Calmodulin from Citrus sinensis : Purification and characterization
Identifieur interne : 001536 ( Istex/Checkpoint ); précédent : 001535; suivant : 001537Calmodulin from Citrus sinensis : Purification and characterization
Auteurs : Ian A. Dubery [Afrique du Sud] ; Johannes C. Schabort [Afrique du Sud]Source :
- Phytochemistry [ 0031-9422 ] ; 1987.
Abstract
A protein identifiable as calmodulin has been isolated from the fruit tissue of Citrus sinensis (cv. Valencia). This protein is relatively heat-stable, binds to hydrophobic gels in a calcium-dependent fashion and to antibody against rat testis calmodulin, and stimulates calmodulin-deficient cAMP phosphodiesterase. The protein has an isoelectric point of 3.7 and Mrs of 14500 and 17000 in the presence and absence of Ca2+, respectively. Based on its migration on SDS-PAGE gels, its UV absorption spectrum and its amino acid composition, calmodulin from citrus fruit is essentially identical to calmodulin isolated from other plant tissues, e.g. leaves and seeds.
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DOI: 10.1016/S0031-9422(00)81476-4
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