Trace Elements Affect Methanogenic Activity and Diversity in Enrichments from Subsurface Coal Bed Produced Water
Identifieur interne : 000034 ( Pmc/Curation ); précédent : 000033; suivant : 000035Trace Elements Affect Methanogenic Activity and Diversity in Enrichments from Subsurface Coal Bed Produced Water
Auteurs : Burcu Ünal [États-Unis] ; Verlin Ryan Perry [États-Unis] ; Mili Sheth [États-Unis] ; Vicente Gomez-Alvarez [États-Unis] ; Kuk-Jeong Chin [États-Unis] ; Klaus Nüsslein [États-Unis]Source :
- Frontiers in Microbiology [ 1664-302X ] ; 2012.
Abstract
Microbial methane from coal beds accounts for a significant and growing percentage of natural gas worldwide. Our knowledge of physical and geochemical factors regulating methanogenesis is still in its infancy. We hypothesized that in these closed systems, trace elements (as micronutrients) are a limiting factor for methanogenic growth and activity. Trace elements are essential components of enzymes or cofactors of metabolic pathways associated with methanogenesis. This study examined the effects of eight trace elements (iron, nickel, cobalt, molybdenum, zinc, manganese, boron, and copper) on methane production, on
Url:
DOI: 10.3389/fmicb.2012.00175
PubMed: 22590465
PubMed Central: 3349271
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<author><name sortKey="Nusslein, Klaus" sort="Nusslein, Klaus" uniqKey="Nusslein K" first="Klaus" last="Nüsslein">Klaus Nüsslein</name>
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<series><title level="j">Frontiers in Microbiology</title>
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<front><div type="abstract" xml:lang="en"><p>Microbial methane from coal beds accounts for a significant and growing percentage of natural gas worldwide. Our knowledge of physical and geochemical factors regulating methanogenesis is still in its infancy. We hypothesized that in these closed systems, trace elements (as micronutrients) are a limiting factor for methanogenic growth and activity. Trace elements are essential components of enzymes or cofactors of metabolic pathways associated with methanogenesis. This study examined the effects of eight trace elements (iron, nickel, cobalt, molybdenum, zinc, manganese, boron, and copper) on methane production, on <italic>mcrA</italic>
transcript levels, and on methanogenic community structure in enrichment cultures obtained from coal bed methane (CBM) well produced water samples from the Powder River Basin, Wyoming. Methane production was shown to be limited both by a lack of additional trace elements as well as by the addition of an overly concentrated trace element mixture. Addition of trace elements at concentrations optimized for standard media enhanced methane production by 37%. After 7 days of incubation, the levels of <italic>mcrA</italic>
transcripts in enrichment cultures with trace element amendment were much higher than in cultures without amendment. Transcript levels of <italic>mcrA</italic>
correlated positively with elevated rates of methane production in supplemented enrichments (<italic>R</italic>
<sup>2</sup>
= 0.95). Metabolically active methanogens, identified by clone sequences of <italic>mcrA</italic>
mRNA retrieved from enrichment cultures, were closely related to <italic>Methanobacterium subterraneum</italic>
and <italic>Methanobacterium formicicum</italic>
. Enrichment cultures were dominated by <italic>M. subterraneum</italic>
and had slightly higher predicted methanogenic richness, but less diversity than enrichment cultures without amendments. These results suggest that varying concentrations of trace elements in produced water from different subsurface coal wells may cause changing levels of CBM production and alter the composition of the active methanogenic community.</p>
</div>
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</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Front Microbiol</journal-id>
<journal-id journal-id-type="iso-abbrev">Front Microbiol</journal-id>
<journal-id journal-id-type="publisher-id">Front. Microbio.</journal-id>
<journal-title-group><journal-title>Frontiers in Microbiology</journal-title>
</journal-title-group>
<issn pub-type="epub">1664-302X</issn>
<publisher><publisher-name>Frontiers Research Foundation</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">22590465</article-id>
<article-id pub-id-type="pmc">3349271</article-id>
<article-id pub-id-type="doi">10.3389/fmicb.2012.00175</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Microbiology</subject>
<subj-group><subject>Original Research</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group><article-title>Trace Elements Affect Methanogenic Activity and Diversity in Enrichments from Subsurface Coal Bed Produced Water</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Ünal</surname>
<given-names>Burcu</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Perry</surname>
<given-names>Verlin Ryan</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
<xref ref-type="author-notes" rid="fn002"><sup>†</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sheth</surname>
<given-names>Mili</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
