Citrus abscission and Arabidopsis plant decline in response to 5-chloro-3-methyl-4-nitro-1H-pyrazole are mediated by lipid signalling
Identifieur interne : 002985 ( Main/Merge ); précédent : 002984; suivant : 002986Citrus abscission and Arabidopsis plant decline in response to 5-chloro-3-methyl-4-nitro-1H-pyrazole are mediated by lipid signalling
Auteurs : Fernando Alferez [États-Unis] ; Shila Singh [États-Unis] ; Ann L. Umbach [États-Unis] ; Brandon Hockema [États-Unis] ; Jacqueline K. Burns [États-Unis]Source :
- Plant, cell and environment : (Print) [ 0140-7791 ] ; 2005.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Fruit.
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Abstract
The compound 5-chloro-3-niethyl-4-nitro-1H-pyrazole (CMNP) is a pyrazole-derivative that induces abscission selectively in mature citrus (Citrus sinensis) fruit when applied to the canopy and has herbicidal activity on plants when applied to roots. Despite the favourable efficacy of this compound, the mode of action remains unknown. To gain information about the mode of action of CMNP, the effect of application to mature citrus fruit and Arabidopsis thaliana roots was explored. Peel contact was essential for mature fruit abscission in citrus, whereas root drenching was essential for symptom development and plant decline in Arabidopsis. CMNP was identified as an uncoupler in isolated soybean (Glycine max) mitochondria and pea (Pisum sativum) chloroplasts and an inhibitor of alcohol dehydrogenase in citrus peel, but not an inhibitor of proto-porphyrinogen IX oxidase. CMNP treatment reduced ATP content in citrus peel and Arabidopsis leaves. Phospholipase A2 (PLA2) and lipoxygenase (LOX) activities, and lipid hydroperoxide (LPO) levels increased in flavedo of citrus fruit peel and leaves of Arabidopsis plants treated with CMNP. An inhibitor of PLA2 activity, aristolochic acid (AT), reduced CMNP-induced increases in PLA2 and LOX activities and LPO levels in citrus flavedo and Arabidopsis leaves and greatly reduced abscission in citrus and delayed symptoms of plant decline in Arabidopsis. However, AT treatment failed to halt the reduction in ATP content. Reduction in ATP content preceded the increase in PLA2 and LOX activities, LPO content and the biological response. The results indicate a link between lipid signalling, abscission in citrus and herbicidal damage in Arabidopsis.
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Umbach</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Duke University, DCMB Group/Biology Department</s1><s2>Durham, North Carolina, 27708-1000</s2><s3>USA</s3><sZ>3 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">District de Columbia</region></placeName></affiliation></author><author><name sortKey="Hockema, Brandon" sort="Hockema, Brandon" uniqKey="Hockema B" first="Brandon" last="Hockema">Brandon Hockema</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>University of Florida, IFAS, Horticultural Sciences Department, Citrus Research and Education Center, 700 Experiment Station Road</s1><s2>Lake Alfred, Florida 33850-2299</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Lake Alfred, Florida 33850-2299</wicri:noRegion></affiliation></author><author><name sortKey="Burns, Jacqueline K" sort="Burns, Jacqueline K" uniqKey="Burns J" first="Jacqueline K." last="Burns">Jacqueline K. Burns</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>University of Florida, IFAS, Horticultural Sciences Department, Citrus Research and Education Center, 700 Experiment Station Road</s1><s2>Lake Alfred, Florida 33850-2299</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Lake Alfred, Florida 33850-2299</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Plant, cell and environment : (Print)</title><title level="j" type="abbreviated">Plant cell environ. : (Print)</title><idno type="ISSN">0140-7791</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Plant, cell and environment : (Print)</title><title level="j" type="abbreviated">Plant cell environ. : (Print)</title><idno type="ISSN">0140-7791</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>ATP</term><term>Abscission</term><term>Alcohol dehydrogenase</term><term>Arabidopsis thaliana</term><term>Chloroplast</term><term>Citrus sinensis</term><term>Development</term><term>Fruit</term><term>Glycine max</term><term>Lipids</term><term>Lipoxygenase</term><term>Mitochondria</term><term>Oxidase</term><term>Pisum sativum</term><term>Plant leaf</term><term>Pyrazole derivatives</term><term>Root</term><term>Senescence</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Abscission</term><term>Lipide</term><term>Pyrazole dérivé</term><term>Fruit</term><term>Racine</term><term>Développement</term><term>Mitochondrie</term><term>Chloroplaste</term><term>Alcohol dehydrogenase</term><term>Citrus sinensis</term><term>Arabidopsis thaliana</term><term>Glycine max</term><term>Pisum sativum</term><term>Oxidase</term><term>ATP</term><term>Feuille végétal</term><term>Lipoxygenase</term><term>Sénescence</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Fruit</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The compound 5-chloro-3-niethyl-4-nitro-1H-pyrazole (CMNP) is a pyrazole-derivative that induces abscission selectively in mature citrus (Citrus sinensis) fruit when applied to the canopy and has herbicidal activity on plants when applied to roots. Despite the favourable efficacy of this compound, the mode of action remains unknown. To gain information about the mode of action of CMNP, the effect of application to mature citrus fruit and Arabidopsis thaliana roots was explored. Peel contact was essential for mature fruit abscission in citrus, whereas root drenching was essential for symptom development and plant decline in Arabidopsis. CMNP was identified as an uncoupler in isolated soybean (Glycine max) mitochondria and pea (Pisum sativum) chloroplasts and an inhibitor of alcohol dehydrogenase in citrus peel, but not an inhibitor of proto-porphyrinogen IX oxidase. CMNP treatment reduced ATP content in citrus peel and Arabidopsis leaves. Phospholipase A<sub>2</sub> (PLA<sub>2</sub>) and lipoxygenase (LOX) activities, and lipid hydroperoxide (LPO) levels increased in flavedo of citrus fruit peel and leaves of Arabidopsis plants treated with CMNP. An inhibitor of PLA<sub>2</sub> activity, aristolochic acid (AT), reduced CMNP-induced increases in PLA<sub>2</sub> and LOX activities and LPO levels in citrus flavedo and Arabidopsis leaves and greatly reduced abscission in citrus and delayed symptoms of plant decline in Arabidopsis. However, AT treatment failed to halt the reduction in ATP content. Reduction in ATP content preceded the increase in PLA<sub>2</sub> and LOX activities, LPO content and the biological response. The results indicate a link between lipid signalling, abscission in citrus and herbicidal damage in Arabidopsis.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>District de Columbia</li></region></list><tree><country name="États-Unis"><noRegion><name sortKey="Alferez, Fernando" sort="Alferez, Fernando" uniqKey="Alferez F" first="Fernando" last="Alferez">Fernando Alferez</name></noRegion><name sortKey="Burns, Jacqueline K" sort="Burns, Jacqueline K" uniqKey="Burns J" first="Jacqueline K." last="Burns">Jacqueline K. Burns</name><name sortKey="Hockema, Brandon" sort="Hockema, Brandon" uniqKey="Hockema B" first="Brandon" last="Hockema">Brandon Hockema</name><name sortKey="Singh, Shila" sort="Singh, Shila" uniqKey="Singh S" first="Shila" last="Singh">Shila Singh</name><name sortKey="Umbach, Ann L" sort="Umbach, Ann L" uniqKey="Umbach A" first="Ann L." last="Umbach">Ann L. 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