Impact of Acyclovir on Genital and Plasma HIV-1 RNA, Genital Herpes Simplex Virus Type 2 DNA, and Ulcer Healing among HIV-1–Infected African Women with Herpes Ulcers: A Randomized Placebo-Controlled Trial
Identifieur interne : 003752 ( Istex/Corpus ); précédent : 003751; suivant : 003753Impact of Acyclovir on Genital and Plasma HIV-1 RNA, Genital Herpes Simplex Virus Type 2 DNA, and Ulcer Healing among HIV-1–Infected African Women with Herpes Ulcers: A Randomized Placebo-Controlled Trial
Auteurs : Philippe Mayaud ; Jérôme Legoff ; Helen A. Weiss ; Gérard Grésenguet ; Khonde Nzambi ; Hicham Bouhlal ; Eric Frost ; Jacques Pépin ; Jean-Elie Malkin ; Richard J. Hayes ; David C. W. Mabey ; Laurent BélecSource :
- The Journal of Infectious Diseases [ 0022-1899 ] ; 2009-07-15.
Abstract
BackgroundLittle is known about the impact of episodic treatment of herpes on human immunodeficiency virus type 1 (HIV-1) MethodsWomen from Ghana and the Central African Republic who had genital ulcers were enrolled in a randomized, double-blind, placebo-controlled trial of acyclovir plus antibacterials and were monitored for 28 days. Ulcer etiologies and detection of lesional HIV-1 RNA were determined by polymerase chain reaction (PCR). Cervicovaginal HIV-1 RNA and herpes simplex virus type 2 (HSV-2) DNA and plasma HIV-1 RNA were quantitated by real-time PCR. Primary analyses included 118 HIV-1–infected women with HSV-2 ulcers (54 of whom were given acyclovir and 64 of whom were given placebo) ResultsAcyclovir had little impact on (1) detection of cervicovaginal HIV-1 RNA (risk ratio [RR], 0.96; 95% confidence interval [CI], 0.8–1.2) at day 7 of treatment, (2) the mean cervicovaginal HIV-1 RNA load (−0.06 log10 copies/mL; 95% CI, −0.4 to 0.3 log10 copies/mL) at day 7 of treatment, or (3) the plasma HIV-1 RNA load (+0.09 log10 copies/mL; 95% CI, −0.1 to 0.3 log10 copies/mL) at day 14 of treatment. At day 7, women receiving acyclovir were less likely to have detectable lesional HIV-1 RNA (RR, 0.70; 95% CI, 0.4–1.2) or cervicovaginal HSV-2 DNA (RR, 0.69; 95% CI, 0.4–1.3), had a lower quantity of HSV-2 DNA (−0.99 log10 copies/mL; 95% CI, −1.8 to −0.2 log10 copies/mL), and were more likely to have a healed ulcer (RR, 1.26; 95% CI, 0.9–1.9) ConclusionEpisodic therapy for herpes reduced the quantity of cervicovaginal HSV-2 DNA and slightly improved ulcer healing, but it did not decrease genital and plasma HIV-1 RNA loads Trial registrationClinicalTrials.gov identifier NCT00158483
Url:
DOI: 10.1086/599991
Links to Exploration step
ISTEX:A8D7F03BC8675B8AACE47022187702C7B7DE22EELe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Impact of Acyclovir on Genital and Plasma HIV-1 RNA, Genital Herpes Simplex Virus Type 2 DNA, and Ulcer Healing among HIV-1–Infected African Women with Herpes Ulcers: A Randomized Placebo-Controlled Trial</title>
<author><name sortKey="Mayaud, Philippe" sort="Mayaud, Philippe" uniqKey="Mayaud P" first="Philippe" last="Mayaud">Philippe Mayaud</name>
<affiliation><mods:affiliation>Clinical Research Unit, Department of Infectious and Tropical Diseases, and</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>Reprints or correspondence: Philippe Mayaud, Clinical Research Unit, London School of Hygiene & Tropical Medicine, Keppel St., London WC1E 7HT, UK (philippe.mayaud@lshtm.ac.uk)</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Legoff, Jerome" sort="Legoff, Jerome" uniqKey="Legoff J" first="Jérôme" last="Legoff">Jérôme Legoff</name>
<affiliation><mods:affiliation>Université Paris Descartes, Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743 (Immunologie Humaine), Centre de Recherches Biomédicales des Cordeliers and Laboratoire de Virologie, Hôpital Européen Georges Pompidou,</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>Université Paris Diderot, Laboratoire de Microbiologie, Hôpital Saint-Louis, and</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Weiss, Helen A" sort="Weiss, Helen A" uniqKey="Weiss H" first="Helen A." last="Weiss">Helen A. Weiss</name>
<affiliation><mods:affiliation>Infectious Disease Epidemiology Unit, Department of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, London, United Kingdom;</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Gresenguet, Gerard" sort="Gresenguet, Gerard" uniqKey="Gresenguet G" first="Gérard" last="Grésenguet">Gérard Grésenguet</name>
<affiliation><mods:affiliation>Centre National de Référence des Maladies Sexuellement Transmissibles et du SIDA de Bangui and Unité de Recherches et d’Intervention sur les Maladies Sexuellement Transmissibles et du SIDA, Faculté des Sciences de la Santé, Bangui, Central African Republic;</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Nzambi, Khonde" sort="Nzambi, Khonde" uniqKey="Nzambi K" first="Khonde" last="Nzambi">Khonde Nzambi</name>
<affiliation><mods:affiliation>West African Project To Combat AIDS and STIs, Accra, Ghana;</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Bouhlal, Hicham" sort="Bouhlal, Hicham" uniqKey="Bouhlal H" first="Hicham" last="Bouhlal">Hicham Bouhlal</name>
<affiliation><mods:affiliation>Université Paris Descartes, Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743 (Immunologie Humaine), Centre de Recherches Biomédicales des Cordeliers and Laboratoire de Virologie, Hôpital Européen Georges Pompidou,</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Frost, Eric" sort="Frost, Eric" uniqKey="Frost E" first="Eric" last="Frost">Eric Frost</name>
<affiliation><mods:affiliation>Centre for International Health, University of Sherbrooke, Sherbrooke, Canada</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Pepin, Jacques" sort="Pepin, Jacques" uniqKey="Pepin J" first="Jacques" last="Pépin">Jacques Pépin</name>
<affiliation><mods:affiliation>Centre for International Health, University of Sherbrooke, Sherbrooke, Canada</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Malkin, Jean Elie" sort="Malkin, Jean Elie" uniqKey="Malkin J" first="Jean-Elie" last="Malkin">Jean-Elie Malkin</name>
<affiliation><mods:affiliation>Centre Médical, Institut Pasteur, Paris, France;</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Hayes, Richard J" sort="Hayes, Richard J" uniqKey="Hayes R" first="Richard J." last="Hayes">Richard J. Hayes</name>
<affiliation><mods:affiliation>Infectious Disease Epidemiology Unit, Department of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, London, United Kingdom;</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Mabey, David C W" sort="Mabey, David C W" uniqKey="Mabey D" first="David C. W." last="Mabey">David C. W. Mabey</name>
<affiliation><mods:affiliation>Clinical Research Unit, Department of Infectious and Tropical Diseases, and</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Belec, Laurent" sort="Belec, Laurent" uniqKey="Belec L" first="Laurent" last="Bélec">Laurent Bélec</name>
<affiliation><mods:affiliation>Université Paris Descartes, Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743 (Immunologie Humaine), Centre de Recherches Biomédicales des Cordeliers and Laboratoire de Virologie, Hôpital Européen Georges Pompidou,</mods:affiliation>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:A8D7F03BC8675B8AACE47022187702C7B7DE22EE</idno>
<date when="2009" year="2009">2009</date>
<idno type="doi">10.1086/599991</idno>
<idno type="url">https://api.istex.fr/document/A8D7F03BC8675B8AACE47022187702C7B7DE22EE/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">003752</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">003752</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Impact of Acyclovir on Genital and Plasma HIV-1 RNA, Genital Herpes Simplex Virus Type 2 DNA, and Ulcer Healing among HIV-1–Infected African Women with Herpes Ulcers: A Randomized Placebo-Controlled Trial</title>
<author><name sortKey="Mayaud, Philippe" sort="Mayaud, Philippe" uniqKey="Mayaud P" first="Philippe" last="Mayaud">Philippe Mayaud</name>
<affiliation><mods:affiliation>Clinical Research Unit, Department of Infectious and Tropical Diseases, and</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>Reprints or correspondence: Philippe Mayaud, Clinical Research Unit, London School of Hygiene & Tropical Medicine, Keppel St., London WC1E 7HT, UK (philippe.mayaud@lshtm.ac.uk)</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Legoff, Jerome" sort="Legoff, Jerome" uniqKey="Legoff J" first="Jérôme" last="Legoff">Jérôme Legoff</name>
<affiliation><mods:affiliation>Université Paris Descartes, Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743 (Immunologie Humaine), Centre de Recherches Biomédicales des Cordeliers and Laboratoire de Virologie, Hôpital Européen Georges Pompidou,</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>Université Paris Diderot, Laboratoire de Microbiologie, Hôpital Saint-Louis, and</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Weiss, Helen A" sort="Weiss, Helen A" uniqKey="Weiss H" first="Helen A." last="Weiss">Helen A. Weiss</name>
<affiliation><mods:affiliation>Infectious Disease Epidemiology Unit, Department of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, London, United Kingdom;</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Gresenguet, Gerard" sort="Gresenguet, Gerard" uniqKey="Gresenguet G" first="Gérard" last="Grésenguet">Gérard Grésenguet</name>
<affiliation><mods:affiliation>Centre National de Référence des Maladies Sexuellement Transmissibles et du SIDA de Bangui and Unité de Recherches et d’Intervention sur les Maladies Sexuellement Transmissibles et du SIDA, Faculté des Sciences de la Santé, Bangui, Central African Republic;</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Nzambi, Khonde" sort="Nzambi, Khonde" uniqKey="Nzambi K" first="Khonde" last="Nzambi">Khonde Nzambi</name>
<affiliation><mods:affiliation>West African Project To Combat AIDS and STIs, Accra, Ghana;</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Bouhlal, Hicham" sort="Bouhlal, Hicham" uniqKey="Bouhlal H" first="Hicham" last="Bouhlal">Hicham Bouhlal</name>
<affiliation><mods:affiliation>Université Paris Descartes, Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743 (Immunologie Humaine), Centre de Recherches Biomédicales des Cordeliers and Laboratoire de Virologie, Hôpital Européen Georges Pompidou,</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Frost, Eric" sort="Frost, Eric" uniqKey="Frost E" first="Eric" last="Frost">Eric Frost</name>
<affiliation><mods:affiliation>Centre for International Health, University of Sherbrooke, Sherbrooke, Canada</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Pepin, Jacques" sort="Pepin, Jacques" uniqKey="Pepin J" first="Jacques" last="Pépin">Jacques Pépin</name>
<affiliation><mods:affiliation>Centre for International Health, University of Sherbrooke, Sherbrooke, Canada</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Malkin, Jean Elie" sort="Malkin, Jean Elie" uniqKey="Malkin J" first="Jean-Elie" last="Malkin">Jean-Elie Malkin</name>
<affiliation><mods:affiliation>Centre Médical, Institut Pasteur, Paris, France;</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Hayes, Richard J" sort="Hayes, Richard J" uniqKey="Hayes R" first="Richard J." last="Hayes">Richard J. Hayes</name>
<affiliation><mods:affiliation>Infectious Disease Epidemiology Unit, Department of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, London, United Kingdom;</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Mabey, David C W" sort="Mabey, David C W" uniqKey="Mabey D" first="David C. W." last="Mabey">David C. W. Mabey</name>
<affiliation><mods:affiliation>Clinical Research Unit, Department of Infectious and Tropical Diseases, and</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Belec, Laurent" sort="Belec, Laurent" uniqKey="Belec L" first="Laurent" last="Bélec">Laurent Bélec</name>
<affiliation><mods:affiliation>Université Paris Descartes, Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743 (Immunologie Humaine), Centre de Recherches Biomédicales des Cordeliers and Laboratoire de Virologie, Hôpital Européen Georges Pompidou,</mods:affiliation>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">The Journal of Infectious Diseases</title>
<title level="j" type="abbrev">The Journal of Infectious Diseases</title>
<idno type="ISSN">0022-1899</idno>
<idno type="eISSN">1537-6613</idno>
<imprint><publisher>The University of Chicago Press</publisher>
<date type="published" when="2009-07-15">2009-07-15</date>
<biblScope unit="volume">200</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="216">216</biblScope>
<biblScope unit="page" to="226">226</biblScope>
</imprint>
<idno type="ISSN">0022-1899</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0022-1899</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract">BackgroundLittle is known about the impact of episodic treatment of herpes on human immunodeficiency virus type 1 (HIV-1) MethodsWomen from Ghana and the Central African Republic who had genital ulcers were enrolled in a randomized, double-blind, placebo-controlled trial of acyclovir plus antibacterials and were monitored for 28 days. Ulcer etiologies and detection of lesional HIV-1 RNA were determined by polymerase chain reaction (PCR). Cervicovaginal HIV-1 RNA and herpes simplex virus type 2 (HSV-2) DNA and plasma HIV-1 RNA were quantitated by real-time PCR. Primary analyses included 118 HIV-1–infected women with HSV-2 ulcers (54 of whom were given acyclovir and 64 of whom were given placebo) ResultsAcyclovir had little impact on (1) detection of cervicovaginal HIV-1 RNA (risk ratio [RR], 0.96; 95% confidence interval [CI], 0.8–1.2) at day 7 of treatment, (2) the mean cervicovaginal HIV-1 RNA load (−0.06 log10 copies/mL; 95% CI, −0.4 to 0.3 log10 copies/mL) at day 7 of treatment, or (3) the plasma HIV-1 RNA load (+0.09 log10 copies/mL; 95% CI, −0.1 to 0.3 log10 copies/mL) at day 14 of treatment. At day 7, women receiving acyclovir were less likely to have detectable lesional HIV-1 RNA (RR, 0.70; 95% CI, 0.4–1.2) or cervicovaginal HSV-2 DNA (RR, 0.69; 95% CI, 0.4–1.3), had a lower quantity of HSV-2 DNA (−0.99 log10 copies/mL; 95% CI, −1.8 to −0.2 log10 copies/mL), and were more likely to have a healed ulcer (RR, 1.26; 95% CI, 0.9–1.9) ConclusionEpisodic therapy for herpes reduced the quantity of cervicovaginal HSV-2 DNA and slightly improved ulcer healing, but it did not decrease genital and plasma HIV-1 RNA loads Trial registrationClinicalTrials.gov identifier NCT00158483</div>
