mRNA from selected genes is useful for specific detection and quantification of viable Xanthomonas citri subsp. citri
Identifieur interne : 001936 ( Istex/Corpus ); précédent : 001935; suivant : 001937mRNA from selected genes is useful for specific detection and quantification of viable Xanthomonas citri subsp. citri
Auteurs : M. Golmohammadi ; P. Llop ; G. Scuderi ; I. Gell ; J. H. Graham ; J. CuberoSource :
- Plant Pathology [ 0032-0862 ] ; 2012-06.
English descriptors
Abstract
The purpose of this study was to assess the stability of mRNA and rRNA for evaluation of viability for Xanthomonas citri subsp. citri (Xcc). Total RNA from Xcc suspensions subjected to different stress treatments (high temperature or chemical treatment with sodium orthophenylphenate at different concentrations) was extracted at different time periods post‐treatment (0, 3, 24 and 48 h) and analysed by quantitative real‐time reverse transcription PCR (Q‐RT‐PCR). Primers were designed from selected fragments of rRNA and mRNA from genes involved in bacterial fitness, virulence or general metabolic mechanisms (gumD, rpfB, avrBs2 and gyrB). After stress treatment, only a 445‐bp fragment from the gumD mRNA was detected in live Xcc cells specifically, whereas other RNA fragments, as well as DNA targets, were detected in both viable and nonviable cells. Statistical analyses demonstrated that the amount of some transcripts from genes involved in xanthan synthesis, pathogenicity factor regulation and DNA processing was significantly reduced after lethal treatments. The amplification of the 445‐bp product from gumD mRNA was demonstrated to be useful for the detection of viable Xcc; the product was detected specifically from viable bacteria on leaf and citrus fruit surfaces and in citrus canker lesions. Instability of long RNA fragments can be used as a practical tool for the study of survival of citrus canker bacteria or for diagnostic purposes when the presence of viable bacteria needs to be confirmed.
Url:
DOI: 10.1111/j.1365-3059.2011.02526.x
Links to Exploration step
ISTEX:3360682130BACCA3166C8B91442BE69FF5F117E1Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">mRNA from selected genes is useful for specific detection and quantification of viable Xanthomonas citri subsp. citri</title><author><name sortKey="Golmohammadi, M" sort="Golmohammadi, M" uniqKey="Golmohammadi M" first="M." last="Golmohammadi">M. Golmohammadi</name><affiliation><mods:affiliation>Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia, Spain</mods:affiliation></affiliation></author><author><name sortKey="Llop, P" sort="Llop, P" uniqKey="Llop P" first="P." last="Llop">P. Llop</name><affiliation><mods:affiliation>Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia, Spain</mods:affiliation></affiliation></author><author><name sortKey="Scuderi, G" sort="Scuderi, G" uniqKey="Scuderi G" first="G." last="Scuderi">G. Scuderi</name><affiliation><mods:affiliation>Department of Phytosanitary Sciences and Technologies (DISTEF), Plant Pathology section, University of Catania, 95123 Catania, Italy</mods:affiliation></affiliation></author><author><name sortKey="Gell, I" sort="Gell, I" uniqKey="Gell I" first="I." last="Gell">I. Gell</name><affiliation><mods:affiliation>Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de La Coruña km 7·5, Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Graham, J H" sort="Graham, J H" uniqKey="Graham J" first="J. H." last="Graham">J. H. Graham</name><affiliation><mods:affiliation>University of Florida, Citrus Research and Education Center (CREC) 700 Experiment Station Road, Lake Alfred, FL 33850‐2299, USA</mods:affiliation></affiliation></author><author><name sortKey="Cubero, J" sort="Cubero, J" uniqKey="Cubero J" first="J." last="Cubero">J. Cubero</name><affiliation><mods:affiliation>Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de La Coruña km 7·5, Madrid, Spain</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:3360682130BACCA3166C8B91442BE69FF5F117E1</idno><date when="2012" year="2012">2012</date><idno type="doi">10.1111/j.1365-3059.2011.02526.x</idno><idno type="url">https://api.istex.fr/document/3360682130BACCA3166C8B91442BE69FF5F117E1/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001936</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">mRNA from selected genes is useful for specific detection and quantification of viable Xanthomonas citri subsp. citri</title><author><name sortKey="Golmohammadi, M" sort="Golmohammadi, M" uniqKey="Golmohammadi M" first="M." last="Golmohammadi">M. Golmohammadi</name><affiliation><mods:affiliation>Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia, Spain</mods:affiliation></affiliation></author><author><name sortKey="Llop, P" sort="Llop, P" uniqKey="Llop