Risk of iatrogenic human immunodeficiency virus infection through transfusion of blood tested by inappropriately stored or expired rapid antibody assays in a Zambian hospital
Identifieur interne : 003C93 ( Istex/Corpus ); précédent : 003C92; suivant : 003C94Risk of iatrogenic human immunodeficiency virus infection through transfusion of blood tested by inappropriately stored or expired rapid antibody assays in a Zambian hospital
Auteurs : E. C. J. Consten ; J. T. M. Van Der Meer ; F. De Wolf ; H. A. Heij ; P. C. Henny ; J. J. B. Van LanschotSource :
- Transfusion [ 0041-1132 ] ; 1997-09.
English descriptors
- KwdEn :
- Allogeneic, Allogeneic blood, Allogeneic blood transfusion, Allogeneic blood transfusions, Antibody assays, Assay, Associate professor, Blood donors, Blood transfusion practices, Blood transfusions, Comparative evaluation, Data analysis, Donor, Donor blood, Donor population, Francis hospital, Iatrogenic, Infectious diseases, Internal medicine, Laboratory staff, Management board, Medical centre, Nonexpired, Nonexpired tests, Present study, Rapid antibody assays, Reagent, Room temperature, Seropositive samples, Seroprevalence, Serum samples, Single unit, Statistical analysis, Surgical, Surgical patients, Test results, Transfusion, Transfusion volume, Tropical medicine, University hospital, Zambia.
- Teeft :
- Allogeneic, Allogeneic blood, Allogeneic blood transfusion, Allogeneic blood transfusions, Antibody assays, Assay, Associate professor, Blood donors, Blood transfusion practices, Blood transfusions, Comparative evaluation, Data analysis, Donor, Donor blood, Donor population, Francis hospital, Iatrogenic, Infectious diseases, Internal medicine, Laboratory staff, Management board, Medical centre, Nonexpired, Nonexpired tests, Present study, Rapid antibody assays, Reagent, Room temperature, Seropositive samples, Seroprevalence, Serum samples, Single unit, Statistical analysis, Surgical, Surgical patients, Test results, Transfusion, Transfusion volume, Tropical medicine, University hospital, Zambia.
Abstract
BACKGROUND: The purpose of this study was to estimate the risk of human immunodeficiency virus (HIV) infection via the transfusion of blood tested by inappropriately stored or expired rapid antibody assays in Zambia. STUDY DESIGN AND METHODS: Surgical patients (n = 370) were tested with antibody assays (HIV‐spot and HIV 1+2) that had expired 3 to 6 months previously. Blood donors (n = 211) were tested by inappropriately stored but non‐expired HIV‐spot assay. Serum samples from both groups were retested with enzyme immunoassays, and the seropositivity of samples was confirmed by immunoblotting. RESULTS: Seroprevalence in surgical patients and blood donors was 19.8 and 11.6 percent, respectively. Sensitivity and specificity of HIV‐spot (expired) were 88.2 and 98.1 percent; those of HIV 1+2 (expired) were 82.1 and 94.7 percent; and those of HIV‐spot (non‐expired) were 91.7 and 98.8 percent, respectively. The risk of HIV infection via the transfusion of blood tested by HIV‐spot (expired), HIV‐spot (nonexpired), or HIV 1+2 (expired) was calculated to be 1.4, 1.0, and 3.2 percent, respectively. CONCLUSION: Manufacturers of the HIV‐spot and HIV 1+2 assays claim sensitivity and specificity of 98.8 and 100 percent and 100 and 99.5 percent, respectively. In this study, sensitivity and specificity were 11 to 18 percent lower. Moreover, in‐ date reagents also performed less well than the manufacturers claimed, but the worst results were with expired or improperly stored reagents. According to the manufacturers of HIV‐spot and HIV 1+2, the risk of HIV infection would be 0.2 and 0 percent, respectively. However, the risk of contracting HIV through transfusion is at least six times higher than expected.