</front>
</TEI>
<istex><corpusName>oup</corpusName>
<author><json:item><name>Philippe Mayaud</name>
<affiliations><json:string>Clinical Research Unit, Department of Infectious and Tropical Diseases, and</json:string>
<json:string>Reprints or correspondence: Philippe Mayaud, Clinical Research Unit, London School of Hygiene & Tropical Medicine, Keppel St., London WC1E 7HT, UK (philippe.mayaud@lshtm.ac.uk)</json:string>
</affiliations>
</json:item>
<json:item><name>Jérôme LeGoff</name>
<affiliations><json:string>Université Paris Descartes, Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743 (Immunologie Humaine), Centre de Recherches Biomédicales des Cordeliers and Laboratoire de Virologie, Hôpital Européen Georges Pompidou,</json:string>
<json:string>Université Paris Diderot, Laboratoire de Microbiologie, Hôpital Saint-Louis, and</json:string>
</affiliations>
</json:item>
<json:item><name>Helen A. Weiss</name>
<affiliations><json:string>Infectious Disease Epidemiology Unit, Department of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, London, United Kingdom;</json:string>
</affiliations>
</json:item>
<json:item><name>Gérard Grésenguet</name>
<affiliations><json:string>Centre National de Référence des Maladies Sexuellement Transmissibles et du SIDA de Bangui and Unité de Recherches et d’Intervention sur les Maladies Sexuellement Transmissibles et du SIDA, Faculté des Sciences de la Santé, Bangui, Central African Republic;</json:string>
</affiliations>
</json:item>
<json:item><name>Khonde Nzambi</name>
<affiliations><json:string>West African Project To Combat AIDS and STIs, Accra, Ghana;</json:string>
</affiliations>
</json:item>
<json:item><name>Hicham Bouhlal</name>
<affiliations><json:string>Université Paris Descartes, Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743 (Immunologie Humaine), Centre de Recherches Biomédicales des Cordeliers and Laboratoire de Virologie, Hôpital Européen Georges Pompidou,</json:string>
</affiliations>
</json:item>
<json:item><name>Eric Frost</name>
<affiliations><json:string>Centre for International Health, University of Sherbrooke, Sherbrooke, Canada</json:string>
</affiliations>
</json:item>
<json:item><name>Jacques Pépin</name>
<affiliations><json:string>Centre for International Health, University of Sherbrooke, Sherbrooke, Canada</json:string>
</affiliations>
</json:item>
<json:item><name>Jean-Elie Malkin</name>
<affiliations><json:string>Centre Médical, Institut Pasteur, Paris, France;</json:string>
</affiliations>
</json:item>
<json:item><name>Richard J. Hayes</name>
<affiliations><json:string>Infectious Disease Epidemiology Unit, Department of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, London, United Kingdom;</json:string>
</affiliations>
</json:item>
<json:item><name>David C. W. Mabey</name>
<affiliations><json:string>Clinical Research Unit, Department of Infectious and Tropical Diseases, and</json:string>
</affiliations>
</json:item>
<json:item><name>Laurent Bélec</name>
<affiliations><json:string>Université Paris Descartes, Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743 (Immunologie Humaine), Centre de Recherches Biomédicales des Cordeliers and Laboratoire de Virologie, Hôpital Européen Georges Pompidou,</json:string>
</affiliations>
</json:item>
</author>
<language><json:string>unknown</json:string>
</language>
<originalGenre><json:string>research-article</json:string>
</originalGenre>
<abstract>BackgroundLittle is known about the impact of episodic treatment of herpes on human immunodeficiency virus type 1 (HIV-1) MethodsWomen from Ghana and the Central African Republic who had genital ulcers were enrolled in a randomized, double-blind, placebo-controlled trial of acyclovir plus antibacterials and were monitored for 28 days. Ulcer etiologies and detection of lesional HIV-1 RNA were determined by polymerase chain reaction (PCR). Cervicovaginal HIV-1 RNA and herpes simplex virus type 2 (HSV-2) DNA and plasma HIV-1 RNA were quantitated by real-time PCR. Primary analyses included 118 HIV-1–infected women with HSV-2 ulcers (54 of whom were given acyclovir and 64 of whom were given placebo) ResultsAcyclovir had little impact on (1) detection of cervicovaginal HIV-1 RNA (risk ratio [RR], 0.96; 95% confidence interval [CI], 0.8–1.2) at day 7 of treatment, (2) the mean cervicovaginal HIV-1 RNA load (−0.06 log10 copies/mL; 95% CI, −0.4 to 0.3 log10 copies/mL) at day 7 of treatment, or (3) the plasma HIV-1 RNA load (+0.09 log10 copies/mL; 95% CI, −0.1 to 0.3 log10 copies/mL) at day 14 of treatment. At day 7, women receiving acyclovir were less likely to have detectable lesional HIV-1 RNA (RR, 0.70; 95% CI, 0.4–1.2) or cervicovaginal HSV-2 DNA (RR, 0.69; 95% CI, 0.4–1.3), had a lower quantity of HSV-2 DNA (−0.99 log10 copies/mL; 95% CI, −1.8 to −0.2 log10 copies/mL), and were more likely to have a healed ulcer (RR, 1.26; 95% CI, 0.9–1.9) ConclusionEpisodic therapy for herpes reduced the quantity of cervicovaginal HSV-2 DNA and slightly improved ulcer healing, but it did not decrease genital and plasma HIV-1 RNA loads Trial registrationClinicalTrials.gov identifier NCT00158483</abstract>
<qualityIndicators><score>10</score>
<pdfVersion>1.4</pdfVersion>
<pdfPageSize>612 x 792 pts (letter)</pdfPageSize>
<refBibsNative>true</refBibsNative>
<keywordCount>0</keywordCount>
<abstractCharCount>1702</abstractCharCount>
<pdfWordCount>6921</pdfWordCount>
<pdfCharCount>42409</pdfCharCount>
<pdfPageCount>11</pdfPageCount>
<abstractWordCount>265</abstractWordCount>
</qualityIndicators>
<title>Impact of Acyclovir on Genital and Plasma HIV-1 RNA, Genital Herpes Simplex Virus Type 2 DNA, and Ulcer Healing among HIV-1–Infected African Women with Herpes Ulcers: A Randomized Placebo-Controlled Trial</title>
<corporate><json:item><name>for the ANRS 1212 Study Group</name>
</json:item>
</corporate>
<genre><json:string>research-article</json:string>
</genre>
<host><title>The Journal of Infectious Diseases</title>
<language><json:string>unknown</json:string>
</language>
<issn><json:string>0022-1899</json:string>
</issn>
<eissn><json:string>1537-6613</json:string>
</eissn>
<publisherId><json:string>jid</json:string>
</publisherId>
<volume>200</volume>
<issue>2</issue>
<pages><first>216</first>
<last>226</last>
</pages>
<genre><json:string>journal</json:string>
</genre>
</host>
<categories><wos><json:string>science</json:string>
<json:string>microbiology</json:string>
<json:string>infectious diseases</json:string>
<json:string>immunology</json:string>
</wos>
<scienceMetrix><json:string>health sciences</json:string>
<json:string>biomedical research</json:string>
<json:string>microbiology</json:string>
</scienceMetrix>
<inist><json:string>sciences appliquees, technologies et medecines</json:string>
<json:string>sciences biologiques et medicales</json:string>
<json:string>sciences medicales</json:string>
</inist>
</categories>
<publicationDate>2009</publicationDate>
<copyrightDate>2009</copyrightDate>
<doi><json:string>10.1086/599991</json:string>
</doi>
<id>A8D7F03BC8675B8AACE47022187702C7B7DE22EE</id>
<score>1</score>
<fulltext><json:item><extension>pdf</extension>
<original>true</original>
<mimetype>application/pdf</mimetype>
<uri>https://api.istex.fr/document/A8D7F03BC8675B8AACE47022187702C7B7DE22EE/fulltext/pdf</uri>
</json:item>
<json:item><extension>zip</extension>
<original>false</original>
<mimetype>application/zip</mimetype>
<uri>https://api.istex.fr/document/A8D7F03BC8675B8AACE47022187702C7B7DE22EE/fulltext/zip</uri>
</json:item>
<istex:fulltextTEI uri="https://api.istex.fr/document/A8D7F03BC8675B8AACE47022187702C7B7DE22EE/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Impact of Acyclovir on Genital and Plasma HIV-1 RNA, Genital Herpes Simplex Virus Type 2 DNA, and Ulcer Healing among HIV-1–Infected African Women with Herpes Ulcers: A Randomized Placebo-Controlled Trial</title>
</titleStmt>
<publicationStmt><authority>ISTEX</authority>
<publisher scheme="https://publisher-list.data.istex.fr">The University of Chicago Press</publisher>
<availability><licence><p>© 2009 by the Infectious Diseases Society of America</p>
</licence>
<p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-GTWS0RDP-M">oup</p>
</availability>
<date>2009</date>
</publicationStmt>
<notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note>
<note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note>
</notesStmt>
<sourceDesc><biblStruct type="inbook"><analytic><title level="a">Impact of Acyclovir on Genital and Plasma HIV-1 RNA, Genital Herpes Simplex Virus Type 2 DNA, and Ulcer Healing among HIV-1–Infected African Women with Herpes Ulcers: A Randomized Placebo-Controlled Trial</title>
<author role="org"><orgName>for the ANRS 1212 Study Group</orgName>
<affiliation>Reprints or correspondence: Philippe Mayaud, Clinical Research Unit, London School of Hygiene & Tropical Medicine, Keppel St., London WC1E 7HT, UK (philippe.mayaud@lshtm.ac.uk)</affiliation>
</author>
<author xml:id="author-0000"><persName><forename type="first">Philippe</forename>
<surname>Mayaud</surname>
</persName>
<affiliation>Clinical Research Unit, Department of Infectious and Tropical Diseases, and</affiliation>
<affiliation>Reprints or correspondence: Philippe Mayaud, Clinical Research Unit, London School of Hygiene & Tropical Medicine, Keppel St., London WC1E 7HT, UK (philippe.mayaud@lshtm.ac.uk)</affiliation>
</author>
<author xml:id="author-0001"><persName><forename type="first">Jérôme</forename>
<surname>LeGoff</surname>
</persName>
<affiliation>Université Paris Descartes, Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743 (Immunologie Humaine), Centre de Recherches Biomédicales des Cordeliers and Laboratoire de Virologie, Hôpital Européen Georges Pompidou,</affiliation>
<affiliation>Université Paris Diderot, Laboratoire de Microbiologie, Hôpital Saint-Louis, and</affiliation>
</author>
<author xml:id="author-0002"><persName><forename type="first">Helen A.</forename>
<surname>Weiss</surname>
</persName>
<affiliation>Infectious Disease Epidemiology Unit, Department of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, London, United Kingdom;</affiliation>
</author>
<author xml:id="author-0003"><persName><forename type="first">Gérard</forename>
<surname>Grésenguet</surname>
</persName>
<affiliation>Centre National de Référence des Maladies Sexuellement Transmissibles et du SIDA de Bangui and Unité de Recherches et d’Intervention sur les Maladies Sexuellement Transmissibles et du SIDA, Faculté des Sciences de la Santé, Bangui, Central African Republic;</affiliation>
</author>
<author xml:id="author-0004"><persName><forename type="first">Khonde</forename>
<surname>Nzambi</surname>
</persName>
<affiliation>West African Project To Combat AIDS and STIs, Accra, Ghana;</affiliation>
</author>
<author xml:id="author-0005"><persName><forename type="first">Hicham</forename>
<surname>Bouhlal</surname>
</persName>
<affiliation>Université Paris Descartes, Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743 (Immunologie Humaine), Centre de Recherches Biomédicales des Cordeliers and Laboratoire de Virologie, Hôpital Européen Georges Pompidou,</affiliation>
</author>
<author xml:id="author-0006"><persName><forename type="first">Eric</forename>
<surname>Frost</surname>
</persName>
<affiliation>Centre for International Health, University of Sherbrooke, Sherbrooke, Canada</affiliation>
</author>
<author xml:id="author-0007"><persName><forename type="first">Jacques</forename>
<surname>Pépin</surname>
</persName>
<affiliation>Centre for International Health, University of Sherbrooke, Sherbrooke, Canada</affiliation>
</author>
<author xml:id="author-0008"><persName><forename type="first">Jean-Elie</forename>
<surname>Malkin</surname>
</persName>
<affiliation>Centre Médical, Institut Pasteur, Paris, France;</affiliation>
</author>
<author xml:id="author-0009"><persName><forename type="first">Richard J.</forename>
<surname>Hayes</surname>
</persName>
<affiliation>Infectious Disease Epidemiology Unit, Department of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, London, United Kingdom;</affiliation>
</author>
<author xml:id="author-0010"><persName><forename type="first">David C. W.</forename>
<surname>Mabey</surname>
</persName>
<affiliation>Clinical Research Unit, Department of Infectious and Tropical Diseases, and</affiliation>
</author>
<author xml:id="author-0011"><persName><forename type="first">Laurent</forename>
<surname>Bélec</surname>
</persName>
<affiliation>Université Paris Descartes, Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743 (Immunologie Humaine), Centre de Recherches Biomédicales des Cordeliers and Laboratoire de Virologie, Hôpital Européen Georges Pompidou,</affiliation>
</author>
<idno type="istex">A8D7F03BC8675B8AACE47022187702C7B7DE22EE</idno>
<idno type="ark">ark:/67375/HXZ-KC5GD94Z-2</idno>
<idno type="DOI">10.1086/599991</idno>
</analytic>
<monogr><title level="j">The Journal of Infectious Diseases</title>
<title level="j" type="abbrev">The Journal of Infectious Diseases</title>
<idno type="pISSN">0022-1899</idno>
<idno type="eISSN">1537-6613</idno>
