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Protoplast co-transformation and regeneration of transgenic 'Hamlin' sweet orange plants containing a cDNA Xa21 xanthomonas resistance gene and GFP

Identifieur interne : 002523 ( Main/Merge ); précédent : 002522; suivant : 002524

Protoplast co-transformation and regeneration of transgenic 'Hamlin' sweet orange plants containing a cDNA Xa21 xanthomonas resistance gene and GFP

Auteurs : A. A. Omar [États-Unis] ; J. W. Grosser [États-Unis]

Source :

Acta horticulturae [ 0567-7572 ] ; 2007.

RBID : Pascal:07-0538144

Descripteurs français

Pascal (Inist)
- Plante transgénique, Cultivar, Bactériose, Résistance microorganisme, Gène indicateur, Gène structure, Multigène, Protoplaste, Régénération, Transformation génétique, Citrus sinensis, Oryza sativa, Xanthomonas axonopodis, DNA complémentaire, Plasmide, Protéine fluorescente verte, Culture cellulaire, Système combiné, cv Hamlin, Gène gfp, Gène Xa21, Xanthomonas oryzae, Chancre plante.
Wicri :
- topic : Plante transgénique.

English descriptors

KwdEn :
- Bacteriosis, Cell culture, Citrus sinensis, Combined system, Complementary DNA, Cultivar, Genetic transformation, Green fluorescent protein, Microorganism resistance, Multigene family, Plant cankers, Protoplast, Regeneration, Reporter gene, Structural gene, Transgenic plant, Xanthomonas axonopodis.

Abstract

[...] Plasmid DNA (pARS108) encoding the non-destructive selectable marker EGFP (Enhanced Green Fluorescent Protein) gene, and the plasmid cDNA of the Xa21 gene (pXa21-mtaq) were co-transformed into 'Hamlin' orange protoplasts using polyethylene glycol. More than 150 transgenic embryoids were recovered. Over a thousand transgenic plantlets GFP positive were regenerated from 150 independent transformation events. PCR analysis revealed the presence of the cDNA of the Xa21 and the GFP genes in some of the transgenic plantlets. The recovery of transgenic plants was expedited by in vitro grafting.[...]

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Pascal:07-0538144

Le document en format XML

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<s2>Lake Alfred, FL</s2>
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<placeName><region type="state">Floride</region>
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<term>Complementary DNA</term>
<term>Cultivar</term>
<term>Genetic transformation</term>
<term>Green fluorescent protein</term>
<term>Microorganism resistance</term>
<term>Multigene family</term>
<term>Plant cankers</term>
<term>Protoplast</term>
<term>Regeneration</term>
<term>Reporter gene</term>
<term>Structural gene</term>
<term>Transgenic plant</term>
<term>Xanthomonas axonopodis</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Plante transgénique</term>
<term>Cultivar</term>
<term>Bactériose</term>
<term>Résistance microorganisme</term>
<term>Gène indicateur</term>
<term>Gène structure</term>
<term>Multigène</term>
<term>Protoplaste</term>
<term>Régénération</term>
<term>Transformation génétique</term>
<term>Citrus sinensis</term>
<term>Oryza sativa</term>
<term>Xanthomonas axonopodis</term>
<term>DNA complémentaire</term>
<term>Plasmide</term>
<term>Protéine fluorescente verte</term>
<term>Culture cellulaire</term>
<term>Système combiné</term>
<term>cv Hamlin</term>
<term>Gène gfp</term>
<term>Gène Xa21</term>
<term>Xanthomonas oryzae</term>
<term>Chancre plante</term>
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<front><div type="abstract" xml:lang="en">[...] Plasmid DNA (pARS108) encoding the non-destructive selectable marker EGFP (Enhanced Green Fluorescent Protein) gene, and the plasmid cDNA of the Xa21 gene (pXa21-mtaq) were co-transformed into 'Hamlin' orange protoplasts using polyethylene glycol. More than 150 transgenic embryoids were recovered. Over a thousand transgenic plantlets GFP positive were regenerated from 150 independent transformation events. PCR analysis revealed the presence of the cDNA of the Xa21 and the GFP genes in some of the transgenic plantlets. The recovery of transgenic plants was expedited by in vitro grafting.[...]</div>
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<name sortKey="Grosser, J W" sort="Grosser, J W" uniqKey="Grosser J" first="J. W." last="Grosser">J. W. Grosser</name>
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Protoplast co-transformation and regeneration of transgenic 'Hamlin' sweet orange plants containing a cDNA Xa21 xanthomonas resistance gene and GFP

Protoplast co-transformation and regeneration of transgenic 'Hamlin' sweet orange plants containing a cDNA Xa21 xanthomonas resistance gene and GFP

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri