Mitochondria-independent morphological and biochemical apoptotic alterations promoted by the anti-tumor agent bleomycin in Saccharomyces cerevisiae.
Identifieur interne : 000008 ( Hal/Corpus ); précédent : 000007; suivant : 000009Mitochondria-independent morphological and biochemical apoptotic alterations promoted by the anti-tumor agent bleomycin in Saccharomyces cerevisiae.
Auteurs : Mustapha Aouida ; Halima Mekid ; Omrane Belhadj ; Lluis M. Mir ; Omar TounektiSource :
- Biochemistry and Cell Biology [ 0829-8211 ] ; 2007-02.
Abstract
Bleomycin is a highly potent cytotoxic and genotoxic agent used in the chemotherapy of various types of tumors. It is a radiomimetic anticancer drug that produces single- and double-stranded DNA breaks in a catalytic way. Using Saccharomyces cerevisiae as a model system, we show that when a high amount of bleomycin molecules is internalized (100 micromol/L), morphological changes identical to those usually associated with apoptosis, i.e., a sub-G1 region peak, chromatin condensation, and very rapid DNA fragmentation into oligonucleosomal-sized fragments, are observed. The known bleomycin inhibitors cobalt and EDTA were able to prevent bleomycin nucleasic activity and thus apoptotic cell death. However, both oligomycin, a potent inhibitor of the mitochondrial F0F1-ATPase, and antimycin, a drug affecting mitochondria respiration, were unable to prevent the bleomycin-induced apoptotic-like cell death. These results suggest that high bleomycin concentrations induce an apoptosis-like mitochondria-independent cell death in yeast.
Url:
DOI: 10.1139/o06-147
Links to Exploration step
Hal:hal-00158986Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Mitochondria-independent morphological and biochemical apoptotic alterations promoted by the anti-tumor agent bleomycin in Saccharomyces cerevisiae.</title><author><name sortKey="Aouida, Mustapha" sort="Aouida, Mustapha" uniqKey="Aouida M" first="Mustapha" last="Aouida">Mustapha Aouida</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-1265" status="OLD"><orgName>Vectorologie et transfert de gènes</orgName><orgName type="acronym">VTG / UMR8121</orgName><desc><address><addrLine>Gustave Roussy. 114, rue Édouard-Vaillant. Pavillon de recherche 2 - 3C2 94805 Villejuif Cedex -France. </addrLine><country key="FR"></country></address></desc><listRelation><relation active="#struct-92966" type="direct"></relation><relation name="UMR8121" active="#struct-156104" type="direct"></relation><relation name="UMR8121" active="#struct-441569" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-92966" type="direct"><org type="institution" xml:id="struct-92966" status="VALID"><orgName>Université Paris-Sud - Paris 11</orgName><orgName type="acronym">UP11</orgName><desc><address><addrLine>Bâtiment 300 - 91405 Orsay cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.u-psud.fr/</ref></desc></org></tutelle><tutelle name="UMR8121" active="#struct-156104" type="direct"><org type="institution" xml:id="struct-156104" status="VALID"><orgName>Institut Gustave Roussy</orgName><orgName type="acronym">IGR</orgName><desc><address><addrLine>114, rue Édouard-Vaillant 94805 Villejuif Cedex -France</addrLine><country key="FR"></country></address><ref type="url">http://www.gustaveroussy.fr/</ref></desc></org></tutelle><tutelle name="UMR8121" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno> <idno type="IdRef">02636817X</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc></org></tutelle></tutelles></hal:affiliation></affiliation></author><author><name sortKey="Mekid, Halima" sort="Mekid, Halima" uniqKey="Mekid H" first="Halima" last="Mekid">Halima Mekid</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-1265" status="OLD"><orgName>Vectorologie et transfert de gènes</orgName><orgName type="acronym">VTG / UMR8121</orgName><desc><address><addrLine>Gustave Roussy. 114, rue Édouard-Vaillant. Pavillon de recherche 2 - 3C2 94805 Villejuif Cedex -France. </addrLine><country key="FR"></country></address></desc><listRelation><relation active="#struct-92966" type="direct"></relation><relation name="UMR8121" active="#struct-156104" type="direct"></relation><relation name="UMR8121" active="#struct-441569" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-92966" type="direct"><org type="institution" xml:id="struct-92966" status="VALID"><orgName>Université Paris-Sud - Paris 11</orgName><orgName type="acronym">UP11</orgName><desc><address><addrLine>Bâtiment 300 - 91405 Orsay cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.u-psud.fr/</ref></desc></org></tutelle><tutelle name="UMR8121" active="#struct-156104" type="direct"><org type="institution" xml:id="struct-156104" status="VALID"><orgName>Institut Gustave Roussy</orgName><orgName type="acronym">IGR</orgName><desc><address><addrLine>114, rue Édouard-Vaillant 94805 Villejuif Cedex -France</addrLine><country key="FR"></country></address><ref type="url">http://www.gustaveroussy.fr/</ref></desc></org></tutelle><tutelle name="UMR8121" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno> <idno type="IdRef">02636817X</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc></org></tutelle></tutelles></hal:affiliation></affiliation></author><author><name sortKey="Belhadj, Omrane" sort="Belhadj, Omrane" uniqKey="Belhadj O" first="Omrane" last="Belhadj">Omrane Belhadj</name></author><author><name sortKey="Mir, Lluis M" sort="Mir, Lluis M" uniqKey="Mir L" first="Lluis M" last="Mir">Lluis M. Mir</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-1265" status="OLD"><orgName>Vectorologie et transfert de gènes</orgName><orgName type="acronym">VTG / UMR8121</orgName><desc><address><addrLine>Gustave Roussy. 114, rue Édouard-Vaillant. Pavillon de recherche 2 - 3C2 94805 Villejuif Cedex -France. </addrLine><country key="FR"></country></address></desc><listRelation><relation active="#struct-92966" type="direct"></relation><relation name="UMR8121" active="#struct-156104" type="direct"></relation><relation name="UMR8121" active="#struct-441569" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-92966" type="direct"><org type="institution" xml:id="struct-92966" status="VALID"><orgName>Université Paris-Sud - Paris 11</orgName><orgName type="acronym">UP11</orgName><desc><address><addrLine>Bâtiment 300 - 91405 Orsay cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.u-psud.fr/</ref></desc></org></tutelle><tutelle name="UMR8121" active="#struct-156104" type="direct"><org type="institution" xml:id="struct-156104" status="VALID"><orgName>Institut Gustave Roussy</orgName><orgName type="acronym">IGR</orgName><desc><address><addrLine>114, rue Édouard-Vaillant 94805 Villejuif Cedex -France</addrLine><country key="FR"></country></address><ref type="url">http://www.gustaveroussy.fr/</ref></desc></org></tutelle><tutelle name="UMR8121" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno> <idno type="IdRef">02636817X</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc></org></tutelle></tutelles></hal:affiliation></affiliation></author><author><name sortKey="Tounekti, Omar" sort="Tounekti, Omar" uniqKey="Tounekti O" first="Omar" last="Tounekti">Omar Tounekti</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-1265" status="OLD"><orgName>Vectorologie et transfert de gènes</orgName><orgName type="acronym">VTG / UMR8121</orgName><desc><address><addrLine>Gustave Roussy. 114, rue Édouard-Vaillant. Pavillon de recherche 2 - 3C2 94805 Villejuif Cedex -France. </addrLine><country key="FR"></country></address></desc><listRelation><relation active="#struct-92966" type="direct"></relation><relation name="UMR8121" active="#struct-156104" type="direct"></relation><relation name="UMR8121" active="#struct-441569" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-92966" type="direct"><org type="institution" xml:id="struct-92966" status="VALID"><orgName>Université Paris-Sud - Paris 11</orgName><orgName type="acronym">UP11</orgName><desc><address><addrLine>Bâtiment 300 - 91405 Orsay cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.u-psud.fr/</ref></desc></org></tutelle><tutelle