<idno type="publisher-id">jid</idno>
<idno type="PublisherID-hwp">jinfdis</idno>
<imprint><publisher>The University of Chicago Press</publisher>
<date type="published" when="2009-07-15"></date>
<biblScope unit="volume">200</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="216">216</biblScope>
<biblScope unit="page" to="226">226</biblScope>
</imprint>
</monogr>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><creation><date>2009</date>
</creation>
<abstract><p>BackgroundLittle is known about the impact of episodic treatment of herpes on human immunodeficiency virus type 1 (HIV-1) MethodsWomen from Ghana and the Central African Republic who had genital ulcers were enrolled in a randomized, double-blind, placebo-controlled trial of acyclovir plus antibacterials and were monitored for 28 days. Ulcer etiologies and detection of lesional HIV-1 RNA were determined by polymerase chain reaction (PCR). Cervicovaginal HIV-1 RNA and herpes simplex virus type 2 (HSV-2) DNA and plasma HIV-1 RNA were quantitated by real-time PCR. Primary analyses included 118 HIV-1–infected women with HSV-2 ulcers (54 of whom were given acyclovir and 64 of whom were given placebo) ResultsAcyclovir had little impact on (1) detection of cervicovaginal HIV-1 RNA (risk ratio [RR], 0.96; 95% confidence interval [CI], 0.8–1.2) at day 7 of treatment, (2) the mean cervicovaginal HIV-1 RNA load (−0.06 log10 copies/mL; 95% CI, −0.4 to 0.3 log10 copies/mL) at day 7 of treatment, or (3) the plasma HIV-1 RNA load (+0.09 log10 copies/mL; 95% CI, −0.1 to 0.3 log10 copies/mL) at day 14 of treatment. At day 7, women receiving acyclovir were less likely to have detectable lesional HIV-1 RNA (RR, 0.70; 95% CI, 0.4–1.2) or cervicovaginal HSV-2 DNA (RR, 0.69; 95% CI, 0.4–1.3), had a lower quantity of HSV-2 DNA (−0.99 log10 copies/mL; 95% CI, −1.8 to −0.2 log10 copies/mL), and were more likely to have a healed ulcer (RR, 1.26; 95% CI, 0.9–1.9) ConclusionEpisodic therapy for herpes reduced the quantity of cervicovaginal HSV-2 DNA and slightly improved ulcer healing, but it did not decrease genital and plasma HIV-1 RNA loads Trial registrationClinicalTrials.gov identifier NCT00158483</p>
</abstract>
</profileDesc>
<revisionDesc><change when="2009-07-15">Published</change>
</revisionDesc>
</teiHeader>
</istex:fulltextTEI>
<json:item><extension>txt</extension>
<original>false</original>
<mimetype>text/plain</mimetype>
<uri>https://api.istex.fr/document/A8D7F03BC8675B8AACE47022187702C7B7DE22EE/fulltext/txt</uri>
</json:item>
</fulltext>
<metadata><istex:metadataXml wicri:clean="corpus oup, element #text not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0"</istex:xmlDeclaration>
<istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v2.3 20070202//EN" URI="journalpublishing.dtd" name="istex:docType"></istex:docType>
<istex:document><article article-type="research-article">
<front>
<journal-meta>
<journal-id journal-id-type="hwp">jinfdis</journal-id>
<journal-id journal-id-type="publisher-id">jid</journal-id>
<journal-title>The Journal of Infectious Diseases</journal-title>
<abbrev-journal-title>The Journal of Infectious Diseases</abbrev-journal-title>
<issn pub-type="ppub">0022-1899</issn>
<issn pub-type="epub">1537-6613</issn>
<publisher>
<publisher-name>The University of Chicago Press</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="doi">10.1086/599991</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Major Articles and Brief Reports</subject>
<subj-group subj-group-type="heading">
<subject>HIV/AIDS</subject>
<subj-group subj-group-type="heading">
<subject>Major Articles</subject>
</subj-group>
</subj-group>
</subj-group>
</article-categories>
<title-group>
<article-title>Impact of Acyclovir on Genital and Plasma HIV-1 RNA, Genital Herpes Simplex Virus Type 2 DNA, and Ulcer Healing among HIV-1–Infected African Women with Herpes Ulcers: A Randomized Placebo-Controlled Trial<xref ref-type="fn" rid="fn1"></xref>
</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author" corresp="yes">
<name><surname>Mayaud</surname>
<given-names>Philippe</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author">
<name><surname>LeGoff</surname>
<given-names>Jérôme</given-names>
</name>
<xref ref-type="aff" rid="aff3">3</xref>
<xref ref-type="aff" rid="aff4">4</xref>
</contrib>
<contrib contrib-type="author">
<name><surname>Weiss</surname>
<given-names>Helen A.</given-names>
</name>
<xref ref-type="aff" rid="aff2">2</xref>
</contrib>
<contrib contrib-type="author">
<name><surname>Grésenguet</surname>
<given-names>Gérard</given-names>
</name>
<xref ref-type="aff" rid="aff6">6</xref>
</contrib>
<contrib contrib-type="author">
<name><surname>Nzambi</surname>
<given-names>Khonde</given-names>
</name>
<xref ref-type="aff" rid="aff7">7</xref>
</contrib>
<contrib contrib-type="author">
<name><surname>Bouhlal</surname>
<given-names>Hicham</given-names>
</name>
<xref ref-type="aff" rid="aff3">3</xref>
</contrib>
<contrib contrib-type="author">
<name><surname>Frost</surname>
<given-names>Eric</given-names>
</name>
<xref ref-type="aff" rid="aff8">8</xref>
</contrib>
<contrib contrib-type="author">
<name><surname>Pépin</surname>
<given-names>Jacques</given-names>
</name>
<xref ref-type="aff" rid="aff8">8</xref>
</contrib>
<contrib contrib-type="author">
<name><surname>Malkin</surname>
<given-names>Jean-Elie</given-names>
</name>
<xref ref-type="aff" rid="aff5">5</xref>
</contrib>
<contrib contrib-type="author">
<name><surname>Hayes</surname>
<given-names>Richard J.</given-names>
</name>
<xref ref-type="aff" rid="aff2">2</xref>
</contrib>
<contrib contrib-type="author">
<name><surname>Mabey</surname>
<given-names>David C. W.</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author">
<name><surname>Bélec</surname>
<given-names>Laurent</given-names>
</name>
<xref ref-type="aff" rid="aff3">3</xref>
</contrib>
<contrib contrib-type="author">
<collab>for the ANRS 1212 Study Group</collab>
<xref ref-type="fn" rid="fn2">a</xref>
<xref ref-type="fn" rid="fn3"></xref>
</contrib>
<aff id="aff1">
<label>1</label>
Clinical Research Unit, Department of Infectious and Tropical Diseases, and</aff>
<aff id="aff2">
<label>2</label>
Infectious Disease Epidemiology Unit, Department of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, London, United Kingdom; </aff>
<aff id="aff3">
<label>3</label>
Université Paris Descartes, Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743 (Immunologie Humaine), Centre de Recherches Biomédicales des Cordeliers and Laboratoire de Virologie, Hôpital Européen Georges Pompidou, </aff>
<aff id="aff4">
<label>4</label>
Université Paris Diderot, Laboratoire de Microbiologie, Hôpital Saint-Louis, and</aff>
<aff id="aff5">
<label>5</label>
Centre Médical, Institut Pasteur, Paris, France; </aff>
<aff id="aff6">
<label>6</label>
Centre National de Référence des Maladies Sexuellement Transmissibles et du SIDA de Bangui and Unité de Recherches et d’Intervention sur les Maladies Sexuellement Transmissibles et du SIDA, Faculté des Sciences de la Santé, Bangui, Central African Republic; </aff>
<aff id="aff7">
<label>7</label>
West African Project To Combat AIDS and STIs, Accra, Ghana; </aff>
<aff id="aff8">
<label>8</label>
Centre for International Health, University of Sherbrooke, Sherbrooke, Canada</aff>
</contrib-group>
<author-notes>
<corresp id="cor1">Reprints or correspondence: Philippe Mayaud, Clinical Research Unit, London School of Hygiene & Tropical Medicine, Keppel St., London WC1E 7HT, UK (<email>philippe.mayaud@lshtm.ac.uk</email>
)</corresp>
</author-notes>
<pub-date pub-type="ppub">
<day>15</day>
<month>7</month>
<year>2009</year>
</pub-date>
<volume>200</volume>
<issue>2</issue>
<fpage>216</fpage>
<lpage>226</lpage>
<history>
<date date-type="received">
<day>17</day>
<month>12</month>
<year>2008</year>
</date>
<date date-type="accepted">
<day>25</day>
<month>2</month>
<year>2009</year>
</date>
</history>
<copyright-statement>© 2009 by the Infectious Diseases Society of America</copyright-statement>
<copyright-year>2009</copyright-year>
<abstract>
<p><bold>Background</bold>
Little is known about the impact of episodic treatment of herpes on human immunodeficiency virus type 1 (HIV-1)</p>
<p><bold>Methods</bold>
Women from Ghana and the Central African Republic who had genital ulcers were enrolled in a randomized, double-blind, placebo-controlled trial of acyclovir plus antibacterials and were monitored for 28 days. Ulcer etiologies and detection of lesional HIV-1 RNA were determined by polymerase chain reaction (PCR). Cervicovaginal HIV-1 RNA and herpes simplex virus type 2 (HSV-2) DNA and plasma HIV-1 RNA were quantitated by real-time PCR. Primary analyses included 118 HIV-1–infected women with HSV-2 ulcers (54 of whom were given acyclovir and 64 of whom were given placebo)</p>
<p><bold>Results</bold>
Acyclovir had little impact on (1) detection of cervicovaginal HIV-1 RNA (risk ratio [RR], 0.96; 95% confidence interval [CI], 0.8–1.2) at day 7 of treatment, (2) the mean cervicovaginal HIV-1 RNA load (−0.06 log<sub>10</sub>
copies/mL; 95% CI, −0.4 to 0.3 log<sub>10</sub>
copies/mL) at day 7 of treatment, or (3) the plasma HIV-1 RNA load (+0.09 log<sub>10</sub>
copies/mL; 95% CI, −0.1 to 0.3 log<sub>10</sub>
copies/mL) at day 14 of treatment. At day 7, women receiving acyclovir were less likely to have detectable lesional HIV-1 RNA (RR, 0.70; 95% CI, 0.4–1.2) or cervicovaginal HSV-2 DNA (RR, 0.69; 95% CI, 0.4–1.3), had a lower quantity of HSV-2 DNA (−0.99 log<sub>10</sub>
copies/mL; 95% CI, −1.8 to −0.2 log<sub>10</sub>
copies/mL), and were more likely to have a healed ulcer (RR, 1.26; 95% CI, 0.9–1.9)</p>
<p><bold>Conclusion</bold>
Episodic therapy for herpes reduced the quantity of cervicovaginal HSV-2 DNA and slightly improved ulcer healing, but it did not decrease genital and plasma HIV-1 RNA loads</p>
<p><bold>Trial registration</bold>
ClinicalTrials.gov identifier <ext-link ext-link-type="uri" xlink:href="http://ClinicalTrials.gov">NCT00158483</ext-link>
</p>
</abstract>
</article-meta>
</front>
<body>
<p>Herpes simplex virus (HSV) type 2 (HSV-2), the most common cause of genital ulcer disease worldwide [<xref ref-type="bibr" rid="ref1">1</xref>
<xref ref-type="bibr" rid="ref2"></xref>
–<xref ref-type="bibr" rid="ref3">3</xref>
], is associated with the prevalence and incidence of human immunodeficiency virus (HIV) [<xref ref-type="bibr" rid="ref4">4</xref>
, <xref ref-type="bibr" rid="ref5">5</xref>
]. Because of the high prevalence of HSV-2 in many parts of sub-Saharan Africa, a large proportion of HIV-infected individuals will be coinfected [<xref ref-type="bibr" rid="ref1">1</xref>
]. Several studies have demonstrated increased genital shedding of HIV-1 during symptomatic [<xref ref-type="bibr" rid="ref6">6</xref>
, <xref ref-type="bibr" rid="ref7">7</xref>
] and asymptomatic [<xref ref-type="bibr" rid="ref8">8</xref>
<xref ref-type="bibr" rid="ref9"></xref>
–<xref ref-type="bibr" rid="ref10">10</xref>
] recurrences of herpetic episodes, suggesting that HSV-2 may increase replication of HIV type 1 (HIV-1), as has been supported by biological evidence [<xref ref-type="bibr" rid="ref11">11</xref>
]. Recent randomized trials have shown that HSV suppression decreases the detection and quantity of genital and plasma HIV-1 RNA in coinfected individuals [<xref ref-type="bibr" rid="ref12">12</xref>
<xref ref-type="bibr" rid="ref13"></xref>
<xref ref-type="bibr" rid="ref14"></xref>
<xref ref-type="bibr" rid="ref15"></xref>
–<xref ref-type="bibr" rid="ref16">16</xref>
]. Additional data on a possible causal role of HSV-2 in HIV transmission will emerge from an ongoing trial of the impact among HIV-serodiscordant couples of HSV suppressive therapy given to the HIV-infected partner [<xref ref-type="bibr" rid="ref17">17</xref>
]. Little is known, however, about the impact on HIV-1 of episodic antiherpetic therapy administered during symptomatic ulcerations</p>
<p>HIV infection alters the natural history of HSV-2 infection, and severely immunosuppressed coinfected patients may experience more frequent, severe, or prolonged symptomatic recurrences [<xref ref-type="bibr" rid="ref18">18</xref>
, <xref ref-type="bibr" rid="ref19">19</xref>
]. Moreover, observational studies have shown transient increases in the plasma HIV load during symptomatic episodes of genital herpes [<xref ref-type="bibr" rid="ref20">20</xref>
]; these increases can be reduced by the use of antiherpetic therapy [<xref ref-type="bibr" rid="ref21">21</xref>
]. Among HIV-serodiscordant couples in Uganda, incident genital ulcer disease (mostly caused by HSV-2) in the HIV-infected partner was associated with a 4-fold increase in HIV transmission [<xref ref-type="bibr" rid="ref22">22</xref>
]. Thus, herpetic ulcers may contribute substantially to HIV transmission, and HSV treatment may reduce transmissibility</p>
<p>Management guidelines from the World Health Organization (WHO) recommend antiherpetic therapy for patients with genital ulcer disease for whom the proportion of ulcers caused by HSV-2 is ⩾30% [<xref ref-type="bibr" rid="ref23">23</xref>
]. However, herpes therapy is seldom used in resource-poor settings, and the impact of incorporating acyclovir into syndromic management has not been evaluated [<xref ref-type="bibr" rid="ref3">3</xref>
]. We present the results of what is, to our knowledge, the first trial of episodic antiherpetic therapy that used acyclovir in addition to standard syndromic genital ulcer disease treatment in Africa. The primary aim of the study was to assess the impact of this intervention on the detection and quantity of cervicovaginal HIV-1 RNA among HIV-1–infected women with herpetic ulcers. The secondary aims of the study were to evaluate the impact of the intervention on the detection of lesional HIV-1 RNA, the quantity of plasma HIV-1 RNA, the detection and quantity of cervicovaginal HSV-2 DNA, and the proportion of healed ulcers</p>
