Factors influencing Agrobacterium-mediated embryogenic callus transformation of Valencia sweet orange (Citrus sinensis) containing the pTA29-barnase gene.
Identifieur interne : 002C06 ( Main/Merge ); précédent : 002C05; suivant : 002C07Factors influencing Agrobacterium-mediated embryogenic callus transformation of Valencia sweet orange (Citrus sinensis) containing the pTA29-barnase gene.
Auteurs : D D Li [République populaire de Chine] ; W. Shi ; X X DengSource :
- Tree physiology [ 0829-318X ] ; 2003.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Acetophenones.
- genetics : Agrobacterium tumefaciens, Citrus sinensis, Genes, Bacterial, Genetic Vectors, Plant Tumors, Plants, Genetically Modified.
- Blotting, Southern, Polymerase Chain Reaction.
Abstract
Valencia sweet orange (Citrus sinensis (L.) Osbeck) calluses were used as explants to develop a new transformation system for citrus mediated by Agrobacterium tumefaciens. Factors affecting Agrobacterium-mediated transformation efficiency included mode of pre-cultivation, temperature of cocultivation and presence of acetosyringone (AS). The highest transformation efficiency was obtained with a 4-day pre-cultivation period in liquid medium. Transformation efficiency was higher when cocultivation was performed for 3 days at 19 degrees C than at 23 or 28 degrees C. Almost no resistant callus was obtained if the cocultivation medium lacked AS. The transformation procedure yielded transgenic Valencia plants containing the pTA29-barnase gene, as verified by PCR amplification and confirmed by Southern blotting. Because male sterility is a common factor leading to seedlessness in citrus cultivars with parthenocarpic characteristics, production of seedless citrus genotypes by Agrobacterium-mediated genetic transformation is a promising alternative to conventional breeding methods.
PubMed: 14597430
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pubmed:14597430Le document en format XML
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China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><wicri:noRegion>Wuhan 430070</wicri:noRegion></affiliation></author><author><name sortKey="Shi, W" sort="Shi, W" uniqKey="Shi W" first="W" last="Shi">W. Shi</name></author><author><name sortKey="Deng, X X" sort="Deng, X X" uniqKey="Deng X" first="X X" last="Deng">X X Deng</name></author></analytic><series><title level="j">Tree physiology</title><idno type="ISSN">0829-318X</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetophenones (metabolism)</term><term>Agrobacterium tumefaciens (genetics)</term><term>Blotting, Southern</term><term>Citrus sinensis (genetics)</term><term>Genes, Bacterial (genetics)</term><term>Genetic Vectors (genetics)</term><term>Plant Tumors (genetics)</term><term>Plants, Genetically Modified (genetics)</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Acetophenones</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Citrus sinensis</term><term>Genes, Bacterial</term><term>Genetic Vectors</term><term>Plant Tumors</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Southern</term><term>Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Valencia sweet orange (Citrus sinensis (L.) Osbeck) calluses were used as explants to develop a new transformation system for citrus mediated by Agrobacterium tumefaciens. Factors affecting Agrobacterium-mediated transformation efficiency included mode of pre-cultivation, temperature of cocultivation and presence of acetosyringone (AS). The highest transformation efficiency was obtained with a 4-day pre-cultivation period in liquid medium. Transformation efficiency was higher when cocultivation was performed for 3 days at 19 degrees C than at 23 or 28 degrees C. Almost no resistant callus was obtained if the cocultivation medium lacked AS. The transformation procedure yielded transgenic Valencia plants containing the pTA29-barnase gene, as verified by PCR amplification and confirmed by Southern blotting. Because male sterility is a common factor leading to seedlessness in citrus cultivars with parthenocarpic characteristics, production of seedless citrus genotypes by Agrobacterium-mediated genetic transformation is a promising alternative to conventional breeding methods.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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