name="UMR8121" active="#struct-156104" type="direct"><org type="institution" xml:id="struct-156104" status="VALID"><orgName>Institut Gustave Roussy</orgName><orgName type="acronym">IGR</orgName><desc><address><addrLine>114, rue Édouard-Vaillant 94805 Villejuif Cedex -France</addrLine><country key="FR"></country></address><ref type="url">http://www.gustaveroussy.fr/</ref></desc></org></tutelle><tutelle name="UMR8121" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno> <idno type="IdRef">02636817X</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc></org></tutelle></tutelles></hal:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">HAL</idno><idno type="RBID">Hal:hal-00158986</idno><idno type="halId">hal-00158986</idno><idno type="halUri">https://hal.archives-ouvertes.fr/hal-00158986</idno><idno type="url">https://hal.archives-ouvertes.fr/hal-00158986</idno><idno type="doi">10.1139/o06-147</idno><date when="2007-02">2007-02</date><idno type="wicri:Area/Hal/Corpus">000008</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Mitochondria-independent morphological and biochemical apoptotic alterations promoted by the anti-tumor agent bleomycin in Saccharomyces cerevisiae.</title><author><name sortKey="Aouida, Mustapha" sort="Aouida, Mustapha" uniqKey="Aouida M" first="Mustapha" last="Aouida">Mustapha Aouida</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-1265" status="OLD"><orgName>Vectorologie et transfert de gènes</orgName><orgName type="acronym">VTG / UMR8121</orgName><desc><address><addrLine>Gustave Roussy. 114, rue Édouard-Vaillant. Pavillon de recherche 2 - 3C2 94805 Villejuif Cedex -France. </addrLine><country key="FR"></country></address></desc><listRelation><relation active="#struct-92966" type="direct"></relation><relation name="UMR8121" active="#struct-156104" type="direct"></relation><relation name="UMR8121" active="#struct-441569" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-92966" type="direct"><org type="institution" xml:id="struct-92966" status="VALID"><orgName>Université Paris-Sud - Paris 11</orgName><orgName type="acronym">UP11</orgName><desc><address><addrLine>Bâtiment 300 - 91405 Orsay cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.u-psud.fr/</ref></desc></org></tutelle><tutelle name="UMR8121" active="#struct-156104" type="direct"><org type="institution" xml:id="struct-156104" status="VALID"><orgName>Institut Gustave Roussy</orgName><orgName type="acronym">IGR</orgName><desc><address><addrLine>114, rue Édouard-Vaillant 94805 Villejuif Cedex -France</addrLine><country key="FR"></country></address><ref type="url">http://www.gustaveroussy.fr/</ref></desc></org></tutelle><tutelle name="UMR8121" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno> <idno type="IdRef">02636817X</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc></org></tutelle></tutelles></hal:affiliation></affiliation></author><author><name sortKey="Mekid, Halima" sort="Mekid, Halima" uniqKey="Mekid H" first="Halima" last="Mekid">Halima Mekid</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-1265" status="OLD"><orgName>Vectorologie et transfert de gènes</orgName><orgName type="acronym">VTG / UMR8121</orgName><desc><address><addrLine>Gustave Roussy. 114, rue Édouard-Vaillant. Pavillon de recherche 2 - 3C2 94805 Villejuif Cedex -France. </addrLine><country key="FR"></country></address></desc><listRelation><relation active="#struct-92966" type="direct"></relation><relation name="UMR8121" active="#struct-156104" type="direct"></relation><relation name="UMR8121" active="#struct-441569" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-92966" type="direct"><org type="institution" xml:id="struct-92966" status="VALID"><orgName>Université Paris-Sud - Paris 11</orgName><orgName type="acronym">UP11</orgName><desc><address><addrLine>Bâtiment 300 - 91405 Orsay cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.u-psud.fr/</ref></desc></org></tutelle><tutelle name="UMR8121" active="#struct-156104" type="direct"><org