P" first="P." last="Llop">P. Llop</name><affiliation><mods:affiliation>Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia, Spain</mods:affiliation></affiliation></author><author><name sortKey="Scuderi, G" sort="Scuderi, G" uniqKey="Scuderi G" first="G." last="Scuderi">G. Scuderi</name><affiliation><mods:affiliation>Department of Phytosanitary Sciences and Technologies (DISTEF), Plant Pathology section, University of Catania, 95123 Catania, Italy</mods:affiliation></affiliation></author><author><name sortKey="Gell, I" sort="Gell, I" uniqKey="Gell I" first="I." last="Gell">I. Gell</name><affiliation><mods:affiliation>Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de La Coruña km 7·5, Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Graham, J H" sort="Graham, J H" uniqKey="Graham J" first="J. H." last="Graham">J. H. Graham</name><affiliation><mods:affiliation>University of Florida, Citrus Research and Education Center (CREC) 700 Experiment Station Road, Lake Alfred, FL 33850‐2299, USA</mods:affiliation></affiliation></author><author><name sortKey="Cubero, J" sort="Cubero, J" uniqKey="Cubero J" first="J." last="Cubero">J. Cubero</name><affiliation><mods:affiliation>Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de La Coruña km 7·5, Madrid, Spain</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant Pathology</title><idno type="ISSN">0032-0862</idno><idno type="eISSN">1365-3059</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2012-06">2012-06</date><biblScope unit="volume">61</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="479">479</biblScope><biblScope unit="page" to="488">488</biblScope></imprint><idno type="ISSN">0032-0862</idno></series><idno type="istex">3360682130BACCA3166C8B91442BE69FF5F117E1</idno><idno type="DOI">10.1111/j.1365-3059.2011.02526.x</idno><idno type="ArticleID">PPA2526</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0032-0862</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus</term><term>bacterial survival</term><term>citrus bacterial canker</term><term>real‐time PCR</term><term>viable bacteria</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The purpose of this study was to assess the stability of mRNA and rRNA for evaluation of viability for Xanthomonas citri subsp. citri (Xcc). Total RNA from Xcc suspensions subjected to different stress treatments (high temperature or chemical treatment with sodium orthophenylphenate at different concentrations) was extracted at different time periods post‐treatment (0, 3, 24 and 48 h) and analysed by quantitative real‐time reverse transcription PCR (Q‐RT‐PCR). Primers were designed from selected fragments of rRNA and mRNA from genes involved in bacterial fitness, virulence or general metabolic mechanisms (gumD, rpfB, avrBs2 and gyrB). After stress treatment, only a 445‐bp fragment from the gumD mRNA was detected in live Xcc cells specifically, whereas other RNA fragments, as well as DNA targets, were detected in both viable and nonviable cells. Statistical analyses demonstrated that the amount of some transcripts from genes involved in xanthan synthesis, pathogenicity factor regulation and DNA processing was significantly reduced after lethal treatments. The amplification of the 445‐bp product from gumD mRNA was demonstrated to be useful for the detection of viable Xcc; the product was detected specifically from viable bacteria on leaf and citrus fruit surfaces and in citrus canker lesions. Instability of long RNA fragments can be used as a practical tool for the study of survival of citrus canker bacteria or for diagnostic purposes when the presence of viable bacteria needs to be confirmed.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>M. Golmohammadi</name><affiliations><json:string>Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia, Spain</json:string></affiliations></json:item><json:item><name>P. Llop</name><affiliations><json:string>Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia, Spain</json:string></affiliations></json:item><json:item><name>G. Scuderi</name><affiliations><json:string>Department of Phytosanitary Sciences and Technologies (DISTEF), Plant Pathology section, University of Catania, 95123 Catania, Italy</json:string></affiliations></json:item><json:item><name>I. Gell</name><affiliations><json:string>Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de La Coruña km 7·5, Madrid, Spain</json:string></affiliations></json:item><json:item><name>J. H. Graham</name><affiliations><json:string>University of Florida, Citrus Research and Education Center (CREC) 700 Experiment Station Road, Lake Alfred, FL 33850‐2299, USA</json:string></affiliations></json:item><json:item><name>J. Cubero</name><affiliations><json:string>Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de La Coruña km 7·5, Madrid, Spain</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>bacterial survival</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>citrus bacterial canker</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Citrus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>real‐time PCR</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>viable bacteria</value></json:item></subject><articleId><json:string>PPA2526</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>The purpose of this study was to assess the stability of mRNA and rRNA for evaluation of viability for Xanthomonas citri subsp. citri (Xcc). Total RNA from Xcc suspensions subjected to different stress treatments (high temperature or chemical treatment with sodium orthophenylphenate at different concentrations) was extracted at different time periods post‐treatment (0, 3, 24 and 48 h) and analysed by quantitative real‐time reverse transcription PCR (Q‐RT‐PCR). Primers were designed from selected fragments of rRNA and mRNA from genes involved in bacterial fitness, virulence or general metabolic mechanisms (gumD, rpfB, avrBs2 and gyrB). After stress treatment, only a 445‐bp fragment from the gumD mRNA was detected in live Xcc cells specifically, whereas other RNA fragments, as well as DNA targets, were detected in both viable and nonviable cells. Statistical analyses demonstrated that the amount of some transcripts from genes involved in xanthan synthesis, pathogenicity factor regulation and DNA processing was significantly reduced after lethal treatments. The amplification of the 445‐bp product from gumD mRNA was demonstrated to be useful for the detection of viable Xcc; the product was detected specifically from viable bacteria on leaf and citrus fruit surfaces and in citrus canker lesions. Instability of long RNA fragments can be used as a practical tool for the study of survival of citrus canker bacteria or for diagnostic purposes when the presence of viable bacteria needs to be confirmed.</abstract><qualityIndicators><score>7.736</score><pdfVersion>1.3</pdfVersion><pdfPageSize>595.276 x 782.362 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>5</keywordCount><abstractCharCount>1516</abstractCharCount><pdfWordCount>6889</pdfWordCount><pdfCharCount>42577</pdfCharCount><pdfPageCount>10</pdfPageCount><abstractWordCount>228</abstractWordCount></qualityIndicators><title>mRNA from selected genes is useful for specific detection and quantification of viable Xanthomonas citri subsp. citri</title><genre><json:string>article</json:string></genre><host><volume>61</volume><publisherId><json:string>PPA</json:string></publisherId><pages><total>10</total><last>488</last><first>479</first></pages><issn><json:string>0032-0862</json:string></issn><issue>3</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1365-3059</json:string></eissn><title>Plant Pathology</title><doi><json:string>10.1111/(ISSN)1365-3059</json:string></doi></host><publicationDate>2012</publicationDate><copyrightDate>2012</copyrightDate><doi><json:string>10.1111/j.1365-3059.2011.02526.x</json:string></doi><id>3360682130BACCA3166C8B91442BE69FF5F117E1</id><score>0.13609812</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/3360682130BACCA3166C8B91442BE69FF5F117E1/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/3360682130BACCA3166C8B91442BE69FF5F117E1/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/3360682130BACCA3166C8B91442BE69FF5F117E1/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">mRNA from selected genes is useful for specific detection and quantification of viable Xanthomonas citri subsp. citri</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><availability><p>© 2011 The Authors. Plant Pathology © 2011 BSPP</p></availability><date>2012</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">mRNA from selected genes is useful for specific detection and quantification of viable Xanthomonas citri subsp. citri</title><author xml:id="author-1"><persName><forename type="first">M.</forename><surname>Golmohammadi</surname></persName><note type="biography">Note: First and second authors contributed equally to the paper.</note><note type="biography">Current address: Department of Plant Protection, Iran Citrus Research Institute, Ramsar, Iran.</note><affiliation>Note: First and second authors contributed equally to the paper.</affiliation><affiliation>Current address: Department of Plant Protection, Iran Citrus Research Institute, Ramsar, Iran.</affiliation><affiliation>Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia, Spain</affiliation></author><author xml:id="author-2"><persName><forename type="first">P.</forename><surname>Llop</surname></persName><note type="correspondence"><p>Correspondence: E‐mail:</p></note><affiliation>Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia, Spain</affiliation></author><author xml:id="author-3"><persName><forename type="first">G.</forename><surname>Scuderi</surname></persName><affiliation>Department of Phytosanitary Sciences and Technologies (DISTEF), Plant Pathology section, University of Catania, 95123 Catania, Italy</affiliation></author><author xml:id="author-4"><persName><forename type="first">I.</forename><surname>Gell</surname></persName><affiliation>Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de La Coruña km 7·5, Madrid, Spain</affiliation></author><author xml:id="author-5"><persName><forename type="first">J. H.</forename><surname>Graham</surname></persName><affiliation>University of Florida, Citrus Research and Education Center (CREC) 700 Experiment Station Road, Lake Alfred, FL 33850‐2299, USA</affiliation></author><author xml:id="author-6"><persName><forename type="first">J.</forename><surname>Cubero</surname></persName><note type="correspondence"><p>Correspondence: E‐mail:</p></note><affiliation>Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de La Coruña km 7·5, Madrid, Spain</affiliation></author></analytic><monogr><title level="j">Plant Pathology</title><idno type="pISSN">0032-0862</idno><idno type="eISSN">1365-3059</idno><idno type="DOI">10.1111/(ISSN)1365-3059</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2012-06"></date><biblScope unit="volume">61</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="479">479</biblScope><biblScope unit="page" to="488">488</biblScope></imprint></monogr><idno type="istex">3360682130BACCA3166C8B91442BE69FF5F117E1</idno><idno type="DOI">10.1111/j.1365-3059.2011.02526.x</idno><idno type="ArticleID">PPA2526</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2012</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The purpose of this study was to assess the stability of mRNA and rRNA for evaluation of viability for Xanthomonas citri subsp. citri (Xcc). Total RNA from Xcc suspensions subjected to different stress treatments (high temperature or chemical treatment with sodium orthophenylphenate at different concentrations) was extracted at different time periods post‐treatment (0, 3, 24 and 48 h) and analysed by quantitative real‐time reverse transcription PCR (Q‐RT‐PCR). Primers were designed from selected fragments of rRNA and mRNA from genes involved in bacterial fitness, virulence or general metabolic mechanisms (gumD, rpfB, avrBs2 and gyrB). After stress treatment, only a 445‐bp fragment from the gumD mRNA was detected in live Xcc cells specifically, whereas other RNA fragments, as well as DNA targets, were detected in both viable and nonviable cells. Statistical analyses demonstrated that the amount of some transcripts from genes involved in xanthan synthesis, pathogenicity factor regulation and DNA processing was significantly reduced after lethal treatments. The amplification of the 445‐bp product from gumD mRNA was demonstrated to be useful for the detection of viable Xcc; the product was detected specifically from viable bacteria on leaf and citrus fruit surfaces and in citrus canker lesions. Instability of long RNA fragments can be used as a practical tool for the study of survival of citrus canker bacteria or for diagnostic purposes when the presence of viable bacteria needs to be confirmed.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>bacterial survival</term></item><item><term>citrus bacterial canker</term></item><item><term>Citrus</term></item><item><term>real‐time PCR</term></item><item><term>viable bacteria</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2012-06">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/3360682130BACCA3166C8B91442BE69FF5F117E1/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1365-3059</doi><issn type="print">0032-0862</issn><issn type="electronic">1365-3059</issn><idGroup><id type="product" value="PPA"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="PLANT PATHOLOGY">Plant Pathology</title></titleGroup></publicationMeta><publicationMeta level="part" position="06103"><doi origin="wiley">10.1111/ppa.2012.61.issue-3</doi><numberingGroup><numbering type="journalVolume" number="61">61</numbering><numbering type="journalIssue" number="3">3</numbering></numberingGroup><coverDate startDate="2012-06">June 2012</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="6" status="forIssue"><doi origin="wiley">10.1111/j.1365-3059.2011.02526.x</doi><idGroup><id type="unit" value="PPA2526"></id></idGroup><countGroup><count type="pageTotal" number="10"></count></countGroup><titleGroup><title type="tocHeading1">Original Articles</title></titleGroup><copyright>© 2011 The Authors. Plant Pathology © 2011 BSPP</copyright><eventGroup><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:3.1.5 mode:FullText" date="2012-05-09"></event><event type="publishedOnlineEarlyUnpaginated" date="2011-08-15"></event><event type="firstOnline" date="2011-08-15"></event><event type="publishedOnlineFinalForm" date="2012-05-09"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-02-07"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.3.7 mode:FullText" date="2015-03-24"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="479">479</numbering><numbering type="pageLast" number="488">488</numbering></numberingGroup><correspondenceTo> E‐mail: <email>cubero@inia.es</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:PPA.PPA2526.