<sec id="S1">
<title>Methods</title>
<p>The Agence Nationale de Recherches sur le SIDA et les Hépatites (ANRS) 1212 trial was an individually randomized placebo-controlled trial of 400 mg of acyclovir given 3 times daily for 5 days, in accordance with standard recommendations [<xref ref-type="bibr" rid="ref23">23</xref>
<xref ref-type="bibr" rid="ref24"></xref>
–<xref ref-type="bibr" rid="ref25">25</xref>
], plus antibacterials for the syndromic management of genital ulcer disease. The study was approved by the research ethics committees of the Ministries of Health of Ghana and the Central African Republic and the London School of Hygiene & Tropical Medicine</p>
<p>Women with clinically confirmed ulcers or blisters in Accra and Kumasi (Ghana) and in Bangui (Central African Republic) were eligible for recruitment into the study if they were ⩾18 years of age and resided and planned to stay in Accra, Kumasi, or Bangui for 1 month. Exclusion criteria included menstruation; pregnancy (as determined by urine testing with Rapid HCG [Dectra Pharm]); contraindications to acyclovir (e.g., suspected renal impairment or allergy to acyclovir); and the presence of large ulcers (surface area, ⩾500 mm<sup>2</sup>
), chronic ulcers (duration, >1 month), or perianal ulcers only, which prompted provision of open-label acyclovir. All women were offered voluntary HIV counseling. HIV serostatus was determined after consent was provided. Women who elected to know their HIV serostatus and who were eligible for highly active antiretroviral therapy (HAART) were referred to appropriate health care providers</p>
<p>Women who provided consent were interviewed and examined and had genital and blood samples collected. Study participants received directly observed single-dose syndromic treatment for chancroid (500 mg of ciprofloxacin given orally) and syphilis (2.4 million U of benzathine penicillin given intramuscularly), counseling about sexually transmitted infections and HIV, partner notification slips, and condoms. Participants were asked to return to the clinic on day 2 and/or day 4 (depending on the day of study enrollment) and on days 7, 14, and 28 (hereafter known as “D7,” “D14,” and “D28,” respectively). At each clinic visit, a pelvic examination was performed and genital samples collected. Genital sampling was deferred for menstruating women until 2 days after bleeding had ceased. During treatment, physicians assessed treatment adherence by pill counts, recorded and graded side effects and adverse events, and encouraged compliance. Participants whose ulcers had not improved by D14 of treatment (as defined by a <90% reduction in the surface area of the ulcer at D14, compared with that noted on day 0 [D0]) were given open-label acyclovir for 5 days</p>
<p>Participants were randomly assigned to receive acyclovir or placebo according to a 1:1 allocation scheme using block randomization (size, 10), in accordance with a random allocation list provided by the study drug manufacturer (Creapharm). Investigators and participants were blinded to treatment allocation through the use of prelabeled sequentially numbered treatment packs</p>
<p><bold><italic>Specimen collection and laboratory procedures</italic>
</bold>
Details about the sampling and testing procedures used have been published elsewhere [<xref ref-type="bibr" rid="ref7">7</xref>
]. In brief, at the enrollment clinic visit (D0) and at each follow-up visit, clinical characteristics were recorded, and the largest ulcer was measured using a disposable ruler. The surface area of the ulcer was estimated by multiplying its length by its width. For all participants, at D0 and at follow-up clinic visits, if the ulcer had not improved (if there was <75% reduction in the surface area of the largest ulcer at D7, compared with D0, and if there was <90% reduction in the surface area of the largest ulcer at D14, compared with D0), 2 dry Dacron swabs were collected from the base of the ulcer to determine ulcer etiology and test for lesional HIV-1 RNA (among HIV-positive patients). Lesional swab specimens were tested for HSV, <italic>Haemophilus ducreyi, Treponema pallidum, Klebsiella granulomatis</italic>
and <italic>Chlamydia trachomatis</italic>
lymphogranuloma venereum by use of molecular techniques [<xref ref-type="bibr" rid="ref7">7</xref>
]</p>
<p>At each clinic visit, a standardized 60-s cervicovaginal lavage specimen with 3 mL of phosphate-buffered saline fluid was collected, immediately processed, and stored [<xref ref-type="bibr" rid="ref26">26</xref>
]. Vaginal and cervical swab specimens were collected at enrollment (D0) for the diagnosis of vaginal and cervical infections [<xref ref-type="bibr" rid="ref7">7</xref>
]</p>
<p>At D0, blood samples were collected for the serologic diagnosis of HIV-1 (by use of 2 complementary enzyme-linked immunosorbent assays [ELISAs]), HSV (by use of specific immunoglobulin [Ig] G1 and IgG2 ELISAs for HSV-1 and HSV-2 [HerpeSelect; Focus Technologies] at the manufacturer’s recommended threshold of 1.1), and syphilis (by use of a rapid plasma reagin test, the results of which were confirmed by <italic>T. pallidum</italic>
hemagglutination assay) [<xref ref-type="bibr" rid="ref7">7</xref>
, <xref ref-type="bibr" rid="ref27">27</xref>
]. Serologic tests were performed again at D28 for those women who were initially found to be seronegative. In addition, among HIV-seropositive women, the CD4<sup>+</sup>
cell count was measured by FACScount (Becton Dickinson) at enrollment, and plasma HIV-1 RNA levels were measured on D0, D14, and D28</p>
<p>Detection and quantitation of HIV-1 RNA and HSV-2 DNA were performed in the acellular fraction of the cervicovaginal lavage specimen, and detection and quantitation of HIV-1 RNA in plasma were determined by real-time polymerase chain reaction (PCR) (detection threshold, 250 copies/mL) [<xref ref-type="bibr" rid="ref28">28</xref>
]. Lesional HIV-1 RNA was detected qualitatively by real-time PCR. To avoid possible misclassification of samples due to the presence of semen in the vagina, cervicovaginal lavage specimens were tested for the presence of the Y chromosome by means of PCR [<xref ref-type="bibr" rid="ref7">7</xref>
]</p>
<p><bold><italic>Study definitions</italic>
</bold>
We defined HIV-1 “infection” as detection of HIV-1 either in a cervicovaginal lavage specimen or serologically. A nonprimary first episode of genital HSV-2 infection was defined as detection of HSV-2 DNA in the lesion of or cervicovaginal lavage specimen obtained from women who were HSV-1 seropositive and HSV-2 seronegative. We used 2 definitions of ulcer healing at each clinic visit: (1) a ⩾90% reduction in the surface area of the largest ulcer, compared with its size at D0, and (2) the proportion of patients whose largest ulcer was ⩽10 mm<sup>2</sup>
. Fully reepithelialized ulcers that were not measured were considered to be “healed” ulcers, according to both definitions</p>
<p><bold><italic>Statistical considerations</italic>
</bold>
Sample-size calculations were based on a previous study performed in Bangui [<xref ref-type="bibr" rid="ref8">8</xref>
]. Assuming 60% prevalence of cervicovaginal HIV-1 RNA in the placebo group, we estimated that enrollment of 450 women with genital ulcers would provide 90% power to detect a halving of detectable cervicovaginal HIV-1 RNA at D7 at the 95% significance level, under the assumption that 50% of women were HIV-1 infected and that 60% of these women had herpetic ulcers and with allowance for a 10% loss to follow-up. Under these assumptions, we aimed to enroll 135 HIV-1–infected women with lesional HSV-2 DNA</p>
<p>Women were enrolled and randomized irrespective of their HIV/HSV status, because this status was unknown at D0. However, the a priori–defined primary analysis group included only HIV-1–infected women with lesional HSV-2 DNA. The primary end point was D7 (i.e., the D7 clinic visit [visits on days 6–8 were included in this analysis]). Primary outcomes were the detection and quantitation of cervicovaginal HIV-1 RNA at D7. Secondary outcomes included the detection of lesional HIV-1 RNA at D7; quantitation of plasma HIV-1 RNA at D14 (visits on days 13–15 were included in this analysis) and D28 (visits on days 26–30 were included in this analysis); detection and quantitation of cervicovaginal HSV-2 DNA; and the proportion of ulcers healed at D7</p>
<p>Intention-to-treat analyses were conducted using Stata software (version 9.0; StataCorp). Risk ratios (RRs) and 95% confidence intervals (CIs) were estimated by Poisson regression with robust standard errors [<xref ref-type="bibr" rid="ref29">29</xref>
], adjusting for detection of the outcome measurement at D0. The effects on viral loads among women with detectable virus were assessed by linear regression analysis, adjusting for mean viral loads at D0. Viral loads below the threshold of detection were assigned a value that was one-half the value of the threshold. We used generalized estimating equations (logistic or linear regression) to analyze data from all follow-up visits, adjusting for measurements at baseline. Cox regression was used to analyze the effect on rates of ulcer healing, with Kaplan-Meier survival curves showing the time to healing. All <italic>P</italic>
values were 2-sided</p>
</sec>
<sec id="S2">
<title>Results</title>
<p>Of 490 women who were assessed for study enrollment eligibility from May 2003 through October 2005, a total of 441 (90%) were enrolled and randomized to receive acyclovir (n=221) or placebo (n=220) (<xref ref-type="fig" rid="Fig1">figure 1</xref>
). Reported adherence was very high, with 99% of returning women in each group having taken all their pills by D7. Acyclovir was well tolerated, with no serious adverse events reported. Overall, 203 women (46%) were HIV-1 seropositive. Of the 3 HIV-seronegative women who had detectable cervicovaginal HIV-1 RNA (with no semen detected by Y chromosome PCR), 2 seroconverted at a subsequent visit (primary HIV infection). Of the 206 HIV-infected women, 118 (57%) had HSV-2 ulcers and were included in the primary analysis; 54 of these patients were treated with acyclovir, and 64 were given placebo (<xref ref-type="fig" rid="Fig1">figure 1</xref>
). Of these 118 women, 5 (4%) were HSV-2 seronegative and HSV-1 seropositive and were categorized as having a nonprimary first episode of genital HSV-2 infection</p>
<p>Of the 118 HIV-1–infected women with HSV-2 ulcers, 12 (10%) were lost to follow-up by the clinic visit at D7, and an additional 17 women (14%) did not have a cervicovaginal lavage specimen analyzed at D7, usually because of menstruation. Therefore, 89 women (75%) (41 of whom were treated with acyclovir and 48 of whom were given placebo) were analyzed to determine the impact of acyclovir at the primary end point (<xref ref-type="fig" rid="Fig1">figure 1</xref>
). None of the women with primary HIV infection or a first episode of genital HSV-2 infection had a cervicovaginal lavage specimen analyzed at D7. Overall, 170 (79%) of 216 and 218 (85%) of 256 scheduled follow-up visits were completed in the acyclovir and placebo groups, respectively; 93 (79%) of 118 women were seen at D14, and 77 (65%) of 118 women were seen at D28</p>
<p>Demographic, behavioral, and clinical characteristics were generally similar between treatment groups (<xref ref-type="fig" rid="tb1">table 1</xref>
). The mean age of the study participants was 31 years (range, 18–57 years). Almost one-half of the participants (52 [44%] of 118 women) reported having experienced genital ulcers in the year preceding their current episode, and most women (71 [60%] of 118 women) were currently experiencing ⩾2 ulcers, the largest having an average size of 120 mm<sup>2</sup>
(interquartile range [IQR], 50–198 mm<sup>2</sup>
). The median duration of an ulcer before enrollment was 7 days (IQR, 4–15 days) in both groups. Women were generally immunosuppressed (median CD4 cell count, 190 cells/mm<sup>3</sup>
; IQR, 84–520 cells/mm<sup>3</sup>
), although a few (11 [9%] of 118 women) were receiving HAART. Women in the acyclovir group were more likely to have detectable plasma, lesional, and cervicovaginal HIV-1 RNA at baseline</p>
<p><bold><italic>Impact on cervicovaginal, lesional, and plasma HIV-1 RNA</italic>
</bold>
The proportion of women in the acyclovir group who had detectable cervicovaginal HIV-1 RNA decreased from 80% at D0 to 71% at D7, but there was no evidence of an impact of acyclovir on detectable cervicovaginal HIV-1 RNA, after adjusting for detection at D0 (RR, 0.96; 95% CI, 0.8–1.2) (<xref ref-type="fig" rid="tb2">table 2</xref>
). There also was little reduction in the mean quantity of cervicovaginal HIV-1 RNA among women with detectable cervicovaginal HIV-1 RNA at D7 (−0.06 log<sub>10</sub>
copies/mL; 95% CI, −0.4 to 0.3 log<sub>10</sub>
copies/mL). Similarly, the repeated-measures (i.e., per-visit) analyses found little impact of acyclovir on either the detection or quantitation of cervicovaginal HIV-1 RNA (<xref ref-type="fig" rid="tb2">table 2</xref>
and <xref ref-type="fig" rid="Fig2">figure 2</xref>
)</p>
<p><xref ref-type="fig" rid="tb2">Table 2</xref>
also shows the impact of acyclovir on lesional HIV-1 RNA at D7 and on plasma HIV-1 RNA at D14. The proportion of women with detectable lesional HIV-1 RNA decreased in both groups, from 70% to 35% in the placebo group and from 76% to 27% in the acyclovir group (RR, 0.70; 95% CI, 0.4–1.2). There was no effect of acyclovir on the quantity of plasma HIV-1 RNA at D14 (+0.09 log<sub>10</sub>
copies/mL; 95% CI, −0.1 to 0.3 log<sub>10</sub>
