Responses of primary cultured haemocytes derived from the marine gastropod Haliotis tuberculata to an industrial effluent exposure.
Identifieur interne : 000165 ( Ncbi/Merge ); précédent : 000164; suivant : 000166Responses of primary cultured haemocytes derived from the marine gastropod Haliotis tuberculata to an industrial effluent exposure.
Auteurs : Rim Ladhar-Chaabouni [Tunisie] ; Thomas Houel [France] ; Antoine Serpentini [France] ; Sahar Karray [Tunisie] ; Jean-Marc Lebel [France] ; Amel Hamza-Chaffai [Tunisie]Source :
- Cytotechnology [ 0920-9069 ] ; 2016.
Abstract
This study assessed the responses of primary cultured haemocytes from the marine gastropod Haliotis tuberculata exposed to the increasing concentrations of industrial effluent (0, 0.5, 1, 10, 15 and 20%) discharged into the Tunisian coastal area. Analyses showed the presence of metals such as cadmium (Cd), chromium (Cr), copper (Cu), manganese (Mn), zinc (Zn), nickel (Ni) and lead (Pb) in the effluent. The effects of this mixture of pollutants on abalone haemocyte parameters were reflected by a significant decrease of cell viability, phagocytotic activity and reactive oxygen species (ROS) production as well as morphological and lysosomal membrane alterations. Thus, these results indicated that our primary culture system represents a suitable in vitro model for monitoring of anthropogenic contaminants in aquatic environments.
DOI: 10.1007/s10616-016-0050-7
PubMed: 27957649
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Caen, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Normandie université, 14032, Caen</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Basse-Normandie</region><settlement type="city">Caen</settlement></placeName></affiliation></author><author><name sortKey="Serpentini, Antoine" sort="Serpentini, Antoine" uniqKey="Serpentini A" first="Antoine" last="Serpentini">Antoine Serpentini</name><affiliation wicri:level="3"><nlm:affiliation>Normandie université, 14032, Caen, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Normandie université, 14032, Caen</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Basse-Normandie</region><settlement type="city">Caen</settlement></placeName></affiliation></author><author><name sortKey="Karray, Sahar" sort="Karray, Sahar" uniqKey="Karray S" 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uniqKey="Hamza Chaffai A" first="Amel" last="Hamza-Chaffai">Amel Hamza-Chaffai</name><affiliation wicri:level="1"><nlm:affiliation>Marine Ecotoxicology UR 09-03, IPEIS, BP 805, 3018, Sfax, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Marine Ecotoxicology UR 09-03, IPEIS, BP 805, 3018, Sfax</wicri:regionArea><wicri:noRegion>Sfax</wicri:noRegion></affiliation></author></analytic><series><title level="j">Cytotechnology</title><idno type="ISSN">0920-9069</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study assessed the responses of primary cultured haemocytes from the marine gastropod Haliotis tuberculata exposed to the increasing concentrations of industrial effluent (0, 0.5, 1, 10, 15 and 20%) discharged into the Tunisian coastal area. Analyses showed the presence of metals such as cadmium (Cd), chromium (Cr), copper (Cu), manganese (Mn), zinc (Zn), nickel (Ni) and lead (Pb) in the effluent. The effects of this mixture of pollutants on abalone haemocyte parameters were reflected by a significant decrease of cell viability, phagocytotic activity and reactive oxygen species (ROS) production as well as morphological and lysosomal membrane alterations. Thus, these results indicated that our primary culture system represents a suitable in vitro model for monitoring of anthropogenic contaminants in aquatic environments.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">27957649</PMID><DateCreated><Year>2016</Year><Month>12</Month><Day>13</Day></DateCreated><DateRevised><Year>2016</Year><Month>12</Month><Day>14</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0920-9069</ISSN><JournalIssue CitedMedium="Print"><PubDate><Year>2016</Year><Month>Dec</Month><Day>12</Day></PubDate></JournalIssue><Title>Cytotechnology</Title><ISOAbbreviation>Cytotechnology</ISOAbbreviation></Journal><ArticleTitle>Responses of primary cultured haemocytes derived from the marine gastropod Haliotis tuberculata to an industrial effluent exposure.