<xref ref-type="author-notes" rid="fn002"><sup>†</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Gomez-Alvarez</surname>
<given-names>Vicente</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Chin</surname>
<given-names>Kuk-Jeong</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Nüsslein</surname>
<given-names>Klaus</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="author-notes" rid="fn001">*</xref>
</contrib>
</contrib-group>
<aff id="aff1"><sup>1</sup>
<institution>Department of Microbiology, University of Massachusetts</institution>
<country>Amherst, MA, USA</country>
</aff>
<aff id="aff2"><sup>2</sup>
<institution>Department of Biology, Georgia State University</institution>
<country>Atlanta, GA, USA</country>
</aff>
<author-notes><fn fn-type="edited-by"><p>Edited by: Andreas Teske, University of North Carolina at Chapel Hill, USA</p>
</fn>
<fn fn-type="edited-by"><p>Reviewed by: Martin Krüger, Federal Institute for Geosciences and Natural Resources, Germany; Craig Lee Moyer, Western Washington University, USA</p>
</fn>
<corresp id="fn001">*Correspondence: Klaus Nüsslein, Department of Microbiology, University of Massachusetts, 203 Morrill Science Center IVN, 639 North Pleasant Street, Amherst, MA 01003, USA. e-mail: <email>nusslein@microbio.umass.edu</email>
</corresp>
<fn fn-type="other" id="fn002"><p><sup>†</sup>
Verlin Ryan Perry and Mili Sheth have contributed equally to this work.</p>
</fn>
<fn fn-type="other" id="fn003"><p>This article was submitted to Frontiers in Extreme Microbiology, a specialty of Frontiers in Microbiology.</p>
</fn>
</author-notes>
<pub-date pub-type="epub"><day>10</day>
<month>5</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="collection"><year>2012</year>
</pub-date>
<volume>3</volume>
<elocation-id>175</elocation-id>
<history><date date-type="received"><day>16</day>
<month>11</month>
<year>2011</year>
</date>
<date date-type="accepted"><day>20</day>
<month>4</month>
<year>2012</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2012 Ünal, Perry, Sheth, Gomez-Alvarez, Chin and Nüsslein.</copyright-statement>
<copyright-year>2012</copyright-year>
<license license-type="open-access" xlink:href="http://www.frontiersin.org/licenseagreement"><license-p>This is an open-access article distributed under the terms of the <uri xlink:type="simple" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/">Creative Commons Attribution Non Commercial License</uri>
, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.</license-p>
</license>
</permissions>
<abstract><p>Microbial methane from coal beds accounts for a significant and growing percentage of natural gas worldwide. Our knowledge of physical and geochemical factors regulating methanogenesis is still in its infancy. We hypothesized that in these closed systems, trace elements (as micronutrients) are a limiting factor for methanogenic growth and activity. Trace elements are essential components of enzymes or cofactors of metabolic pathways associated with methanogenesis. This study examined the effects of eight trace elements (iron, nickel, cobalt, molybdenum, zinc, manganese, boron, and copper) on methane production, on <italic>mcrA</italic>
transcript levels, and on methanogenic community structure in enrichment cultures obtained from coal bed methane (CBM) well produced water samples from the Powder River Basin, Wyoming. Methane production was shown to be limited both by a lack of additional trace elements as well as by the addition of an overly concentrated trace element mixture. Addition of trace elements at concentrations optimized for standard media enhanced methane production by 37%. After 7 days of incubation, the levels of <italic>mcrA</italic>
transcripts in enrichment cultures with trace element amendment were much higher than in cultures without amendment. Transcript levels of <italic>mcrA</italic>
correlated positively with elevated rates of methane production in supplemented enrichments (<italic>R</italic>
<sup>2</sup>
= 0.95). Metabolically active methanogens, identified by clone sequences of <italic>mcrA</italic>
mRNA retrieved from enrichment cultures, were closely related to <italic>Methanobacterium subterraneum</italic>
and <italic>Methanobacterium formicicum</italic>
. Enrichment cultures were dominated by <italic>M. subterraneum</italic>
and had slightly higher predicted methanogenic richness, but less diversity than enrichment cultures without amendments. These results suggest that varying concentrations of trace elements in produced water from different subsurface coal wells may cause changing levels of CBM production and alter the composition of the active methanogenic community.</p>
</abstract>
<kwd-group><kwd>methanogens</kwd>
<kwd>trace elements</kwd>
<kwd>coal bed methane</kwd>
<kwd><italic>mcrA</italic>
transcript</kwd>
<kwd>enrichments</kwd>
</kwd-group>
<counts><fig-count count="6"></fig-count>
<table-count count="3"></table-count>
<equation-count count="0"></equation-count>
<ref-count count="90"></ref-count>
<page-count count="14"></page-count>
<word-count count="11695"></word-count>
</counts>
</article-meta>
</front>
</pmc>
</record>
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