ncbi/Merge">000220</idno><idno type="wicri:Area/Ncbi/Curation">000220</idno><idno type="wicri:Area/Ncbi/Checkpoint">000220</idno><idno type="wicri:doubleKey">0021-8561:2003:Kodama S:enantioseparation:of:imazalil</idno><idno type="wicri:Area/Main/Merge">002C08</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Enantioseparation of imazalil residue in orange by capillary electrophoresis with 2-hydroxypropyl-beta-cyclodextrin as a chiral selector.</title><author><name sortKey="Kodama, Shuji" sort="Kodama, Shuji" uniqKey="Kodama S" first="Shuji" last="Kodama">Shuji Kodama</name><affiliation wicri:level="1"><nlm:affiliation>Toyama Institute of Health, 17-1 Nakataikoyama, Kosugi-machi, Toyama 939-0363, Japan. shuji.kodama@pref.toyama.lg.jp</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Toyama Institute of Health, 17-1 Nakataikoyama, Kosugi-machi, Toyama 939-0363</wicri:regionArea><wicri:noRegion>Toyama 939-0363</wicri:noRegion></affiliation></author><author><name sortKey="Yamamoto, Atsushi" sort="Yamamoto, Atsushi" uniqKey="Yamamoto A" first="Atsushi" last="Yamamoto">Atsushi Yamamoto</name></author><author><name sortKey="Ohura, Takashi" sort="Ohura, Takashi" uniqKey="Ohura T" first="Takashi" last="Ohura">Takashi Ohura</name></author><author><name sortKey="Matsunaga, Akinobu" sort="Matsunaga, Akinobu" uniqKey="Matsunaga A" first="Akinobu" last="Matsunaga">Akinobu Matsunaga</name></author><author><name sortKey="Kanbe, Toshio" sort="Kanbe, Toshio" uniqKey="Kanbe T" first="Toshio" last="Kanbe">Toshio Kanbe</name></author></analytic><series><title level="j">Journal of agricultural and food chemistry</title><idno type="ISSN">0021-8561</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus sinensis (chemistry)</term><term>Cyclodextrins</term><term>Electrophoresis, Capillary</term><term>Fruit (chemistry)</term><term>Imidazoles (chemistry)</term><term>Imidazoles (isolation & purification)</term><term>Indicators and Reagents</term><term>Stereoisomerism</term><term>beta-Cyclodextrins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Imidazoles</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Imidazoles</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Cyclodextrins</term><term>Indicators and Reagents</term><term>beta-Cyclodextrins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term><term>Fruit</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Electrophoresis, Capillary</term><term>Stereoisomerism</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Chiral resolution of imazalil, a fungicide, was performed by capillary electrophoresis (CE) using 2-hydroxypropyl-beta-cyclodextrin as a chiral selector. Factors affecting the chiral resolution and migration time of imazalil were studied. The optimum running conditions were found to be 5 mM ammonium dihydrogenphosphate-50 mM phosphate buffer (pH 3.0) containing 4 mM 2-hydroxypropyl-beta-cyclodextrin with an effective voltage of +25 kV at 20 degrees C using direct detection at 200 nm. Under these conditions, the resolution (Rs) of racemic imazalil was approximately 6. The extraction of imazalil from orange samples was done with acetonitrile under basic conditions. The extract was purified with a solid-phase extraction cartridge (Sep-Pak plus PS-2) and was analyzed by the above CE method. Eight orange samples were analyzed, and imazalil was detected in seven samples. In four of these seven oranges, the level of (-)-imazalil was the same as that of (+)-imazalil, but in the other three oranges, the level of (-)-imazalil was found to be lower than that of (+)-imazalil, suggesting that (-)-imazalil was degraded more quickly than (+)-imazalil in oranges.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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