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Published online 14 August 2011</unparsedEditorialHistory><countGroup><count type="figureTotal" number="2"></count><count type="tableTotal" number="3"></count></countGroup><titleGroup><title type="main">mRNA from selected genes is useful for specific detection and quantification of viable <i>Xanthomonas citri</i> subsp. <i>citri</i></title><title type="shortAuthors"><i>M. Golmohammadi </i>et al.</title><title type="short"><i>Detection of viable cells of</i> Xanthomonas citri<i>subsp</i>. citri</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1" noteRef="#fn1 #fn2"><personName><givenNames>M.</givenNames><familyName>Golmohammadi</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1" corresponding="yes"><personName><givenNames>P.</givenNames><familyName>Llop</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a2"><personName><givenNames>G.</givenNames><familyName>Scuderi</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a3"><personName><givenNames>I.</givenNames><familyName>Gell</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a4"><personName><givenNames>J. H.</givenNames><familyName>Graham</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a3" corresponding="yes"><personName><givenNames>J.</givenNames><familyName>Cubero</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="ES"><unparsedAffiliation>Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia, Spain</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="IT"><unparsedAffiliation>Department of Phytosanitary Sciences and Technologies (DISTEF), Plant Pathology section, University of Catania, 95123 Catania, Italy</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="ES"><unparsedAffiliation>Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de La Coruña km 7·5, Madrid, Spain</unparsedAffiliation></affiliation><affiliation xml:id="a4" countryCode="US"><unparsedAffiliation>University of Florida, Citrus Research and Education Center (CREC) 700 Experiment Station Road, Lake Alfred, FL 33850‐2299, USA</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">bacterial survival</keyword><keyword xml:id="k2">citrus bacterial canker</keyword><keyword xml:id="k3"><i>Citrus</i></keyword><keyword xml:id="k4">real‐time PCR</keyword><keyword xml:id="k5">viable bacteria</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><p>The purpose of this study was to assess the stability of mRNA and rRNA for evaluation of viability for <i>Xanthomonas citri</i> subsp. <i>citri</i> (Xcc). Total RNA from Xcc suspensions subjected to different stress treatments (high temperature or chemical treatment with sodium orthophenylphenate at different concentrations) was extracted at different time periods post‐treatment (0, 3, 24 and 48 h) and analysed by quantitative real‐time reverse transcription PCR (Q‐RT‐PCR). Primers were designed from selected fragments of rRNA and mRNA from genes involved in bacterial fitness, virulence or general metabolic mechanisms (<i>gumD</i>, <i>rpfB</i>, <i>avrBs2</i> and <i>gyrB</i>). After stress treatment, only a 445‐bp fragment from the <i>gumD</i> mRNA was detected in live Xcc cells specifically, whereas other RNA fragments, as well as DNA targets, were detected in both viable and nonviable cells. Statistical analyses demonstrated that the amount of some transcripts from genes involved in xanthan synthesis, pathogenicity factor regulation and DNA processing was significantly reduced after lethal treatments. The amplification of the 445‐bp product from <i>gumD</i> mRNA was demonstrated to be useful for the detection of viable Xcc; the product was detected specifically from viable bacteria on leaf and citrus fruit surfaces and in citrus canker lesions. Instability of long RNA fragments can be used as a practical tool for the study of survival of citrus canker bacteria or for diagnostic purposes when the presence of viable bacteria needs to be confirmed.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>mRNA from selected genes is useful for specific detection and quantification of viable Xanthomonas citri subsp. citri</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Detection of viable cells of Xanthomonas citrisubsp. citri</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>mRNA from selected genes is useful for specific detection and quantification of viable Xanthomonas citri subsp. citri</title></titleInfo><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Golmohammadi</namePart><affiliation>Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia, Spain</affiliation><description>Note: First and second authors contributed equally to the paper.