copies/mL) or at D28 (0.01 log<sub>10</sub>
copies/mL; 95% CI, −0.3 to 0.4 log<sub>10</sub>
copies/mL) (data not shown)</p>
<p><bold><italic>Impact of acyclovir on cervicovaginal HSV-2 DNA and ulcer healing</italic>
</bold>
Women receiving acyclovir were less likely to have detectable cervicovaginal HSV-2 at D7 than were women receiving placebo (RR, 0.69; 95% CI, 0.4–1.3) (<xref ref-type="fig" rid="tb2">table 2</xref>
), and among those with detectable virus at D7, the quantity of virus was 1 log lower (−0.99 log<sub>10</sub>
copies/mL; 95% CI, −1.8 to −0.2 log<sub>10</sub>
copies/mL). There was also some impact of acyclovir on the detection of cervicovaginal HSV-2 DNA at day 2 or day 4 (RR, 0.73; 95% CI, 0.5–1.1) (data not shown). Differences were greater on the repeated-measures analyses (odds ratio [OR], 0.49 [95% CI, 0.3–0.9]; mean, −0.73 log<sub>10</sub>
copies/mL [95% CI, −1.1 to −0.4 log<sub>10</sub>
copies/mL]) (<xref ref-type="fig" rid="tb2">table 2</xref>
and <xref ref-type="fig" rid="Fig2">figure 2</xref>
)</p>
<p>There was some evidence that acyclovir improved ulcer healing, with 26 (55%) 47 ulcers healed at D7 in the acyclovir group compared with 26 (44%) of 59 ulcers healed in the placebo group (RR, 1.26; 95% CI, 0.9–1.9) and with similar data noted for those with small ulcers (27 [57%] of 47 vs. 25 [42%] of 59 ulcers, respectively; RR, 1.60 [95% CI, 1.0–2.5]) (<xref ref-type="fig" rid="tb2">table 2</xref>
). Cox regression showed a weak effect of acyclovir on the rate of ulcer healing, which was defined as a ⩾90% reduction in ulcer size (hazard ratio [HR], 1.28; 95% CI, 0.9–1.9) (P=.21) (median time to healing, 7 vs. 8 days) (<xref ref-type="fig" rid="Fig3">figure 3</xref>
). Similar results were seen when ulcer healing was defined as a reduction in ulcer size to ⩽10 mm<sup>2</sup>
(HR, 1.35; 95% CI, 0.9–2.0) (P=.14) (median time to healing, 7 vs. 8 days)</p>
<p>At D7, according to ulcer duration before presentation (<7 days vs. ⩾7 days), there was no modification of the effect of acyclovir on genital HIV-1 RNA (P=.58) or ulcer healing (P=.88). However, acyclovir reduced genital HSV-2 DNA among those who presented late (RR, 0.38; 95% CI, 0.2–0.9) but not among those who presented within a week from the onset on an ulcer (RR, 1.55; 95% CI, 0.6–4.2) (P=.03)</p>
</sec>
<sec id="S3">
<title>Discussion</title>
<p>We report what is, to our knowledge, the first randomized trial evaluating the impact of episodic antiviral therapy for HSV-2 added to syndromic management on HIV-1 outcomes among African women presenting with genital ulcer disease. In HIV-1–infected women with HSV-2 ulcers, there was no effect of acyclovir on cervicovaginal and plasma HIV-1, some evidence of reduction in lesional shedding of HIV-1 and of reduced frequency and quantity of cervicovaginal shedding of HSV-2, and only modest improvements in ulcer healing</p>
<p>The rationale for this study was multifold. First, epidemiologic and biological evidence has suggested an important role of HSV in enhancing HIV replication and, possibly, transmission. Clinical and subclinical reactivation of genital HSV-2 has been associated with increases in HIV-1 genital shedding [<xref ref-type="bibr" rid="ref6">6</xref>
<xref ref-type="bibr" rid="ref7"></xref>
<xref ref-type="bibr" rid="ref8"></xref>
<xref ref-type="bibr" rid="ref9"></xref>
–<xref ref-type="bibr" rid="ref10">10</xref>
, <xref ref-type="bibr" rid="ref30">30</xref>
] and plasma HIV-1 loads [<xref ref-type="bibr" rid="ref10">10</xref>
, <xref ref-type="bibr" rid="ref20">20</xref>
, <xref ref-type="bibr" rid="ref21">21</xref>
], and observational studies of HSV episodic therapy have shown that acyclovir has a role in reducing lesional and plasma HIV-1 levels [<xref ref-type="bibr" rid="ref6">6</xref>
, <xref ref-type="bibr" rid="ref21">21</xref>
]. Second, the strong association of HIV transmission with genital ulcer disease [<xref ref-type="bibr" rid="ref31">31</xref>
, <xref ref-type="bibr" rid="ref32">32</xref>
] suggests that a substantial proportion of HIV transmissions may take place during clinical ulcer episodes, including in settings where the majority of ulcers are caused by HSV-2 [<xref ref-type="bibr" rid="ref22">22</xref>
]. Shortening the duration of ulcerative episodes may therefore have an appreciable effect on HIV transmission [<xref ref-type="bibr" rid="ref31">31</xref>
, <xref ref-type="bibr" rid="ref33">33</xref>
]. Antibiotic treatment reduces HIV shedding in men with urethritis [<xref ref-type="bibr" rid="ref34">34</xref>
], and, in community randomized trials, it also reduces the incidence and prevalence of ulcer-causing infections [<xref ref-type="bibr" rid="ref35">35</xref>
, <xref ref-type="bibr" rid="ref36">36</xref>
] as well as the incidence of HIV [<xref ref-type="bibr" rid="ref37">37</xref>
], although it is unclear whether the latter was mediated through an effect in reducing HIV susceptibility, HIV infectiousness, or both. Because the majority of ulcers in developing countries are caused by HSV-2 [<xref ref-type="bibr" rid="ref3">3</xref>
], particularly among HIV-positive patients, the World Health Organization recommends adding acyclovir to antibacterial syndromic management [<xref ref-type="bibr" rid="ref23">23</xref>
]. However, data on the effectiveness of this strategy in developing countries, where patients may present late (often with concomitant HIV infection), is lacking. Furthermore, few studies of episodic antiherpetic therapy provided to HIV-positive patients have been conducted, even in industrialized countries [<xref ref-type="bibr" rid="ref6">6</xref>
, <xref ref-type="bibr" rid="ref38">38</xref>
]. Thus, we measured the impact of episodic HSV treatment in a pragmatic trial. Our results suggest that currently recommended HSV therapy does not dramatically improve ulcer healing, has only a modest effect on HSV-2 shedding, and could not inhibit the replication of HIV-1 in the genital tract or plasma</p>
<p>The estimated adherence to the study drug, as measured by pill counts, was very high, and the acyclovir dosage and duration of therapy used were the same as those specified in current treatment guidelines for recurrent genital herpetic ulceration [<xref ref-type="bibr" rid="ref23">23</xref>
, <xref ref-type="bibr" rid="ref25">25</xref>
]. Given that only 15%–30% of orally administered acyclovir is absorbed [<xref ref-type="bibr" rid="ref39">39</xref>
, <xref ref-type="bibr" rid="ref40">40</xref>
], it may be that this regimen, which has been studied mostly among HIV-uninfected patients, is insufficient to promote ulcer healing and reduce HSV-2 shedding (and, by implication, HIV-1 replication) among HIV-infected patients, many of whom have substantial immunosuppression. In an international multicenter trial conducted among HIV-infected patients, Conant et al. [<xref ref-type="bibr" rid="ref38">38</xref>
] did not find any difference in ulcer healing between patients taking 1000 mg of valacyclovir twice daily (>50% of which is absorbed) or 200 mg of acyclovir 5 times daily for 5 days, with a median time to healing of 5 days in both treatment groups. This was 2 days less than the median time to ulcer healing noted among women taking acyclovir in the present study. The fact that the level of immunosuppression among HIV-1–infected women in the present study was more pronounced than that noted in the study by Conant et al. [<xref ref-type="bibr" rid="ref38">38</xref>
] (by an average increase of 50 cells/mm<sup>3</sup>
) may have contributed to slow reepithelialization. Prolonged therapy (duration, ⩾10 days) [<xref ref-type="bibr" rid="ref25">25</xref>
] or a higher dosage of antivirals may be necessary for more immunocompromised individuals</p>
<p>Early initiation of therapy (within 24–48 h of the onset of the lesion) is required for optimal control of HSV-2, because peak shedding of HSV-2 is generally observed within 24 h of the onset of the lesion [<xref ref-type="bibr" rid="ref41">41</xref>
]. Thus, the modest effect on HSV-2 genital shedding and ulcer healing noted in the present study may be partly attributable to the long delay before the start of acyclovir after the onset of lesions, although our subgroup analysis suggested a stronger effect of acyclovir on HSV-2 shedding among patients presenting >7 days after onset of the lesion. Such delays are probably representative of most resource-poor settings, where, given limited awareness and accessibility of medical services and herpes treatment, a selection process might have biased recruitment to patients with more severe cases, whereas more-immunocompetent patients preferentially wait for spontaneous resolution of their ulcer</p>
<p>The results of the present study indicate that management of herpetic clinical episodes was unable to reduce systemic and genital HIV-1 replication. By contrast, recent trials of daily suppressive antiviral therapy for HSV-2 in patients coinfected with HSV-2 and HIV have shown an effect on both genital and plasma HIV-1 RNA loads among asymptomatic patients [<xref ref-type="bibr" rid="ref12">12</xref>
<xref ref-type="bibr" rid="ref13"></xref>
<xref ref-type="bibr" rid="ref14"></xref>
<xref ref-type="bibr" rid="ref15"></xref>
–<xref ref-type="bibr" rid="ref16">16</xref>
], with some exceptions [<xref ref-type="bibr" rid="ref42">42</xref>
, <xref ref-type="bibr" rid="ref43">43</xref>
], thereby confirming the causal relationship between HSV reactivation and HIV replication. The postulated mechanism of impact is that, by reducing reactivations of HSV-2, herpes-suppressive therapy is able to prevent HIV replication by disrupting direct viral interactions and, indirectly, by reducing the systemic and local immune activation induced by HSV-2 [<xref ref-type="bibr" rid="ref11">11</xref>
]. HSV-2 genital shedding is associated with increased levels of HIV-1 target cells (i.e., activated CD4 T lymphocytes and immature dendritic cells) within the genital mucosa [<xref ref-type="bibr" rid="ref44">44</xref>
, <xref ref-type="bibr" rid="ref45">45</xref>
], and recent studies have shown that an HSV-2–specific cellular immune response persists in the genital mucosa after resolution of herpes lesions [<xref ref-type="bibr" rid="ref46">46</xref>
]. This response may increase replication of genital HIV-1 in HIV-1–infected individuals. In addition, recent ex vivo experiments have suggested that acyclovir may directly inhibit HIV replication in tissues coinfected with HSV and HIV [<xref ref-type="bibr" rid="ref47">47</xref>
]. However, our data suggest that such an effect may not be potent enough in vivo or is not sustained long enough to have a major impact</p>
<p>Our study had a number of limitations. It was designed as a pragmatic trial, in which unselected patients presenting with genital ulcers were randomized regardless of their HIV status and HSV status, because these could not be determined before randomization. Therefore, our primary analysis included only a subgroup of the randomized patients, and this resulted, by chance, in unbalanced groups for some baseline characteristics. In particular, women in the acyclovir group were more likely to have detectable plasma, lesional, and cervicovaginal HIV-1 RNA at baseline. As specified in our analysis plan, we adjusted for these baseline imbalances when estimating effect, but the direction of these imbalances may have led to underestimation of the true impact of the intervention. Our final sample size was also slightly smaller than that estimated by the power calculations (118 vs. 135 patients), and this fact, combined with an imbalance in numbers in each group, would have slightly reduced our power to detect significant differences. Finally, exclusion (for ethical reasons) of patients with more-severe herpetic ulcers may limit the generalizability of our findings, because such patients may more readily experience benefits from acyclovir therapy</p>
<p>In conclusion, our results indicate that, among HIV-infected women, the use of acyclovir in accordance with current recommendations for the management of genital ulcer disease due to HSV-2 had only a weak effect on the course of the ulcers and HSV-2 genital shedding, and it did not reduce systemic and genital HIV replication. Our results do not support the hypothesis that provider-initiated short-course acyclovir therapy effectively disrupts genital interactions between HIV-1 and HSV-2 or that such treatment has any profound effect on genital HIV-1 transmissibility. Trials of longer regimens or alternative antivirals, such as oral valacyclovir, would be useful to support the inclusion of herpes episodic therapy in the management of genital ulcer disease. Offering herpes-suppressive therapy to HIV-infected individuals presenting with genital ulcers might help reduce the frequency of clinical recurrences and the effect on transmission and progression of HIV-1</p>
</sec>
<sec id="S4">
<title>Study Group Members</title>
<p>Members of the ARNS 1212 Study Group (by affiliation[s]) are as follows: L.B., H.B., Cécile Chemin, Maxime Lecerf, J.LeG., and Ali Si-Mohamed (Université Paris V, Equipe “Immunité et Biothérapie Muqueuse,” Unité INSERM Internationale U743 [“Immunologie Humaine”], Centre de Recherches Biomédicales des Cordeliers and Laboratoire de Virologie, Hôpital Européen Georges Pompidou, Paris, France); R.J.H., D.C.W.M., P.M., and H.A.W. (Clinical Research Unit, Department of Infectious and Tropical Diseases, and Infectious Disease Epidemiology Unit, Department of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, London, United Kingdom); G.G. and Jean-de-Dieu Longo (Centre National de Référence des Maladies Sexuellement Transmissibles et du SIDA de Bangui, & Unité de Recherches et d’Intervention sur les Maladies Sexuellement Transmissibles et du SIDA, Faculté des Sciences de la Santé, Bangui, Central African Republic); Thomas Agyarko-Poku, Comfort Asamoah-Adu, Agnes Dzokoto, and K.N. (West African Project to Combat AIDS and STDs, Accra, Ghana); Centre for International Health, University of Sherbrooke, Sherbrooke, Canada (Sylvie Deslandes, E.F., and J.P.); and J.-E.M. (Centre Médical, Institut Pasteur, Paris, France)</p>