type="institution" xml:id="struct-156104" status="VALID"><orgName>Institut Gustave Roussy</orgName><orgName type="acronym">IGR</orgName><desc><address><addrLine>114, rue Édouard-Vaillant 94805 Villejuif Cedex -France</addrLine><country key="FR"></country></address><ref type="url">http://www.gustaveroussy.fr/</ref></desc></org></tutelle><tutelle name="UMR8121" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno> <idno type="IdRef">02636817X</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc></org></tutelle></tutelles></hal:affiliation></affiliation></author><author><name sortKey="Belhadj, Omrane" sort="Belhadj, Omrane" uniqKey="Belhadj O" first="Omrane" last="Belhadj">Omrane Belhadj</name></author><author><name sortKey="Mir, Lluis M" sort="Mir, Lluis M" uniqKey="Mir L" first="Lluis M" last="Mir">Lluis M. Mir</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-1265" status="OLD"><orgName>Vectorologie et transfert de gènes</orgName><orgName type="acronym">VTG / UMR8121</orgName><desc><address><addrLine>Gustave Roussy. 114, rue Édouard-Vaillant. Pavillon de recherche 2 - 3C2 94805 Villejuif Cedex -France. </addrLine><country key="FR"></country></address></desc><listRelation><relation active="#struct-92966" type="direct"></relation><relation name="UMR8121" active="#struct-156104" type="direct"></relation><relation name="UMR8121" active="#struct-441569" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-92966" type="direct"><org type="institution" xml:id="struct-92966" status="VALID"><orgName>Université Paris-Sud - Paris 11</orgName><orgName type="acronym">UP11</orgName><desc><address><addrLine>Bâtiment 300 - 91405 Orsay cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.u-psud.fr/</ref></desc></org></tutelle><tutelle name="UMR8121" active="#struct-156104" type="direct"><org type="institution" xml:id="struct-156104" status="VALID"><orgName>Institut Gustave Roussy</orgName><orgName type="acronym">IGR</orgName><desc><address><addrLine>114, rue Édouard-Vaillant 94805 Villejuif Cedex -France</addrLine><country key="FR"></country></address><ref type="url">http://www.gustaveroussy.fr/</ref></desc></org></tutelle><tutelle name="UMR8121" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno> <idno type="IdRef">02636817X</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc></org></tutelle></tutelles></hal:affiliation></affiliation></author><author><name sortKey="Tounekti, Omar" sort="Tounekti, Omar" uniqKey="Tounekti O" first="Omar" last="Tounekti">Omar Tounekti</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-1265" status="OLD"><orgName>Vectorologie et transfert de gènes</orgName><orgName type="acronym">VTG / UMR8121</orgName><desc><address><addrLine>Gustave Roussy. 114, rue Édouard-Vaillant. Pavillon de recherche 2 - 3C2 94805 Villejuif Cedex -France. </addrLine><country key="FR"></country></address></desc><listRelation><relation active="#struct-92966" type="direct"></relation><relation name="UMR8121" active="#struct-156104" type="direct"></relation><relation name="UMR8121" active="#struct-441569" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-92966" type="direct"><org type="institution" xml:id="struct-92966" status="VALID"><orgName>Université Paris-Sud - Paris 11</orgName><orgName type="acronym">UP11</orgName><desc><address><addrLine>Bâtiment 300 - 91405 Orsay cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.u-psud.fr/</ref></desc></org></tutelle><tutelle name="UMR8121" active="#struct-156104" type="direct"><org type="institution" xml:id="struct-156104" status="VALID"><orgName>Institut Gustave Roussy</orgName><orgName type="acronym">IGR</orgName><desc><address><addrLine>114, rue Édouard-Vaillant 94805 Villejuif Cedex -France</addrLine><country key="FR"></country></address><ref type="url">http://www.gustaveroussy.fr/</ref></desc></org></tutelle><tutelle name="UMR8121" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno> <idno type="IdRef">02636817X</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc></org></tutelle></tutelles></hal:affiliation></affiliation></author></analytic><idno type="DOI">10.1139/o06-147</idno><series><title level="j">Biochemistry and Cell Biology</title><idno type="ISSN">0829-8211</idno><imprint><date type="datePub">2007-02</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Bleomycin is a highly potent cytotoxic and genotoxic agent used in the chemotherapy of various types of tumors. It is a radiomimetic anticancer drug that produces single- and double-stranded DNA breaks in a catalytic way. Using Saccharomyces cerevisiae as a model system, we show that when a high amount of bleomycin molecules is internalized (100 micromol/L), morphological changes identical to those usually associated with apoptosis, i.e., a sub-G1 region peak, chromatin condensation, and very rapid DNA fragmentation into oligonucleosomal-sized fragments, are observed. The known bleomycin inhibitors cobalt and EDTA were able to prevent bleomycin nucleasic activity and thus apoptotic cell death. However, both oligomycin, a potent inhibitor of the mitochondrial F0F1-ATPase, and antimycin, a drug affecting mitochondria respiration, were unable to prevent the bleomycin-induced apoptotic-like cell death. These results suggest that high bleomycin concentrations induce an apoptosis-like mitochondria-independent cell death in yeast.</div></front></TEI><hal api="V3"><titleStmt><title xml:lang="en">Mitochondria-independent morphological and biochemical apoptotic alterations promoted by the anti-tumor agent bleomycin in Saccharomyces cerevisiae.</title><author role="aut"><persName><forename type="first">Mustapha</forename><surname>Aouida</surname></persName><email></email><idno type="halauthor">126894</idno><affiliation ref="#struct-1265"></affiliation></author><author role="aut"><persName><forename type="first">Halima</forename><surname>Mekid</surname></persName><email></email><idno type="halauthor">126896</idno><affiliation ref="#struct-1265"></affiliation><affiliation ref="#struct-2798"></affiliation></author><author role="aut"><persName><forename type="first">Omrane</forename><surname>Belhadj</surname></persName><email></email><idno type="halauthor">191580</idno></author><author role="aut"><persName><forename type="first">Lluis M</forename><surname>Mir</surname></persName><email></email><idno type="halauthor">191581</idno><affiliation ref="#struct-1265"></affiliation></author><author role="aut"><persName><forename type="first">Omar</forename><surname>Tounekti</surname></persName><email></email><idno type="halauthor">126895</idno><affiliation ref="#struct-1265"></affiliation><affiliation ref="#struct-19952"></affiliation></author><editor role="depositor"><persName><forename>Michel</forename><surname>Perricaudet</surname></persName><email>perricau@igr.fr</email></editor></titleStmt><editionStmt><edition n="v1" type="current"><date type="whenSubmitted">2007-07-02 10:29:15</date><date type="whenModified">2016-09-28 16:20:13</date><date type="whenReleased">2007-07-02 10:29:15</date><date type="whenProduced">2007-02</date></edition><respStmt><resp>contributor</resp><name key="103228"><persName><forename>Michel</forename><surname>Perricaudet</surname></persName><email>perricau@igr.fr</email></name></respStmt></editionStmt><publicationStmt><distributor>CCSD</distributor><idno type="halId">hal-00158986</idno><idno type="halUri">https://hal.archives-ouvertes.fr/hal-00158986</idno><idno type="halBibtex">aouida:hal-00158986</idno><idno type="halRefHtml">Biochemistry and Cell Biology, NRC Research Press, 2007, 85 (1), pp.49-55. <10.1139/o06-147></idno><idno type="halRef">Biochemistry and Cell Biology, NRC Research Press, 2007, 85 (1), pp.49-55. <10.1139/o06-147></idno></publicationStmt><seriesStmt><idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno><idno type="stamp" n="CDF">Collège de France</idno><idno type="stamp" n="UNIV-PSUD">Université Paris Sud - Paris XI</idno><idno type="stamp" n="UNIV-PARIS11">Université Paris-Sud 11</idno><idno type="stamp" n="FNCLCC" p="INSERM">UNICANCER</idno><idno type="stamp" n="IGR" p="FNCLCC">Institut Gustave Roussy</idno><idno type="stamp" n="PSL">Paris Sciences et Lettres (PSL) Research University</idno></seriesStmt><notesStmt><note type="audience" n="1">Not set</note><note type="popular" n="0">No</note><note type="peer" n="1">Yes</note></notesStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Mitochondria-independent morphological and biochemical apoptotic alterations promoted by the anti-tumor agent bleomycin in Saccharomyces cerevisiae.