Url:
DOI: 10.1046/j.1537-2995.1997.37997454020.x
Links to Exploration step
ISTEX:BA5BC5B314CF8B8C3EDCA340DF200692149EF24ELe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Risk of iatrogenic human immunodeficiency virus infection through transfusion of blood tested by inappropriately stored or expired rapid antibody assays in a Zambian hospital</title><author><name sortKey="Consten, E C J" sort="Consten, E C J" uniqKey="Consten E" first="E. C. J." last="Consten">E. C. J. Consten</name></author><author><name sortKey="Van Der Meer, J T M" sort="Van Der Meer, J T M" uniqKey="Van Der Meer J" first="J. T. M." last="Van Der Meer">J. T. M. Van Der Meer</name></author><author><name sortKey="De Wolf, F" sort="De Wolf, F" uniqKey="De Wolf F" first="F." last="De Wolf">F. De Wolf</name></author><author><name sortKey="Heij, H A" sort="Heij, H A" uniqKey="Heij H" first="H. A." last="Heij">H. A. Heij</name></author><author><name sortKey="Henny, P C" sort="Henny, P C" uniqKey="Henny P" first="P. C." last="Henny">P. C. Henny</name></author><author><name sortKey="Van Lanschot, J J B" sort="Van Lanschot, J J B" uniqKey="Van Lanschot J" first="J. J. B." last="Van Lanschot">J. J. B. Van Lanschot</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:BA5BC5B314CF8B8C3EDCA340DF200692149EF24E</idno><date when="1997" year="1997">1997</date><idno type="doi">10.1046/j.1537-2995.1997.37997454020.x</idno><idno type="url">https://api.istex.fr/document/BA5BC5B314CF8B8C3EDCA340DF200692149EF24E/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">003C93</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">003C93</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Risk of iatrogenic human immunodeficiency virus infection through transfusion of blood tested by inappropriately stored or expired rapid antibody assays in a Zambian hospital</title><author><name sortKey="Consten, E C J" sort="Consten, E C J" uniqKey="Consten E" first="E. C. J." last="Consten">E. C. J. Consten</name></author><author><name sortKey="Van Der Meer, J T M" sort="Van Der Meer, J T M" uniqKey="Van Der Meer J" first="J. T. M." last="Van Der Meer">J. T. M. Van Der Meer</name></author><author><name sortKey="De Wolf, F" sort="De Wolf, F" uniqKey="De Wolf F" first="F." last="De Wolf">F. De Wolf</name></author><author><name sortKey="Heij, H A" sort="Heij, H A" uniqKey="Heij H" first="H. A." last="Heij">H. A. Heij</name></author><author><name sortKey="Henny, P C" sort="Henny, P C" uniqKey="Henny P" first="P. C." last="Henny">P. C. Henny</name></author><author><name sortKey="Van Lanschot, J J B" sort="Van Lanschot, J J B" uniqKey="Van Lanschot J" first="J. J. B." last="Van Lanschot">J. J. B. Van Lanschot</name></author></analytic><monogr></monogr><series><title level="j" type="main">Transfusion</title><title level="j" type="alt">TRANSFUSION</title><idno type="ISSN">0041-1132</idno><idno type="eISSN">1537-2995</idno><imprint><biblScope unit="vol">37</biblScope><biblScope unit="issue">9</biblScope><biblScope unit="page" from="930">930</biblScope><biblScope unit="page" to="934">934</biblScope><biblScope unit="page-count">5</biblScope><publisher>Blackwell Science Ltd</publisher><pubPlace>Edinburgh, UK</pubPlace><date type="published" when="1997-09">1997-09</date></imprint><idno type="ISSN">0041-1132</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0041-1132</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Allogeneic</term><term>Allogeneic blood</term><term>Allogeneic blood transfusion</term><term>Allogeneic blood transfusions</term><term>Antibody assays</term><term>Assay</term><term>Associate professor</term><term>Blood donors</term><term>Blood transfusion practices</term><term>Blood transfusions</term><term>Comparative evaluation</term><term>Data analysis</term><term>Donor</term><term>Donor blood</term><term>Donor population</term><term>Francis hospital</term><term>Iatrogenic</term><term>Infectious diseases</term><term>Internal medicine</term><term>Laboratory staff</term><term>Management board</term><term>Medical centre</term><term>Nonexpired</term><term>Nonexpired tests</term><term>Present study</term><term>Rapid antibody assays</term><term>Reagent</term><term>Room temperature</term><term>Seropositive samples</term><term>Seroprevalence</term><term>Serum