</sec>
</body>
<back>
<ack>
<title>Acknowledgments</title>
<p>We thank the women who participated in this study; the staff from the Centre National de Référence des Maladies Sexuellement Transmissibles et du SIDA (Bangui, Ghana); and the West African Project to Combat AIDS and STIs (Accra and Kumasi, Central African Republic). We also thank Michel Kazatchkine, Brigitte Bazin, and Séverine Blesson (Agence Nationale de Recherches sur le SIDA et les Hépatites) and Jamie Robinson (GlaxoSmithKline) for their support throughout the study. We are also grateful to members of the Data Monitoring Committee (Peter Smith [chair] and Tim Clayton [both from the London School of Hygiene & Tropical Medicine] and Anne Johnson [from the Royal Free Hospital, London]) and to Francis Ndowa (from the Reproductive Health Research Division, STI Unit, World Health Organization, Geneva) for their advice</p>
<p><bold><italic>Financial support</italic>
</bold>
Agence Nationale de Recherches sur le SIDA et les Hépatites (ANRS), Paris (ANRS 1212); ANRS Clinical Monitor contract to H.B; GlaxoSmith Kline (GSK) Research & Development Department, Worldwide Epidemiology, Greenford, United Kingdom (GSK had no role in the manufacture and distribution of study drugs or on the review and analysis of trial results); and the United Kingdom Department for International Development–funded Research Programme Consortium on Research and Capacity Building on Sexual & Reproductive Health and HIV in Developing Countries</p>
<p><bold><italic>Supporting information</italic>
</bold>
Consolidated Standards of Reporting Trials Checklist; Agence Nationale de Recherches sur le SIDA et les Hépatites 1212 Trial Plan of Analysis</p>
</ack>
<ref-list>
<title>References</title>
<ref id="ref1">
<label>1</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Weiss</surname>
<given-names>H</given-names>
</name>
</person-group>
<article-title>Epidemiology of herpes simplex virus type 2 infection in the developing world</article-title>
<source>Herpes</source>
<year>2004</year>
<volume>11</volume>
<fpage>24A</fpage>
<lpage>35A</lpage>
</nlm-citation>
</ref>
<ref id="ref2">
<label>2</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Malkin</surname>
<given-names>JE</given-names>
</name>
</person-group>
<article-title>Epidemiology of genital herpes simplex virus infection in developed countries</article-title>
<source>Herpes</source>
<year>2004</year>
<volume>11</volume>
<fpage>2A</fpage>
<lpage>23A</lpage>
</nlm-citation>
</ref>
<ref id="ref3">
<label>3</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Paz-Bailey</surname>
<given-names>G</given-names>
</name>
<name><surname>Ramaswamy</surname>
<given-names>M</given-names>
</name>
<name><surname>Hawkes</surname>
<given-names>SJ</given-names>
</name>
<name><surname>Geretti</surname>
<given-names>AM</given-names>
</name>
</person-group>
<article-title>Herpes simplex virus type 2: epidemiology and management options in developing countries</article-title>
<source>Sex Transm Infect</source>
<year>2007</year>
<volume>83</volume>
<fpage>16</fpage>
<lpage>22</lpage>
</nlm-citation>
</ref>
<ref id="ref4">
<label>4</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Wald</surname>
<given-names>A</given-names>
</name>
<name><surname>Link</surname>
<given-names>K</given-names>
</name>
</person-group>
<article-title>Risk of human immunodeficiency virus infection in herpes simplex virus type 2–seropositive persons: a meta-analysis</article-title>
<source>J Infect Dis</source>
<year>2002</year>
<volume>185</volume>
<fpage>45</fpage>
<lpage>52</lpage>
</nlm-citation>
</ref>
<ref id="ref5">
<label>5</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Cross</surname>
<given-names>PL</given-names>
</name>
<name><surname>Whitworth</surname>
<given-names>JA</given-names>
</name>
</person-group>
<article-title>Herpes simplex virus 2 infection increases HIV acquisition in men and women: systematic review and meta-analysis of longitudinal studies</article-title>
<source>AIDS</source>
<year>2006</year>
<volume>20</volume>
<fpage>73</fpage>
<lpage>83</lpage>
</nlm-citation>
</ref>
<ref id="ref6">
<label>6</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Schacker</surname>
<given-names>T</given-names>
</name>
<name><surname>Ryncarz</surname>
<given-names>AJ</given-names>
</name>
<name><surname>Goddard</surname>
<given-names>J</given-names>
</name>
<name><surname>Diem</surname>
<given-names>K</given-names>
</name>
<name><surname>Shaughnessy</surname>
<given-names>M</given-names>
</name>
</person-group>
<article-title>Frequent recovery of HIV-1 from genital herpes simplex virus lesions in HIV-1-infected men</article-title>
<source>JAMA</source>
<year>1998</year>
<volume>280</volume>
<fpage>61</fpage>
<lpage>6</lpage>
</nlm-citation>
</ref>
<ref id="ref7">
<label>7</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>LeGoff</surname>
<given-names>J</given-names>
</name>
<name><surname>Weiss</surname>
<given-names>HA</given-names>
</name>
<name><surname>Gresenguet</surname>
<given-names>G</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Cervicovaginal HIV-1 and herpes simplex virus type 2 shedding during genital ulcer disease episodes</article-title>
<source>AIDS</source>
<year>2007</year>
<volume>21</volume>
<fpage>1569</fpage>
<lpage>78</lpage>
</nlm-citation>
</ref>
<ref id="ref8">
<label>8</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Mbopi-Kéou</surname>
<given-names>FX</given-names>
</name>
<name><surname>Grésenguet</surname>
<given-names>G</given-names>
</name>
<name><surname>Mayaud</surname>
<given-names>P</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Interactions between herpes simplex virus type 2 and human immunodeficiency virus type 1 infection in African women: opportunities for intervention</article-title>
<source>J Infect Dis</source>
<year>2000</year>
<volume>182</volume>
<fpage>1090</fpage>
<lpage>6</lpage>
</nlm-citation>
</ref>
<ref id="ref9">
<label>9</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>McClelland</surname>
<given-names>RS</given-names>
</name>
<name><surname>Wang</surname>
<given-names>CC</given-names>
</name>
<name><surname>Overbaugh</surname>
<given-names>J</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Association between cervical shedding of herpes simplex virus and HIV-1</article-title>
<source>AIDS</source>
<year>2002</year>
<volume>16</volume>
<fpage>2425</fpage>
<lpage>30</lpage>
</nlm-citation>
</ref>
<ref id="ref10">
<label>10</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Nagot</surname>
<given-names>N</given-names>
</name>
<name><surname>Ouedraogo</surname>
<given-names>A</given-names>
</name>
<name><surname>Konate</surname>
<given-names>I</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Roles of clinical and subclinical reactivated herpes simplex virus type 2 infection and human immunodeficiency virus type 1 (HIV-1)–induced immunosuppression on genital and plasma HIV-1 levels</article-title>
<source>J Infect Dis</source>
<year>2008</year>
<volume>198</volume>
<fpage>241</fpage>
<lpage>9</lpage>
</nlm-citation>
</ref>
<ref id="ref11">
<label>11</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Van de Perre</surname>
<given-names>P</given-names>
</name>
<name><surname>Segondy</surname>
<given-names>M</given-names>
</name>
<name><surname>Foulongne</surname>
<given-names>V</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Herpes simplex virus and HIV-1: deciphering viral synergy</article-title>
<source>Lancet Infect Dis</source>
<year>2008</year>
<volume>8</volume>
<fpage>490</fpage>
<lpage>7</lpage>
</nlm-citation>
</ref>
<ref id="ref12">
<label>12</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Delany</surname>
<given-names>S</given-names>
</name>
<name><surname>Mlaba</surname>
<given-names>N</given-names>
</name>
<name><surname>Clayton</surname>
<given-names>T</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Impact of aciclovir on genital and plasma HIV-1 RNA in HSV-2 and HIV-1 co-infected women: a randomized placebo-controlled trial in South Africa</article-title>
<source>AIDS</source>
<year>2009</year>
<volume>23</volume>
<fpage>461</fpage>
<lpage>9</lpage>
</nlm-citation>
</ref>
<ref id="ref13">
<label>13</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Dunne</surname>
<given-names>EF</given-names>
</name>
<name><surname>Whitehead</surname>
<given-names>S</given-names>
</name>
<name><surname>Sternberg</surname>
<given-names>M</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Suppressive acyclovir therapy reduces HIV cervicovaginal shedding in HIV- and HSV-2-infected women, Chiang Rai, Thailand</article-title>
<source>J Acquir Immune Defic Syndr</source>
<year>2008</year>
<volume>49</volume>
<fpage>77</fpage>
<lpage>83</lpage>
</nlm-citation>
</ref>
<ref id="ref14">
<label>14</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Nagot</surname>
<given-names>N</given-names>
</name>
<name><surname>Ouédraogo</surname>
<given-names>A</given-names>
</name>
<name><surname>Foulongne</surname>
<given-names>V</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Reduction of HIV-1 RNA levels with therapy to suppress herpes simplex virus</article-title>
<source>N Engl J Med</source>
<year>2007</year>
<volume>356</volume>
<fpage>790</fpage>
<lpage>9</lpage>
</nlm-citation>
</ref>
<ref id="ref15">
<label>15</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Zuckerman</surname>
<given-names>RA</given-names>
</name>
<name><surname>Lucchetti</surname>
<given-names>A</given-names>
</name>
<name><surname>Whittington</surname>
<given-names>WL</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Herpes simplex virus (HSV) suppression with valacyclovir reduces rectal and blood plasma HIV-1 levels in HIV-1/HSV-2–seropositive men: a randomized, double-blind, placebo-controlled crossover trial</article-title>
<source>J Infect Dis</source>
<year>2007</year>
<volume>196</volume>
<fpage>1500</fpage>
<lpage>8</lpage>
</nlm-citation>
</ref>
<ref id="ref16">
<label>16</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Baeten</surname>
<given-names>JM</given-names>
</name>
<name><surname>Strick</surname>
<given-names>LB</given-names>
</name>
<name><surname>Lucchetti</surname>
<given-names>A</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Herpes simplex virus (HSV)–suppressive therapy decreases plasma and genital HIV-1 levels in HSV-2/HIV-1 coinfected women: a randomized, placebo-controlled, cross-over trial</article-title>
<source>J Infect Dis</source>
<year>2008</year>
<volume>198</volume>
<fpage>1804</fpage>
<lpage>8</lpage>
</nlm-citation>
</ref>
<ref id="ref17">
<label>17</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Celum</surname>
<given-names>C</given-names>
</name>
<name><surname>Levine</surname>
<given-names>R</given-names>
</name>
<name><surname>Weaver</surname>
<given-names>M</given-names>
</name>
<name><surname>Wald</surname>
<given-names>A</given-names>
</name>
</person-group>
<article-title>Genital herpes and human immunodeficiency virus: double trouble</article-title>
<source>Bull World Health Organ</source>
<year>2004</year>
<volume>82</volume>
<fpage>447</fpage>
<lpage>53</lpage>
</nlm-citation>
</ref>
<ref id="ref18">
<label>18</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Corey</surname>
<given-names>L</given-names>
</name>
<name><surname>Wald</surname>
<given-names>A</given-names>
</name>
<name><surname>Celum</surname>
<given-names>CL</given-names>
</name>
<name><surname>Quinn</surname>
<given-names>TC</given-names>
</name>
</person-group>
<article-title>The effects of herpes simplex virus-2 on HIV-1 acquisition and transmission: a review of two overlapping epidemics</article-title>
<source>J Acquir Immune Defic Syndr</source>
<year>2004</year>
<volume>35</volume>
<fpage>435</fpage>
<lpage>45</lpage>
</nlm-citation>
</ref>
<ref id="ref19">
<label>19</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Siegal</surname>
<given-names>FP</given-names>
</name>
<name><surname>Lopez</surname>
<given-names>C</given-names>
</name>
<name><surname>Hammer</surname>
<given-names>GS</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Severe acquired immunodeficiency in male homosexuals, manifested by chronic perianal ulcerative herpes simplex lesions</article-title>
<source>N Engl J Med</source>
<year>1981</year>
<volume>305</volume>
<fpage>1439</fpage>
<lpage>44</lpage>
</nlm-citation>
</ref>
<ref id="ref20">
<label>20</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Mole</surname>
<given-names>L</given-names>
</name>
<name><surname>Ripich</surname>
<given-names>S</given-names>
</name>
<name><surname>Margolis</surname>
<given-names>D</given-names>
</name>
<name><surname>Holodniy</surname>
<given-names>M</given-names>
</name>
</person-group>
<article-title>The impact of active herpes simplex virus infection on human immunodeficiency virus load</article-title>
<source>J Infect Dis</source>
<year>1997</year>
<volume>176</volume>
<fpage>766</fpage>
<lpage>70</lpage>
</nlm-citation>
</ref>
<ref id="ref21">
<label>21</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Schacker</surname>
<given-names>T</given-names>
</name>
<name><surname>Zeh</surname>
<given-names>J</given-names>
</name>
<name><surname>Hu</surname>
<given-names>H</given-names>
</name>
<name><surname>Shaughnessy</surname>
<given-names>M</given-names>
</name>
<name><surname>Corey</surname>