</title><author role="aut"><persName><forename type="first">Mustapha</forename><surname>Aouida</surname></persName><idno type="halAuthorId">126894</idno><affiliation ref="#struct-1265"></affiliation></author><author role="aut"><persName><forename type="first">Halima</forename><surname>Mekid</surname></persName><idno type="halAuthorId">126896</idno><affiliation ref="#struct-1265"></affiliation><affiliation ref="#struct-2798"></affiliation></author><author role="aut"><persName><forename type="first">Omrane</forename><surname>Belhadj</surname></persName><idno type="halAuthorId">191580</idno></author><author role="aut"><persName><forename type="first">Lluis M</forename><surname>Mir</surname></persName><idno type="halAuthorId">191581</idno><affiliation ref="#struct-1265"></affiliation></author><author role="aut"><persName><forename type="first">Omar</forename><surname>Tounekti</surname></persName><idno type="halAuthorId">126895</idno><affiliation ref="#struct-1265"></affiliation><affiliation ref="#struct-19952"></affiliation></author></analytic><monogr><idno type="halJournalId" status="VALID">3430</idno><idno type="issn">0829-8211</idno><idno type="eissn">1208-6002</idno><title level="j">Biochemistry and Cell Biology</title><imprint><publisher>NRC Research Press</publisher><biblScope unit="volume">85</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="pp">49-55</biblScope><date type="datePub">2007-02</date></imprint></monogr><idno type="doi">10.1139/o06-147</idno><idno type="pubmed">17464344</idno></biblStruct></sourceDesc><profileDesc><langUsage><language ident="en">English</language></langUsage><textClass><classCode scheme="halDomain" n="sdv.bbm.bc">Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]</classCode><classCode scheme="halTypology" n="ART">Journal articles</classCode></textClass><abstract xml:lang="en">Bleomycin is a highly potent cytotoxic and genotoxic agent used in the chemotherapy of various types of tumors. It is a radiomimetic anticancer drug that produces single- and double-stranded DNA breaks in a catalytic way. Using Saccharomyces cerevisiae as a model system, we show that when a high amount of bleomycin molecules is internalized (100 micromol/L), morphological changes identical to those usually associated with apoptosis, i.e., a sub-G1 region peak, chromatin condensation, and very rapid DNA fragmentation into oligonucleosomal-sized fragments, are observed. The known bleomycin inhibitors cobalt and EDTA were able to prevent bleomycin nucleasic activity and thus apoptotic cell death. However, both oligomycin, a potent inhibitor of the mitochondrial F0F1-ATPase, and antimycin, a drug affecting mitochondria respiration, were unable to prevent the bleomycin-induced apoptotic-like cell death. These results suggest that high bleomycin concentrations induce an apoptosis-like mitochondria-independent cell death in yeast.</abstract></profileDesc></hal></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/CobaltMaghrebV1/Data/Hal/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000008 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Hal/Corpus/biblio.hfd -nk 000008 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Terre
|area= CobaltMaghrebV1
|flux= Hal
|étape= Corpus
|type= RBID
|clé= Hal:hal-00158986
|texte= Mitochondria-independent morphological and biochemical apoptotic alterations promoted by the anti-tumor agent bleomycin in Saccharomyces cerevisiae.
}}
| This area was generated with Dilib version V0.6.32. |  |