samples</term><term>Single unit</term><term>Statistical analysis</term><term>Surgical</term><term>Surgical patients</term><term>Test results</term><term>Transfusion</term><term>Transfusion volume</term><term>Tropical medicine</term><term>University hospital</term><term>Zambia</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Allogeneic</term><term>Allogeneic blood</term><term>Allogeneic blood transfusion</term><term>Allogeneic blood transfusions</term><term>Antibody assays</term><term>Assay</term><term>Associate professor</term><term>Blood donors</term><term>Blood transfusion practices</term><term>Blood transfusions</term><term>Comparative evaluation</term><term>Data analysis</term><term>Donor</term><term>Donor blood</term><term>Donor population</term><term>Francis hospital</term><term>Iatrogenic</term><term>Infectious diseases</term><term>Internal medicine</term><term>Laboratory staff</term><term>Management board</term><term>Medical centre</term><term>Nonexpired</term><term>Nonexpired tests</term><term>Present study</term><term>Rapid antibody assays</term><term>Reagent</term><term>Room temperature</term><term>Seropositive samples</term><term>Seroprevalence</term><term>Serum samples</term><term>Single unit</term><term>Statistical analysis</term><term>Surgical</term><term>Surgical patients</term><term>Test results</term><term>Transfusion</term><term>Transfusion volume</term><term>Tropical medicine</term><term>University hospital</term><term>Zambia</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">BACKGROUND: The purpose of this study was to estimate the risk of human immunodeficiency virus (HIV) infection via the transfusion of blood tested by inappropriately stored or expired rapid antibody assays in Zambia. STUDY DESIGN AND METHODS: Surgical patients (n = 370) were tested with antibody assays (HIV‐spot and HIV 1+2) that had expired 3 to 6 months previously. Blood donors (n = 211) were tested by inappropriately stored but non‐expired HIV‐spot assay. Serum samples from both groups were retested with enzyme immunoassays, and the seropositivity of samples was confirmed by immunoblotting. RESULTS: Seroprevalence in surgical patients and blood donors was 19.8 and 11.6 percent, respectively. Sensitivity and specificity of HIV‐spot (expired) were 88.2 and 98.1 percent; those of HIV 1+2 (expired) were 82.1 and 94.7 percent; and those of HIV‐spot (non‐expired) were 91.7 and 98.8 percent, respectively. The risk of HIV infection via the transfusion of blood tested by HIV‐spot (expired), HIV‐spot (nonexpired), or HIV 1+2 (expired) was calculated to be 1.4, 1.0, and 3.2 percent, respectively. CONCLUSION: Manufacturers of the HIV‐spot and HIV 1+2 assays claim sensitivity and specificity of 98.8 and 100 percent and 100 and 99.5 percent, respectively. In this study, sensitivity and specificity were 11 to 18 percent lower. Moreover, in‐ date reagents also performed less well than the manufacturers claimed, but the worst results were with expired or improperly stored reagents. According to the manufacturers of HIV‐spot and HIV 1+2, the risk of HIV infection would be 0.2 and 0 percent, respectively. However, the risk of contracting HIV through transfusion is at least six times higher than expected.</div></front></TEI><istex><corpusName>wiley</corpusName><keywords><teeft><json:string>transfusion</json:string><json:string>iatrogenic</json:string><json:string>seroprevalence</json:string><json:string>assay</json:string><json:string>allogeneic</json:string><json:string>nonexpired</json:string><json:string>zambia</json:string><json:string>antibody assays</json:string><json:string>serum samples</json:string><json:string>allogeneic blood transfusion</json:string><json:string>surgical patients</json:string><json:string>reagent</json:string><json:string>donor population</json:string><json:string>blood donors</json:string><json:string>francis hospital</json:string><json:string>associate professor</json:string><json:string>surgical</json:string><json:string>nonexpired tests</json:string><json:string>donor blood</json:string><json:string>present study</json:string><json:string>allogeneic blood</json:string><json:string>seropositive samples</json:string><json:string>transfusion volume</json:string><json:string>laboratory