</ArticleTitle><Pagination><MedlinePgn></MedlinePgn></Pagination><Abstract><AbstractText NlmCategory="UNASSIGNED">This study assessed the responses of primary cultured haemocytes from the marine gastropod Haliotis tuberculata exposed to the increasing concentrations of industrial effluent (0, 0.5, 1, 10, 15 and 20%) discharged into the Tunisian coastal area. Analyses showed the presence of metals such as cadmium (Cd), chromium (Cr), copper (Cu), manganese (Mn), zinc (Zn), nickel (Ni) and lead (Pb) in the effluent. The effects of this mixture of pollutants on abalone haemocyte parameters were reflected by a significant decrease of cell viability, phagocytotic activity and reactive oxygen species (ROS) production as well as morphological and lysosomal membrane alterations. Thus, these results indicated that our primary culture system represents a suitable in vitro model for monitoring of anthropogenic contaminants in aquatic environments.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ladhar-Chaabouni</LastName><ForeName>Rim</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Marine Ecotoxicology UR 09-03, IPEIS, BP 805, 3018, Sfax, Tunisia. rladhar@yahoo.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Houel</LastName><ForeName>Thomas</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Normandie université, 14032, Caen, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>UMR BOREA (Biologie des Organismes et des Ecosystèmes Aquatiques), MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA, Université de Caen Normandie, Esplanade de la Paix, 14032, Caen Cedex 5, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Serpentini</LastName><ForeName>Antoine</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Normandie université, 14032, Caen, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>UMR BOREA (Biologie des Organismes et des Ecosystèmes Aquatiques), MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA, Université de Caen Normandie, Esplanade de la Paix, 14032, Caen Cedex 5, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Karray</LastName><ForeName>Sahar</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Marine Ecotoxicology UR 09-03, IPEIS, BP 805, 3018, Sfax, Tunisia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lebel</LastName><ForeName>Jean-Marc</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Normandie université, 14032, Caen, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>UMR BOREA (Biologie des Organismes et des Ecosystèmes Aquatiques), MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA, Université de Caen Normandie, Esplanade de la Paix, 14032, Caen Cedex 5, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hamza-Chaffai</LastName><ForeName>Amel</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Marine Ecotoxicology UR 09-03, IPEIS, BP 805, 3018, Sfax, Tunisia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>12</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Cytotechnology</MedlineTA><NlmUniqueID>8807027</NlmUniqueID><ISSNLinking>0920-9069</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Haemocytes</Keyword><Keyword MajorTopicYN="N">Haliotis tuberculata</Keyword><Keyword MajorTopicYN="N">Immune parameters</Keyword><Keyword MajorTopicYN="N">In vitro</Keyword><Keyword MajorTopicYN="N">Industrial effluent</Keyword><Keyword MajorTopicYN="N">Primary culture</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>07</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>11</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>12</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>12</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>12</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27957649</ArticleId><ArticleId IdType="doi">10.1007/s10616-016-0050-7</ArticleId><ArticleId IdType="pii">10.1007/s10616-016-0050-7</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li><li>Tunisie</li></country><region><li>Basse-Normandie</li><li>Région Normandie</li></region><settlement><li>Caen</li></settlement></list><tree><country name="Tunisie"><noRegion><name sortKey="Ladhar Chaabouni, Rim" sort="Ladhar Chaabouni, Rim" uniqKey="Ladhar Chaabouni R" first="Rim" last="Ladhar-Chaabouni">Rim Ladhar-Chaabouni</name></noRegion><name sortKey="Hamza Chaffai, Amel" sort="Hamza Chaffai, Amel" uniqKey="Hamza Chaffai A" first="Amel" last="Hamza-Chaffai">Amel Hamza-Chaffai</name><name sortKey="Karray, Sahar" sort="Karray, Sahar" uniqKey="Karray S" first="Sahar" last="Karray">Sahar Karray</name></country><country name="France"><region name="Région Normandie"><name sortKey="Houel, Thomas" sort="Houel, Thomas" uniqKey="Houel T" first="Thomas" last="Houel">Thomas Houel</name></region><name sortKey="Lebel, Jean Marc" sort="Lebel, Jean Marc" uniqKey="Lebel J" first="Jean-Marc" last="Lebel">Jean-Marc Lebel</name><name sortKey="Serpentini, Antoine" sort="Serpentini, Antoine" uniqKey="Serpentini A" first="Antoine" last="Serpentini">Antoine Serpentini</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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