Cells Expressing the RING Finger Z Protein Are Resistant to Arenavirus Infection
Identifieur interne : 000956 ( Main/Exploration ); précédent : 000955; suivant : 000957Cells Expressing the RING Finger Z Protein Are Resistant to Arenavirus Infection
Auteurs : Tatjana I. Cornu ; Heinz Feldmann ; Juan Carlos De La TorreSource :
- Journal of Virology [ 0022-538X ] ; 2004.
Abstract
Arenaviruses include Lassa fever virus (LFV) and the South American hemorrhagic fever viruses. These viruses cause severe human disease, and they pose a threat as agents of bioterrorism. Arenaviruses are enveloped viruses with a bisegmented negative-strand RNA genome whose proteomic capability is limited to four polypeptides: nucleoprotein (NP); surface glycoprotein (GP), which is proteolytically processed into GP1 and GP2; polymerase (L); and a small (11-kDa) RING finger protein (Z). Our investigators have previously shown that Z has a strong inhibitory activity on RNA synthesis mediated by the polymerase of the prototypic arenavirus, lymphocytic choriomeningitis virus (LCMV). In this report we show that cells transduced with a replication-deficient recombinant adenovirus expressing Z (rAd-Z) are resistant to LCMV and LFV infection. Virus cell entry mediated by LCMV or LFV GP was not affected in rAd-Z-transduced cells, but both virus transcription and replication were strongly and specifically inhibited, which resulted in a dramatic reduction in production of infectious virus. These findings open new avenues for developing antiviral strategies to combat the highly pathogenic human arenaviruses, including LFV.
Url:
DOI: 10.1128/JVI.78.6.2979-2983.2004
PubMed: 14990716
PubMed Central: 353761
Affiliations:
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