<given-names>L</given-names>
</name>
</person-group>
<article-title>Changes in plasma human immunodeficiency virus type 1 RNA associated with herpes simplex virus reactivation and suppression</article-title>
<source>J Infect Dis</source>
<year>2002</year>
<volume>186</volume>
<fpage>1718</fpage>
<lpage>25</lpage>
</nlm-citation>
</ref>
<ref id="ref22">
<label>22</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Gray</surname>
<given-names>RH</given-names>
</name>
<name><surname>Wawer</surname>
<given-names>MJ</given-names>
</name>
<name><surname>Brookmeyer</surname>
<given-names>R</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Probability of HIV-1 transmission per coital act in monogamous, heterosexual, HIV-1-discordant couples in Rakai, Uganda</article-title>
<source>Lancet</source>
<year>2001</year>
<volume>357</volume>
<fpage>1149</fpage>
<lpage>53</lpage>
</nlm-citation>
</ref>
<ref id="ref23">
<label>23</label>
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<collab>World Health Organization (WHO)</collab>
</person-group>
<article-title>Guidelines for the management of sexually transmitted infections</article-title>
<year>2003</year>
<publisher-loc>Geneva</publisher-loc>
<publisher-name>WHO</publisher-name>
</nlm-citation>
</ref>
<ref id="ref24">
<label>24</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<collab>Sexually transmitted diseases treatment guidelines 2002</collab>
</person-group>
<article-title>Centers for Disease Control and Prevention</article-title>
<source>MMWR Morb Mortal Wkly Rep</source>
<year>2002</year>
<volume>51</volume>
<fpage>1</fpage>
<lpage>78</lpage>
</nlm-citation>
</ref>
<ref id="ref25">
<label>25</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Workowski</surname>
<given-names>KA</given-names>
</name>
<name><surname>Berman</surname>
<given-names>SM</given-names>
</name>
</person-group>
<article-title>Sexually transmitted diseases treatment guidelines, 2006</article-title>
<source>MMWR Recomm Rep</source>
<year>2006</year>
<volume>55</volume>
<fpage>1</fpage>
<lpage>94</lpage>
</nlm-citation>
</ref>
<ref id="ref26">
<label>26</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Belec</surname>
<given-names>L</given-names>
</name>
<name><surname>Meillet</surname>
<given-names>D</given-names>
</name>
<name><surname>Lévy</surname>
<given-names>M</given-names>
</name>
<name><surname>Georges</surname>
<given-names>A</given-names>
</name>
<name><surname>Tévi-Bénissan</surname>
<given-names>C</given-names>
</name>
</person-group>
<article-title>Dilution assessment of cervicovaginal secretions obtained by vaginal washing for immunological assays</article-title>
<source>Clin Diagn Lab Immunol</source>
<year>1995</year>
<volume>2</volume>
<fpage>57</fpage>
<lpage>61</lpage>
</nlm-citation>
</ref>
<ref id="ref27">
<label>27</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>LeGoff</surname>
<given-names>J</given-names>
</name>
<name><surname>Mayaud</surname>
<given-names>P</given-names>
</name>
<name><surname>Gresenguet</surname>
<given-names>G</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Performance of HerpeSelect and Kalon assays in detection of antibodies to herpes simplex virus type 2</article-title>
<source>J Clin Microbiol</source>
<year>2008</year>
<volume>46</volume>
<fpage>1914</fpage>
<lpage>8</lpage>
</nlm-citation>
</ref>
<ref id="ref28">
<label>28</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Legoff</surname>
<given-names>J</given-names>
</name>
<name><surname>Bouhlal</surname>
<given-names>H</given-names>
</name>
<name><surname>Grésenguet</surname>
<given-names>G</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Real-time PCR quantification of genital shedding of herpes simplex virus (HSV) and human immunodeficiency virus (HIV) in women coinfected with HSV and HIV</article-title>
<source>J Clin Microbiol</source>
<year>2006</year>
<volume>44</volume>
<fpage>423</fpage>
<lpage>32</lpage>
</nlm-citation>
</ref>
<ref id="ref29">
<label>29</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Zou</surname>
<given-names>G</given-names>
</name>
</person-group>
<article-title>A modified Poisson regression approach to prospective studies with binary data</article-title>
<source>Am J Epidemiol</source>
<year>2004</year>
<volume>159</volume>
<fpage>702</fpage>
<lpage>6</lpage>
</nlm-citation>
</ref>
<ref id="ref30">
<label>30</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>McClelland</surname>
<given-names>RS</given-names>
</name>
<name><surname>Wang</surname>
<given-names>CC</given-names>
</name>
<name><surname>Richardson</surname>
<given-names>BA</given-names>
</name>
<etal></etal>
</person-group>
<article-title>A prospective study of hormonal contraceptive use and cervical shedding of herpes simplex virus in human immunodeficiency virus type 1–seropositive women</article-title>
<source>J Infect Dis</source>
<year>2002</year>
<volume>185</volume>
<fpage>1822</fpage>
<lpage>5</lpage>
</nlm-citation>
</ref>
<ref id="ref31">
<label>31</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Hayes</surname>
<given-names>RJ</given-names>
</name>
<name><surname>Schulz</surname>
<given-names>KF</given-names>
</name>
<name><surname>Plummer</surname>
<given-names>FA</given-names>
</name>
</person-group>
<article-title>The cofactor effect of genital ulcers on the per-exposure risk of HIV transmission in sub-Saharan Africa</article-title>
<source>J Trop Med Hyg</source>
<year>1995</year>
<volume>98</volume>
<fpage>1</fpage>
<lpage>8</lpage>
</nlm-citation>
</ref>
<ref id="ref32">
<label>32</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Robinson</surname>
<given-names>NJ</given-names>
</name>
<name><surname>Mulder</surname>
<given-names>DW</given-names>
</name>
<name><surname>Auvert</surname>
<given-names>B</given-names>
</name>
<name><surname>Hayes</surname>
<given-names>RJ</given-names>
</name>
</person-group>
<article-title>Proportion of HIV infections attributable to other sexually transmitted diseases in a rural Ugandan population: simulation model estimates</article-title>
<source>Int J Epidemiol</source>
<year>1997</year>
<volume>26</volume>
<fpage>180</fpage>
<lpage>9</lpage>
</nlm-citation>
</ref>
<ref id="ref33">
<label>33</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<etal></etal>
</person-group>
<article-title>Treating curable sexually transmitted infections to prevent HIV in Africa: still an effective control strategy</article-title>
<source>J Acquir Immune Defic Syndr</source>
<year>2008</year>
<volume>47</volume>
<fpage>346</fpage>
<lpage>53</lpage>
</nlm-citation>
</ref>
<ref id="ref34">
<label>34</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Cohen</surname>
<given-names>MS</given-names>
</name>
<name><surname>Hoffman</surname>
<given-names>IF</given-names>
</name>
<name><surname>Royce</surname>
<given-names>RA</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Reduction of concentration of HIV-1 in semen after treatment of urethritis: implications for prevention of sexual transmission of HIV-1. AIDSCAP Malawi Research Group</article-title>
<source>Lancet</source>
<year>1997</year>
<volume>349</volume>
<fpage>1868</fpage>
<lpage>73</lpage>
</nlm-citation>
</ref>
<ref id="ref35">
<label>35</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Mayaud</surname>
<given-names>P</given-names>
</name>
<name><surname>Mosha</surname>
<given-names>F</given-names>
</name>
<name><surname>Todd</surname>
<given-names>J</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Improved treatment services significantly reduce the prevalence of sexually transmitted diseases in rural Tanzania: results of a randomized controlled trial</article-title>
<source>AIDS</source>
<year>1997</year>
<volume>11</volume>
<fpage>1873</fpage>
<lpage>80</lpage>
</nlm-citation>
</ref>
<ref id="ref36">
<label>36</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Kamali</surname>
<given-names>A</given-names>
</name>
<name><surname>Quigley</surname>
<given-names>M</given-names>
</name>
<name><surname>Nakiyingi</surname>
<given-names>J</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Syndromic management of sexually-transmitted infections and behaviour change interventions on transmission of HIV-1 in rural Uganda: a community randomised trial</article-title>
<source>Lancet</source>
<year>2003</year>
<volume>361</volume>
<fpage>645</fpage>
<lpage>52</lpage>
</nlm-citation>
</ref>
<ref id="ref37">
<label>37</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Grosskurth</surname>
<given-names>H</given-names>
</name>
<name><surname>Mosha</surname>
<given-names>F</given-names>
</name>
<name><surname>Todd</surname>
<given-names>J</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Impact of improved treatment of sexually transmitted diseases on HIV infection in rural Tanzania: randomised controlled trial</article-title>
<source>Lancet</source>
<year>1995</year>
<volume>346</volume>
<fpage>530</fpage>
<lpage>6</lpage>
</nlm-citation>
</ref>
<ref id="ref38">
<label>38</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Conant</surname>
<given-names>MA</given-names>
</name>
<name><surname>Schacker</surname>
<given-names>TW</given-names>
</name>
<name><surname>Murphy</surname>
<given-names>RL</given-names>
</name>
<name><surname>Gold</surname>
<given-names>J</given-names>
</name>
<name><surname>Crutchfield</surname>
<given-names>LT</given-names>
</name>
</person-group>
<article-title>Valaciclovir versus aciclovir for herpes simplex virus infection in HIV-infected individuals: two randomized trials</article-title>
<source>Int J STD AIDS</source>
<year>2002</year>
<volume>13</volume>
<fpage>12</fpage>
<lpage>21</lpage>
</nlm-citation>
</ref>
<ref id="ref39">
<label>39</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Laskin</surname>
<given-names>OL</given-names>
</name>
</person-group>
<article-title>Clinical pharmacokinetics of acyclovir</article-title>
<source>Clin Pharmacokinet</source>
<year>1983</year>
<volume>8</volume>
<fpage>187</fpage>
<lpage>201</lpage>
</nlm-citation>
</ref>
<ref id="ref40">
<label>40</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Wagstaff</surname>
<given-names>AJ</given-names>
</name>
<name><surname>Faulds</surname>
<given-names>D</given-names>
</name>
<name><surname>Goa</surname>
<given-names>KL</given-names>
</name>
</person-group>
<article-title>Aciclovir: a reappraisal of its antiviral activity, pharmacokinetic properties and therapeutic efficacy</article-title>
<source>Drugs</source>
<year>1994</year>
<volume>47</volume>
<fpage>153</fpage>
<lpage>205</lpage>
</nlm-citation>
</ref>
<ref id="ref41">
<label>41</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Brown</surname>
<given-names>ZA</given-names>
</name>
<name><surname>Kern</surname>
<given-names>ER</given-names>
</name>
<name><surname>Spruance</surname>
<given-names>SL</given-names>
</name>
</person-group>
<article-title>Overall JC Jr. Clinical and virologic course of herpes simplex genitalis</article-title>
<source>West J Med</source>
<year>1979</year>
<volume>130</volume>
<fpage>414</fpage>
<lpage>21</lpage>
</nlm-citation>
</ref>
<ref id="ref42">
<label>42</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Cowan</surname>
<given-names>FM</given-names>
</name>
<name><surname>Pascoe</surname>
<given-names>S</given-names>
</name>
<name><surname>Barlow</surname>
<given-names>K</given-names>
</name>
<etal></etal>
</person-group>
<article-title>A randomised placebo controlled trial to explore the effect of suppressive therapy with acyclovir on genital shedding of HIV-1 and herpes simplex virus type 2 among Zimbabwean sex workers</article-title>
<source>Sex Transm Infect</source>
<year>2008</year>
<volume>84</volume>
<fpage>548</fpage>
<lpage>53</lpage>
</nlm-citation>
</ref>
<ref id="ref43">
<label>43</label>
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name><surname>Tanton</surname>
<given-names>C</given-names>
</name>
<name><surname>Watson-Jones</surname>
<given-names>D</given-names>
</name>
<name><surname>Rusizoka</surname>
<given-names>M</given-names>
</name>
<etal></etal>
</person-group>
<article-title>A randomised controlled trial in Tanzania to assess the impact of HSV-2 suppressive therapy on genital HIV viral load among HSV-2 and HIV-1 seropositive women (abstract O-099)</article-title>
<source>Program and abstracts of the 17th Conference of the International Society for Sexually Transmitted Diseases Research</source>
<year>2007</year>
<publisher-loc>Seattle, Washington</publisher-loc>
<publisher-name>ISSTDR</publisher-name>
</nlm-citation>
</ref>
<ref id="ref44">
<label>44</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Rebbapragada</surname>
<given-names>A</given-names>
</name>
<name><surname>Wachihi</surname>
<given-names>C</given-names>
</name>
<name><surname>Pettengell</surname>
<given-names>C</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Negative mucosal synergy between herpes simplex type 2 and HIV in the female genital tract</article-title>
<source>AIDS</source>
<year>2007</year>
<volume>21</volume>
<fpage>589</fpage>
<lpage>98</lpage>
</nlm-citation>
</ref>
<ref id="ref45">
<label>45</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Kaul</surname>
<given-names>R</given-names>
</name>
<name><surname>Pettengell</surname>
<given-names>C</given-names>
</name>
<name><surname>Sheth</surname>
<given-names>PM</given-names>
</name>
<etal></etal>
</person-group>
<article-title>The genital tract immune milieu: an important determinant of HIV susceptibility and secondary transmission</article-title>
<source>J Reprod Immunol</source>
<year>2008</year>
<volume>77</volume>
<fpage>32</fpage>
<lpage>40</lpage>
</nlm-citation>
</ref>
<ref id="ref46">
<label>46</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Zhu</surname>
<given-names>J</given-names>