staff</json:string><json:string>medical centre</json:string><json:string>donor</json:string><json:string>room temperature</json:string><json:string>statistical analysis</json:string><json:string>internal medicine</json:string><json:string>data analysis</json:string><json:string>infectious diseases</json:string><json:string>single unit</json:string><json:string>rapid antibody assays</json:string><json:string>tropical medicine</json:string><json:string>management board</json:string><json:string>university hospital</json:string><json:string>test results</json:string><json:string>blood transfusions</json:string><json:string>comparative evaluation</json:string><json:string>blood transfusion practices</json:string><json:string>allogeneic blood transfusions</json:string></teeft></keywords><author><json:item><name>E.C.J. Consten MD</name></json:item><json:item><name>J.T.M. van der Meer MD</name></json:item><json:item><name>F. de Wolf MD</name></json:item><json:item><name>H.A. Heij MD</name></json:item><json:item><name>P.C. Henny MD</name></json:item><json:item><name>J.J. B. van Lanschot MD</name></json:item></author><articleId><json:string>TRF5351</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>miscellaneous</json:string></originalGenre><abstract>BACKGROUND: The purpose of this study was to estimate the risk of human immunodeficiency virus (HIV) infection via the transfusion of blood tested by inappropriately stored or expired rapid antibody assays in Zambia. STUDY DESIGN AND METHODS: Surgical patients (n = 370) were tested with antibody assays (HIV‐spot and HIV 1+2) that had expired 3 to 6 months previously. Blood donors (n = 211) were tested by inappropriately stored but non‐expired HIV‐spot assay. Serum samples from both groups were retested with enzyme immunoassays, and the seropositivity of samples was confirmed by immunoblotting. RESULTS: Seroprevalence in surgical patients and blood donors was 19.8 and 11.6 percent, respectively. Sensitivity and specificity of HIV‐spot (expired) were 88.2 and 98.1 percent; those of HIV 1+2 (expired) were 82.1 and 94.7 percent; and those of HIV‐spot (non‐expired) were 91.7 and 98.8 percent, respectively. The risk of HIV infection via the transfusion of blood tested by HIV‐spot (expired), HIV‐spot (nonexpired), or HIV 1+2 (expired) was calculated to be 1.4, 1.0, and 3.2 percent, respectively. CONCLUSION: Manufacturers of the HIV‐spot and HIV 1+2 assays claim sensitivity and specificity of 98.8 and 100 percent and 100 and 99.5 percent, respectively. In this study, sensitivity and specificity were 11 to 18 percent lower. Moreover, in‐ date reagents also performed less well than the manufacturers claimed, but the worst results were with expired or improperly stored reagents. According to the manufacturers of HIV‐spot and HIV 1+2, the risk of HIV infection would be 0.2 and 0 percent, respectively. However, the risk of contracting HIV through transfusion is at least six times higher than expected.</abstract><qualityIndicators><score>6.498</score><pdfVersion>1.4</pdfVersion><pdfPageSize>612 x 827.759 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>1717</abstractCharCount><pdfWordCount>2998</pdfWordCount><pdfCharCount>19170</pdfCharCount><pdfPageCount>5</pdfPageCount><abstractWordCount>264</abstractWordCount></qualityIndicators><title>Risk of iatrogenic human immunodeficiency virus infection through transfusion of blood tested by inappropriately stored or expired rapid antibody assays in a Zambian hospital</title><genre><json:string>other</json:string></genre><host><title>Transfusion</title><language><json:string>unknown</json:string></language><doi><json:string>10.1111/(ISSN)1537-2995</json:string></doi><issn><json:string>0041-1132</json:string></issn><eissn><json:string>1537-2995</json:string></eissn><publisherId><json:string>TRF</json:string></publisherId><volume>37</volume><issue>9</issue><pages><first>930</first><last>934</last><total>5</total></pages><genre><json:string>journal</json:string></genre></host><categories><wos><json:string>science</json:string><json:string>hematology</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>clinical medicine</json:string><json:string>cardiovascular system & hematology</json:string></scienceMetrix><inist><json:string>sciences appliquees, technologies