</description><description>Current address: Department of Plant Protection, Iran Citrus Research Institute, Ramsar, Iran.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Llop</namePart><affiliation>Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia, Spain</affiliation><description>Correspondence: E‐mail: </description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Scuderi</namePart><affiliation>Department of Phytosanitary Sciences and Technologies (DISTEF), Plant Pathology section, University of Catania, 95123 Catania, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I.</namePart><namePart type="family">Gell</namePart><affiliation>Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de La Coruña km 7·5, Madrid, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. H.</namePart><namePart type="family">Graham</namePart><affiliation>University of Florida, Citrus Research and Education Center (CREC) 700 Experiment Station Road, Lake Alfred, FL 33850‐2299, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Cubero</namePart><affiliation>Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de La Coruña km 7·5, Madrid, Spain</affiliation><description>Correspondence: E‐mail: </description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2012-06</dateIssued><edition>Published online 14 August 2011</edition><copyrightDate encoding="w3cdtf">2012</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">2</extent><extent unit="tables">3</extent></physicalDescription><abstract lang="en">The purpose of this study was to assess the stability of mRNA and rRNA for evaluation of viability for Xanthomonas citri subsp. citri (Xcc). Total RNA from Xcc suspensions subjected to different stress treatments (high temperature or chemical treatment with sodium orthophenylphenate at different concentrations) was extracted at different time periods post‐treatment (0, 3, 24 and 48 h) and analysed by quantitative real‐time reverse transcription PCR (Q‐RT‐PCR). Primers were designed from selected fragments of rRNA and mRNA from genes involved in bacterial fitness, virulence or general metabolic mechanisms (gumD, rpfB, avrBs2 and gyrB). After stress treatment, only a 445‐bp fragment from the gumD mRNA was detected in live Xcc cells specifically, whereas other RNA fragments, as well as DNA targets, were detected in both viable and nonviable cells. Statistical analyses demonstrated that the amount of some transcripts from genes involved in xanthan synthesis, pathogenicity factor regulation and DNA processing was significantly reduced after lethal treatments. The amplification of the 445‐bp product from gumD mRNA was demonstrated to be useful for the detection of viable Xcc; the product was detected specifically from viable bacteria on leaf and citrus fruit surfaces and in citrus canker lesions. Instability of long RNA fragments can be used as a practical tool for the study of survival of citrus canker bacteria or for diagnostic purposes when the presence of viable bacteria needs to be confirmed.</abstract><subject lang="en"><genre>keywords</genre><topic>bacterial survival</topic><topic>citrus bacterial canker</topic><topic>Citrus</topic><topic>real‐time PCR</topic><topic>viable bacteria</topic></subject><relatedItem type="host"><titleInfo><title>Plant Pathology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0032-0862</identifier><identifier type="eISSN">1365-3059</identifier><identifier type="DOI">10.1111/(ISSN)1365-3059</identifier><identifier type="PublisherID">PPA</identifier><part><date>2012</date><detail type="volume"><caption>vol.</caption><number>61</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>479</start><end>488</end><total>10</total></extent></part></relatedItem><identifier type="istex">3360682130BACCA3166C8B91442BE69FF5F117E1</identifier><identifier type="DOI">10.1111/j.1365-3059.2011.02526.x</identifier><identifier type="ArticleID">PPA2526</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2011 The Authors. Plant Pathology © 2011 BSPP</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><enrichments><json:item><type>multicat</type><uri>https://api.istex.fr/document/3360682130BACCA3166C8B91442BE69FF5F117E1/enrichments/multicat</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001936 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 001936 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:3360682130BACCA3166C8B91442BE69FF5F117E1
|texte= mRNA from selected genes is useful for specific detection and quantification of viable Xanthomonas citri subsp. citri
}}
| This area was generated with Dilib version V0.6.25. |  |