</name>
<name><surname>Koelle</surname>
<given-names>DM</given-names>
</name>
<name><surname>Cao</surname>
<given-names>J</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Virus-specific CD8<sup>+</sup>
T cells accumulate near sensory nerve endings in genital skin during subclinical HSV-2 reactivation</article-title>
<source>J Exp Med</source>
<year>2007</year>
<volume>204</volume>
<fpage>595</fpage>
<lpage>603</lpage>
</nlm-citation>
</ref>
<ref id="ref47">
<label>47</label>
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name><surname>Lisco</surname>
<given-names>A</given-names>
</name>
<name><surname>Vanpouille</surname>
<given-names>C</given-names>
</name>
<name><surname>Tchesnokov</surname>
<given-names>EP</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Acyclovir is activated into a HIV-1 reverse transcriptase inhibitor in herpesvirus-infected human tissues</article-title>
<source>Cell Host Microbe</source>
<year>2008</year>
<volume>4</volume>
<fpage>260</fpage>
<lpage>70</lpage>
</nlm-citation>
</ref>
</ref-list>
<sec sec-type="display-objects">
<title>Figures and Tables</title>
<fig id="Fig1" position="float">
<label>Figure 1</label>
<caption>
<p>Consolidated Standards of Reporting Trials flow diagram of eligibility, enrollment, and follow-up in the primary analysis group of the Agence Nationale de Recherches sur le SIDA et les Hépatites (ANRS) 1212 trial, which consisted of human immunodeficiency virus type 1 (HIV-1)–infected women with herpes simplex virus type 2 (HSV-2) ulcers. *Day 7 is the primary end point (defined as a visit occurring on days 6–8 after randomization). Eleven women in the control group and 6 women in the acyclovir treatment group were excluded from analyses because cervicovaginal lavage (CVL) specimens were not collected at this day 7 clinic visit, usually because of menstruation</p>
</caption>
<graphic mimetype="image" xlink:href="200-2-216-fig001.tif"></graphic>
</fig>
<fig id="Fig2" position="float">
<label>Figure 2</label>
<caption>
<p>Detection of cervicovaginal human immunodeficiency virus type 1 (HIV-1) RNA and herpes simplex virus type 2 (HSV-2) DNA in HIV-1–infected women with HSV-2 ulcers in Ghana and the Central African Republic over time</p>
</caption>
<graphic mimetype="image" xlink:href="200-2-216-fig002.tif"></graphic>
</fig>
<fig id="Fig3" position="float">
<label>Figure 3</label>
<caption>
<p>Rates of ulcer healing (as determined by Kaplan-Meier analysis) among 118 human immunodeficiency virus type 1–infected women with herpes simplex virus type 2 ulcers in Ghana and the Central African Republic. *P=.21 was estimated from Cox regression, with ulcer healing defined as a ⩾90% reduction in ulcer size. Nos. of patients at each visit are based on the no. of women whose ulcer size was recorded, which may be different from the no. of women who had cervicovaginal lavage specimens available, as shown in <xref ref-type="fig" rid="Fig1">figure 1</xref>
</p>
</caption>
<graphic mimetype="image" xlink:href="200-2-216-fig003.tif"></graphic>
</fig>
<fig id="tb1" position="float">
<label>Table 1</label>
<caption>
<p>Baseline characteristics of the primary analysis group: 118 human immunodeficiency virus type 1 (HIV-1)–infected women with herpes simplex virus type 2 (HSV-2) ulcers in Ghana and the Central African Republic (CAR)</p>
</caption>
<graphic mimetype="image" xlink:href="200-2-216-tab001.tif"></graphic>
</fig>
<fig id="tb2" position="float">
<label>Table 2</label>
<caption>
<p>Effect of acyclovir among human immunodeficiency virus type 1 (HIV-1)–infected women with herpes simplex virus type 2 (HSV-2) ulcers, by treatment group</p>
</caption>
<graphic mimetype="image" xlink:href="200-2-216-tab002.tif"></graphic>
</fig>
</sec>
<fn-group>
<fn id="fn1">
<p>Potential conflicts of interest: none reported</p>
<p>Presented in part: Annual Conference of the International Union against Sexually Transmitted Infections, Versailles, France, 19–21 October 2006 (oral presentation)</p>
<p>Financial support: Please see the Acknowledgments for information on financial support and supporting information</p>
</fn>
<fn id="fn2">
<p>Members of the study group are listed after the text</p>
</fn>
<fn id="fn3">
<p>The views presented in this manuscript are those of the authors and do not necessarily represent the official position of the United Kingdom Department of International Development</p>
</fn>
</fn-group>
</back>
</article>
</istex:document>
</istex:metadataXml>
<mods version="3.6"><titleInfo><title>Impact of Acyclovir on Genital and Plasma HIV-1 RNA, Genital Herpes Simplex Virus Type 2 DNA, and Ulcer Healing among HIV-1–Infected African Women with Herpes Ulcers: A Randomized Placebo-Controlled Trial</title>
</titleInfo>
<titleInfo type="alternative" contentType="CDATA"><title>Impact of Acyclovir on Genital and Plasma HIV-1 RNA, Genital Herpes Simplex Virus Type 2 DNA, and Ulcer Healing among HIV-1–Infected African Women with Herpes Ulcers: A Randomized Placebo-Controlled Trial</title>
</titleInfo>
<name type="corporate"><namePart>for the ANRS 1212 Study Group</namePart>
<affiliation>Reprints or correspondence: Philippe Mayaud, Clinical Research Unit, London School of Hygiene & Tropical Medicine, Keppel St., London WC1E 7HT, UK (philippe.mayaud@lshtm.ac.uk)</affiliation>
</name>
<name type="personal"><namePart type="given">Philippe</namePart>
<namePart type="family">Mayaud</namePart>
<affiliation>Clinical Research Unit, Department of Infectious and Tropical Diseases, and</affiliation>
<affiliation>Reprints or correspondence: Philippe Mayaud, Clinical Research Unit, London School of Hygiene & Tropical Medicine, Keppel St., London WC1E 7HT, UK (philippe.mayaud@lshtm.ac.uk)</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Jérôme</namePart>
<namePart type="family">LeGoff</namePart>
<affiliation>Université Paris Descartes, Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743 (Immunologie Humaine), Centre de Recherches Biomédicales des Cordeliers and Laboratoire de Virologie, Hôpital Européen Georges Pompidou,</affiliation>
<affiliation>Université Paris Diderot, Laboratoire de Microbiologie, Hôpital Saint-Louis, and</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Helen A.</namePart>
<namePart type="family">Weiss</namePart>
<affiliation>Infectious Disease Epidemiology Unit, Department of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, London, United Kingdom;</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Gérard</namePart>
<namePart type="family">Grésenguet</namePart>
<affiliation>Centre National de Référence des Maladies Sexuellement Transmissibles et du SIDA de Bangui and Unité de Recherches et d’Intervention sur les Maladies Sexuellement Transmissibles et du SIDA, Faculté des Sciences de la Santé, Bangui, Central African Republic;</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Khonde</namePart>
<namePart type="family">Nzambi</namePart>
<affiliation>West African Project To Combat AIDS and STIs, Accra, Ghana;</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Hicham</namePart>
<namePart type="family">Bouhlal</namePart>
<affiliation>Université Paris Descartes, Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743 (Immunologie Humaine), Centre de Recherches Biomédicales des Cordeliers and Laboratoire de Virologie, Hôpital Européen Georges Pompidou,</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Eric</namePart>
<namePart type="family">Frost</namePart>
<affiliation>Centre for International Health, University of Sherbrooke, Sherbrooke, Canada</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Jacques</namePart>
<namePart type="family">Pépin</namePart>
<affiliation>Centre for International Health, University of Sherbrooke, Sherbrooke, Canada</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Jean-Elie</namePart>
<namePart type="family">Malkin</namePart>
<affiliation>Centre Médical, Institut Pasteur, Paris, France;</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Richard J.</namePart>
<namePart type="family">Hayes</namePart>
<affiliation>Infectious Disease Epidemiology Unit, Department of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, London, United Kingdom;</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">David C. W.</namePart>
<namePart type="family">Mabey</namePart>
<affiliation>Clinical Research Unit, Department of Infectious and Tropical Diseases, and</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Laurent</namePart>
<namePart type="family">Bélec</namePart>
<affiliation>Université Paris Descartes, Equipe Immunité et Biothérapie Muqueuse, Unité INSERM Internationale U743 (Immunologie Humaine), Centre de Recherches Biomédicales des Cordeliers and Laboratoire de Virologie, Hôpital Européen Georges Pompidou,</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<typeOfResource>text</typeOfResource>
<genre type="research-article" displayLabel="research-article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre>
<originInfo><publisher>The University of Chicago Press</publisher>
<dateIssued encoding="w3cdtf">2009-07-15</dateIssued>
<copyrightDate encoding="w3cdtf">2009</copyrightDate>
</originInfo>
<abstract>BackgroundLittle is known about the impact of episodic treatment of herpes on human immunodeficiency virus type 1 (HIV-1) MethodsWomen from Ghana and the Central African Republic who had genital ulcers were enrolled in a randomized, double-blind, placebo-controlled trial of acyclovir plus antibacterials and were monitored for 28 days. Ulcer etiologies and detection of lesional HIV-1 RNA were determined by polymerase chain reaction (PCR). Cervicovaginal HIV-1 RNA and herpes simplex virus type 2 (HSV-2) DNA and plasma HIV-1 RNA were quantitated by real-time PCR. Primary analyses included 118 HIV-1–infected women with HSV-2 ulcers (54 of whom were given acyclovir and 64 of whom were given placebo) ResultsAcyclovir had little impact on (1) detection of cervicovaginal HIV-1 RNA (risk ratio [RR], 0.96; 95% confidence interval [CI], 0.8–1.2) at day 7 of treatment, (2) the mean cervicovaginal HIV-1 RNA load (−0.06 log10 copies/mL; 95% CI, −0.4 to 0.3 log10 copies/mL) at day 7 of treatment, or (3) the plasma HIV-1 RNA load (+0.09 log10 copies/mL; 95% CI, −0.1 to 0.3 log10 copies/mL) at day 14 of treatment. At day 7, women receiving acyclovir were less likely to have detectable lesional HIV-1 RNA (RR, 0.70; 95% CI, 0.4–1.2) or cervicovaginal HSV-2 DNA (RR, 0.69; 95% CI, 0.4–1.3), had a lower quantity of HSV-2 DNA (−0.99 log10 copies/mL; 95% CI, −1.8 to −0.2 log10 copies/mL), and were more likely to have a healed ulcer (RR, 1.26; 95% CI, 0.9–1.9) ConclusionEpisodic therapy for herpes reduced the quantity of cervicovaginal HSV-2 DNA and slightly improved ulcer healing, but it did not decrease genital and plasma HIV-1 RNA loads Trial registrationClinicalTrials.gov identifier NCT00158483</abstract>
<relatedItem type="host"><titleInfo><title>The Journal of Infectious Diseases</title>
</titleInfo>
<titleInfo type="abbreviated"><title>The Journal of Infectious Diseases</title>
</titleInfo>
<genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre>
<identifier type="ISSN">0022-1899</identifier>
<identifier type="eISSN">1537-6613</identifier>
<identifier type="PublisherID">jid</identifier>
<identifier type="PublisherID-hwp">jinfdis</identifier>
<part><date>2009</date>
<detail type="volume"><caption>vol.</caption>
<number>200</number>
</detail>
<detail type="issue"><caption>no.</caption>
<number>2</number>
</detail>
<extent unit="pages"><start>216</start>
<end>226</end>
</extent>
</part>
</relatedItem>
<identifier type="istex">A8D7F03BC8675B8AACE47022187702C7B7DE22EE</identifier>
<identifier type="DOI">10.1086/599991</identifier>
<accessCondition type="use and reproduction" contentType="copyright">© 2009 by the Infectious Diseases Society of America</accessCondition>
<recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-GTWS0RDP-M">oup</recordContentSource>
<recordOrigin>© 2009 by the Infectious Diseases Society of America</recordOrigin>
</recordInfo>
</mods>
<json:item><extension>json</extension>
<original>false</original>
<mimetype>application/json</mimetype>
<uri>https://api.istex.fr/document/A8D7F03BC8675B8AACE47022187702C7B7DE22EE/metadata/json</uri>
</json:item>
</metadata>
<covers><json:item><extension>tiff</extension>
<original>true</original>
<mimetype>image/tiff</mimetype>
<uri>https://api.istex.fr/document/A8D7F03BC8675B8AACE47022187702C7B7DE22EE/covers/tiff</uri>
</json:item>
<json:item><extension>html</extension>
<original>true</original>
<mimetype>text/html</mimetype>
<uri>https://api.istex.fr/document/A8D7F03BC8675B8AACE47022187702C7B7DE22EE/covers/html</uri>
</json:item>
</covers>
<annexes><json:item><extension>jpeg</extension>
<original>true</original>
<mimetype>image/jpeg</mimetype>
<uri>https://api.istex.fr/document/A8D7F03BC8675B8AACE47022187702C7B7DE22EE/annexes/jpeg</uri>
</json:item>
<json:item><extension>gif</extension>
<original>true</original>
<mimetype>image/gif</mimetype>
<uri>https://api.istex.fr/document/A8D7F03BC8675B8AACE47022187702C7B7DE22EE/annexes/gif</uri>
</json:item>
</annexes>
<serie></serie>
</istex>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/SidaSubSaharaV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003752 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 003752 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= SidaSubSaharaV1 |flux= Istex |étape= Corpus |type= RBID |clé= ISTEX:A8D7F03BC8675B8AACE47022187702C7B7DE22EE |texte= Impact of Acyclovir on Genital and Plasma HIV-1 RNA, Genital Herpes Simplex Virus Type 2 DNA, and Ulcer Healing among HIV-1–Infected African Women with Herpes Ulcers: A Randomized Placebo-Controlled Trial }}
![]() | This area was generated with Dilib version V0.6.32. | ![]() |