et medecines</json:string><json:string>sciences biologiques et medicales</json:string><json:string>sciences medicales</json:string></inist></categories><publicationDate>1997</publicationDate><copyrightDate>1997</copyrightDate><doi><json:string>10.1046/j.1537-2995.1997.37997454020.x</json:string></doi><id>BA5BC5B314CF8B8C3EDCA340DF200692149EF24E</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/BA5BC5B314CF8B8C3EDCA340DF200692149EF24E/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/BA5BC5B314CF8B8C3EDCA340DF200692149EF24E/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/BA5BC5B314CF8B8C3EDCA340DF200692149EF24E/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main">Risk of iatrogenic human immunodeficiency virus infection through transfusion of blood tested by inappropriately stored or expired rapid antibody assays in a Zambian hospital</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Science Ltd</publisher><pubPlace>Edinburgh, UK</pubPlace><availability><licence>1997 AABB</licence></availability><date type="published" when="1997-09"></date></publicationStmt><notesStmt><note type="content-type" subtype="other" source="miscellaneous" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-7474895G-0">other</note><note type="publication-type" subtype="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="other"><analytic><title level="a" type="main">Risk of iatrogenic human immunodeficiency virus infection through transfusion of blood tested by inappropriately stored or expired rapid antibody assays in a Zambian hospital</title><author xml:id="author-0000"><persName><forename type="first">E.C.J.</forename><surname>Consten</surname><roleName type="degree">MD</roleName></persName></author><author xml:id="author-0001"><persName><forename type="first">J.T.M.</forename><surname>Meer</surname><roleName type="degree">MD</roleName></persName></author><author xml:id="author-0002"><persName><forename type="first">F.</forename><surname>Wolf</surname><roleName type="degree">MD</roleName></persName></author><author xml:id="author-0003"><persName><forename type="first">H.A.</forename><surname>Heij</surname><roleName type="degree">MD</roleName></persName></author><author xml:id="author-0004"><persName><forename type="first">P.C.</forename><surname>Henny</surname><roleName type="degree">MD</roleName></persName></author><author xml:id="author-0005"><persName><forename type="first">J.J. B.</forename><surname>Lanschot</surname><roleName type="degree">MD</roleName></persName></author><idno type="istex">BA5BC5B314CF8B8C3EDCA340DF200692149EF24E</idno><idno type="ark">ark:/67375/WNG-2LKMLM45-9</idno><idno type="DOI">10.1046/j.1537-2995.1997.37997454020.x</idno><idno type="unit">TRF5351</idno><idno type="toTypesetVersion">file:TRF.TRF5351.pdf</idno></analytic><monogr><title level="j" type="main">Transfusion</title><title level="j" type="alt">TRANSFUSION</title><idno type="pISSN">0041-1132</idno><idno type="eISSN">1537-2995</idno><idno type="book-DOI">10.1111/(ISSN)1537-2995</idno><idno type="book-part-DOI">10.1111/trf.1997.37.issue-9</idno><idno type="product">TRF</idno><idno type="publisherDivision">ST</idno><imprint><biblScope unit="vol">37</biblScope><biblScope unit="issue">9</biblScope><biblScope unit="page" from="930">930</biblScope><biblScope unit="page" to="934">934</biblScope><biblScope unit="page-count">5</biblScope><publisher>Blackwell Science Ltd</publisher><pubPlace>Edinburgh, UK</pubPlace><date type="published" when="1997-09"></date></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><abstract xml:lang="en" style="main"><p>BACKGROUND: The purpose of this study was to estimate the risk of human immunodeficiency virus (HIV) infection via the transfusion of blood tested by inappropriately stored or expired rapid antibody assays in Zambia. STUDY DESIGN AND METHODS: Surgical patients (n = 370) were tested with antibody assays (HIV‐spot and HIV 1+2) that had expired 3 to 6 months previously. Blood donors (n = 211) were tested by inappropriately stored but non‐expired HIV‐spot assay. Serum samples from both groups were retested with enzyme immunoassays, and the seropositivity of samples was confirmed by immunoblotting. RESULTS: Seroprevalence in surgical patients and blood donors was 19.8 and 11.6 percent, respectively. Sensitivity and specificity of HIV‐spot (expired) were 88.2 and 98.1 percent; those of HIV 1+2 (expired) were 82.1 and 94.7 percent; and those of HIV‐spot (non‐expired) were 91.7 and 98.8 percent, respectively. The risk of HIV infection via the transfusion of blood tested by HIV‐spot (expired), HIV‐spot (nonexpired), or HIV 1+2 (expired) was calculated to be 1.4, 1.0, and 3.2 percent, respectively. CONCLUSION: Manufacturers of the HIV‐spot and HIV 1+2 assays claim sensitivity and specificity of 98.8 and 100 percent and 100 and 99.5 percent, respectively. In this study, sensitivity and specificity were 11 to 18 percent lower. Moreover, in‐ date reagents also performed less well than the manufacturers claimed, but the worst results were with expired or improperly stored reagents. According to the manufacturers of HIV‐spot and HIV 1+2, the risk of HIV infection would be 0.2 and 0 percent, respectively. However, the risk of contracting HIV through transfusion is at least six times higher than expected.</p></abstract><textClass><keywords rend="tocHeading1"><term>Transfusion Complications</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/BA5BC5B314CF8B8C3EDCA340DF200692149EF24E/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Science Ltd</publisherName><publisherLoc>Edinburgh, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1537-2995</doi><issn type="print">0041-1132</issn><issn type="electronic">1537-2995</issn><idGroup><id type="product" value="TRF"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="TRANSFUSION">Transfusion</title></titleGroup></publicationMeta><publicationMeta level="part" position="09009"><doi origin="wiley">10.1111/trf.1997.37.issue-9</doi><numberingGroup><numbering type="journalVolume" number="37">37</numbering><numbering type="journalIssue" number="9">9</numbering></numberingGroup><coverDate startDate="1997-09">September 1997</coverDate></publicationMeta><publicationMeta level="unit" type="miscellaneous" position="10" status="forIssue"><doi origin="wiley">10.1046/j.1537-2995.1997.37997454020.x</doi><idGroup><id type="unit" value="TRF5351"></id></idGroup><countGroup><count type="pageTotal" number="5"></count></countGroup><titleGroup><title type="tocHeading1">Transfusion Complications</title></titleGroup><copyright>1997 AABB</copyright><eventGroup><event type="firstOnline" date="2003-02-27"></event><event type="publishedOnlineFinalForm" date="2003-02-27"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-03-03"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-02-10"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-11-04"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="930">930</numbering><numbering type="pageLast" number="934">934</numbering></numberingGroup><linkGroup><link type="toTypesetVersion" href="file:TRF.TRF5351.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received for publication September 6, 1995; revision received September 18, 1996, and accepted February 12, 1997.</unparsedEditorialHistory><countGroup><count type="referenceTotal" number="23"></count><count type="linksCrossRef" number="6"></count></countGroup><titleGroup><title type="main">Risk of iatrogenic human immunodeficiency virus infection through transfusion of blood tested by inappropriately stored or expired rapid antibody assays in a Zambian hospital</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" noteRef="#fn1"><personName><givenNames>E.C.J.</givenNames><familyName>Consten</familyName><degrees>MD</degrees></personName></creator><creator creatorRole="author" xml:id="cr2" noteRef="#fn2"><personName><givenNames>J.T.M.</givenNames><familyNamePrefix>van der</familyNamePrefix><familyName>Meer</familyName><degrees>MD</degrees></personName></creator><creator creatorRole="author" xml:id="cr3" noteRef="#fn3"><personName><givenNames>F.</givenNames><familyNamePrefix>de</familyNamePrefix><familyName>Wolf</familyName><degrees>MD</degrees></personName></creator><creator creatorRole="author" xml:id="cr4" noteRef="#fn4"><personName><givenNames>H.A.</givenNames><familyName>Heij</familyName><degrees>MD</degrees></personName></creator><creator creatorRole="author" xml:id="cr5" noteRef="#fn5"><personName><givenNames>P.C.</givenNames><familyName>Henny</familyName><degrees>MD</degrees></personName></creator><creator creatorRole="author" xml:id="cr6" noteRef="#fn6"><personName><givenNames>J.J. B.</givenNames><familyNamePrefix>van</familyNamePrefix><familyName>Lanschot</familyName><degrees>MD</degrees></personName></creator></creators><abstractGroup><abstract type="main" xml:lang="en"><p>BACKGROUND: The purpose of this study was to estimate the risk of human immunodeficiency virus (HIV) infection via the transfusion of blood tested by inappropriately stored or expired rapid antibody assays in Zambia. STUDY DESIGN AND METHODS: Surgical patients (n = 370) were tested with antibody assays (HIV‐spot and HIV 1+2) that had expired 3 to 6 months previously. Blood donors (n = 211) were tested by inappropriately stored but non‐expired HIV‐spot assay. Serum samples from both groups were retested with enzyme immunoassays, and the seropositivity of samples was confirmed by immunoblotting. RESULTS: Seroprevalence in surgical patients and blood donors was 19.8 and 11.6 percent, respectively. Sensitivity and specificity of HIV‐spot (expired) were 88.2 and 98.1 percent; those of HIV 1+2 (expired) were 82.1 and 94.7 percent; and those of HIV‐spot (non‐expired) were 91.7 and 98.8 percent, respectively. The risk of HIV infection via the transfusion of blood tested by HIV‐spot (expired), HIV‐spot (nonexpired), or HIV 1+2 (expired) was calculated to be 1.4, 1.0, and 3.2 percent, respectively. CONCLUSION: Manufacturers of the HIV‐spot and HIV 1+2 assays claim sensitivity and specificity of 98.8 and 100 percent and 100 and 99.5 percent, respectively. In this study, sensitivity and specificity were 11 to 18 percent lower. Moreover, in‐ date reagents also performed less well than the manufacturers claimed, but the worst results were with expired or improperly stored reagents. According to the manufacturers of HIV‐spot and HIV 1+2, the risk of HIV infection would be 0.2 and 0 percent, respectively. However, the risk of contracting HIV through transfusion is at least six times higher than expected.</p></abstract></abstractGroup></contentMeta><noteGroup><note xml:id="fn1"><label>1</label><p>E.C.J. Consten, MD, Surgery Resident, Department of Surgery (G4–105), Academic Medical Centre, Meibergdreef 9,1105 AZ Amsterdam, the Netherlands. [No reprints available]</p></note><note xml:id="fn2"><label>2</label><p>J.T.M. van der Meer, MD, Associate Professor, Subdivision of Infectious Diseases, Tropical Medicine and AIDS, Department of Internal Medicine, Academic Medical Centre.</p></note><note xml:id="fn3"><label>3</label><p>F. de Wolf, MD, Associate Professor, Laboratory of Human Retrovirology, Academic Medical Centre.</p></note><note xml:id="fn4"><label>4</label><p>H.A. Heij, MD, Associate Professor, Department of Paediatric Surgery, Academic Medical Centre; current address: Superintendant, St. Francis Hospital, Katete, Zambia.</p></note><note xml:id="fn5"><label>5</label><p>P.C. Henny, MD, Associate Professor, Department of Anaesthesiology, Academic Medical Centre.</p></note><note xml:id="fn6"><label>6</label><p>J.J. B. van Lanschot, MD, Associate Professor, Department of Surgery, Academic Medical Centre.</p></note></noteGroup></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Risk of iatrogenic human immunodeficiency virus infection through transfusion of blood tested by inappropriately stored or expired rapid antibody assays in a Zambian hospital</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Risk of iatrogenic human immunodeficiency virus infection through transfusion of blood tested by inappropriately stored or expired rapid antibody assays in a Zambian hospital</title></titleInfo><name type="personal"><namePart type="given">E.C.J.</namePart><namePart type="family">Consten</namePart><namePart type="termsOfAddress">MD</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.T.M.</namePart><namePart type="family">van der Meer</namePart><namePart type="termsOfAddress">MD</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F.</namePart><namePart type="family">de Wolf</namePart><namePart type="termsOfAddress">MD</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.A.</namePart><namePart type="family">Heij</namePart><namePart type="termsOfAddress">MD</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.C.</namePart><namePart type="family">Henny</namePart><namePart type="termsOfAddress">MD</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.J. B.</namePart><namePart type="family">van Lanschot</namePart><namePart type="termsOfAddress">MD</namePart><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="miscellaneous" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Blackwell Science Ltd</publisher><place><placeTerm type="text">Edinburgh, UK</placeTerm></place><dateIssued encoding="w3cdtf">1997-09</dateIssued><edition>Received for publication September 6, 1995; revision received September 18, 1996, and accepted February 12, 1997.</edition><copyrightDate encoding="w3cdtf">1997</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="references">23</extent><extent unit="linksCrossRef">6</extent></physicalDescription><abstract lang="en">BACKGROUND: The purpose of this study was to estimate the risk of human immunodeficiency virus (HIV) infection via the transfusion of blood tested by inappropriately stored or expired rapid antibody assays in Zambia. STUDY DESIGN AND METHODS: Surgical patients (n = 370) were tested with antibody assays (HIV‐spot and HIV 1+2) that had expired 3 to 6 months previously. Blood donors (n = 211) were tested by inappropriately stored but non‐expired HIV‐spot assay. Serum samples from both groups were retested with enzyme immunoassays, and the seropositivity of samples was confirmed by immunoblotting. RESULTS: Seroprevalence in surgical patients and blood donors was 19.8 and 11.6 percent, respectively. Sensitivity and specificity of HIV‐spot (expired) were 88.2 and 98.1 percent; those of HIV 1+2 (expired) were 82.1 and 94.7 percent; and those of HIV‐spot (non‐expired) were 91.7 and 98.8 percent, respectively. The risk of HIV infection via the transfusion of blood tested by HIV‐spot (expired), HIV‐spot (nonexpired), or HIV 1+2 (expired) was calculated to be 1.4, 1.0, and 3.2 percent, respectively. CONCLUSION: Manufacturers of the HIV‐spot and HIV 1+2 assays claim sensitivity and specificity of 98.8 and 100 percent and 100 and 99.5 percent, respectively. In this study, sensitivity and specificity were 11 to 18 percent lower. Moreover, in‐ date reagents also performed less well than the manufacturers claimed, but the worst results were with expired or improperly stored reagents. According to the manufacturers of HIV‐spot and HIV 1+2, the risk of HIV infection would be 0.2 and 0 percent, respectively. However, the risk of contracting HIV through transfusion is at least six times higher than expected.</abstract><relatedItem type="host"><titleInfo><title>Transfusion</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><identifier type="ISSN">0041-1132</identifier><identifier type="eISSN">1537-2995</identifier><identifier type="DOI">10.1111/(ISSN)1537-2995</identifier><identifier type="PublisherID">TRF</identifier><part><date>1997</date><detail type="volume"><caption>vol.</caption><number>37</number></detail><detail type="issue"><caption>no.</caption><number>9</number></detail><extent unit="pages"><start>930</start><end>934</end><total>5</total></extent></part></relatedItem><identifier type="istex">BA5BC5B314CF8B8C3EDCA340DF200692149EF24E</identifier><identifier type="ark">ark:/67375/WNG-2LKMLM45-9</identifier><identifier type="DOI">10.1046/j.1537-2995.1997.37997454020.x</identifier><identifier type="ArticleID">TRF5351</identifier><accessCondition type="use and reproduction" contentType="copyright">1997 AABB</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Blackwell Science Ltd</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/BA5BC5B314CF8B8C3EDCA340DF200692149EF24E/metadata/json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/SidaSubSaharaV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003C93 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 003C93 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= SidaSubSaharaV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:BA5BC5B314CF8B8C3EDCA340DF200692149EF24E
|texte= Risk of iatrogenic human immunodeficiency virus infection through transfusion of blood tested by inappropriately stored or expired rapid antibody assays in a Zambian hospital
}}
| This area was generated with Dilib version V0.6.32. |  |